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Waste Tire Rubber-based Refrigerants for Solid-state Cooling devices

Chinese Journal of Polymer Science 2020年第7期38卷 769-775,I0007页

作者： Nicolau Molina Born Erik Oda Usuda Mariana da Silva Gigliotti denílson JoséMarcolino de Aguiar William Imamura Lucas Soares Paixao Alexandre Magnus Gomes carvalho Catalan institute of Nanoscience and Nanotechnology(ICN2) 08860BellaterraBarcelonaSpain The institute of Photonic Sciences(ICFO) 08193CastelldefelsBarcelonaSpain Universidade Federal de Sāo Paulo(UNIFESP) 00972-270DiademaSPBrazil Instituto de Física"Gleb Wataghin" Universidade Estadual de Campinas(UNICAMP)13083-970CampinasSPBrazil Universidade Tecnológica Federal do Paraná(UTFPR) 84017-220Ponta GrossaPRBrazil Faculdade de Engenharia Mec(a)nica Universidade Estadual de Campinas(UNICAMP)13083-860CampinasSPBrazil departamento de Engenharia Mec(a)nica Universidade Estadual de Maringá(UEM)870-900MaringáPRBrazil

Management of discarded tires is a compelling environmental issue *** there are several approaches developed to recycle waste tire rubbers,their application in solid-state cooling is still *** the high barocaloric potential verified for elastomers,the use of waste tire rubber(WTR)as a refrigerant in solid-state cooling devices is very ***,we investigated the barocaloric effects in WTR and polymer blends made of vulcanized natural rubber(VNR)and WTR,to evaluate its feasibility for solid-state cooling *** adiabatic temperature changes and the isothermal entropy changes reach giant values,as well as the performance parameters,being comparable or even better than most barocaloric materials in ***,pure WTR and WTR-based samples also present a faster thermal exchange than VNR,consisting of an additional advantage of using these discarded ***,the present findings evidence the encouraging perspectives of employing waste rubbers in solid-state cooling based on barocaloric effects,contributing to both the recycling of polymers and the sustainable energy technology field.

关键词： Waste tire rubber Solid-state cooling Barocaloric effect Tire recycling Sustainable energy

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Supergiant Barocaloric Effects in Acetoxy Silicone Rubber over a Wide Temperature Range:Great Potential for Solid-state Cooling

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Chinese Journal of Polymer Science 2020年第9期38卷 999-1005,I0008页

作者： William Imamura Erik Oda Usuda Lucas Soares Paixao Nicolau Molina Bom Angelo Marcio Gomes Alexandre Magnus Gomes carvalho Faculdade de Engenharia Mec(a)nica UNICAMPCEP 13083-860CampinasSPBrazil Universidade Federal de Sāo Paulo UNIFESPCEP 09913-030DiademaSPBrazil Instituto de Física Gleb Wataghin UNICAMPCEP 13083-859CampinasSPBrazil Catalan institute of Nanoscience and Nanotechnology ICN208860 BelaterraBarcelonaSpain The institute of Photonic Sciences ICFO08193 CastelldefelsBarcelonaSpain Instituto de Física UFRJCEP 21941-972Rio de JaneiroRJBrazil departamento de Engenharia Mec(a)nica UEMCEP 87020-900MaringáPRBrazil

Solid-state cooling based on caloric effects is considered a viable alternative to replace the conventional vapor-compression refrigeration *** barocaloric materials,recent results show that elastomers are promising candidates for cooling applications around *** the present paper,we report supergiant barocaloric effects observed in acetoxy silicone rubber—a very popular,low-cost and environmentally friendly *** values of adiabatic temperature change and reversible isothermal entropy change were obtained upon moderate applied pressures and relatively low *** huge barocaloric changes are associated both to the polymer chain rearrangements induced by confined compression and to the first-order structural *** results are comparable to the best barocaloric materials reported so far,opening encouraging prospects for the application of elastomers in near future solid-state cooling devices.

