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3D confined self-assembling of QD within super-engineering block copolymers as biocompatible superparticles enabling stimulus responsive solid state fluorescence

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Nano Research 2021年第1期14卷 285-294页

作者： Xiaohong He Kun Jia Robert Marks Yiguo Hu Xiaobo Liu School of Materials and Energy University of Electronic Science and Technology of ChinaChengdu 611731China The avram and stella goldstein-goren Department of Biotechnology Engineering Bert-Gurion University of the NegevBeer-Sheva 8410501Israel State Key Laboratory of Biotherapy Sichuan UniversityChengdu 610051China

Eliminating colloidal toxicity and enabling its intrinsic fluorescence in aggressive environmental conditions are the key challenges for commercializing hydrophobic cadmium based quantum dots(QD).Polyarylene ether nitriles(PEN)are an example of super-engineering thermoplastics that possess a unique combination of thermal stability,intrinsic fluorescence,biocompatibility and distinct emulsion self-assembly ***,the co-self-assembly of amphiphilic PEN with hydrophobic CdSe@ZnS QD,confined in the three-dimensional(3D)oil-in-water emulsion droplets,has been explored tofabricate fluorescent microparticles(FMP).It was found that these FMP demonstrated good biocompatibility(cell viability above 90%),while exhibiting a fluorescence emission in aqueous solution that was retained(intensity retention ratio above 80%)within the whole pH range of 1-14,as well as,after being subjected to autoclaving at 120℃for 1 ***,it was discovered that introduction of calcium ions in the emulsion self-assembly contributed to in-situ generation of phase changing nanoplates inside the FMP,which led to the photo-thermal modulated solid state fluorescence from drop-casted FMP *** to their versatile fluorescence,these FMP colloids were exploited as fluorescent probes for macrophages imaging,while micro-patterns with reversible changing of emission color were induced via thermal treatment and direct laser lithography.

关键词： three-dimensional(3D)confinement self-assembly quantum dots amphiphilic polyarylene ether nitrile stimulus responsive fluorescence

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Antibiofilm activity of 3,3'-diindolylmethane on Staphylococcus aureus and its disinfection on common food-contact surfaces

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Food Science and Human Wellness 2022年第5期11卷 1222-1232页

作者： Hui Zhang Xiaomei Guo Lei Tian Na Wang Yuqing Li Ariel Kushmaro Robert Marks Qun Sun College of Biomass Science and Engineering Sichuan UniversityChengdu 610064China Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education College of Life SciencesSichuan UniversityChengdu 610064China Key Laboratory of Green Chemistry and Technology Ministry of EducationCollege of ChemistrySichuan UniversityChengdu 610064China State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&Department of Cariology and Endodontics West China Hospital of StomatologySichuan UniversityChengdu 610065China avram and stella goldstein-goren Department of Biotechnology Engineering Ben-Gurion University of the NegevBeer-Sheva 84105Israel The Ilse Katz Center for Meso and Nanoscale Science and Technology Ben-Gurion University of the NegevBeer-Sheva 84105Israel

This study explored the antibiofilm efficacy of 3,3’-diindolylmethane(DIM)on Staphylococcus aureus and its disinfection on common food-contact *** minimum biofilm inhibitory concentration(MBIC)of DIM on *** was 62.5μmol/L,while it did not impede the bacterial growth evaluated by growth curve and XTT reduction *** in the concentration range of 31.2-62.5μmol/L demonstrated a dose-dependent antibiofilm activity to ***,as confirmed by light microscopic(LM),confocal laser scanning microscopic(CLSM),and scanning electron microscopic(SEM)*** DIM of62.5μmol/L,the biomass of *** biofilm was significantly reduced by 97%and its average thickness by 58%(P<0.05).DIM of 62.5μmol/L inhibited the bacterial initial adhesion and proliferation,as well as cell motility;the release of extracellular DNA(eDNA)and extracellular polysaccharide(EPS)were reduced by 75%and 69%,*** exhibited a strong inhibition to *** biofilm formation on common food-contact surfaces,including 304 stainless steel,glass,and polyvinyl chloride(PVC)but not disperse the mature ***,our investigation identified DIM as a promising antibiofilm agent and its suitability to prevent the biofilm formation of *** on common food-contact surfaces utilized during food processing.

