检索结果-南通市图书馆

Growth,digestive and absorptive capacity and antioxidant status in intestine and hepatopancreas of sub-adult grass carp Ctenopharyngodonidella fed graded levels of dietary threonine

引用

Journal of animal Science and Biotechnology 2015年第4期6卷 424-434页

作者： Yang Hong Weidan Jiang Shengyao Kuang Kai Hu Ling Tang Yang Liu Jun Jiang Yongan Zhang Xiaoqiu Zhou Lin Feng animal nutrition Institute Sichuan Agricultural University Fish nutrition and Safety Production University Key Laboratory of Sichuan Province Sichuan Agricultural University Key Laboratory for animal Disease-Resistance nutrition of China Ministry of Education Sichuan Agricultural University Sichuan Academy of animal Science Animal Nutrition Institute Chinese Academy of Sciences Institute of Hydrobiology

Background： This study was carried out to investigate effects of threonine levels on growth, digestive and absorptive capacity and antioxidant status in intestine and hepatopancreas of sub-adult grass carp （ Ctenopharyngodonidella）. Results： Weight gain, specific growth rate, feed intake and feed efficiency were significantly improved by dietary threonine （P 〈 0.05）. Intestinal activities of trypsin, chymotrypsin, alpha-amylase, lipase, alkaline phosphatase, y-glutamyl transpeptidase and creatine kinase took the similar trends. Contents of malondialdehyde and protein carbonyl in intestine and hepatopancreas were significantly decreased by dietary optimal threonine supplementation （P 〈 0.05）. Anti-superoxide anion capacity, anti-hydroxyl radical capacity, glutathione content and activities of superoxide dismutase, catalase and glutathione-S-transferase in intestine and hepatopancreas were enhanced by dietary threonine （P 〈 0.05）. Conclusions： Dietary threonine could improve growth, enhance digestive and absorptive capacity and antioxidant status in intestine and hepatopancreas of sub-adult grass carp. The dietary threonine requirement of sub-adult grass carp （441.9-1,013.4 g） based on weight gain was 11.6 g/kg diet or 41.5 g/kg of dietary protein by quadratic regression analysis.

关键词： Antioxidant status Grass carp Intestinal enzyme activity Threonine

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of thymol and carvacrol supplementation on intestinal integrity and immune responses of broiler chickens challenged with Clostridium perfringens

引用

Journal of animal Science and Biotechnology 2016年第4期7卷 522-531页

作者： Encun Du Weiwei Wang Liping Gan Zhui Li Shuangshuang Guo Yuming Guo State Key Laboratory of animal nutrition College of Animal Science and TechnologyChina Agricultural University

Background： Necrotic enteritis caused by Clostfidium perffingens infection leads to serious economic losses in the global poultry production. In the present study, we investigated the protective effects of essential oils （EO, which contained 25 % thymol and 25 % carvacrol as active components） supplementation on growth performance, gut lesions, intestinal morphology, and immune responses of the broiler chickens infected with C. perfringens. A total of 448 1-day-old male broiler chicks were allocated into eight treatment groups following a 4 x 2 factorial arrangement with four dietary EO dosages （0, 60, 120, or 240 mg/kg） and two infection status （with or without C. perfringens challenge from d 14 to 20）. Results： The challenge did not impair the growth performance of birds, but induced gut lesions and increased crypt depth in the ileum （P ≤ 0.05）. It also down-regulated the claudin-1 and occludin mRNA expression （P ≤0.05）, up-regulated the mRNA expression of interleukin-113 （P≤ 0.05）, tended to increase the toll-like receptor （TLR） 2 mRNA expression （P 〈 0.10） in the ileum, and enhanced the mucosal secretory IgA production （P 〈 0.05）. In the challenged birds, dietary EO supplementation linearly alleviated the gut lesions and improved the ratio of villus height to crypt depth （P ≤0.05）, and the supplementation of 120 and 240 mg/kg EO increased the serum antibody titers against Newcastle disease virus （P≤ 0.05）. Regardless of challenge, the EO supplementation showed a tendency to linearly elevate the feed conversion efficiency between 14 and 28 d of age as well as the occludin mRNA expression （P〈 0.10）, and linearly inhibited the mRNA expression of TLR2 and tumor necrotic factor-o in the ileum （P≤ 0.05）. Conclusions： The dietary supplementation of EO could alleviate the intestinal injury by improving intestinal integrity and modulating immune responses in the C. perffingens-challenged broiler chickens.

