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Post-Translational Regulation of AtFER2 Ferritin in Response to Intracellular Iron Trafficking during Fruit Development in Arabidopsis

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Molecular Plant 2009年第5期2卷 1095-1106页

作者： Karl Ravet brigitte Touraine Sun A. Kim Francoise Cellier Sebastien Thomine Mary Lou Guerinot Jean-Francois briat Frederic Gaymard biochimie et Physiologie Moleculaire des Plantes (b&pmp) Unite Mixte de Recherche CNRS INRA Universite Montpellier 2 SupAgro Place Viala Bat. 7 F-34060 Montpellier France Department of biological Sciences Dartmouth College 6044 Gilman Hall Hanover NH 03755 USA Institut des Sciences du Vegetal CNRS Bat 23 Avenue de la Terrasse 91198 Gif-sur-Yvette France

Ferritins are major players in plant iron homeostasis. Surprisingly, their overexpression in transgenic plants led only to a moderate increase in seed iron content, suggesting the existence of control checkpoints for iron loading and storage in seeds. This work reports the identification of two of these checkpoints. First, measurement of seed metal content during fruit development in Arabidopsis thaliana reveals a similar dynamic of loading for Fe, Mn, Cu, and Zn. The step controlling metal loading into the seed occurs by the regulation of transport from the hull to the seed. Second, metal loading and ferritin abundance were monitored in different genetic backgrounds affected in vacuolar iron transport （AtVIT1, AtNRAMP3, AtNRAMP4） or plastid iron storage （AtFER1 to 4）. This approach revealed （1） a post-translational reg- ulation of ferritin accumulation in seeds, and （2） that ferritin stability depends on the balance of iron allocation between vacuoles and plastids. Thus, the success of ferritin overexpression strategies for iron biofortification, a promising approach to reduce iron-deficiency anemia in developing countries, would strongly benefit from the identification and engineering of mechanisms enabling the translocation of high amounts of iron into seed plastids.

关键词： Nutrient and metal transport iron transport ferritin post-transcriptional control biofortification Arabidopsis.

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iPlant Systems biology (iPSb): An International Network Hub in the Plant Community

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Molecular Plant 2019年第6期12卷 727-730页

作者： Pascal Falter-braun Siobhan brady Rodrigo A.Gutierrez Gloria M.Coruzzi Gabriel Krouk Institute of Network biology (INET) Helmholtz Zentrum Munchen (HMGU) 85764 Munchen-Neuherberg Germany Microbe-Host Interactions Faculty of Biology. Ludwig-Maximilians-University (LMU) Munich 82152 Planegg-Martinsried Germany Department of Plant biology and the Genome Center University of California Davis CA 95616 USA Departamento de Genetica Molecular y Microbiologia FONDAP Center for Genome Regulation Millennium Institute for Integrative Biology Pontificia Universidad Catolica de Chile Santiago 8331150 Chile Center for Genomics and Systems biology Department of Biology New York University New York NY 10003 USA b&pmp Univ Montpellier CNRS INRA SupAgro Montpellier France

sequenced genome of a multicellular organism (Arabidopsis Genome, 2000) completed just after those of the nematode Caenorhabditis elegans (C. elegans Sequencing Consortium, 1998) and the fruit fly Drosophila melanogaster (Adams et al., 2000). Availability of a full whole-genome sequence for the reference plant opened to plant biologists what is commonly called the post-genomic era.

关键词： Systems biology International Network Hub Plant Community

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Transformation and Expression of Choline Monooxygenase(CMO) Gene in Embryogenic Tissue of White Pine(Pinus strobus)

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Journal of Shanghai Jiaotong university(Science) 2005年第S1期10卷 38-44,54页

作者：范建芝申晓辉蒋湘宁 YILL Sung Park M K. Mahendrappa School of Agriculture and biology Shanghai Jiaotong Univ. Shanghai 201101 China School of Life Science and Technology Beijing Forestry Univ. Beijng 100083 Canadian Forest Service -Atlantic Forestry Center P. O. box 4000 Fredericton NB E3B 5P7 Canada

