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Journal of World Architecture 2020年第1期4卷 1-8页

作者： Silvio Cristiano Camilla Falchetti Department of Science and Technology Universitàdegli Studi di Napoli'Parthenope’Naples80143Italy Department of Molecular Science and Nanosystems UniversitàCa’Foscari VeneziaVenice30172Italy associazione labsus–laboratorio per la sussidiarietà Turin10100Italy associazione Psicologi nel Mondo Torino Turin10100Italy

Globalized cultures,imaginaries,and economies are often matched to the standardization of building ***,solutions that are not tailored for a specific context tend to entail environmental,economic,and social *** human history,vernacular wisdom has produced interesting low-energy and climate-responsive architectures all around the globe:These can be seen as interesting examples in an era of global warming and overall uncertainty;at the same time,they represent accessible sustainable solutions in deprived areas of the world as well as in wealthier *** experience of participatory design and building process is here presented,carried on in a rural area of El Salvador,Central America.A sanitary facility for a school is designed and realized after the collectively desired recovery and improvement of one of the fruits of local vernacular wisdom in building design,i.e.,the adobe earth *** potentials and limits of such experience as well as of the used technology are reviewed in the perspective of affordable and ecologically sustainable housing and settlements in the area.

关键词： Bioclimatic buildings Vernacular architecture Urban planning Adobe earth brick Ecological sustainability

A review of the literature on the use of CRISPR/Cas9 gene therapy to treat hepatocellular carcinoma

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Oncology Research 2024年第3期32卷 439-461页

作者： ELHAM AMJAD RAFAELE PEZZANI BABAK SOKOUTI Biotechnology Research Center Tabriz University of Medical SciencesTabriz***Iran Phytotherapy lab Endocrinology UnitDipartimento di Medicina(DIMED)University of PadovaVia Ospedale 105Padova35128Italy associazione Italiana per la Ricerca Oncologica Di Base Associazione Italiana Per La Ricerca Oncologica Di BasePadova35128Italy

Noncoding RNAs instruct the Cas9 nuclease to site speifillyl cleave DNA in the CRISPR/Cas9 *** the high incidence of hepatocellular carcinoma(HCC),the patient's outcome is *** a result of the emergence of therapeutic resistance in HCC patients,dlinicians have faced difficulties in treating such *** addition,CRISPR/Cas9 screens were used to identify genes that improve the dlinical response of HCC *** is the objective of this article to summarize the current understanding of the use of the CRISPR/Cas9 system for the treatment of cancer,with a particular emphasis on HCC as part of the current state of ***,in order to locate recent developments in oncology research,we examined both the Scopus database and the PubMed *** ability to selectively interfere with gene expression in combinatorial CRISPR/Cas9 screening can lead to the discovery of new effective HCC treatment regimens by combining clinically approved *** resistance can be overcome with the help of the CRISPR/Cas9 *** signature genes and resistance to treatment have been uncovered by genome-scale CRISPR activation screening although this method is not without *** has been extensively examined whether CRISPR can be used as a tool for disease research and gene *** and its applications to tumor research,particularly in HCC,are examined in this study through a review of the literature.

关键词： CRISPR/Cas9 system Gene therapy Tumor Hepatocellular carcinoma Liver cancer Gene editing

New Primers for Denaturing Gradient Gel Electrophoresis Analysis of Nitrate-Reducing Bacterial Community in Soil

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Pedosphere 2013年第3期23卷 340-349页

作者： R.PASTORELLI R.PICCOLO S.SIMONCINI S.laNDI Consiglio per la Ricerca e la Sperimentazione in Agricoltura-Centro di Ricerca per l'Agrobiologia e la Pedologia CRA-ABP

The narG gene is frequently used as a molecular marker for bacterial nitrate-reducing community analysis. In this study, a new set of primers targeting the narG gene was designed and applied to semi-nested polymerase chain reaction-denaturing gradient gel electrophoresis （PCR-DGGE） assay. The potential of the new primers was verified on DNA directly extracted from soils from five different experimental sites distributed in Central and Southern Italy. Specificity of the primers was determined by excision, amplification, and sequencing of bands resolved by DGGE. A phylogenetic analysis showed the correlation between the sequences retrieved from the soils studied and the narG sequences from βand y-Proteobacteria. These primers expanded the existing molecular tools for ecological study on the size and diversity of nitrate-reducing bacterial community in soil.

