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Influence of Photoperiod over Morphometric and Hematological Parameters of Juvenile Piracanjubas （Brycon orbygnianus）

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Journal of Agricultural Science and Technology(B) 2016年第5期6卷 350-359页

作者： Monica Rodrigues Ferreira Machado] Eliane Aparecida de Andrade Estefania de Souza Andrade Daniella Aparecida de Jesus Paula Julia Alvarenga Oliveira Adriano carvalho Costa Ana Paula Peconick Luis David Solis Murgas department of Biological Science Federal University of Goias (UFG) Campus Cidade Universitaria CEP 75801-615 JataiGoias Brazil department of Veterinary Medicine Cdmpus Universitario Federal University of Lavras (UFLA) Lavras Minas Gerais CEP3 7200-000 Brazil department of Animal Science Federal Institute of Rio Verde (IFR V) Rio Verde Goias CEP 75901-970 Brazil

The aim of this study was to evaluate morphometric and hematological parameters of juveniles Piracanjubas treated with two distinct photoperiod. Twenty nine fish with 100 g, in a factorial design were performed in 18 h light：6 h dark （18L：6D） and 6L：lSD two different photoperiod, and morphometric and hematological parameters were evaluated at 45 d and 90 d after photoperiod treatment. Benzocaine 1% was used for euthanasia. Fish were weighed and measured, so as visceral fat, liver and gonads. Hematocrit, total count of white cells, lymphocytes and neutrophils were counted. Results showed that there was no significant relationship between bodyweight and standard length. Significant interaction （P = 0.00018） had been found between the fat index （FI） and gonado-somatic index （GSI） and the photoperiod and time. At 90 d, the fish kept in 18L：6D had a lower rate of fat than the fish kept in 6L：18D （P 〈 0.05）. The GSI was higher in the group 6L.＇18D compared with the 18L：6D （P 〈 0.005）. There was no interaction between photoperiod and period of treatment for total leukocyte count, however with 90 d, the total leukocyte count was decreased （P 〈 0.05）. Moreover, decrease of the hematocrit from 58.1% to 46.9% and an increase of lymphocytes from 31.5% to 43.5% had been found in fish kept in 18L：6D after 90 d （P 〈 0.05）. Fish kept in 6L：ISD presented an increase in segmented neutrophils and lymphocytes （P 〈 0.05）. It is concluded that photoperiod maintained at 6L： 18D for Piracanjubas permits a greater gonadal development and an amount of immune cells over the photoperiod maintained in 18L： 6D.

关键词： Circadian rhythm fish maturity cellular immune response photoperiod.

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Oral cancer:Health Promotion and Visual Screening—A Study Report

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Journal of cancer Therapy 2013年第8期4卷 1313-1320页

作者： Augusta P.Silveira Augusta Marques Miguel Pavao Eurico Monteiro Gabriela Pereira Joaquim Goncalves Luís Castro Maria Ines Guimaraes Bernardete carvalho Claudia Ribeiro Teresa Sequeira Faculty of Heath Sciences Fernando Pessoa UniversityPortoPortugal Centre of Health Studies and Research Coimbra UniversityCoimbraPortugal Non-Governmental Organization(NGO):Mundo A Sorrir PortoPortugal Portuguese institute for Oncology PortoPortugal Instituto Politecnico do Cavado e do Ave BarcelosPortugal Porto Portugal Well Medical Spa PortoPortugal Health Sciences department Portuguese Catholic UniversityViseuPortugal

