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Alzheimer’s disease, neural stem cells and neurogenesis: cellular phase at single-cell level

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Neural Regeneration Research 2020年第5期15卷 824-827页

作者： Mehmet Ilyas Cosacak Prabesh Bhattarai Caghan Kizil German center for Neurodegenerative Diseases (DZNE) Dresden Helmholtz AssociationDresdenGermany center for molecular and Cellular bioengineering (CMCB) CRTDTU DresdenDresdenGermany

Alzheimer’s disease cannot be cured as of *** current understanding on the causes of Alzheimer’s disease is *** develop treatments,experimental models that represent a particular cellular phase of the disease and more rigorous scrutiny of the cellular pathological mechanisms are *** recent years,Alzheimer’s disease research underwent a paradigm *** to this tendency,Alzheimer’s disease is increasingly being conceived of a disease where not only neurons but also multiple cell types synchronously partake to manifest the *** on every cell type adds an alternative approach and hope for the efforts towards the *** stem cells and their neurogenic ability are making an appearance as a new aspect of the disease manifestation based on the recent findings that neurogenesis reduces dramatically in Alzheimer’s disease patients compared to healthy ***,understanding how neural stem cells can form new neurons in Alzheimer’s disease brains holds an immense potential for ***,this provocative idea requires further evidence and tools for ***,single cell sequencing appeared as a revolutionary tool to understand cellular programs in unprecedented resolution and it will undoubtedly facilitate comprehensive investigation of different cell types in Alzheimer’s *** this mini-review,we will touch upon recent studies that use single cell sequencing for investigating cellular response in Alzheimer’s disease and some consideration pertaining to the utilization of neural regeneration for Alzheimer’s disease research.

关键词： Alzheimer's disease mouse neural regeneration neural stem cell neurogenesis neuron single cell sequencing zebrafish

Melt Synthesis and Characterization of Poly(L-lactic Acid) Chain Linked by Multifunctional Epoxy Compound

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Journal of Wuhan University of Technology(Materials Science) 2010年第5期25卷 774-779页

作者：王远亮 Research center of Bioinspired Materials Science and Engineering Bioengineering College Chongqing University

High molecular weight（Mw） poly（L-lactic acid）s（PLLAs） were synthesized using multifunctional epoxy compound（Joncryl-ADR4370） as chain extender. The products were characterized by gel permeation chromatography（GPC） and spectroscopy（1HNMR and FTIR）. The results indicated that the Mw of PLLA increased with the increasing of the ratio of epoxy compound and the extending of reaction time. The highest Mw of PLLA reached 360 000 g/mol when the ratio of epoxy compound was 1.5 wt%. However, the reactants turned to cross-linking when the ratio of epoxy compound was over 1.5 wt%. Differential scanning calorimetry（DSC） measurements demonstrated that the glass transition（Tg） and melting temperatures（Tm） of products increased slightly as the increase of the molecular weight. Analysis of the hydrolytic degradation in vitro showed that the branched PLLA possessed the quicker degradability than that of the linear PLLA.

关键词： melt synthesis poly(lactic acid) epoxy compound chain extender

Depression mechanism of sulfite ions on sphalerite and Pb^(2+)activated sphalerite in the flotation separation of galena from sphalerite

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 335-345页

作者： Feng Zhang Chenyang Zhang Linlin Wu Wei Sun Hongliang Zhang Jianhua Chen Yong Pei Songjiang Li Engineering Research center of Ministry of Education for Carbon Emission Reduction in Metal Resource Exploitation and Utilization Hunan International Joint Research Center for Efficient and Clean Utilization of Critical Metal Mineral ResourcesSchool of Minerals Processing and BioengineeringCentral South UniversityChangsha 410083China State Key Laboratory of Mineral Processing Beijing General Research Institute of Mining&MetallurgyBeijing 100160China Hunan Provincial Key Laboratory of Micro&Nano Materials Interfaces Science College of Chemistry and Chemical EngineeringCentral South UniversityChangsha 410083China School of Resources Environment and MaterialsGuangxi UniversityNanning 530004China Department of Chemistry Xiangtan UniversityXiangtan 411105China China Railway Resource Group Co.Ltd Beijing 102300China