关键词： DNA gene sequence narG phylogenetic analysis Proteobacteria

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1991-2020 climate normal in the European Alps:focus on high-elevation environments

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Journal of Mountain Science 2023年第8期20卷 2149-2163页

作者： NIGRELLI Guido CHIARLE Marta Istituto di Ricerca per la Protezione Idrogeologica Consiglio Nazionale delle RicercheTorino 10135Italy

Alps are an important geographical area of the European continent and,in this area,temperature increase is most ***,the 1991-2020 climate normal in the Alps has still not been thoroughly *** to fill this gap with a focus on high-elevation environments,minimum and maximum daily air temperature acquired by 23 automatic weather station were *** results show that the mean annual values of minimum and maximum temperature for the 1991-2020 climate normal in the Alps are-2.4℃ and 4.4℃,respectively,with a warming rate of 0.5℃/10 *** mean annual temperature comparison between 1961-1990 and 1971-2000,1961-1990 and 1981-2010,1961-1990 and 1991-2020 climate normal show an increase of 0.3℃,0.5℃ and 0.9℃,*** results also confirm that seasonal and annual temperatures are rising through the whole Alpine arc,mainly in summer and *** work highlights that annual minimum and maximum temperature do not seem to be affected by a positive elevation-dependent ***,a positive elevation-dependent warming in the maximum values of the annual minimum temperature was *** anthropogenic emissions maintain the trend of the last decades,the expected mean annual temperature of the 2001-2030 climate normal is-0.2℃,with an increase of 0.5℃ if compared to the 1991-2020 climate normal and with an increase of 1.5℃ if compared to the 1961-1990 climate *** study highlights the warming rate that is now present in the European Alps,provides indications on the warming rate that will occur in the coming years and highlights the importance of carrying out investigations that consider not only the last 30-year climate normal,but also the most recent 30-year climate normal by comparing them with each other.

关键词： Air temperature trend Climate normal Climate change High mountain climate Alps

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Chronic hepatitis C in hemodialysis patients:Prevalence and liver fibrosis impact in the National Center for Renal Health in peru

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iLIVER 2022年第3期1卷 141-144页

作者： P.Martin Padilla-Machaca Eduardo Luna-Victoria Ada Cabrera Juan-Carlos Gómez-De la Torre Rocio Galloso Pedro Montes Guillermo Almenara National Hospital EsSaludLimaPerú National University of San Marcos LimaPerú National Renal Health Center EsSaludLimaPerú laboratorio Clínico Roe LimaPerú Asociacion peruana para el Estudio del Hígado(APEH) LimaPerú

Background:Hepatitis C(HCV)remains a serious public health problem in high-risk persons such as patients on chronic *** aimed to estimate the serological prevalence of HCV,the HCV viral load and genotype,and liver fibrosis stage among patients on chronic hemodialysis at the National Renal Health Center in Lima,***:From June 2019 to March 2021,all patients who received chronic hemodialysis were invited to *** who provided written informed consent were *** with HCV-positive serology underwent determination of HCV viral *** from subjects with detectable viral load,underwent determination of HCV ***,HCV infected subjects underwent determination of liver fibrosis using transitional elastography(Fibroscan 402):Metavir score:F0-F1:2.5-7.5 kPa,F2:7.6-9.5 Kpa,F3:9.6-12 Kpa,F4(Cirrhosis):12.1-75 ***:Of the 303 subjects invited to participate,174(57.4%)subjects gave their written *** age was 52 years(range 22-91)and 116(66.6%)were *** serology was positive in 35.1%of patients(61/174);however,the prevalence of positive serology with detectable viral load was 20.11%(35/174).Genotype 1a was the most prevalent(85%).The majority(83.6%)of subjects with detectable viral load had values lower than 800.000 IU/***-nine of those 35 subjects underwent elastography evaluation,and 13(44.8%)of them were found to have stage F2-F4 ***:The prevalence of HCV at the largest reference center for hemodialysis in Lima remains high,with GT1a predominance,viral load usually below 800,000 IU/mL and significant liver fibrosis.