关键词： Broiler chicken Clostridium peffringens Essential oils Immune response Intestinal integrity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The interaction among gut microbes,the intestinal barrier and short chain fatty acids

引用

animal nutrition 2022年第2期9卷 159-174页

作者： Jiayu Ma Xiangshu Piao Shad Mahfuz Shenfei Long Jian Wang State Key Laboratory of animal nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China Department of animal nutrition Sylhet Agricultural UniversitySylhet 3100Bangladesh

The mammalian gut is inhabited by a massive and complicated microbial community, in which the hostachieves a stable symbiotic environment through the interdependence, coordination, reciprocal constraintsand participation in an immune response. The interaction between the host gut and themicrobiota is essential for maintaining and achieving the homeostasis of the organism. Consequently, guthomeostasis is pivotal in safeguarding the growth and development and potential productive performanceof the host. As metabolites of microorganisms, short chain fatty acids are not only the preferredenergy metabolic feedstock for host intestinal epithelial cells, but also exert vital effects on antioxidantsand the regulation of intestinal community homeostasis. Herein, we summarize the effects of intestinalmicroorganisms on the host gut and the mechanisms of action of short chain fatty acids on the fourintestinal barriers of the organism, which will shed light on the manipulation of the intestinal communityto achieve precise nutrition for specific individuals and provide a novel perspective for theprevention and treatment of diseases.

关键词： Short chain fatty acids Gut microbiota Intestinal barrier Intestinal epithelium cells Interaction mechanism

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Regulatory effects of dietary L-Arg supplementation on the innate immunity and antioxidant ability in broiler chickens

引用

Journal of Integrative Agriculture 2016年第11期15卷 2578-2587页

作者： HU Ya-di TAN Jian-zhuang QI Ji ZHANG Hong-fu State Key Laboratory of animal nutrition Institute of Animal SciencesChinese Academy of Agricultural Sciences

Here, we investigated the effect of dietary arginine（Arg） supplementation on innate immunity and the antioxidant ability of broiler chickens. The experiment was designed as a single-factorial arrangement（n=8 cages/treatment, six birds/cage）, and we used four dietary Arg concentrations（10.0, 15.0, 20.0 or 25.0 g kg–1）. On day 21, the birds were killed to obtain spleen, cecal tonsil and liver samples to determine the gene expression and antioxidant characteristics. Increasing the Arg concentration linearly decreased（P0.05） the m RNA expression of splenic interleukin-18（IL-18） and tumor necrosis factor-α（TNF-α）. Dietary Arg supplementation quadratically decreased（P0.05） the expression of interleukin-1b（IL-1b） and interferon-γ（IFN-γ） m RNA in the spleen. Increasing Arg concentrations linearly and quadratically reduced the expression of IL-18 m RNA in the spleen. Meanwhile, increasing dietary Arg supplementation linearly and quadratically increased the lymphotactin m RNA（P0.05） expression, and linearly increased the macrophage inflammatory protein-1β（MIP-1β） and toll-like receptor 15（TLR15） m RNA expression in the cecal tonsils. Dietary Arg supplementation linearly（P0.05） increased the glutathione peroxidase（GSH-Px）, catalase（CAT）, and lysozyme（LZM） activities in the liver. However, the malondialdehyde（MDA） activity in the liver was not influenced by the dietary Arg concentration（P0.05）. No significant（P0.05） effect was found on the activity of superoxide dismutase（SOD） in the liver. Thus high levels of Arg supplementation（20.0 g kg^（–1）） may potentially suppress the innate immunity of broiler chickens, and dietary Arg supplementation enhances the antioxidant activity in broiler chickens.