The depression mechanism of sulfite ions on sphalerite and Pb^(2+)activated sphalerite in the flotation separation of galena from sphalerite still lacked in-depth ***,the depression mechanism of sulfite ions on sphalerite and Pb^(2+)activated sphalerite in the flotation separation of galena from sphalerite was further systematically investigated with experiments and density functional theory(DFT)*** X-ray photoelectric spectroscopy(XPS)results,DFT calculation results,and frontier molecular orbital analysis indicated that sulfite ions were difficult to be adsorbed on sphalerite surface,suggesting that sulfite ions achieved depression effects on sphalerite through other non-adsorption ***,the oxygen content in the surface of sphalerite treated with sulfite ions in creased,which enhanced the hydrophilicity of the sphalerite and further increased the difference in hydrophilicity between sphalerite and ***,sulfite ions were chelated with lead ions to form PbSO_(3)in *** hydrophilic PbSO_(3)was more easily adsorbed on sphalerite than *** interaction between sulfite ions and lead ions could effectively inhibit the activation of *** addition the UV spectrum showed that after adding sulfite ions,the peak of perxanthate in the sphalerite treated xanthate solution was significantly stronger than that in the galena with xanthate solution,indicating that xanthate interacted more readily with sulfite ions and oxygen mo lecules within the sphalerite system,leading to the formation of ***,sulfite ions hardly depressed the flotation of ga lena and could promote the flotation of galena to some *** study deepened the understanding of the depression mechanism o sulfite ions on sphalerite and Pb^(2+)activated sphalerite.

关键词： sphalerite galena sulfite ion density functional theory depression mechanism

Characteristics,formation mechanism and stability of high internal phase emulsions stabilized by porcine plasma protein (PPP) / carrageenan (CG) hybrid particles

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Food Bioscience 2022年第3期47卷 1132-1141页

作者： Ping Yan Jiang-lan Yuan Xu Kang Jin-ying Lv Xiao-cui Liu National “111” center for Cellular Regulation and molecular Pharmaceutics Key Laboratory of Fermentation Engineering (Ministry of Education)College of Bioengineering and FoodHubei University of TechnologyWuhan430068China

High internal phase emulsions (HIPEs) stabilized by nanoparticles based on biomacromolecules are challenging issues in recent ***,a newly developed HIPE was investigated by using heat-denatured porcine plasma protein (PPP) nanoparticles at pH 6.5 as emulsifier,and its emulsifying stability could be significantly enhanced by compounding carrageenan (CG).In the miscible system,PPP and CG formed hybrid particles through non-covalent interaction,and the sizes and zeta-potentials of the particles increased significantly along with addition of CG (from 0 to 0.7%,w/v),reached up to about 3.6 μm and −53 mV at 0.5% (w/v),*** weakened the ability of PPP to lower interfacial tension of oil/water (O/W),but increased the apparent viscosity of the *** results from CLSM,rheology and stability experiments indicated a significant increasing trend of the HIPEs stability and solid-like characteristics along with addition of *** with the controls including bovine serum albumin (BSA),BSA-CG and CG alone,PPP-CG hybrid particles had good performance in fabricating and stabilizing the *** work revealed the novel function of PPP as emulsifier of HIPEs and so offered the theoretical direction for application of PPP as a mass by-product,as well as an excellent HIPEs system for food,medicine and cosmetics fields.

