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Unlocking the ultrastructure of colorectal cancer cells in vitro using selective staining

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World Journal of Gastroenterology 2010年第22期16卷 2743-2753页

作者： Joanna M Biazik Kristina A Jahn Filip Braet australian Key centre for microscopy and microanalysis Madsen Building F09 The University of Sydney

AIM: To characterise differences between three widely used colorectal cancer cell lines using ultrastructural selective staining for glycogen to determine variation in metastatic properties. METHODS: Transmission electron microscopy was used in this investigation to help identify intracellular structures and morphological features which are precursors of tumor invasion. In addition to morphological markers, we used selective staining of glycogen as a marker for neoplastic cellular proliferation and determined whether levels of glycogen change between the three different cell lines. RESULTS: Ultrastructural analysis revealed morphological differences between the cell lines, as well as differentiation into two sub-populations within each cell line. Caco-2 cells contained large glycogen deposits as well as showing the most obvious morphological changes between the two sub-populations. SW480 cells also contained large glycogen stores as well as deep cellular protrusions when grown on porous filter membranes. HT-29 cells had trace amounts of glycogen stores with few cellular projections into the filter pores and no tight junction ***: Morphology indicative of metastatic properties coincided with larger glycogen deposits, providing strong evidence for the use of selective staining to determine the neoplastic properties of cells.

关键词： Cancer Colorectal Electron microscopy Glycogen Potassium ferrocyanide

On the microstructure and texture evolution in 17-4 PH stainless steel during laser powder bed fusion: Towards textural design

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Journal of Materials Science & Technology 2022年第22期117卷 183-195页

作者： M.S.Moyle N.Haghdadi X.Z.Liao S.P.Ringer S.Primig School of Materials Science&Engineering UNSW SydneyNSW 2052Australia australian centre for microscopyµanalysis The University of SydneyNSW 2006Australia School of Aerospace Mechanical and Mechatronic EngineeringThe University of SydneyNSW 2006Australia

Additive Manufacturing (AM) of metals allows the production of parts with complex designs, offeringadvanced properties if the evolution of the texture can be controlled. 17-4 precipitation hardening (PH)stainless steel is a high strength, high corrosion resistance alloy used in a range of industries suitable forAM, such as aerospace and marine. Despite 17-4 PH being one of the most common steels for AM, thereare still gaps in the understanding of its AM processing–structure relationships. These include the natureof the matrix phase, as well as the development of texture through AM builds under different processingconditions. We have investigated how changing the laser power and scanning strategy affects the microstructure of 17-4 PH during laser powder bed fusion. It is revealed that the matrix phase is δ-ferritewith a limited austenite presence, mainly in regions of the microstructure immediately below melt *** fraction is independent of the printing pattern and laser power. However, reducing the timebetween adjacent laser passes during printing results in an increase in the austenite volume *** effect of the higher laser power, as well as additional remelting within the printing strategy, isan increase in the average grain size by epitaxial ferrite grain growth across multiple build layers andthe development of a mosaic type microstructure. Changes to the scanning strategy have significant impacts on the textures observed along the build direction, while (100) texture along the scanning directionis observed consistently. Mechanisms for texture formation and the mosaic structure are proposed thatpresents a pathway to the design of texture via AM process control.

关键词： Additive manufacturing 17-4 PH stainless steel Microstructure Texture

Tribological behaviour of fused deposition modelling printed short carbon fibre reinforced nylon composites with surface textures under dry and water lubricated conditions

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Friction 2022年第12期10卷 2045-2058页

作者： Ming LUO Siyu HUANG Ziyan MAN Julie M.CAIRNEY Li CHANG School of Aerospace Mechanical and Mechatronic EngineeringThe University of SydneySydney NSW 2006Australia australian centre for microscopy and microanalysis The University of SydneySydney NSW 2006Australia

Fused deposition modelling(FDM)printed short carbon fibre reinforced nylon(SCFRN)composites were *** friction and wear behaviour of printed materials were systematically investigated under both dry sliding and water lubricated *** results showed that with short fibre enhancements,the printed SCFRN achieved a lower friction coefficient and higher wear resistance than nylon under all tested ***,under water lubricated conditions,the printed SCFRN exhibited a low,stable friction coefficient due to the cooling and lubricating effects of ***,the specific wear rate of the printed specimens could be higher than that obtained under dry sliding conditions,especially when the load was relatively *** square textured surface was designed and created in the printing process to improve materials’tribological *** was found that with the textured surface,the wear resistance of the printed SCFRN was improved under dry sliding conditions,which could be explained by the debris collection or cleaning effect of surface ***,such a cleaning effect was less noticeable under lubricated conditions,as the liquid could clean the surface *** the other hand,surface textures could increase the surface area exposed to water,causing surface softening due to the higher water absorption *** a result,the samples having surface textures showed higher wear rates under lubricated *** work has provided new insights into designing wear resistant polymer materials using three-dimensional(3D)printing technologies,subjected to different sliding conditions.