关键词： Solid-state cooling Barocaloric effect Elastomer Silicone rubber Crystalline-amorphous transition

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Bone marrow-derived monocyte infusion improves hepatic fibrosis by decreasing osteopontin,TGF-β1,IL-13 and oxidative stress

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World Journal of Gastroenterology 2017年第28期23卷 5146-5157页

作者： Veruska Cintia Alexandrino de Souza Thiago Almeida Pereira Valéria Wanderley Teixeira Helotonio carvalho Maria Carolina Accioly Brelaz de Castro Carolline Guimaraes D’assuncao Andreia Ferreira de Barros Camila Lima carvalho Virgínia Maria Barros de Lorena Vláudia Maria Assis Costa Alvaro Aguiar Coelho Teixeira Regina Celia Bressan Queiroz Figueiredo Sheilla Andrade de Oliveira Centro de Pesquisas Aggeu Magalhaes Fundacao Oswaldo Cruz Immunopathogenesis Section Laboratory of Parasitic DiseasesNational Institute of Allergy and Infectious DiseaseNational Institutes of HealthBethesdaMDUSA and Laboratório de Doencas Parasitárias e infecciosasFaculdade de MedicinaUniversidade Federal de Minas Gerais departamento de Morfologia e Fisiologia Animal Universidade Federal Rural de Pernambuco departamento de Biofísica e Radiobiologia Universidade Federal de Pernambuco Centro Acadêmico de Vitória Universidade Federal de Pernambuco departamento de Medicina Tropical Universidade Federal de Pernambuco

To evaluate the therapeutic effects of bone marrow-derived CD11b+CD14+ monocytes in a murine model of chronic liver *** liver damage was induced in C57BL/6 mice by administration of carbon tetrachloride and ethanol for 6 mo. Bone marrow-derived monocytes isolated by immunomagnetic separation were used for therapy. The cell transplantation effects were evaluated by morphometry, biochemical assessment, immunohistochemistry and enzyme-linked immunosorbent ***11b+CD14+ monocyte therapy significantly reduced liver fibrosis and increased hepatic glutathione levels. Levels of pro-inflammatory cytokines, including tumor necrosis factor-α, interleukin (IL)-6 and IL-1β, in addition to pro-fibrotic factors, such as IL-13, transforming growth factor-β1 and tissue inhibitor of metalloproteinase-1 also decreased, while IL-10 and matrix metalloproteinase-9 increased in the monocyte-treated group. CD11b+CD14+ monocyte transplantation caused significant changes in the hepatic expression of α-smooth muscle actin and *** therapy is capable of bringing about improvement of liver fibrosis by reducing oxidative stress and inflammation, as well as increasing anti-fibrogenic factors.

关键词： Monocytes Bone marrow mononuclear cells Cell therapy Macrophages Glutathione Liver fibrosis

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Inhibition of vertebrate complement system by hematophagous arthropods: inhibitory molecules, mechanisms, physiological roles, and applications

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Insect Science 2024年第5期31卷 1334-1352页

作者： Mauricio Roberto Vianna Sant'Anna Adalberto Alves Pereira-Filho Antonio Ferreira Mendes-Sousa Naylene carvalho Sales Silva Nelder Figueiredo Gontjo Marcos Horacio Pereira Leonardo Barbosa Koerich Grasielle Caldas D'Avila Pessoa John Andersen Ricardo Nascimento Araujo department of Parasitology Institute of Biological SciencesUniversidade Federal de Minas GeraisBelo HorizonteMGBrazil Instituto Nacional de Ciencia e Tecnologia em Entomologia Molecular Rio de JaneiroRBrazil Campus Senador Helvidio Nunes de Barros Universidade Federal do PiauiPicosPlBrazil Vector Molecular Biology Section Laboratory of Malaria and Vector ResearchNational Institute of Allergy and Infectious DiseasesNational Institutes of HealthRockvilleMarylandUSA

In arthropods,hematophagy has arisen several times throughout *** specialized feeding behavior offered a highly nutritious diet obtained during blood *** the other hand,blood-sucking arthropods must overcome problems brought on by blood intake and *** blood complement acts on the bite site and is still ac-tive after ingestion,so complement activation is a potential threat to the host's skin feed-ing environment and to the arthropod gut *** evolution,blood-sucking arthropods have selected,either in their saliva or gut,anticomplement molecules that inac-tivate host blood *** review presents an overview of the complement sys-tem and discusses the arthropod's salivary and gut anticomplement molecules studied to date,exploring their mechanism of action and other aspects related to the arthropod-host-pathogen *** possible therapeutic applications of arthropod's anticomplement molecules arealsodiscussed.

