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Influence of kavain on hepatic ultrastructure

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World Journal of Gastroenterology 2008年第4期14卷 541-546页

作者： Shuang Fu Emine Korkmaz Filip Braet Quan Ngo Iqbal Ramzan Faculty of Pharmacy The Universityof Sydney NSW 2006 Australia australian key centre formicroscopy and microanalysis Electron Microscopy Unit TheUniversity of Sydney NSW 2006 Australia Faculty of Medicine The University of Sydney NSW2006 Australia

AIM: To investigate whether the major kavalactone kavain imposes adverse effects on the liver ultrastructure and function by affecting vascular and microvascular architecture and altering hepatocellular morphology. METHODS: Kavain solution (10 μg/mL or 43.5 μmol/L) was perfused for 2 h in isolated rat livers. After standard fi xation and tissue preparation,the samples were examined by scanning electron microscopy (SEM),transmission electron microscopy (TEM),and light microscopy (LM). RESULTS: LM,SEM,and TEM examinations indicated kavain-treated rat livers (n = 4) displayed severe vascular and endothelial damage compared to control livers (n = 4). CONCLUSION: The data so far support the hypothesis that kavain induces adverse effects on liver; additional investigations with other kavalactones and their effects on liver are urgently needed.

关键词： Kava Kavalactones Kavain Liver Electronmicroscopy

Unlocking the ultrastructure of colorectal cancer cells in vitro using selective staining

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World Journal of Gastroenterology 2010年第22期16卷 2743-2753页

作者： Joanna M Biazik Kristina A Jahn Filip Braet australian key centre for Microscopy and microanalysis Madsen Building F09 The University of Sydney

AIM: To characterise differences between three widely used colorectal cancer cell lines using ultrastructural selective staining for glycogen to determine variation in metastatic properties. METHODS: Transmission electron microscopy was used in this investigation to help identify intracellular structures and morphological features which are precursors of tumor invasion. In addition to morphological markers, we used selective staining of glycogen as a marker for neoplastic cellular proliferation and determined whether levels of glycogen change between the three different cell lines. RESULTS: Ultrastructural analysis revealed morphological differences between the cell lines, as well as differentiation into two sub-populations within each cell line. Caco-2 cells contained large glycogen deposits as well as showing the most obvious morphological changes between the two sub-populations. SW480 cells also contained large glycogen stores as well as deep cellular protrusions when grown on porous filter membranes. HT-29 cells had trace amounts of glycogen stores with few cellular projections into the filter pores and no tight junction ***: Morphology indicative of metastatic properties coincided with larger glycogen deposits, providing strong evidence for the use of selective staining to determine the neoplastic properties of cells.

关键词： Cancer Colorectal Electron microscopy Glycogen Potassium ferrocyanide

Effect of Small Addition of Sc on the Microstructural Evolution in Al-15Ag Alloy

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Journal of Materials Science & Technology 2005年第5期21卷 630-634页

作者： Zhiguo CHEN Ziqiao ZHENG Simon P.Ringer School of Materials Science and Engineering Central South University Changsha 410083 China australian key centre for Microscopy & microanalysis The University of Sydney 2006 NSW Australia

The effect of small addition of Sc on the microstructural evolution in Al-15Ag alloy was investigated by Monte Carlo simulation and transmission electron microscopy （TEM）. Monte Carlo simulation results indicate that Ag clustering is suppressed by the addition of Sc during the initial ageing stage, and there is a little chemical repulsion between Ag atoms and Sc atoms. TEM observations show that the morphology of γ′ precipitation can be modified by the small addition of Sc, which does result in a refinement of γ′ precipitates in Al-15Ag-0.2Sc. The mechanism by which trace Sc affect the microstructure is to reduce the ledge density of γ′precipitates.