关键词： Biofilm inhibition 3 3’-Diindolylmethane Extracellular DNA Extracellular polysaccharide Food-contact surfaces

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Modulatory effects of Lactiplantibacillus plantarum on chronic metabolic diseases

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Food Science and Human Wellness 2023年第4期12卷 959-974页

作者： Lei Tian Ruixiang Zhao Xinyi Xu Zhiwei Zhou Xiaofang Xu Dongmei Luo Zhiqiang Zhou Yu Liu Ariel Kushmaro Robert S.Marks András Dinnyés Qun Sun Key Laboratory of Bio-Resource and Eco-Environment of the Ministry of Education College of Life SciencesSichuan UniversityChengdu 610064China Institute for Marine and Antarctic Studies University of TasmaniaNewnham 7248Australia College of Biomass Science and Engineering Sichuan UniversityChengdu 610064China Department of Urology Institute of Urology(Laboratory of Reconstructive Urology)West China HospitalSichuan UniversityChengdu 610041China avram and stella goldstein-goren Department of Biotechnology EngineeringFaculty of Engineering SciencesBen Gurion University of the NegevBeer-Sheva 84105Israel BioTalentum Ltd. Gödöllő2100Hungary Department of Cell Biology and Molecular Medicine University of SzegedSzeged H-6720Hungary Hungarian University of Agriculture and Life Sciences Institute of Physiology and Animal NutritionDepartment of Physiology and Animal HealthGödöllő2100Hungary

The increased global incidence of chronic metabolic diseases,a vital threat to human health and a burden on our healthcare systems,includes a series of clinical metabolic syndromes such as obesity,diabetes,hypertension,and *** of the well-known probiotic microorganisms,Lactiplantibacillus plantarum plays an important role in promoting human health,including inhibiting the occurrence and development of a variety of chronic metabolic *** present study provides an overview of the preventive and therapeutic effects of *** on diabetes,obesity,non-alcoholic fatty liver disease,kidney stone disease,and cardiovascular diseases in animal models and human clinical *** *** demonstrated its ability to reduce inflammatory and oxidative stress levels by regulating the production of cytokines and short-chain fatty acids(SCFAs),the activity of antioxidant enzymes,and the balance of intestinal microbial communities to alleviate the symptoms of chronic metabolic ***,updated applications and technologies of *** in food and biopharmaceutical industries are also *** the characteristics and functions of *** will guide the development of related probiotic products and explore the modulatory benefit of *** supplementations on the prevention and treatment of multiple chronic metabolic diseases.

关键词： Lactiplantibacillus plantarum Diabetes Obesity Non-alcoholic fatty liver disease Kidney stone disease Cardiovascular diseases

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Advances in nano silver-based biomaterials and their biomedical applications

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Engineered Regeneration 2024年第3期5卷 326-341页

作者： Punuri Jayasekhar Babu Akriti Tirkey Abraham Abbey Paul Kathelina Kristollari Jugal Barman Kingshuk Panda Neha Sinha Birudu Ravi Babu Robert S.Marks Biomaterials and Bioengineering Research Laboratory Department of Biotechnology/Life SciencesMizoram UniversityPachhunga University College CampusAizawl 796001MizoramIndia avram and stella goldstein-goren Department of Biotechnology Engineering Ben Gurion University of the NegevBe’er-Sheva 84105Israel Phystech School of Biological and Medical Physics Moscow Institute of Physics and TechnologyMoscow Oblast 141701Russia School of Bio-sciences and Technology Vellore Institute of TechnologyVellore 632014Tamil NaduIndia Department of Physiology/Metabolic Physiology Institute of Neuroscience and PhysiologyFaculty Sahlgrenska AcademyGothenburg UniversityMedicinaregatan 11SE-40530 GöteborgSweden Department of Biotechnology Central Tribal University of Andhra PradeshKonda KarakamAndhra PradeshIndia The Ilse Katz Center for Nanoscale Science and Technology Ben-Gurion University of the NegevBe’er Sheva 84105Israel

Silver nanoparticles are among the most widely researched and used for nanotechnology-derived structures due to their extraordinary inherent optical properties,chemical stability,catalytic activity,and high *** idiosyncratic properties can be attributed to their unique physico-chemical characteristics,such as ultrafine sizes,high surface area,diverse shapes,and strong localized surface plasmon *** distinctive features can be tailored using various physical,chemical,and biological synthesis *** physical techniques are viable for producing silver nanoparticles on a large scale,but they suffer from drawbacks such as high-power con-sumption,expensive set-up,and limited control over nanoparticle size *** methods provide benefits like high yield,consistent shape and size distribution,and cost efficiency,but the residual toxicity of the chemicals involved hinders their biological *** synthesis approaches effectively overcome the limitations of both physical and chemical methods by eliminating the need for hazardous chemicals,requiring less energy,enabling diverse nanoparticle morphologies,and offering eco-friendliness and exceptional *** novel and promising properties of nanosilver-based biomaterials have been demonstrated to be suitable for a wide range of pharmacological and therapeutic biomedical *** extensive application in wound healing,dentistry,cardiovascular disease treatment,nerve tissue engineering,cancer treatment,and biosensing can be attributed to their inherent antimicrobial and antibiofilm activity,antithrombotic properties,potential for nerve regeneration,photothermal conversion efficiency and sensitivity,*** review discusses the different methods employed for synthesising silver nanoparticles and focuses on using nanosilver-based biomaterials for various biomedical applications.

关键词： Nanosilver-biomaterials Dentistry Cardiovascular disease Nerve-tissue engineering Photothermal therapy Biosensing

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