关键词： alternative pathway classical pathway complement inhibitors hematophagy intestine salivary gland

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Effects of climate changes on distribution of Eremanthus erythropappus and ***(Asteraceae)in Brazil

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Journal of Forestry Research 2020年第2期31卷 353-364页

作者： Lucas Fernandes Rocha Isaias Emilio Paulino do Carmo Joema Souza Rodrigues Povoa Dulcineia de carvalho departamento de Ciencias Florestais Universidade Federal de LavrasLavrasMG CEP 37200-000Brazil institute of Biosystems Engineering Poznan University of Life SciencesWojska Polskiego 5060-637 PoznanPoland

Phylogeographic patterns of endemic species are critical keys to understand its adaptation to future climate ***,based on chloroplast DNA,we analyzed the genetic diversity of two endemic and endangered tree species from the Brazilian savanna and Atlantic forest(Eremanthus erythropappus and Eremanthus incanus).We also applied the climate-based ecological niche modeling(ENM)to evaluate the impact of the Quaternary climate(last glacial maximum*21 kyr BP(thousand years before present)and Mid-Holocene*6 kyr BP)on the current haplotype ***,we modeled the potential effect of future climate change on the species distribution in 2070 for the most optimistic and pessimistic *** primer/enzyme combination(SFM/HinfI)revealed polymorphism with very low haplotype diversity,showing only three different *** haplotype 1 has very low frequency and it was classified as the oldest,diverging from six mutations from the haplotypes 2 and *** *** populations are structured and differ genetically according to the areas of *** general,the populations located in the north region are genetically different from those located in the *** genetic structuring was observed for *** ENM revealed a large distribution during the past and a severe decrease in geographic distribution of *** and *** from the LGM until present and predicts a drastic decline in suitable areas in the *** reduction may homogenize the genetic diversity and compromise a relevant role of these species on infiltration of groundwater.

关键词： Ecological niche modeling Genetic diversity Climate change Chloroplast DNA

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Selective silencing of 2Cys and type-ⅡB Peroxiredoxins discloses their roles in cell redox state and stress signaling

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Journal of Integrative Plant Biology 2015年第6期57卷 591-601页

作者： Patrícia Vidigal Ana Montserrat Martin-Hernandez Cèlia Guiu-Aragonés Sara Amncio Luísa carvalho departamento de Recursos Naturais Ambiente e Território (DRAT)/Centro de Botanica Aplicada à Agricultura (CBAA)Institute of AgronomyUniversity of Lisbon Institut de Recercai Tecnologia Agroalimentàries (IRTA) Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB) Campus Universitat Autonoma de Barcelona (UAB)Edificio Center for Research in Agricultural Genomics (CRAG)

Peroxiredoxins （Prx） catalyse the reduction of hydrogen peroxide （H2O2） and, in association with catalases and other peroxidases, may participate in signal transduction by regulating intercel ular H2O2 concentration that in turn can control gene transcription and cel signaling. Using virus-induced-gene-silencing （VIGS）, 2-Cys Peroxiredoxin （2CysPrx） family and type-II Peroxiredoxin B （PrxI B） gene were silenced in Nicotiana benthamiana, to study the impact that the loss of function of each Prx would have in the antioxidant system under control （22℃） and severe heat stress conditions （48 ℃）. The results showed that both Prxs, although in different organel es, influence the regeneration of ascorbate to a significant extent, but with different purposes. 2CysPrx affects abscisic acid （ABA） biosynthesis through ascorbate, while PrxIIB does it probably;through the xanthophyl cycle. Moreover, 2CysPrx is key in H2O2 scavenging and in consequence in the regulation of ABA signal-ing downstream of reactive oxygen species and PrxIIB provides an important assistance for H2O2 peroxisome scavenges.