关键词： Aluminium alloys Ageing Microstructure Scandium

Effect of Thermo-Mechanical Processing on Precipitate Formation in Next Generation HSLA Steel

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Journal of Iron and Steel Research International 2011年第S1期18卷 478-481页

作者： Mukherjee S Timokhina I B Ringer S P Hodgson P D centre for Material and Fibre Innovation Deakin UniversityGeelongVic 3217Australia australian key centre for Microscopy and microanalysis University of SydneyNSWAustralia

Development of advanced high strength steels (AHSS) using a conventional rolling setup is one of the biggest challenges to steel *** has been found that fine precipitation in a soft matrix,formed after hot rolling,can markedly improve the mechanical *** this work,three dimensional atom probe tomography (3D-APT) has been used to study the formation of precipitates in thermomechanically simulated steel.3D-APT data reveals co-existence of numerous nano clusters with ***,quantitative analysis of the nano clusters and precipitates shows clusters are as small as 1nm in *** are found to be disc shaped with the composition of equilibrium precipitates (TiMo)***,3D-APT is seen as an ideal technique to complement TEM to understand the nanoscale features in thermomechanically processed steel for further improvements in the mechanical properties of AHSS.

关键词： TMCP 3D-APT precipitate cluster

Structural and functional aspects of the liver and liver sinusoidal cells in relation to colon carcinoma metastasis

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World Journal of Gastroenterology 2005年第33期11卷 5095-5102页

作者： Katrien Vekemans Filip Braet centre of Experimental Surgery and Anaesthesia Abdominal Transplant Surgery Department Catholic University of Leuven (KUL)Herestraat 49 3000 Leuven Belgium australian key centre for Microscopy and microanalysis Electron Microscopy Unit The University of SydneySydney 2006NSWAustralia

Nowadays, liver metastasis remains difficult to cure. When tumor cells escape and arrive in the liver sinusoids, they encounter the local defense mechanism specific to the liver. The sinusoidal cells have been widely described in physiologic conditions and in relation to metastasis during the past 30 years. This paper provides an “overview” of how these cells function in health and in diseases such as

关键词： Metastasis Liver Colon carcinoma Sinusoidalcells Kupffer cells Liver sinusoidal endothelial cells Hepatic NK cells

微量钪对Al-2.5Cu-1.5Mg合金时效特性与微观组织的影响

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稀有金属材料与工程 2004年第8期33卷 811-814页

作者：陈志国郑子樵 Ringer S P 中南大学湖南长沙410083 australian key centre for Microscopy & microanalysis The University of Sydney NSW 2006 Australia

研究了微量钪对Al-2.5Cu-1.5Mg合金微观组织与时效特性的影响。结果表明,微量钪的添加增强了Al-2.5Cu-1.5Mg合金的时效硬化效果,但并未改变该合金时效硬化的总体规律。透射电镜分析发现GPB在合金时效硬化第二阶段初期才开始析出,并且是... 详细信息

研究了微量钪对Al-2.5Cu-1.5Mg合金微观组织与时效特性的影响。结果表明,微量钪的添加增强了Al-2.5Cu-1.5Mg合金的时效硬化效果,但并未改变该合金时效硬化的总体规律。透射电镜分析发现GPB在合金时效硬化第二阶段初期才开始析出,并且是该合金第二阶段硬化的主要原因,微量钪的添加促进了Al-2.5Cu-1.5Mg合金中GPB的弥散细小析出。

关键词：铝合金时效显微组织钪

微量钪对Al-15Ag合金时效特性与微观组织的影响

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稀有金属材料与工程 2004年第9期33卷 928-932页

作者：陈志国郑子樵 Simon P.Ringer 中南大学湖南长沙410083 australian key centre for Microscopy and microanalysis the University of Sydney NSW 2006Australia

通过时效硬化曲线测量及时效组织分析,研究了微量钪对Al-15Ag合金时效行为和显微组织的影响。在Al-15Ag合金中添加0.2%Sc(质量分数,下同),可以增强合金在190℃和350℃时效的时效硬化效果,延长峰时效的到达时间。微量钪的添加促使合金中... 详细信息

