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Chinese Chemical Letters 2004年第1期15卷 7-10页

作者： LiGangLIU YuFangXU XuHongQIAN QingChunHUANG InstituteofPesticides＆pharmaceuticals EastChinaUniversityofScienceandTechnologyShanghai200237 ShanghaiKeyLabofChemicalBiology Shanghai200237 StateKeyLaboratoryofFineChemicals DalianUniversityofTechnologyDalian116012

Novel 1, 3, 4-thia(oxa)diazoles containing thienyl groups were synthesized. Analogous to the naturally-occurring compound, a-terthienyl, they can be photosensitized by ultraviolet light and showed phototoxicities against the 2nd instar larvae of southern armyworm (Pseudaletia separata Walker), they also showed photocleavage action to pBR322 DNA.

关键词： Thiadiazoles oxadiazoles a-terthienyl phototoxic.

Preparation and Selectivity of Molecularly Imprinted Polymer Coating on the Micro Pore Membrane of Polytetrafluoroethylene

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Chinese Chemical Letters 2003年第9期14卷 979-982页

作者： Hong Sheng GUO, Yu Mei JIA, Xi Wen HEState Key Laboratory of Functional Polymer Materials for Adsorption and Separation,Department of Chemistry, Nankai University, Tianjin 300071 The College of pharmaceuticals and Biotechnology, Tianjin University, Tianjin 300072 State Laboratory of Functional Polymer Materials for Adsorption and Separation Department of Chemistry Nankai University The College of pharmaceuticals and Biotechnology Tianjin University

In order to obtain mechanically stable membrane for practical application, the imprinted polymer was synthesized in the pores of polyfluoromembrane, the binding and transport ability of the membrane were studied.

关键词： Molecular imprinting trimethoprim membrane of polytetrafluoroethylene.

Obese diet-induced mouse models of nonalcoholic steatohepatitis-tracking disease by liver biopsy

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World Journal of Hepatology 2016年第16期8卷 673-684页

作者： Maria Nicoline Baandrup Kristiansen Sanne Skovgard Veidal Kristoffer Tobias Gustav Rigbolt Kirstine Sloth Tolbol Jonathan David Roth Jacob Jelsing Niels Vrang Michael Feigh Niels Vrang Michael FeighGubra Aps Intercept pharmaceuticals Inc.

AIM:To characterize development of diet-induced nonalcoholic steatohepatitis(NASH)by performing live biopsy in wild-type and genetically obese ***:Male wild-type C57BL/6J(C57)mice(DIO NASH)and male Lep ob/Lep ob(ob/ob)mice(ob/ob-NASH were maintained on a diet high in trans-fat(40%)fructose(22%)and cholesterol(2%)for 26 and 12 wk respectively.A normal chow diet served as control in C57 mice(lean chow)and ob/ob mice(ob/ob chow)After the diet-induction period,mice were liver biopsied and a blinded histological assessment of steatosis and fibrosis was *** were then stratified into groups counterbalanced for steatosis score and fibrosi stage and continued on diet and to receive daily PO dosing of vehicle for 8 *** gene expression in liver tissue was assessed by RNA sequencing and bioin *** parameters,plasma liver enzyme and lipids(total cholesterol,triglycerides)as well a hepatic lipids and collagen content were measured b biochemical ***-alcoholic fatty liver disease activity score(NAS)(steatosis/inflammation/ballooningdegeneration)and fibrosis were *** and fibrosis were also quantified using percent fractional ***:Diet-induction for 26 and 12 wk in DIONASH and ob/ob-NASH mice,respectively,elicited progressive metabolic perturbations characterized by increased adiposity,total cholesterol and elevated plasma liver *** diet also induced clear histological features of NASH including hepatosteatosis and ***,the metabolic NASH phenotype was more pronounced in ob/ob-NASH vs DIO-NASH *** the eight week repeated vehicle dosing period,the metabolic phenotype was sustained in DIO-NASH and ob/ob-NASH mice in conjunction with hepatomegaly and increased hepatic lipids and collagen *** scoring demonstrated significantly increased NAS of DIO-NASH mice(0 vs4.7±0.4,P<0.001 compared to lean chow)and ob/ob-NASH mice(2.4±0.3 vs 6.3±0.2,P<0.001compared to ob/ob ch

关键词： Nonalcoholic steatohepatitis Liver biopsy Diet-induced obesity Nonalcoholic fatty liver disease Fibrosis

cDNA cloning,sequence analysis,and recombinant expression of akitonin beta,a C-type lectin-like protein from Agkistrodon acutus

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Acta Pharmacologica Sinica 2004年第3期25卷 118-123页

作者： Xiang-dong ZHA1,2,Jing LIU1,Kang-sen XU3 1 School of Life Science,University of Science and Technology,Hefei 230026 School of Life Science,Anhui University,2 Hefei 230039 National Institute for Control of pharmaceuticals and Biological Products,Beijing 100050,China 3 School of Life Science University of Science and Technology School of Life Science Anhui University National Institute for Control of pharmaceuticals and Biological Products Beijing 100050 China

