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Altered microRNA composition in the uterine lumen fluid in cattle(Bos taurus)pregnancies initiated by artificial insemination or transfer of an in vitro produced embryo

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Journal of Animal Science and Biotechnology 2025年第1期16卷 76-91页

作者： Fernando H.Biase Sarah E.Moorey Julie G.Schnuelle Soren Rodning Martha Sofia Ortega Thomas E.Spencer School of Animal Sciences Virginia Polytechnic Institute and State University175 W Campus DrBlacksburgVA 24061USA Department of Animal Science University of TennesseeKnoxvilleTN 37996USA Department of Clinical Sciences Auburn UniversityAuburnAL 36849USA Department of Animal Science Auburn UniversityAuburnAL 36849USA Department of Animal and Dairy Sciences University of Wisconsin MadisonMadisonWI 53706USA Division of Animal Sciences University of MissouriColumbiaMO 65211USA

Background MicroRNAs(miRNAs)are presented in the uterine lumen of many mammals,and in vitro experiments have determined that several miRNAs are important for the regulation of endometrial and trophoblast *** aim was to identify and contrast the miRNAs present in extracellular vesicles(EVs)in the uterine lumen fluid(ULF)at the onset of attachment in cattle pregnancies(gestation d 18)initiated by artificial insemination(AI)or by the trans-fer of an in vitro-produced blastocyst(IVP-ET).A third group had no conceptus after the transfer of an IVP *** The abundance of 263 annotated miRNAs was quantified in the EVs collected from *** was an increase in the transcript abundance of 20 miRNAs in the ULF EVs from the AI pregnant group,while 4 miRNAs had a lower abundance relative to the group not containing a ***,4 miRNAs were more abun-dant in ULF EVs in the AI pregnant group relative to IVP-ET group(bta-mir-17,bta-mir-7-3,MIR7-1,MIR18A).Specific miRNAs in the ULF EVs were co-expressed with messenger RNAs expressed in extra-embryonic tissues and endome-trium,including genes that are known to be their *** The results provide biological insights into the participation of miRNAs in the regulation of trophoblast proliferation and differentiation,as well as in endometrium *** knowledge that in vitro cultured embryos can contribute to the altered abundance of specific miRNAs in the uterine lumen can lead to the development of cor-rective approaches to reduce conceptus losses during the first month of pregnancy in cattle.

关键词： Artificial reproductive technology Cattle Conceptus Embryos Endometrium Small RNA

Environmental colloid behaviors of humic acid-Cadmium nanoparticles in aquatic environments

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Journal of Environmental Sciences 2025年第3期149卷 663-675页

作者： Ruyi Zheng Jian Zhu Peng Liao Dengjun Wang PanWu Wenjian Mao Yuqin Zhang Weiwei Wang College of Resources and Environment Engineering Key Laboratory of Karst Georesources and EnvironmentMinistry of EducationGuizhou UniversityGuiyang 550025China Guizhou Karst Environmental Ecosystems Observation and Research Station Ministry of EducationGuizhou UniversityGuiyang 550025China State Key Laboratory of Environmental Geochemistry Institute of GeochemistryChinese Academy of ScienceGuiyang 550081China School of Fisheries Aquaculture and Aquatic SciencesAuburn UniversityAuburnAL 36849USA

Humic acid(HA),a principal constituent of natural organic matter(NOM),manifests ubiquitously across diverse ecosystems and can significantly influence the environmental behaviors of Cd(II)in aquatic *** studies on NOM-Cd(II)interactions have primarily focused on the immobilization of Cd(II)solids,but little is known about the colloidal stability of organically complexed Cd(II)particles in the *** this study,we investigated the formation of HA-Cd(II)colloids and quantified their aggregation,stability,and transport behaviors in a saturated porous media representative of typical subsurface *** from batch experiments indicated that the relative quantity of HA-Cd(II)colloids increased with increasing C/Cd molar ratio and that the carboxyl functional groups of HA dominated the stability of HA-Cd(II)*** results of correlation analysis between particle size,critical aggregation concentration(CCC),and zeta potential indicated that both Derjaguin-Landau-Verwey-Overbeek(DLVO)and non-DLVO interactions contributed to the enhanced colloidal stability of HA-Cd(II)*** results further confirmed that the stable HA-Cd(II)colloid can transport fast in a saturated media composed of clean ***,this study provides newknowledge of the colloidal behaviors of NOM-Cd(II)nanoparticles,which is important for better understanding the ultimate cycling of Cd(II)in aquatic systems.