Background: Oral cancer presents a heterogeneous geographic incidence. Annually, more than 275,000 new cases are diagnosed worldwide. In spite of the easy accessibility of oral cavity during physical examination, most malignancies are not diagnosed until late stages of disease. Methods: Oral health promotion was our main objective, risk factors were identified and oral cavity self-examination was promoted. The population considered (n = 1117) was divided in two main age groups—a youngest (individuals under 25 years old) mostly targeted for oral cancer awareness and oral cavity self examination promotion, and an older group having accumulated potential risk exposure. Results: The results obtained revealed smoking habits and fruits-vegetables consumption deficit as the highest risks factors found. Considering a Risk Factor Exposure Index analysis, individuals with secondary level of instruction and living in sub-urban areas assumed the highest risk exposure. Alcohol consumption had also contributed as a significant risk exposure. Conclusions: Some of these risk factors work as biological reward of quality of life deficit. A wide comprehension of the problem requires a multidisciplinary approach necessarily involving Health and Social Sciences in order to target the core of oral cancer health promotion. An effective epidemiological strategy must thus support three major aspects: population knowledge, sensitization and visual screening.

关键词： Oral cancer Visual Screening Oral Health Promotion Epidemiological Strategies

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Late Mesozoic Wangxiang Composite Granitic Pluton, South China Block: Implications to Magma Emplacement and Evolution from Geochemical Proxies

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Journal of Earth Science 2022年

作者： Quan Ou Jing-Yi Liu Feng Zi Bruna B.carvalho Xiao-Ping Long Jian-Qing Lai Kun Wang Zi-Qi Jiang Yi-Zhi Liu Zheng-Lin Li Hong-Yun Wang State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation and Key Laboratory of Deep-time Geography and Environment Reconstruction and Applications of Ministry of Natural Resources Institute of Sedimentary Geology Chengdu University of Technology State Key Laboratory of Continental Dynamics Northwest University Key Laboratory of Metallogenic Prediction of Nonferrous Metals and Geological Environment Monitoring Ministry of EducationSchool of Geosciences and Info-Physics Central South University School of Earth Sciences and Spatial Information Engineering Hunan University of Science and Technology Dipartimento Di Geoscienze Università Degli Studi Di Padova School of Resources and Geosciences China University of Mining and Technology Guangxi Key Laboratory of Hidden Metallic Ore deposits Exploration and College of Earth Sciences Guilin University of Technology

Composite granitic pluton with distinct units is a potential target for identifying its detailed magma evolution. Here, we present zircon U-Pb ages and Hf isotope, whole-rock major and trace element compositions and Nd-Pb isotopes of the Wangxiang composite pluton, South China. New ages obtained show that these rocks were generated in Late Jurassic(ca. 156–158 Ma). The rocks are divided into low silica(SiO2 71 wt.%, two-mica granites, garnet-bearing muscovite granites and muscovite granites). The high silica rocks are enriched in light rare earth elements(LREEs) relative to heavy REEs(HREEs)((La/Yb)N= 15.6–41.9, while the low silica rocks are not(0.7–76.6). All rocks show various negative Ti, Sr, Eu and strong positive Pb anomalies. The low silica rocks have less negative values of εNd(t)(-8.79 to-6.99),similar values of(206Pb/204Pb)i(18.155–18.346) and εHf(t)(-9.51 to-3.47, except one-12.84), compared to the high silica rocks(εNd(t)=-11.14 to-10.26;(206Pb/204Pb)i= 17.935–19.093; εHf(t) =-12.03 to-7.15, except one-2.41). Data suggest that the parental magma of the studied rocks(represented by enclaves) was produced by partial melting of a garnet-free crustal source. Subsequently those crustal magmas formed the more evolved units through assimilation and fractional crystallization processes, and fluid enrichment during the final magmatic activity. Combining our results with previous multidisciplinary studies, we propose that the key factor to control the evolution of Wangxiang composite pluton is discrete emplacement of crustal magmas by dyking.