AIM: To clone the cDNA of a new member of snake venom C-type lectin-like proteins, to study its structure- function relationships and to achieve its recombinant production. METHODS: PCR primers were designed based on the homology and cDNA was amplified by RT-PCR using total RNA from snake venom gland as the template. The PCR products were cloned into the plasmid pGEM-T and sequenced. The deduced protein sequence was analyzed with some bioinformatic programs. A recombinant expression plasmid was constructed using pBAD- TOPO as vector and transformed into *** TOP10 competent cells. RESULTS: A novel cDNA sequence encod- ing akitonin β was found and accepted by GenBank (accession number AF387100). Akitonin β consists of a typical carbohydrate recognition domain (CRD) of C-type lectins, and it is homologous with other snake venom C-type lectin-like proteins. It was predicted to be a platelet antagonist. Upon induction with arabinose rAkitonin β express- ing in E coli was achieved at a high level (superior to 150 mg/L). The recombinant fusion protein exhibited inhibitory activities on rat platelet aggregation in vitro. CONCLUSION: A new member of snake venom C-type lectin-like proteins was discovered and characterized, and an efficient recombinant expression system was estab- lished for its production.

关键词： snake venoms cDNA akitonin C-type lectin-like proteins recombinant proteins structure- activity relationship

New meroterpenoids and C-methylated flavonoid isolated from Baeckea frutescens

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Chinese Journal of Natural Medicines 2020年第5期18卷 379-384页

作者： HOU Ji-Qin ZHAO Heng YU Jiang-Hong CHEN Ling-Jun WANG Hao State Key Laboratory of Natural Medicines Department of TCMs PharmaceuticalsSchool of Traditional Chinese PharmacyChina Pharmaceutical UniversityNanjing 210009China

Phytochemical investigation of the aerial parts of Baeckea frutescens resulted in the isolation of three new mono-or sesquiterpene-based meroterpenoids, frutescones S-U(1-3), and one pair of new(±)-5,7-dihydroxy-8-isobutyryl-6-methyldihydroflavonol(4). Their structures and absolute configurations were established by HR-ESI-MS, 1D and 2D NMR, and quantum chemical ECD calculation. Compound 1 exhibited inhibitory effect on NO production in LPS-activated RAW 264.7 macrophages with an IC50 value being 0.81 μmol·L–1.

关键词： Baeckea frutescens Myrtaceae Meroterpenoid Anti-inflammatory

Microfluidic Actuated and Controlled Systems and Application for Lab-on-Chip in Space Life Science

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Space(Science & Technology) 2023年第1期3卷 54-70页

作者： Yimeng Zhao Xuefei Lv Xiaoqiong Li Nino Rcheulishvili Yu Chen Zhe Li Yulin Deng Beijing Key Laboratory for Separation and Analysis in Biomedicine and pharmaceuticals School of Life ScienceBeijing Institute of TechnologyBeijing 100081China

The use of space environment to carry out life science research is of great ***,there are limitations in complex manipulation,research models based on mammalian and 2-dimensional cell culture experiments,*** chips possess the characteristics of integrating the relevant functions of traditional laboratories into micron-scale chips,with low energy consumption,high throughput,and *** application of microfluidic chips can overcome the challenges of space environments,the scarce experimental resources,and the unmanned operation problems to some ***,microfluidic chips can realize long-term experimental operation and remote data recording and *** carry out related research with microfluidic chips,the actuation and control of microfluidics are very *** this paper,the microfluidic actuated and controlled technologies in microfluidic chips are reviewed,and then the application of microfluidic chips and microfluidic actuated and controlled systems in space life science is also briefly described.

关键词： fluid carry overcome

Lethality in mice infected with recombinant vaccinia virus expressing hepatitis C virus core protein

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Hepatobiliary & Pancreatic Diseases International 2003年第3期2卷 374-382页

作者： Hong Zhang the ISIS pharmaceuticals, 2292 Faraday Avenue, Carlsbad, California 92008, USA ISIS pharmaceuticals 2292 Faraday Avenue Carlsbad California 92008 USA. hzhang@***

OBJECTIVE: To establish a mouse model of HCV core expression and investigate the toxicity of HCV core protein or the possible pathogenic effects. METHODS: A series of vaccinia viral expression vectors were engineered to express 5' portion of HCV genes including 5' non-translated region (NTR), core protein, and portion of the E1 gene. These HCV sequences were fused to a luciferase reporter gene and inserted into a vaccinia virus expression vector (pSC11) adjacent to the vaccinia virus promoter, p7.5. The recombinant DNA constructs were packed into infectious recombinant chimeric viruses. The expression of HCV core protein was examined in cultured cells after infection with these viruses. Death of the infected mice was investigated by specific correlation to the expression of HCV core protein and its expression levels. RESULTS: The recombinant virus (VNCE-LUA) expressed HCV core protein and an envelope-luciferase fusion protein in cultured cells. When Balb/c mice were inoculated intraperitoneally with more than 10~7 pfu per mouse of VNCE-LUA, death occurred immediately. The mortality was dependent on the amount of VNCE-LUA virus inoculated. All mice inoculated with 3×10~8 pfu of VNCE-LUA died within 4 days of infection and 50% of mice inoculated with 3×10~7 pfu of VNCE-LUA died within 7 days of infection. No death occurred in mice inoculated with 3×10~8 pfu of a control recombinant vaccinia virus, which expressed luciferase but not the HCV core and envelope proteins. Deletion of core sequences from VNCE-LUA rapidly reduced the mortality of infected mice whereas deletion of envelope sequence did not. SCID mice infected with VNCE-LUA died 2-3 days after infection, suggesting that the HCV-core induced mortality is not dependent on host T-or B-cell responses to core protein. CONCLUSIONS: HCV core protein can be lethal to mice when expressed in vivo and this specific lethality is independent of T-cells or B-cells. The findings and model itself provide a useful tool for fur