关键词： HA-Cd(II)colloids Colloid formation Colloid aggregation Transport

Late spring-early summer drought and soil properties jointly modulate two pine species? decline and climatic sensitivity in temperate Northern China

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Forest Ecosystems 2025年第01期 146-158页

作者： Yuheng Li Zhaofei Fan Lihong Xu Xiao Zhang Zhongjie Shi Xiaohui Yang Wei Xiong Ling Cong Semyun Kwon Leilei Pan Hanzhi Li Shuo Wen Xiaoyan Shang Institute of Geographic Sciences and Natural Resources Research Chinese Academy of Sciences Research Institute of Ecological Conservation and Restoration Chinese Academy of Forestry university of Chinese Academy of Sciences College of Forestry Wildlife and Environment Auburn University Ecology and Nature Conservation Institute Chinese Academy of Forestry Research Institute of Desertification Studies Chinese Academy of Forestry Institute of Ecological Restoration Kongju National University Chungcheongnam-do

Increasing temperatures and severe droughts threaten forest vitality globally. Prediction of forest response to climate change requires knowledge of the spatiotemporal patterns of monthly or seasonal climatic impacts on the growth of tree species, likely driven by local climatic aridity, climate trends, edaphic conditions, and the climatic adaption of tree species. The ability of tree species to cope with changing climate and the effects of environmental variables on growth trends and growth-climate relationships across diverse bioclimatic regions are still poorly understood for many species. This study investigated radial growth trends, interannual growth variability, and growth-climate sensitivity of two dominant tree species, Pinus tabulaeformis(PT) and Pinus sylvestris var. mongolica(PS), across a broad climatic gradient with a variety of soil properties in temperate Northern China. Using a network of 83 tree ring chronologies(54 for PT and 29 for PS) from 1971 to 2010, we documented that both species maintained constant growth trends at wet sites, while both displayed rapid declines at dry sites. We reported the species-specific drivers of spatial heterogeneity in growth trends, interannual growth variability, and growth-climate relationships. Calculated climatic variables and soil properties were identified as the most critical factors affecting the growth trends and growth-climate relationships. However, climatic variables play more essential roles than soil properties in determining the spatial heterogeneity of the growth-climate *** clay content and higher soil nutrient regimes can exacerbate the moisture-related susceptibility of tree growth. Our findings highlight that soil properties emerged as important modulating factors to predict the drought vulnerability of forests in addition to climatic variables. Considering the continued climate warmingdrying trend in the future, both pines will face a more severe growth decline and increase in dr

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The Demographic Impact on Credit Scores＂ Evidence From Statistical Methods and Geographic Information Systems （GIS） Mapping

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Journal of Modern Accounting and Auditing 2013年第11期9卷 1497-1506页

作者： Anna E. Newman Joseph A. Newman auburn university montgomery Montgomery USA

Average credit scores for people in the United States （US） differ from state to state. Some states have high, and some states have low average credit scores. Since lenders and employers use credit scores to make loan and employment decisions, people living in states where average credit scores are high should experience the benefits of living where credit scores tend to allow more favorable loan and employment decisions. Although credit scores are the direct result of credit histories, credit histories may be impacted by demographic factors. If the demographic factors that impact credit histories are identified, ways to improve credit scores are likely to be discovered and available to people and state government policymakers. This study looks for demographic factors to indirectly explain the average credit scores for people living in each state of the US. The methodology includes statistical analyses and geographic information systems （GIS） mapping. Statistical analyses provide evidence to suggest that state average credit scores are explained by the demographic factors of education, family, income, and health. GIS mapping reveals clusters of states with similar demographics and credit scores.