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Impacts of Coffee Production in Agroforestry System for Sustainable development

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Journal of Agricultural Science and Technology(B) 2013年第8期3卷 535-544页

作者： Flavia Maria de Mello Bliska Patricia Helena Nogueira Turco Antonio Bliska Jfinior Daniel carvalho Nepomuceno Agronomic institute Coffee Centre S6o Paulo Agency of Agribusiness Technology Agriculture Secretary of Sao Paulo StateCampinas Sao Paulo 13012-970 Brazil department of decentralization of development Silo Paulo Agency of Agribusiness Technology Agriculture Secretary of SaoPaulo State Campinas Sao Paulo 13012-970 Brazil Faculty of Agricultural Engineering University of Campinas Av. Cdndido Rondon 501-Bar6o Geraldo Campinas S6o Paulo13083-875 Brazil

This study estimates the environmental and socioeconomic impacts of coffee production in the agroforestry system in the settlements of farmers located in the West of the state of Silo Paulo, Brazil, in the region known as Pontal of Paranapanema, aiming at the development of programs to support the growth of production sustainable coffee and programs to protect the coffee activity before the possible global climate change. The settlements are located in areas with sandy soils and highly degraded by human action Moreover, the region is considered marginal for the production of coffee, because it is subject to periods of high temperatures, prolonged drought and frost risk, which hinders the sustainability of farms. The environmental and socioeconomic impacts of agroforestry are analyzed through Ambitec-agro system, consisting of a set of indicators aimed at ex-post evaluation of the contribution of technological innovation to environmental, social and economic development of an agricultural activity. The system Ambitec involves：（1） general data collection about the technology; （2） application of questionnaires with adopters selected and insertion of data on impact indicators spreadsheets via platform MS-Excel and to obtain quantitative results and impacts partial indexes and aggregates the impact of technology selected; （3） analysis and interpretation of indices obtained, which vary between -15 and 15. The results indicate that agroforestry management can contribute to：（1） maintaining or increasing the sustainability of traditional coffee-growing regions in the case of sudden climate change; （2） the expansion of the coffee regions now considered unsuitable for the cultivation of coffee; （3） for sustainable regional development.

关键词： Climate changes sustainable development regional development coffee production.

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Concentrations of Silver Nitrate in the In Vitro development and Conservation of Passiflora gibertii ***

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American Journal of Plant Sciences 2017年第12期8卷 2944-2955页

作者： Gláucia Amorim Faria Lucas Menezes Felizardo Antonio Flávio Arruda Ferreira Paula Suares Rocha Aline Namie Suzuki Antonio da Silva Souza Tatiana Góes Junghans Maria Angélica Pereira de carvalho Costa Ana Patricia Bastos Peixoto Augusto Ramalho de Morais Beatriz Garcia Lopes Tiago Almeida de Oliveira departmentof Mathematics School of EngineeringSao Paulo State University(Unesp)Ilha SolteiraBrazil department of Civil Engineering School of EngineeringSao Paulo State University(Unesp)Ilha SolteiraBrazil Laboratory of Environmental Mutagenesis Institute of BiosciencesSao Paulo State University(Unesp)Rio ClaroBrazil Embrapa Cassava and Fruticulture Cruz das AlmasBrazil Center for Agrarian Biological and Environmental SciencesFederal University of the Recôncavo of Bahia(UFRB)Cruz das AlmasBrazil department of Statistics State University of Paraíba(UEPB)Campina GrandeBrazil department of Exact Sciences Federal University of Lavras(UFLA)LavrasBrazil

The aim of this study was to evaluate the effects of silver nitrate in the in vitro development and conservation of passion fruit plants. Experiments were carried out at the Laboratory of Culture and Tissues, in the National Cassava and Fruits Research Center (EMBRAPA), using microcuttings of Passiflora gibertii N. E. Br., previously cultivated in vitro. The microcuttings were placed in MS media supplemented with silver nitrate at concentrations of 0, 1, 2, 4 and 8 mg·L-1, and, during 30, 60 and 90 days, the following variables were identified: Shooting length (cm), number of roots, number and coloration of leaves. A completely randomized design with 30 replications in a split-plot scheme was employed. Polynomial regression equations were adjusted in analysis of variance to compare averages of treatments. The obtained results support the assumption of high sensitivity of passion fruit tissues to ethylene, which reflects in the lost of vigor and in the induction of culture senescence by its accumulation. Based on obtained results, and in order to mitigate this problem, it is possible to indicate an addition of 2 mg·L-1?of silver nitrate in culture media, for the micropropagation of passion fruit plant, allowing the controlling of the atmosphere inside the culture test tubes, during the establishment of explants and other steps of the process.