关键词： Porcine plasma protein Carrageenan Nanoparticles High internal phase emulsion Characteristics Stability

Protective,controlled-release and embedding mechanism of porcine plasma protein cold-set gel on quercetin:An effective carrier of hydrophobic compounds

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Food Bioscience 2022年第3期47卷 625-634页

作者： Jiang-lan Yuan Chang-sheng Ding Cheng-liang Li Yu Zhang Xu Kang National “111” center for Cellular Regulation and molecular Pharmaceutics Key Laboratory of Fermentation Engineering (Ministry of Education)College of Bioengineering and FoodHubei University of TechnologyWuhan430068China

Protection and embedding of hydrophobic bioactive compounds using protein hydrogels are emerging focus during the latest *** present study,we fabricated the porcine plasma protein(PPP)cold-set gel by microbial transglutaminase(MTGase)and glucono-δ-lactone(GDL)as coupling *** a result,the embedding,protection and controlled-release effect of the gel on vulnerable hydrophobic bioactive components(quercetin(Que)as representative)with proposed molecular mechanisms were investigated in *** results showed that high concentration of Que(5 mmol/L)could be loaded with PPP cold-set gel and embedding efficiency(EE)was over 98%.Compared with free Que,the embedded one exhibited significantly higher thermostability,photochemical stability and storage stability(P<0.05).In addition,the gel loaded with 5 mmol L^(−1) of Que had higher swelling potency under gastric(low pH)media and controlled release performance following simulated intestinal digestive *** holding capacity(WHC)implied that free water molecules played less role in the retention ability of Que in fabricated gel ***-assisted structural characterization proved that Que was efficiently embedded mainly by generating PPP-Que complex through hydrogen bond and van der Waals force,and the binding site was mainly near Trp *** work gave novel insight into the potential use of PPP cold-set gel as an excellent carrier towards protection and selective delivery of vulnerable small hydrophobic nutraceutical compounds.

关键词： Porcine plasma protein Cold-set gel Quercetin Embedding Interaction Controlled-release

The impact of N-terminal phosphorylation on LHCII conformation in state transition

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Acta Mechanica Sinica 2014年第3期30卷 447-456页

作者： Jin-Hong Ding Ning Li Man-Liu Wang Yan Zhang Shou-Qin Lü Mian Long Key Laboratory of Microgravity(National Microgravity Laboratory)and center of Biomechanics and bioengineering Institute of MechanicsChinese Academy of Sciences

State transition is an important protection mechanism of plants for maintaining optimal efficiency through redistributing unbalanced excitation energy between photosystem II （PSII） and photosystem I （PSI）. This process depends on the reversible phosphorylation/dephosphorylation of the major light-harvesting complex II （LHCII） and its bi-directional migration between PSII and PSI. But it remains unclear how phosphorylation/dephosphorylation modulates the LHCII conformation and further regulates its reversible migration. Here molecular dynamics simulations （MDS） were employed to elucidate the impact of phosphorylation on LHCII conformation. The results indicated that N-terminal phosphorylation loosened LHCII trimer with decreased hydrogen bond （H-bond） interactions and extended the distances between neighboring monomers, which stemmed from the conformational ad- justment of each monomer itself. Global conformational change of LHCII monomer started from its stromal N- terminal （including the phosphorylation sites） by enhancing its interaction to lipid membrane and by adjusting the interaction network with surrounded inter-monomer andintra-monomer transmembrane helixes of B, C, and A, and finally triggered the reorientation of transmembrane helixes and transferred the conformational change to luminal side helixes and loops. These results further our understanding in molecular mechanism of LHCII migration during state transition from the phosphorylation-induced microstructural feature of LHCII.

关键词： State transition LHCII Phosphorylation Conformation

Carbonaceous Nanofibers-titanium Dioxide Nanocomposites: Synthesis and Use as a Platform for Removal of Dye Pollutants

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Journal of Wuhan University of Technology(Materials Science) 2019年第2期34卷 303-307页

作者：郭洪影 JIANG Shan WANG Chaoyuan LI Shenwan FENG Jianjian 孙红梅 ZHU Hongda GUO Huiling LIU Mingxing SUN Honghao School of bioengineering and Food Key Laboratory of Fermentation Engineering (Ministry of Education) Key Laboratory of Industrial Microbiology in Hubei National ‘‘111'' Center for Cellular Regulation and Molecular Pharmaceutics Hubei Provincial Cooperative Innovation Center of Industrial Fermentation Hubei University of Technology