关键词： 2-Cys Peroxiredoxin abscisic acid ascorbate heat stress hydrogen peroxide peroxisome type-Ⅱ Peroxiredoxin B

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Evaluating seroprevalence to circumsporozoite protein to estimate exposure to three species of Plasmodium in the Brazilian Amazon

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Infectious Diseases of Poverty 2018年第1期7卷 448-459页

作者： Virginia Araujo Pereira Juan Camilo Sanchez-Arcila Mariana Pinheiro Alves Vasconcelos Amanda Ribeiro Ferreira Lorene de Souza Videira Antonio Teva Daiana Perce-da-Silva Maria Teresa Queiroz Marques Luzia Helena de carvalho Dalma Maria Banic Luiz Cristovao Sobrino Porto Joseli Oliveira-Ferreira Laboratory of Immunoparasitology Oswaldo Cruz Institute/FiocruzAv.Brasil 4365ManguinhosRio de JaneiroBrazil institute of Infectology Emilio Ribas São PauloSao PauloBrazil Laboratory of Immunodiagnosis Departament of Biological SciencesNational School of Public Health/FiocruzRio de JaneiroBrazil Laboratory of Clinical Immunology Oswaldo Cruz Institute/FiocruzRio de JaneiroBrazil Laboratory of Histocompatibility and Cryopreservation–HLA of DHE/IBRAG/UERJ Rio de JaneiroBrazil Molecular Biology and Malaria Immunology Research Group Centro de Pesquisas Rene Rachou/FiocruzBelo HorizonteMinas GeraisBrazil

Background:Brazil has seen a great decline in malaria and the country is moving towards ***,for eventual elimination,the control program needs efficient tools in order to monitor malaria exposure and *** this study,we aimed to evaluate whether seroprevalence to the circumsporozoite protein(CSP)is a good tool for monitoring the exposure to and/or evaluating the burden and distribution of Plasmodium species in the Brazilian ***:Cross-sectional surveys were conducted in a rural area of Porto Velho,Rondônia *** infection was detected by microscopy and polymerase chain *** to the sporozoite CSP repeats of Plasmodium vivax,***,and ***(PvCS,PfCS,and PmCS)were detected using the enzyme-linked immunosorbent assay *** leukocyte antigen(HLA)-DRB1 and DQB1 genes were typed using Luminex®xMAP®***:The prevalence of immunoglobulin G against *** CSP peptide(62%)was higher than ***(49%)and ***(46%)CSP *** of the studied individuals had antibodies to at least one of the three peptides(72%),34%had antibodies to all three peptides and 28%were *** the majority of the population was not infected at the time of the survey,74.3%of parasite-negative individuals had antibodies to at least one of the ***,among individuals carrying the haplotypes DRB1*04~DQB1*03,there was a significantly higher frequency of PfCS responders,and DRB1*16~DQB1*03 haplotype for PvCS and PfCS *** contrast,HLA-DRB1*01 and HLA-DQB1*05 allelic groups were associated with a lack of antibodies to *** and *** CSP repeats,and the haplotype DRB1*01~DQB1*05 was also associated with non-responders,including non-responders to ***:Our results show that in low transmission settings,naturally acquired antibody responses against the CSP repeats of ***,***,and *** in a single cross-sectional study may n

关键词： Malaria Circumsporozoite protein Serological marker Human leucocyte antigen IgG antibody Porto Velho Rondônia Brazil

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Sex and season explain spleen weight variation in the Egyptian mongoose

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Current Zoology 2019年第1期65卷 11-20页