关键词： Passion Fruits Tropical Fruits Plant Propagation Reduced Growth Ethylene

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Relationship between clinical remission of perianal fistulas in Crohn’s disease and serum adalimumab concentrations:A multicenter cross-sectional study

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World Journal of Gastroenterology 2022年第9期28卷 961-972页

作者： Laura Sirmai Anne-Laure Pelletier Nathalie Gault Camille Zallot Guillaume Bouguen Dominique Bouchard Pascale Roland Nicaise Marine Peyneau Sandrine Sironneau Marcelo de carvalho Bittencourt Antoine Petitcollin Pedro Fernandez Xavier Roblin Laurent Siproudhis Laurent Abramowitz Division of Hepato-Gastroenterology and Digestive Oncology Bichat Claude Bernard University HospitalParis 75018France Division of Hepato-Gastroenterology Hospital Croix Saint SimonParis 75020France Division of Epidemiology Biostatistics and Clinical ResearchUniversity Hospital Center BichatParis 75018France National institute of Health and Medical Research CIC-EC1425 University Hospital Center BichatParis 75018France Division of Gastroenterology Nancy Regional and University Hospital CenterNancy 54035France Imphy CIC 1414 Group and Division of Gastroenterology and Hepatology University Hospital of RennesPontchaillouRennes 35033France Division of Proctology Hospital BagatelleTalence 33400France Division of Immunology University Hospital Center BichatParis 75018France Inflammation Microbiome and ImmunosurveillanceFaculty of PharmacyUniversitéParis-SaclayChâtenay-Malabry 92290France Division of Radiology Bagatelle Hospital CenterTalence 33400France Division of Immunology Nancy Regional and University Hospital CenterNancy 54000France University of Lorraine CNRS UMR 7365IMoPANancy 54000France Antoine Petitcol department of Clinical and Biological Pharmacology and Pharmacovigilance Pharmacoepidemiology and Drug Information CenterRennes University HospitalRennes 35700France Division of Radiology University Hospital Center BichatParis 75018France Orangerie Center Le Perreux-sur-Marne 94170France Division of Gastroenterology CHU Saint EtienneSaint-Priest-en-Jarez 42270France Division of Gastroenterology and Hepatology and Proctology University Hospital Center BichatParis 75018France Ramsay GDS Clinique Blomet Paris 75018France

BACKGROUND Crohn’s disease(CD)is complicated by perianal fistulas in approximately 20%of *** permanent fistula closure remains a challenge for *** association between serum anti-tumor necrosis factor-αconcentrations and clinical outcomes in patients with CD has been demonstrated;however,little information is available on serum adalimumab(ADA)concentrations and remission of perianal fistulas in such *** To study the relationship between serum ADA concentrations and clinical remission of CDassociated perianal *** This cross-sectional study of patients with CD-associated perianal fistulas treated with ADA was performed at four French hospitals between december 2013 and March *** the time of each serum ADA concentration measurement,we collected information about the patients and their *** primary study endpoint was clinical remission of fistulas defined as the absence of drainage(in accordance with Present’s criteria),with a PDAI≤4,absence of a seton and assessment of the overall evaluation as favorable by the proctologist at the relevant *** also assessed fistula healing[defined as being in clinical and radiological(magnetic resonance imaging,MRI)remission]and adverse *** The study cohort comprised 34 patients who underwent 56 evaluations(patients had between one and four evaluations).Fifteen patients had clinical remissions(44%),four of whom had healed fistulas on *** ADA concentrations were significantly higher at evaluations in which clinical remission was identified than at evaluations in which it was not[14(10-16)vs 10(2-15)μg/mL,P=0.01].Serum ADA concentrations were comparable at the times of evaluation of patients with and without healed fistulas[11(7-14)vs 10(4-16)μg/mL,P=0.69].The adverse event rate did not differ between different serum ADA *** We found a significant association between high serum ADA concentrations and clinical remission of CD-associated