通过时效硬化曲线测量及时效组织分析,研究了微量钪对Al-15Ag合金时效行为和显微组织的影响。在Al-15Ag合金中添加0.2%Sc(质量分数,下同),可以增强合金在190℃和350℃时效的时效硬化效果,延长峰时效的到达时间。微量钪的添加促使合金中γ′相细小密集地析出,同时钪的存在减少了γ′相宽面上的位错台阶数。含钪Al-15Ag合金中γ′相长大过程比较缓慢的微观机理是微量钪的添加影响了合金中γ′相宽面上的台阶分布。

关键词：铝合金时效显微组织钪

Stable tin perovskite solar cells developed via additive engineering

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Science China Materials 2021年第11期64卷 2645-2654页

作者： Zhensheng Dai Taoyuze Lv Julien Barbaud Wentao Tang Tao Wang Liang Qiao Han Chen Rongkun Zheng Xudong Yang Liyuan Han State key Laboratory of Metal Matrix Composites School of Materials Science and EngineeringShanghai Jiao Tong UniversityShanghai 200240China Joint Research Center for Clean Energy Materials Shanghai Jiao Tong UniversityShanghai 200240China School of Physics Australian Centre for Microscopy&MicroanalysisSydney Nano InstituteThe University of SydneySydneyNSW 2006Australia

Tin perovskite solar cells(TPSCs)are promising for lead-free perovskite solar cells(PSCs)and have led to extensive research;however,the poor crystallinity and chemical stability of tin perovskites are two issues that prevent stable *** this study,we outline a new process that addresses these issues by using tin(II)acetate(Sn(Ac)2)in place of the conventional SnF2 precursor *** with SnF2,Sn(Ac)2 improves the crystallinity and stability of tin perovskite with fewer defects and better charge *** this process,we developed a device that has a higher external quantum efficiency for charge extraction compared with the control devices and a power conversion efficiency of 9.93%,which maintained more than 90%of its initial efficiency after 1000 h operation at the maximum power point under standard AM 1.5G solar illumination.

关键词： perovskite solar cells stability lead-free perovskite tin(II)acetate FASnI3

Stable tin perovskite solar cells enabled by widening the time window for crystallization

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Science China Materials 2021年第8期64卷 1849-1857页

作者： Zhensheng Dai Wentao Tang Tao Wang Taoyuze Lv Xinhui Luo Danyu Cui Ruitian Sun Liang Qiao Han Chen Rongkun Zheng Xudong Yang Liyuan Han State key Laboratory of Metal Matrix Composites School of Material Science and EngineeringShanghai Jiao Tong UniversityShanghai 200240China Joint Research Center for Clean Energy Materials Shanghai Jiao Tong UniversityShanghai 200240China School of Physics Australian Centre for Microscopy&MicroanalysisSydney Nano InstituteThe University of SydneySydneyNSW 2006Australia

Tin perovskite solar cells (TPSCs) are the most promising candidates for lead-free perovskite solar cells(PSCs).However,the poor crystallization and chemical stability of Sn perovskites are the two challenging issues for further application of ***,we present a strategy to stabilize CH(NH_(2))2SnI3(FASnI3) perovskite enabled by an amine complex,CH3NH3I·3CH3NH_(2),which can hinder the major degradation issue caused by the oxidation of Sn2+to Sn4+.The resulting Sn perovskite films exhibit enhanced crystallinity and stability in comparison with those made with conventional inorganic SnF2 ***,the device achieved a higher external quantum efficiency for charge extraction and a power conversion efficiency (PCE) of 9.53%,which maintained more than 90%of the initial efficiency after1000 h of light soaking under the standard AM 1.5 G solar illumination.

关键词： lead-free perovskite FASnI3 perovskite solar cells crystallization stability