A transformation procedure of choline monooxygenase(CMO) gene, involved in stress tolerance, was established in white pine embryogenic tissue by using A. tumefaciens C58/pmp90. The CMO cDNA fragment(1.3 kb) was generated by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) with primers based on the report sequence of CMO in gene bank. A chimerical gene composed of the cauliflower mosaic virus (CaMV) 35S promoter fused to CMO cDNA and β-glucuronidase (GUS-marker gene) was transferred into Ti-derived disarmed binary vector pbI121. The new vector, p35SCMOp, was transferred into Agrobacterium tumefaciens C58/pmp90 by freeze-thaw method. Somatic embryogenesis (SE) initiation of Pinus. Strobus L. and *** Sieb. et Zucc. depended on the manipulation of plant growth regulator (PGR) concentrations in the GLH culture medium. Transgenic embryos and regenerated plants of two Pine species were produced after co-culture of embryogenic tissue with the disarmed strain of A. tumefaciens C58/pmp90/ p35SCMOp and selected on medium containing 25mg/L kanamycin. The transformed embryogenic tissue was initially confirmed by histochemical GUS assay followed by PCR. One copy of T-DNA was detected by transgenic lines analysis in Pinus. Strobus L. and transgenic plants were regenerated for two species using modified protocols for maturation and germination of somatic embryos.

关键词： choline monooxygenase (CMO) transformation expression Pinus strobus L. embryogenic tissue

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高温超导YbCO单晶畴生长中的过冷度和生长机制

高温超导YBCO单晶畴生长中的过冷度和生长机制

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第十届全国磁悬浮技术与振动控制学术会议(CSMLTVC10)

作者：张翠萍梁朝晖 Xavier Chaud Eric beaugnon A.L.Solovjov 许吉敏赵志明杨芳王庆阳王国栋韩吉庆李高山贾佳林西北有色金属研究院超导材料所西部超导材料科技股份有限公司 CRETA/CNRS b.Verkin Institute for Low Temperature Physics and Engineering National Academy of Sciences of Ukraine 合肥工业大学机械工程学院摩擦研究所陕西科技大学机电工程系西北有色金属研究院难熔金属研究所西北有色金属研究院贵金属研究所

我们采用粉末融化法(pmp)制备了YbCO单晶畴块材。通过淬火实验、热重测试观察,研究了pmp-YbCO单晶畴的生长动力学。通过生长速率测试,在pmp-YbCO单晶畴生长中发现存在一个过冷度的分界点ΔT′。当ΔTΔT′时,R和R随着过冷度的增大而减... 详细信息

我们采用粉末融化法(pmp)制备了YbCO单晶畴块材。通过淬火实验、热重测试观察,研究了pmp-YbCO单晶畴的生长动力学。通过生长速率测试,在pmp-YbCO单晶畴生长中发现存在一个过冷度的分界点ΔT′。当ΔTΔT′时,R和R随着过冷度的增大而减小。对于ab面的生长速率R,ΔT′≈10～18 K,对于c轴方向的生长速率R,ΔT′≈20K。我们的实验证实这一生长速率随过冷度增大而降低的现象是氧扩散造成的。当氧气饱和度低而且扩散缓慢时,即使过冷度增加,生长速率也不会增大反而受制于氧气的扩散速度,氧扩散控制了单晶畴生长。因此,我们认为在pmp-YbCO单晶畴的生长中存在两个生长体系:熔-固体系和气-固体系。在熔-固体系生长中,生长速率R(R和R)与温度过冷度ΔT的二次方成正比;在气-固体系中,生长速率R与气体(氧气)的界面扩散速率成正比。那么就存在两种溶质扩散形式:在熔-固体系中,Y、ba和Cu离子通过熔融液态扩散到达生长界面前沿,其扩散包括溶质扩散和界面扩散两个过程;在气-固体系中,氧气直接从空气中扩散到界面上,溶质扩散仅是界面扩散一个过程。在pmp-YbCO单晶畴的生长中,这两种扩散机制会同时发生作用。YbCO晶畴的生长除了温度过冷度ΔTT外,成分过冷也对单晶畴生长提供了驱动力,所以单晶畴生长的总驱动力来源于温度过冷ΔTT和组分过冷ΔTc之和,ΔT=ΔTT+ΔTc。我们的pmp-YbCO单晶畴块材在77K下测得磁化临界电流密度J为～1.2×10A/cm (77K)。

关键词：高温超导 YbCO单晶畴粉末融化法过冷度生长机制

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