关键词： fused deposition modelling(FDM) short carbon fibre reinforced nylon(SCFRN)composites transfer film friction and wear

5083铝合金中θ-Al_(45)(Mn,Cr)_7相的孪晶现象

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中国有色金属学报 2019年第4期29卷 684-692页

作者：肖晓玲刘宏伟陈文龙詹浩孙红英广东省工业分析检测中心广州510650 australian centre for microscopyµanalysis The University of Sydney

应用高分辨透射电镜观察了5083铝合金轧制退火组织中弥散相的形态和微观结构。结果表明:呈球状或不规则形状、尺寸约200～300 nm的弥散相为θ-Al_(45)(Mn,Cr)_7相,属单斜结构,晶胞大小a=2.5196 nm,b=0.7574nm,c=1.0949 nm,β=128.72°... 详细信息

应用高分辨透射电镜观察了5083铝合金轧制退火组织中弥散相的形态和微观结构。结果表明:呈球状或不规则形状、尺寸约200～300 nm的弥散相为θ-Al_(45)(Mn,Cr)_7相,属单斜结构,晶胞大小a=2.5196 nm,b=0.7574nm,c=1.0949 nm,β=128.72°。另外,还发现该相颗粒内部易产生孪晶、孪晶畴和复合孪晶,伴有二次孪晶(或微孪晶)和反相畴界等晶体缺陷,孪晶面分别为(111)或(111),共轭面为(110)或(110)。同时从晶体学角度解释了该相孪晶和复合孪晶等缺陷的生成机制。

关键词： 5083铝合金透射电镜 θ-Al45(Mn,Cr)7相孪晶

Effect of Small Addition of Sc on the Microstructural Evolution in Al-15Ag Alloy

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Journal of Materials Science & Technology 2005年第5期21卷 630-634页

作者： Zhiguo CHEN Ziqiao ZHENG Simon P.Ringer School of Materials Science and Engineering Central South University Changsha 410083 China australian Key centre for microscopy & microanalysis The University of Sydney 2006 NSW Australia

The effect of small addition of Sc on the microstructural evolution in Al-15Ag alloy was investigated by Monte Carlo simulation and transmission electron microscopy （TEM）. Monte Carlo simulation results indicate that Ag clustering is suppressed by the addition of Sc during the initial ageing stage, and there is a little chemical repulsion between Ag atoms and Sc atoms. TEM observations show that the morphology of γ′ precipitation can be modified by the small addition of Sc, which does result in a refinement of γ′ precipitates in Al-15Ag-0.2Sc. The mechanism by which trace Sc affect the microstructure is to reduce the ledge density of γ′precipitates.

关键词： Aluminium alloys Ageing Microstructure Scandium

Effect of Thermo-Mechanical Processing on Precipitate Formation in Next Generation HSLA Steel

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Journal of Iron and Steel Research International 2011年第S1期18卷 478-481页

作者： Mukherjee S Timokhina I B Ringer S P Hodgson P D centre for Material and Fibre Innovation Deakin UniversityGeelongVic 3217Australia australian Key centre for microscopy and microanalysis University of SydneyNSWAustralia

Development of advanced high strength steels (AHSS) using a conventional rolling setup is one of the biggest challenges to steel *** has been found that fine precipitation in a soft matrix,formed after hot rolling,can markedly improve the mechanical *** this work,three dimensional atom probe tomography (3D-APT) has been used to study the formation of precipitates in thermomechanically simulated steel.3D-APT data reveals co-existence of numerous nano clusters with ***,quantitative analysis of the nano clusters and precipitates shows clusters are as small as 1nm in *** are found to be disc shaped with the composition of equilibrium precipitates (TiMo)***,3D-APT is seen as an ideal technique to complement TEM to understand the nanoscale features in thermomechanically processed steel for further improvements in the mechanical properties of AHSS.

关键词： TMCP 3D-APT precipitate cluster

Structural and functional aspects of the liver and liver sinusoidal cells in relation to colon carcinoma metastasis

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World Journal of Gastroenterology 2005年第33期11卷 5095-5102页

作者： Katrien Vekemans Filip Braet centre of Experimental Surgery and Anaesthesia Abdominal Transplant Surgery Department Catholic University of Leuven (KUL)Herestraat 49 3000 Leuven Belgium australian Key centre for microscopy and microanalysis Electron microscopy Unit The University of SydneySydney 2006NSWAustralia

Nowadays, liver metastasis remains difficult to cure. When tumor cells escape and arrive in the liver sinusoids, they encounter the local defense mechanism specific to the liver. The sinusoidal cells have been widely described in physiologic conditions and in relation to metastasis during the past 30 years. This paper provides an “overview” of how these cells function in health and in diseases such as

关键词： Metastasis Liver Colon carcinoma Sinusoidalcells Kupffer cells Liver sinusoidal endothelial cells Hepatic NK cells

Crystallization behavior of Ni_(54)Mn_(25.1)Ga_(20.9) ferromagnetic shape memory thin film

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Rare Metals 2007年第S1期26卷 139-142页

作者： MARTIN Saunders centre for microscopy and microanalysis University of Western AustraliaNedlandsW.A.6009Australia

NiMnGaferromagnetic shape memory thin film was deposited onto Al foil using *** sputtering *** crystallization behavior of the film was investigated by XRD and *** activation energy of crystallization of the film was calculated by Kissinger’s *** results show that the crystallization temperature of NiMnGafree-standing thin film in martensite state is 372 ℃,and the activation energy of crystallization is about 191.9 kJ·mol-1.

关键词： Ni-Mn-Ga ferromagnetic shape memory alloys thin films crystallization

Compositional design and microstructure analysis of Zr-based bulk metallic glasses

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中国有色金属学会会刊：英文版 2007年第6期17卷 1433-1440页

作者：严明邹进沈军孙剑飞 School of Materials Science and Engineering Harbin Institute of Technology School of Engineering and centre for microscopy and microanalysis University of Queensland QLD 4072 Australia

The systematical studies of Zr-based BMGs were summarized in terms of their compositional design and their structural characterization. In particular, several key issues of BMG materials were focused, including initial alloy design and subsequent composition optimization, solidification microstructure characterization and crystallization process specification. The results show that a compositional designing approach is successfully developed and, through extensive microstructure characterization using transmission electron microscopy, several new crystalline phases are discovered in these newly developed Zr-based BMG alloys. Crystallization behavior of Zr-based BMG is also determined based on the microstructure analysis.

关键词：金属玻璃微观结构凝固结晶