关键词： Hepatitis C Hemodialysis Liver fibrosis HCV genotype

Circulating and stool nucleic acid analysis for colorectal cancer diagnosis

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World Journal of Gastroenterology 2014年第4期20卷 957-967页

作者： Giulia De Maio Claudia Rengucci Wainer Zoli Daniele Calistri Biosciences laboratory Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori IRCCS

In recent years, the need to identify molecular markers characterized by high sensitivity and specificity in detecting and monitoring early and colorectal cancer lesions has increased. Up to now, none of the markers or panels of markers analyzed have met the rigorous standards required of a screening program. The important discovery of circulating nucleic acids in biological fluids has aroused intense scientific interest because of their usefulness in malignant and non malignant diseases. Over time, their yield and stability have been identified and compared with other “standard” biomarkers. The analysis of circulating DNA from blood and stool is a relatively simple and non-invasive procedure, representing a very attractive marker to detect genetic and epigenetic mutations and to monitor disease progression. A correlation between blood and stool biomarkers could also help to enhance currently available diagnostic approaches. However, various processing and analytic problems need to be resolved before such an approach can be applied in clinical practice.

关键词： Colorectal cancer Circulating DNA Circulating RNA Early diagnosis DNA integrity

Exploring the adjustment to parasite pressure hypothesis: differences in uropygial gland volume and haemosporidian infection in palearctic and neotropical birds

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Current Zoology 2021年第2期67卷 147-156页

作者： Sergio MAGALlaNES Anders Pape MøLLER Charlene LUJÁN-VEGA Esteban FONG Daniel VECCO Wendy FLORES-SAAVEDRA Luz GARCÍA-LONGORIAA Florentino DE LOPE JoséA.IANNACONE Alfonso MARZAL Department of Anatomy Cellular Biology and ZoologyUniversity of ExtremaduraAvda.de Elvas S/NBadajoz ES-06006Spain Ecologie Systématique Evolution UniversitéParis-SudOrsay 91400France Key laboratory for Biodiversity Science and Ecological Engineering of Ministry of Education College of Life SciencesBeiing Normal UniversityBeiing 100875China Pharmacology and Toxicology Graduate Group University of CaliforniaDavisDavisCA 95616USA EverGreen Institute-San Rafael Distrito de IndianaLoreto 16200Perú Centro Urku de Estudios Amazónicos Tarapoto 22202Perú Universidad Nacional Agraria la Molina Lima 15024Perú Molecular Ecology and Evolution lab Department of BiologyLund UniversityLund 22100Sweden laboratorio de Ingenieria Ambiental Universidad Cientifica del Sur-Villa el SalvadorLima 15067Perú laboratorio de Invertebrados Universidad Ricardo PalmaSantiago de Surco 15039Perú

Parasites are globally widespread pathogenic organisms,which impose im portant selective forces upon their ***,in accordance with the A djustm ent to parasite pressure hypothesis,it is expected that defenses among hosts vary relative to the selective pressure imposed by *** to the latitudinal gradient in diversity,species richness and abundance of parasites peak near the *** uropygial gland is an im portant defensive exocrine gland against pathogens in *** of the uropygial gland has been proposed to vary among species of birds because of divergent selection by pathogens on their ***,we should expect that bird species from the tropics should have relatively larger uropygial glands for their body size than species from higher ***,this hypothesis has not yet been ***,we analyze the size of the uropygial gland of 1719 individual birds belonging to 36 bird species from 3 Neotropical(peru)and 3 temperate areas(Spain).Relative uropygial gland volum e was 12.52%larger in bird species from the tropics than from temperate *** finding is consistent w ith the relative size of this defensive organ being driven by selective pressures imposed by *** also explored the potential role of this gland as a means of avoiding haemosporidian infection,showing that species with large uropygial glands for their body size tend to have lower mean prevalence of haemosporidian infection,regardless o f their geographical *** result provides additional support for the assum ption that secretions from the uropygial gland reduce the likelihood of becoming infected with haemosporidians.