关键词： arginine innate immunity antioxidant ability broiler

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Zinc glycine chelate ameliorates DSS‑induced intestinal barrier dysfunction via attenuating TLR4/NF‑κB pathway in meat ducks

引用

Journal of animal Science and Biotechnology 2024年第2期15卷 765-778页

作者： Yaqi Chang Ke Wang Guangmang Liu Hua Zhao Xiaoling Chen Jingyi Cai Gang Jia Institute of animal nutrition Key Laboratory for Animal Disease‑Resistance Nutrition of ChinaMinistry of EducationSichuan Agricultural UniversityChengdu 611130SichuanChina

Background Zinc glycine chelate(Zn-Gly)has anti-inflammation and growth-promoting properties;however,the mechanism of Zn-Gly contribution to gut barrier function in Cherry Valley ducks during intestinal inflammation is ***-hundred 1-day-old ducks were divided into 5 groups(6 replicates and 10 ducks per replicate)in a completely randomized design:the control and dextran sulfate sodium(DSS)groups were fed a corn-soybean meal basal diet,and experimental groups received supplements of 70,120 or 170 mg/kg Zn in form of *** DSS and treatment groups were given 2 mL of 0.45 g/mL DSS daily during d 15–21,and the control group received normal *** experiment lasted 21 *** Compared with DSS group,70,120 and 170 mg/kg Zn significantly increased body weight(BW),villus height and the ratio of villus to crypt,and significantly decreased the crypt depth of jejunum at 21 *** number of goblet cells in jejunal villi in the Zn-Gly group was significantly increased by periodic acid-Schiff *** with control,the content of intestinal permeability marker D-lactic acid(D-LA)and fluxes of fluorescein isothiocyanate(FITC-D)in plasma of DSS group significantly increased,and 170 mg/kg Zn supplementation significantly decreased the D-LA content and FITC-D *** with control,contents of plasma,jejunum endotoxin and jejunum pro-inflammatory factors IL-1β,IL-6 and TNF-αwere significantly increased in DSS group,and were significantly decreased by 170 mg/kg Zn *** Zn significantly increased the contents of anti-inflammatory factors IL-10,IL-22 and sIgA and IgG in ***-time PCR and Western blot results showed that 170 mg/kg Zn supplementation significantly increased mRNA expression levels of CLDN-1 and expression of OCLN protein in jejunum,and decreased gene and protein expression of CLDN-2 compared with DSS *** 120 mg/kg Zn significantly promoted the expressions of IL-22 and *** Zn-Gly supplementation sign

关键词： Cherry Valley ducks Growth performance Gut inflammation Intestinal barrier Zn-Gly

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

In vitro antibacterial activity of thymol and carvacrol and their effects on broiler chickens challenged with Clostridium perfringens

引用

Journal of animal Science and Biotechnology 2016年第1期7卷 95-106页

作者： Encun Du Liping Gan Zhui Li Weiwei Wang Dan Liu Yuming Guo State Key Laboratory of animal nutrition College of Animal Science and TechnologyChina Agricultural University

Background： In the post-antibiotic era, essential oils （EO） are promising alternatives to growth-promoting antibiotics. The aim of the present study was to investigate the antibacterial activities of an EO product and its components thymol and carvacrol in vitro, and the efficacy of EO to control Clostridium perffingens challenge in broiler chickens. Results： The in vitro minimum inhibitory concentration assay showed strong antibacterial activity of the EO product, thymol, and carvacrol against pathogenic Escherichia col~, C. perffingens, and Salmonella strains, and weak activity towards beneficial Lactobacillus strains. Besides, an additive effect was observed between thymol and carvacrol. The in vivo study was carried out with 448 male broiler chicks following a 4 x 2 factorial arrangement to test the effects of EO supplementation （0, 60, 120, or 240 mg/kg EO in wheat-based diet）, pathogen challenge （with or without oral gavage of C. perfr/ngens from day 14 to day 20） and their interactions. Each treatment consisted of eight replicate pens （seven birds/pen）. The challenge led to macroscopic gut lesions, and resulted in a significant increase in ileal populations of C. perffingens and Eschefichia subgroup （P ≤ 0.05） on day 21. Dietary EO supplementation did not influence C. perfringens numbers, but linearly alleviated intestinal lesions on day 21 and 28 （P= 0.010 and 0.036, respectively）, and decreased Eschefichia populations in ileum with increased EO dosages （P = 0.027 and 0.071 for day 21 and 28, respectively）. For caecum, EO quadratically influenced Lactobacillus populations on day 21 （P = 0.002）, and linearly decreased the numbers of total bacteria and Eschefichio on day 28 （P = 0.026 and 0.060, respectively）. Mean thymol and carvacrol concentrations in the small intestine were 0.21 and 0.20 μg/g in intestinal digesta （wet weight）, respectively, for birds fed 60 mg/kg EO, and 0.80 and 0.71 μg/g, respectively, for birds fed 240 mg/kg EO. Conclusions： These resu