关键词： credit scores demographics geographic information systems (GIS) mapping

The influence of various grease compositions and silver nanoparticle additives on electrically induced rolling-element bearing damage

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Friction 2024年第4期12卷 796-811页

作者： Samuel BOND Robert L.JACKSON German MILLS Department of Chemistry and Biochemistry Auburn UniversityAuburn 36849-5412USA Department of Mechanical Engineering Auburn UniversityAuburn 36849-5412USA

Leakage currents accelerate surface degradation of metal contacts via small scale arcing across lubricating films,but recent observations suggest that metallic nanoparticle additives in lubricants may be useful to improve contact *** findings prompted a study that examined electrically induced surface pitting of steel contacts in the presence of several lubricating greases including some containing nanometer-sized colloidal silver(Ag)*** rolling sphere-on-disk experiments were conducted under electro-tribological loads employing polyurea greases derived from mineral and synthetic base oils with and without *** forces and electrical resistance were monitored continuously during the tests;surface changes were characterized by means of optical spectroscopy,stylus profilometry,and scanning electron microscopy(SEM)including compositional analysis using energy dispersive spectroscopy(EDS).The observations demonstrate that surface pitting induced by arcing occurs mainly at the points were the rolling motion changes direction and that eroded metal is deposited along the wear ***-sized pits are formed which contain carbon and oxygen indicating that arcing causes decomposition of the hydrocarbon *** findings indicate a significant inhibition of pitting is induced by the Ag nanoparticles;some greases containing other additives exhibit a similar,although less pronounced,effect.

关键词： electric motor bearings wheel bearings electric vehicles arcing electrically induced bearing damage pitting leakage current stray current

Experimental Analysis of Atypically Long Finned Oscillating Heat Pipe for Ventilation Waste Heat Recovery Application

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Journal of Thermal Science 2020年第3期29卷 667-675页

作者： MAHAJAN Govinda CHO Heejin SMITH Aaron THOMPSON Scott M. Rheem Manufacturing MontgomeryAL 36109USA Department of Mechanical Engineering Mississippi State UniversityMsissippi StateMS 39762USA Department of Mechanical Engineering Auburn UniversityAubumnAL 36849USA

Oscillating heat pipes(OHP)which are constructed from a serpentine-arranged capillary tube possess a desirable aerodynamic form factor and provide for relatively high heat transfer rates via cyclic evaporation and condensation of an encapsulated working fluid with no internal wicking structure *** last two decades,OHP has been extensively investigated for its potential application in thermal management of various *** study presents an experimental investigation on the heat transfer performance of an atypically long finned *** heat transfer performance of the proposed OHP was analyzed and compared with a bare tube OHP with similar overall *** show that a unit row of finned OHP filled with n-pentane with fill ratio of 70%can recover up to(400±40)W of heat from a typical waste exhaust air *** additional pressure drop due to fins was estimated to be(6.8±2)Pa resulting in an increase of 1–2 W of fan power *** average heat recovery rate via finned OHP was found to be almost 80%more than bare tube OHP filled with same working fluid with same fill ratio.

关键词： oscillating heat pipe waste heat recovery ventilation

Natural nest substrates influence squamate embryo physiology but have little effect on hatchling phenotypes

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Integrative Zoology 2022年第4期17卷 550-566页

作者： Joshua M.HALL Jocelyn MIRACLE Cindy D.SCRUGGS Daniel A.WARNER Department of Biological Sciences Auburn UniversityAuburnAlabamaUSA

Vertebrate embryos require access to water;however,many species nest in terrestrial habitats that vary considerably in moisture ***,non-avian reptiles have served as models to understand how environmental factors,like moisture availability,influence development because eggs are often exposed to prevailing environments in the absence of parental *** much research demonstrates the importance of water absorption by eggs,many ecological factors that influence moisture availability in natural nests have received little *** example,the type of substrate in which nests are constructed is *** experimentally incubated eggs of the brown anole lizard(Anolis sagrei)in 2 naturally occurring nest substrates that were treated with varying amounts of water to determine how natural substrates influence development at different moisture *** substrate consisted of sand and crushed seashells and the other was mostly organic material(*** plant material).Both are common nesting substrates at our field *** controlling for water uptake by eggs,we found that egg survival and hatchling phenotypes were similar between substrates;however,embryos developed more quickly in the sand/shell substrate than the organic substrate,indicating substrate-specific effects on embryo *** results demonstrate that different natural substrates can result in similar developmental outcomes if the water available to eggs is comparable;however,some aspects of development,like developmental rate,are affected by the type of substrate,independent of water *** study is required to determine how natural substrates influence embryo physiology independent of water content.