A mild chemistry route was developed to prepare carbonaceous nanofibers-titanium dioxide(CNF-TiO_2) nanocomposites for removal of dye pollutants. In the process of the template-directed hydrothermal carbonization(HTC), ultrathin Te nanowires were adopted as templates and glucose as the carbon source, and TiO_2 was decorated on CNF via the hydrolysis of tetrabutyltitanate in the presence of CNF in ethanol. The as-prepared materials were characterized by scanning electron microscopy(SEM), transmission electron microscopy(TEM), energy-dispersive X-ray(EDX) and X-ray diffraction(XRD). SEM and TEM observations displayed that TiO_2 nanoparticles were anchored on the CNF. EDX and XRD data confirmed that the assynthesized samples were CNF-TiO_2, and TiO_2 belonged to anatase titania. Taking advantage of combined benefits of carbonaceous nanofibers and titanium dioxide, these CNF-TiO_2 nanocomposites exhibited higher removal efficiency in a short time and showed good reusability. It was showed that over 97% of Rhodamine B could be removed in 15 min without generating the solid and liquid wastes. The removal efficiency of dyes was still over 80% after reuse in five cycles. All the results demonstrate that the as-prepared CNF-TiO_2 composites are effective materials for fast and effective removal of dye pollutants and thus can provide a new platform for dye decontamination.

关键词： carbon materials nanocomposites dye pollutants

Metal-organic frameworks nanoswitch:Toward photo-controllable endo/lysosomal rupture and release for enhanced cancer RNA interference

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Nano Research 2020年第1期13卷 238-245页

作者： Gan Lin Yang Zhang Long Zhang Junqing Wang Ye Tian Wen Cai Shangui Tang Chengchao Chu JiaJing Zhou Peng Mi Xiaoyuan Chen Gang Liu State Key Laboratory of molecular Vaccinology and molecular Diagnostics&center for molecular Imaging and Translational Medicine School of Public HealthXiamen UniversityXiamen361102China The Department of Chemical Engineering The University of MelbourneParkvilleVictoria3010Australia School of Chemical and Biomedical Engineering Nanyang Technological UniversitySingapore637457Singapore State Key Laboratory of Biotherapy and Cancer center West China HospitalCollaborative Innovation Center for BiotherapySichuan UniversityChengdu610041China Laboratory of molecular Imaging and Nanomedicine National Institute of Biomedical Imaging and BioengineeringNational Institutes of HealthBethesdaMD20892USA

Endo/lysosomal escape and gene release are two critical bottlenecks in gene ***,a novel photo-controllable metal-organic frameworks(MOFs)nanoswitch is rationally designed for enhancing small interfering RNA(siRNA)*** single laser triggers the“off-to-on”switching of MOFs nanocomplexes,inducing significant siRNA release accompanied by rapid MOFs dissociation into protonatable 2-methylimidazalo and osmotic rupturing Zn2+ions,which cooperatively contribute to remarkable endo/lysosomal rupture(∼90%).The simultaneous endo/lysosomal rupture and release enable a high spatio-temporal control on RNA interference for effective cancer ***,the“off-to-on”switching also activates fluorescence recovery for real-time monitoring siRNA *** nanoswitch could easily be extended to deliver other therapeutic agents(e.g.,DNA,protein,anticancer drug)for overcoming endo/lysosomal entrapment.

关键词： metal-organic frameworks endo/lysosomal rupture and release photo-controllable nanoswitch RNA interference cancer therapy

MNDO research on alkylation of DNA bases by the non-α-position metabolite of 3, 5-dimethyl-N-nitrosopeperazine

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Chinese Science Bulletin 1997年第8期42卷 698-700页

作者： ZHONG Rugang and DAI Qianhuancenter for Cancer Chemistry and bioengineering, Beijing Polytechnic University, Beijing 100022. China center for Cancer Chemistry and bioengineering Beijing Polytechnic IJniversity Beijing China

IT is a commonly accepted concept that having formed alkyldiazonium ions after α-hydroxyla-tion,the metabolites of N-nitroso compounds(NNCs)combine with DNA and initiate *** the complex relationship between carcinoge... 详细信息

关键词： nitrosopeperazine di-region theory alkylation of DNA MNDO.