作者： Victor BANdeIRA Emilio Virgos Alexandre AZEVEDO Joao carvalho Monica V. CUNHA Carlos Fonseca Dartment of Biology Centro de Estudos do Ambiente e do Mar (CESAM) University of Aveiro Campus Universitario de Santiago Aveiro 3810-193 Portugal departamento de Biologia Geologia Fisica y Qufmica Inorganica Area Biodiversidad y Conservacion Escuela Superior de Ciencias Experimentales y Tecnologia (ESCET) Universidad Rey Juan Carlos c/Tulipan s/n Mostoles Madrid E-28933 Spain Leibniz institute for Zoo and Wildlife Research Alfred-Kowalke-Straβe 17 Berlin 10315 Germany Instituto de Ciencias Biomedicas Abel Salazar R. Jorge de Viterbo Ferreira 228 Porto 4050-313 Portugal Instituto Nacional de Investigaqao Agraria e Veterinaria (INIAV) IP-National Institute for Agrarian and Veterinary Research Rua dos Lagidos Lugar da Madalena Vairao 4485-655 Portugal cE3c-Centre for Ecology Evolution and Environmental Changes Faculdade de Ciencias Universidade de Lisboa Lisboa 1749-016 Portugal BiolSI-Biosystems & Integrative Sciences institute Faculdade de Ciencias Universidade de Lisboa Lisboa 1749-016 Portugal Wildlife Ecology & Health group (WE&H) and Servei d'Ecopatologia de Fauna Salvatge (SEFaS) Departament de Medicina i Cirurgia Animals Universitat Autonoma de Barcelona 08193-Bellaterra Barcelona Spain

The Egyptia n mon goose (Herpestes ichneumon Linn aeus, 1758) is a medium-sized car nivore that experienced remarkable geographic expansion over the last 3 decades in the Iberian Peninsula. In this study, we investigated the association of species-related and abiotic factors with spleen weight (as a proxy for immunocompete nee) in the species. We assessed the relationship of body con dition, sex, age, seas on, and envir onmental conditi ons with splee n weight established for 508 hunted specimens. Our results indicate that the effects of sex and season outweigh those of all other variables, including body condition. Spleen weight is higher in males than in females, and heavier spleens are more likely to be found in spring, coinciding with the highest period of investment in reproduction due to mating, gestation, birth, and lactation. Coupled with the absence of an effect of body condition, our findi ngs suggest that splee n weight variation in this species is mostly influe need by lifehistory traits linked to reproduction, rather than overall energy availability, winter immunoenhancement, or energy partitioning effects, and prompt further research focusing on this topic.

关键词： body condition carnivore Herpestes ichneumon Iberian Peninsula mongoose spleen weight

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Aberrant DNA methylation reprogramming during induced pluripotent stem cell generation is dependent on the choice of reprogramming factors

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Cell Regeneration 2014年第1期3卷 30-43页

作者： Aline C Planello Junfeng Ji Vivek Sharma Rajat Singhania Faridah Mbabaali Fabian Müller Javier A Alfaro Christoph Bock Daniel D de carvalho Nizar N Batada Campbell Family cancer Research institute Ontario Cancer InstitutePrincess Margaret Cancer CentreUniversity Health NetworkTorontoON M5G 2 M9Canada department of Morphology Piracicaba Dental SchoolUniversity of CampinasPiracicabaSPBrazil Ontario institute for cancer Research TorontoOntario M4G 0A3Canada Center of Stem Cell and developmental Biology Zhejiang UniversityHangzhouZhejiang Province 310058China National cancer institute NIHBethesdaMD 20892USA department of Medical Biophysics University of TorontoTorontoON M5G 2 M9Canada Max Planck institute for Informatics Campus E1.466123 SaarbrückenGermany CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences Lazarettgasse 141090 ViennaAustria department of Laboratory Medicine Medical University of ViennaWähringer Gürtel 18-201090 ViennaAustria

The conversion of somatic cells into pluripotent stem cells via overexpression of reprogramming factors involvesepigenetic remodeling. DNA methylation at a significant proportion of CpG sites in induced pluripotent stem cells(iPSCs) differs from that of embryonic stem cells (ESCs). Whether different sets of reprogramming factors influencethe type and extent of aberrant DNA methylation in iPSCs differently remains unknown. In order to help resolvethis critical question, we generated human iPSCs from a common fibroblast cell source using either the Yamanakafactors (OCT4, SOX2, KLF4 and cMYC) or the Thomson factors (OCT4, SOX2, NANOG and LIN28), and determinedtheir genome-wide DNA methylation profiles. In addition to shared DNA methylation aberrations present in all ouriPSCs, we identified Yamanaka-iPSC (Y-iPSC)-specific and Thomson-iPSC (T-iPSC)-specific recurrent ***, not only were the genomic locations of the aberrations different but also their types: reprogrammingwith Yamanaka factors mainly resulted in failure to demethylate CpGs, whereas reprogramming with Thomsonfactors mainly resulted in failure to methylate CpGs. Differences in the level of transcripts encoding DNMT3b andTET3 between Y-iPSCs and T-iPSCs may contribute partially to the distinct types of aberrations. Finally, de novoaberrantly methylated genes in Y-iPSCs were enriched for NANOG targets that are also aberrantly methylated insome cancers. Our study thus reveals that the choice of reprogramming factors influences the amount, location,and class of DNA methylation aberrations in iPSCs. These findings may provide clues into how to produce humaniPSCs with fewer DNA methylation abnormalities.