关键词： Crohn’s disease Clinical pharmacology Peri-anal disorders Adalimumab

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Clonal replacement sustains long-lived germinal centers primed by respiratory viruses

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四川生理科学杂志 2022年第12期44卷 2169-2169页

作者： Renan V H de carvalho Laboratory of Lymphocyte Dynamics The Rockefeller University New York NY USA Weill Cornell/Rockefeller/Sloan Kettering Tri-Institutional MD-PhD Program New York NY USA Laboratory of Mucosal Immunology The Rockefeller University New York NY USA Rutgers Robert Wood Johnson Medical School and Princeton University MD/PhD Program Piscataway NJ USA Laboratory of Virology and Infectious Disease The Rockefeller University New York NY USA department of Medicine Division of Immunology and Rheumatology Stanford University School of Medicine Stanford CA USA institute for Immunity Transplantation and Infection Stanford University School of Medicine Stanford CA USA department of Epidemiology University of North Carolina at Chapel Hill Chapel Hill NC 27599 USA department of Microbiology and Immunology University of North Carolina at Chapel Hill Chapel Hill NC 27599 USA

Germinal centers(GCs)form in secondary lymphoid organs in response to infection and immunization and are the source of affinity-matured B *** duration of GC reactions spans a wide range,and long-lasting GCs(LLGCs)are ... 详细信息

关键词： organs centers respiratory

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Molecular Docking and Evaluation of Antileishmania Activity of a Ruthenium Complex with Epiisopiloturine and Nitric Oxide

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Journal of Biosciences and Medicines 2020年第5期8卷 42-53页

作者： Joabe Lima Araújo Ruan Sousa Bastos Gardênia Taveira Santos Michel Muálem de Moraes Alves Kayo Alves Figueiredo Lucas Aires de Sousa Ionara Nayana Gomes Passos Fernando Aécio de Amorim carvalho Francisco das Chagas Alves Lima Jefferson Almeida Rocha Postgraduate Program in Nanoscience and Nanobiotechnology Department of Genetics and Morphology University of Brasília Brasília DF Brazil Research Group in Natural Sciences and Biotechnology Department of Natural Sciences/Chemistry Federal University of Maranhao Grajaú MA Brazil Research Group in Medicinal Chemistry and Biotechnology QUIMEBIO Federal University of Maranhao Sao Bernardo MA Brazil Postgraduate Program in Medicinal Chemistry and Molecular Modeling Federal University of Pará Belém PA Brazil department of Nursing State University of Maranhao Colinas MA Brazil department of Veterinary Morphophysiology Federal University of Piauí Teresina PI Brazil department of Health Federal Institute of Education Science and Technology of Piauí Teresina PI Brazil Postgraduate Program in Materials Science Center for Social Sciences Health and Technology Federal University of Maranhao Imperatriz MA Brazil department of Biochemistry and Pharmacology Federal University of Piauí Teresina PI Brazil department of Chemistry Quantum Computational Chemistry Laboratory Federal University of Piauí Teresina PI Brazil

Leishmaniasis is an infectious disease that affects both animals and humans, caused by flagellated parasites belonging to the genus Leishmania. The disease is estimated to reach about 700,000 to 1 million people, causing the deaths of 20 to 30,000 individuals annually. Thus, the present study aims to perform molecular docking tests and evaluation of antileishmania activity in vitro of a ruthenium complex with epiisopiloturine and nitric oxide. AutoDockTools-1.5.6 software was used to perform molecular docking tests. Molecular targets were considered rigid, and Epiruno2 considered flexible. The genetic algorithm Lamarckian (AGL) with global search and pseudo-Solis and Wets with local search were the methods adopted in the docking. The most promising results of molecular interaction were achieved in the targets Pteridine reductase and UDP-glucose Pyrophosphorylase with rates of -10.68 Kcal·mol-1 and -10.51 Kcal·mol-1, respectively. This demonstrates that Epiruno2 has molecular affinity with the targets of L. major. In vitro assays prove the antileishmania activity of the complex in the face of promastigote forms with inhibition of growth, concluding through this study that the Epiruno2 complex has antileishmania activity.