关键词： animal model hepatitis C core protein vaccinia virus

Collaborative assembly-mediated siRNA delivery for relieving inflammation-induced insulin resistance

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Nano Research 2020年第11期13卷 2958-2966页

作者： Shiyang Shen Li Zhang Mengru Li Zhizi Feng Huixia Li Xiao Xu Shiqi Lin Ping Li Can Zhang Xiaojun Xu Ran Mo State Key Laboratory of Natural Medicines Jiangsu Key Laboratory of Drug Discovery for Metabolic DiseasesCenter of Advanced Pharmaceuticals and BiomaterialsChina Pharmaceutical UniversityNanjing210009China

Obesity plays a primary causative role in insulin resistance and hyperglycemia that contributes to type 2 *** lipid storage in the liver renders activation of the resident macrophages and chronic secretion of inflammatory mediators,therefore causing or aggravating insulin ***,we develop collaborative assemblies using a“one-pot”synthesis method for macrophage-specific delivery of small interfering RNAs(siRNAs)that target the inflammatory *** nanocomplex(NC)composed of the siRNA molecule,a synthetic thiol-bearing methacrylated hyaluronic acid(sm-HA)and protamine forms through an electrostatic-driven physical assembly,which is chemically crosslinked to acquire the collaboratively assembled nanocapsule(cNC)*** obtained cNC displays significantly higher stability than *** moieties as flexible assembly units can be easily equipped on cNC for long circulation,active targeting,or controlled siRNA ***-F decorated with folic acid,a macrophage-targeting ligand promotes the siRNA accumulation in the activated macrophages in the liver of the obese mouse ***-F loaded with siRNA targeting inflammatory indicators efficiently control the macrophage inflammatory response by reducing the expression of the inflammatory proteins(>40%reduction)and ameliorating the insulin resistance symptoms of the obese mice.

关键词： small interfering RNA(siRNA)delivery collaborative assembly nanomedicine insulin resistance diabetes

Hexagonal packing lattice formed by functionalized gold nanoparticles

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Rare Metals 2022年第11期41卷 3858-3864页

作者： Xiao-Bin Mang Lian-Qi Yao Department of Research and Development Shanghai YS Information Technology Co.LtdShanghai200240China Department of Quality Inspection Shanghai Hutchison PharmaceuticalsShanghai200331China

Nanostructures formed by functionalized gold nanoparticles(GNPs)were studied using X-ray diffraction(XRD).Two systems of such functionalized GNPs,one with gold core grafted by both ligand(rod-like liquid crystalline molecule)and co-ligand(alkylthiols)and the other one with only ligand grafted,were *** presence of ligands and co-ligands increases the mobility of GNPs and induces long-range ordered packing style with the change of *** both systems,functionalized GNPs form columns first and then pack on hexagonal *** is found that the correlation along column direction is dependent on surface conditions of GNPs,which is observed for system with only ligand *** on experimental results,size of gold core and number of ligand and co-ligand belonging to each gold core were *** intensities and electron density map of each structure were simulated,which are evidently similar to experimental ***,structure model was proposed and *** results help us to understand and control the way that organic molecules affect the organization of nanoparticles,and facilitate the design of new hybrid materials.

关键词： Nanostructures Gold nanoparticles Simulation

One-pot Multicomponent Synthesis of Highly Functionalized 1,4-Dihydropyridines Using Porcine Pancreatic Lipase

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Chemical Research in Chinese Universities 2019年第2期35卷 235-238页

作者： JIANG Ling YE Wenting SU Weike Collaborative Innovation Center of Yangtze River Delta Region Green pharmaceuticals Zhejiang University of TechnologyHangzhou 310014P.R.China

A series of highly timctionalized 1,4-dihydropyridines was synthesized via one-pot multicomponent reactions of aromatic aldehyde,malononitrile and N-methyl-1-(methylthio)-2-nitroethenamine using Porcine pancreatic lipase(PPL)as catalyst in *** protocol is featured by mild reaction conditions,simple operation and environmental acceptability.

关键词： Porcine pancreatic lipase Biocatalysis One-pot synthesis High functionalized 1,4-dihydropyridines