关键词： SOX2 programming Thomson

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Can gadoxetic acid–enhanced magnetic resonance imaging be used to avoid liver biopsy in patients with nonalcoholic fatty liver disease?

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World Journal of Hepatology 2020年第9期12卷 661-671页

作者： Viviane Brandão Amorim Daniella Braz Parente Fernando Fernandes Paiva Jaime Araújo Oliveira Neto Amanda Almeida Miranda Cláudia Cravo Moreira Flávia Ferreira Fernandes Carlos Frederico FerreiraCampos Nathalie carvalho Leite Renata de Mello Perez Rosana Souza Rodrigues Research department D’Or Institute for Research and EducationRio de Janeiro 22281Brazil Radiology department Brazilian National Cancer InstituteRio de Janeiro 20230-130Brazil Radiology department Fleury Group S.A.Rio de Janeiro 20765-000Brazil Radiology department Hospital Universitário Clementino Fraga FilhoFederal University of Rio de JaneiroRio de Janeiro 21941-590Brazil São Carlos institute of Physics University of São PauloSão Carlos 13560-970Brazil Radiology department Quinta D'Or HospitalRio de Janeiro 20941-150Brazil Radiology department Centro de Diagnóstico Médico do MaranhãoMaranhão 65074-441Brazil department of Clinical Medicine Hospital Universitário Clementino Fraga FilhoFederal University of Rio de JaneiroRio de Janeiro 21941-590Brazil Gastroenterology and Hepatology department Hospital Federal de BonsucessoRio de Janeiro 21041-030Brazil department of Pathology and Laboratories University of the State of Rio de JaneiroRio de Janeiro 20551-030Brazil Internal Medicine department Hospital Universitário Clementino Fraga FilhoFederal University of Rio de JaneiroRio de Janeiro 21941-590Brazil Gastroenterology department Hospital Universitário Pedro ErnestoUniversity of the State of Rio de JaneiroRio de Janeiro 20551-030Brazil

BACKGROUND Nonalcoholic fatty liver disease(NAFLD)is a major cause of liver disease *** diagnosis of nonalcoholic steatohepatitis(NASH),the most severe form of NAFLD,is crucial and has prognostic and therapeutic ***,currently this diagnosis is based on liver biopsy and has several *** To evaluate the performance of gadoxetic acid–enhanced magnetic resonance imaging(GA-MRI)in differentiating isolated steatosis from NASH in patients with *** In this prospective study,56 patients with NAFLD(18 with isolated steatosis and 38 with NASH)underwent *** contrast enhancement index(CEI)was calculated as the rate of increase of the liver-to-muscle signal intensity ratio from before and 20 min after intravenous GA ***-group differences in mean CEI were examined using Student's t *** area under the receiver operator characteristic curve and the diagnostic performance of gadoxetic acid–enhanced magnetic resonance imaging were *** The mean CEI for all subjects was 1.82±*** mean CEI was significantly lower in patients with NASH than in those with isolated steatosis(P=0.008).Two CEI cut-off points were used:2.00(89%sensitivity)to characterize isolated *** values between 1.66 and 2.00 indicated liver biopsy,and the procedure could be avoided in 40%of patients with *** GA-MRI is an effective noninvasive method that may be useful for the differentiation of NASH from isolated steatosis,and could help to avoid liver biopsy in patients with NAFLD.

关键词： Fatty liver Nonalcoholic fatty liver disease Nonalcoholic steatohepatitis Gadoxetic

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