关键词： Molecular Docking Simulation Neglected Diseases Leishmania major

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Application of Hartree-Fock Method for Modeling of Bioactive Molecules Using SAR and QSPR

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Computational Molecular Bioscience 2014年第1期4卷 1-24页

作者： Cleydson B. R. Santos Cleison C. Lobato Francinaldo S. Braga Sílvia S. S. Morais Cesar F. Santos Caio P. Fernandes Davi S. B. Brasil Lorane I. S. Hage-Melim Williams J. C. Macêdo José C. T. carvalho Laboratory of Modeling and Computational Chemistry Federal University of Amap&#225 Macap&#225 Brazil Laboratory of Modeling and Computational Chemistry Federal University of Amap&#225 Macap&#225 Brazil Laboratory of Drug Research School of Pharmaceutical Sciences Federal University of Amap&#225 Macap&#225 Brazil Laboratory of Modeling and Computational Chemistry Federal University of Amap&#225 Macap&#225 Brazil institute of Technology Federal University of Par&#225 Bel&#233m Brazil Laboratory of Modeling and Computational Chemistry Federal University of Amap&#225 Macap&#225 Brazil Postgraduate Program in Biotechnology and Biodiversity-Network BIONORTE Campus Universit&#225rio Marco Zero Macap&#225 Brazil Laboratory of Modeling and Computational Chemistry Federal University of Amap&#225 Macap&#225 Brazil Postgraduate Program in Biotechnology and Biodiversity-Network BIONORTE Campus Universit&#225rio Marco Zero Macap&#225 Brazil Laboratory of Drug Research School of Pharmaceutical Sciences Federal University of Amap&#225 Macap&#225 Brazil Laboratory of Modeling and Computational Chemistry Federal University of Amap&#225 Macap&#225 Brazil Postgraduate Program in Biotechnology and Biodiversity-Network BIONORTE Campus Universit&#225rio Marco Zero Macap&#225 Brazil Laboratory of Drug Research School of Pharmaceutical Sciences Federal University of Amap&#225 Macap&#225 Brazil Laboratory of Statistical and Computational Modeling University of the State of Amap&#225 Macap&#225 Brazil

The central importance of quantum chemistry is to obtain solutions of the Schr?dinger equation for the accurate determination of the properties of atomic and molecular systems that occurred from the calculation of wave functions accurate for many diatomic and polyatomic molecules, using Self Consistent Field method (SCF). The application of quantum chemical methods in the study and planning of bioactive compounds has become a common practice nowadays. From the point of view of planning it is important to note, when it comes to the use of molecular modeling, a collective term that refers to methods and theoretical modeling and computational techniques to mimic the behavior of molecules, not intend to reach a bioactive molecule simply through the use of computer programs. The choice of method for energy minimization depends on factors related to the size of the molecule, parameters of availability, stored data and computational resources. Molecular models generated by the computer are the result of mathematical equations that estimate the positions and properties of the electrons and nuclei, the calculations exploit experimentally, the characteristics of a structure, providing a new perspective on the molecule. In this work we show that studies of Highest Occupied Molecular Orbital Energy (HOMO), Low Unoccupied Molecular Orbital Energy (LUMO) and Map of molecular electrostatic potential (MEP) using Hatree-Fock method with different basis sets (HF/3-21G*, HF/3-21G**, HF/6-31G, HF/6-31G*, HF/6-31G** and HF/6-311G), that are of great importance in modern chemistry, biochemistry, molecular biology, and other fields of knowledge of health sciences. In order to obtain a significant correlation, it is essential that the descriptors are used appropriately. Thus, the quantum chemical calculations are an attractive source of new molecular descriptors that can, in principle, express all the geometrical and electronic properties of molecules and their interactions with biological

关键词： Molecular Modeling Meps Maps Orbital Frontier Quantum Chemical Methods

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