关键词： developmental plasticity egg incubation hydric conditions nesting behavior nest-site selection

Semi-Interpenetrating Novolac-Epoxy Thermoset Polymer Networks Derived from Plant Biomass

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Journal of Renewable Materials 2018年第7期6卷 724-736页

作者： Mehul Barde Yusuf Celikbag Brian Via Sushil Adhikari Maria LAuad Center for Polymers and Advanced Composites Auburn UniversityAuburn36849ALUnited States of America Department of Chemical Engineering Auburn UniversityAuburn36849ALUnited States of America Forest Products Development Center Auburn UniversityAuburn36849ALUnited States of America Department of Biosystems Engineering Auburn UniversityAuburn36849ALUnited States of America

Bio-based phenol-formaldehyde polymer (BioNovolac) was developed by reacting molar excess of bio-oil/phenolwith formaldehyde in acidic medium. Glycidyl 3,5-diglycidoxybenzoate (GDGB), was prepared by directglycidylation of α-resorcylic acid (RA), a naturally occurring phenolic monomer. GDGB was crosslinked in thepresence of BioNovolac by anionic polymerization. Fourier transform infrared spectroscopy (FTIR) confirmedthe formation of semi-interpenetrating polymer networks. The glass transition temperature and moduli of biobasedcrosslinked systems were observed to increase with increasing GDGB content. Active chain density andmass retention measured by dynamic mechanical analysis (DMA) and Soxhlet extraction, respectively, indicated ahigh crosslink density of the cured networks. Scanning electron microscopy (SEM) images depicted thehomogeneity of the bulk phase. The preparation of bio-based epoxy-novolac thermoset network resulted inreduced consumption of petroleum-based chemicals.

关键词： Fast pyrolysis bio-oil BioNovolac semi-interpenetrating polymer networks

Generation of Eco-Friendly and Disease-Resistant Channel Catfish(Ictalurus punctatus)Harboring the Alligator Cathelicidin Gene via CRISPR/Cas9 Engineering

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Engineering 2024年第8期39卷 273-286页

作者： Jinhai Wang Baofeng Su De Xing Timothy J.Bruce Shangjia Li Logan Bern Mei Shang Andrew Johnson Rhoda Mae C.Simora Michael Coogan Darshika U.Hettiarachchi Wenwen Wang Tasnuba Hasin Jacob Al-Armanazi Cuiyu Lu Rex A.Dunham School of Fisheries Aquacultureand Aquatic SciencesAuburn UniversityAuburnAL 36849USA

As a precise and versatile tool for genome manipulation,the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)platform holds promise for modifying fish traits of *** the aim of reducing transgene introgression and controlling reproduction,upscaled disease resistance and reproductive intervention in catfish species have been studied to lower the potential environmental risks of the introgression of escapees as transgenic *** advantage of the CRISPR/Cas9-mediated system,we succeeded in integrating the cathelicidin gene(As-Cath)from an alligator(Alligator sinensis)into the target luteinizing hormone(lh)locus of channel catfish(Ictalurus punctatus)using two delivery systems assisted by double-stranded DNA(dsDNA)and single-stranded oligodeoxynucleotides(ssODNs),*** this study,high knock in(KI)efficiency(22.38%,64/286)but low ontarget events was achieved using the ssODN strategy,whereas adopting a dsDNA as the donor template led to an efficient on-target KI(10.80%,23/213).The on-target KI of As-Cath was instrumental in establishing the lh knockout(LH^(–)_As-Cath^(+))catfish line,which displayed heightened disease resistance and reduced fecundity compared with the wild-type(WT)sibling ***,administration of human chorionic gonadotropin(HCG)and luteinizing hormone-releasing hormone analogue(LHRHa)can restore the reproduction of the transgenic fish ***,we replaced the lh gene with an alligator cathelicidin transgene and then administered hormone therapy to move towards complete reproductive control of diseaseresistant transgenic catfish in an environmentally responsible *** strategy not only effectively improves consumer-valued traits but also guards against unwanted introgression,providing a breakthrough in aquaculture genetics to confine fish reproduction and prevent the establishment of transgenic or domestic genotypes in the natural environment.

关键词： Genome editing ssODN dsDNA Antimicrobial peptide Reproductive confinement Aquaculture