关键词： adjustment to parasite pressure hypothesis malaria Neotropical region preen oil temperate region

Cavitation as a zero-waste circular economy process to convert citrus processing waste into biopolymers in high demand

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Journal of Bioresources and Bioproducts 2024年第4期9卷 486-494页

作者： Rosaria Ciriminna Giuseppe Angellotti Giovanna Li Petri Francesco Meneguzzo Cristina Riccucci Gabriella Di Carlo Mario Pagliaro Istituto per lo Studio dei Materiali Nanostrutturati CNRvia U.La Malfa 153Palermo90146Italy Istituto per la Bioeconomi1 CNRvia Madonna del Piano 10Sesto FiorentinoFI50019Italy Istituto per lo Studio dei Materiali Nanostrutturati CNRvia Salaria km 29.3MonterotondoRM00015Italy

Cavitation in water only, no matter whether hydrodynamic or acoustic, is a zero-waste circular economy process to convert industrial citrus processing waste into high-performance polysaccha- rides in high demand in a single-step at room temperature and ambient pressure using a modest amount of electricity as the only energy input. Following previous reports in which we used hydrodynamic cavitation, we now use an industrial acoustic sonicator to demonstrate the gen- eral viability of cavitation to convert biowaste residue of the industrial squeezing of pigmented sweet orange ( Citrus sinensis ) into highly bioactive “IntegroPectin ”pectin and micronized cellu- lose “CytroCell ”. From biomedicine through advanced composite membranes, said biomaterials hold great applicative potential. We conclude discussing the economic and technical feasibility of industrial implementation of the “CytroCav ”process.

关键词： Cavitation CytoCav CytroCell IntegroPectin Green extraction

PHYSICAL MODEL FOR THE ENGINEERING ANALYSISOF THE THERMOElaSTICLTY OF SOLID BODIES

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Chinese Journal of Mechanical Engineering 2000年第3期13卷 165-170,177页

作者： Lucia Umberto I.N.F.M.-Istituto Nazionale per la Fisica della Materia Nucl 意大利 Rivalta Sc I-15047

The thermal behaviour of the solids under elastic deformations is analysed in order to obtain a model which combines the molecular dynamics results with the global thermal behaviour of the solids under elastic transformations. The result obtained consists of the introduction of the thermal behaviour in the Parrinello-Rahman model, obtaining the thermal molecular dynamic model useful in the analysis of the thermoelastic behaviour of solids.

关键词： PHYSICAL MODEL FOR THE ENGINEERING ANALYSISOF THE THERMOElaSTICLTY OF SOLID BODIES

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Histone deacetylase inhibitors for treatment of hepatocellular carcinoma

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Acta Pharmacologica Sinica 2005年第9期26卷 1025-1033页

作者： Danila CORADINI Annalisa SperANZA UO Tumor Biology and Experimental Therapy Department of Experimental Oncology Istituto Nazionale per lo Studio e la Cura dei Tumori 20133 Milan Italy UO Tumor Biology and Experimental Therapy Department of Experimental Oncology Istituto Nazionale per lo Studio e la Cura dei Tumori 20133 Milan Italy

Hepatocellular carcinoma(HCC)is one of the most common cancers in the *** resection has been considered the optimal treatment approach,but onlya small proportion of patients are suitable candidates for surgery,and the relapserate is *** to prevent recurrence,including chemoemboliza-tionbefore and adjuvant therapy after surgery,have proven to have a limited benefit;liver transplantation is successful in treating limited-stage HCC because only aminority of patients qualify for ***,new therapeutic strat-egies are urgently *** in addition to the classical genetic mecha-nisms of deletion or inactivating point mutations,epigenetic alterations,such ashyperacetylation of the chromatin-associated histones(responsible for genesilencing),are believed to be involved in the development and progression ofHCC,novel compounds endowed with a histone deacetylase(HDAC)inhibitoryactivity are an attractive therapeutic *** particular,pre-clinical resultsobtained using HA-But,an HDAC inhibitor in which butyric acid residues areesterified to a hyaluronic acid backbone and characterized by a high affinity forthe membrane receptor CD44,indicated that this class of compounds may repre-sent a promising approach for hepatocellular carcinoma treatment.

关键词： hepatocellular carcinoma histone deace-tylase inhibitors cancer therapy