关键词： Thymol Carvacrol Essential oils Clostfidium perfringens Eschefichia coil Broiler chickens

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Chicken gga-miR-1306-5p targets Tollip and plays an important role in host response against Salmonella enteritidis infection

引用

Journal of animal Science and Biotechnology 2019年第4期10卷 873-883页

作者： Weiwei Sun Ranran Liu Peng Li Qinghe Li Huanxian Cui Maiqing Zheng Jie Wen Guiping Zhao Institute of animal Sciences Chinese Academy of Agricultural Sciences State Key Laboratory of Animal Nutrition

Background: Increasing evidence indicates that micro RNAs(mi RNAs) are involved in inflammatory response and immune regulation following pathogen invasion. The purpose of this study was to elucidate the roles played by Gallus gallus micro RNA-1306-5 p(gga-mi R-1306-5 p) in host responses against potential invasion by Salmonella enteritidis(SE) in chickens and the underlying ***: In present study, the expression levels of gga-mi R-1306-5 p were determined in both tissues and HD11 cells. The results showed that gga-mi R-1306-5 p was significantly increased following SE infection or lipopolysaccharide(LPS) stimulation. The dual luciferase reporter assay further validated that gga-mi R-1306-5 p targeted the Toll-interacting protein(Tollip), and thereby participated in the regulation of immune response against SE or LPS stimulation through binding with the 3′-untranslated region(3’UTR) of Tollip. Additionally, the expression of Tollip was significantly blocked by over-expressed gga-mi R-1306-5 p. The underlying mechanisms by which ggami R-1306-5 p modulated the production of pro-inflammatory cytokines were also investigated. Molecular biological assays demonstrated that overexpression of gga-mi R-1306-5 p promoted the production of pro-inflammatory mediators, including NF-κB, TNF-α, IL-6, and IL-1β, which produced effects similar to those of Tollip ***: Taken together, gga-mi R-1306-5 p induced by SE or LPS, regulates the immune response by inhibiting Tollip, which activates the production of inflammatory cytokines. This study has provided the first direct evidence that gga-mi R-1306-5 p targets Tollip, and is involved in the host response against SE.

关键词： gga-miR-1306-5p LPS Pro-inflammatory cytokine Salmonella enteritidis Tollip

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of lactic acid bacteria inoculants on alfalfa(Medicago sativa L.) silage quality:assessment of degradation(in situ) and gas production(in vitro)

引用

Journal of Integrative Agriculture 2016年第12期15卷 2834-2841页

作者： LIU Ce LAI Yu-jiao LU Xiao-nan GUO Ping-ting LUO Hai-ling State Key Laboratory for animal nutrition College of Animal Science and TechnologyChina Agricultural University

Alfalfa （Medicago sativa） is difficult to ensile successfully because of the low content of moisture and water-soluble carbohydrates （WSC） in fresh alfalfa and the high buffering capacity in fresh alfalfa. Here, we conducted a study to evaluate the effects of three lactic acid bacteria （LAB） inoculants （Lactobacillus case/, lactobacillus plantarum, and Pediococcus pentosaceus） on silage quality, in sitE/ruminal degradability, and in vitro fermentation of alfalfa silage. The first cut of alfalfa was wilted, chopped, and randomly divided into four groups： the control （CON） and control mixed with three separate LAB inoculants （106 cfu g-1）. Simmental steers with a body weight of （452±18） kg and with installed rumen fistulas were prepared for in situ degradation and for in vitro gas production. LAB inoculants had a lower （P〈0.05） content of butyric acid than the CON group. Among them, the L. casei inoculated silage had a higher （P〈0.05） content of water-soluble carbohydrate （WSC） and a lower （P〈0.05） NH3-H content. The effective degradation （ED） of crude protein in LAB inoculation decreased （P〈0.05）, while the ED of acid detergent fiber increased （P〈0.05） in situ fermentation. The alfalfa silage with LAB inoculants produced more carbon dioxide （P〈0.05）. The NH3-H content of mixed incubation fluid in L. casei inoculated silage was lower （P〈0.05） compared with other groups. Therefore, this study showed that LAB inoculants could improve both ensiling quality and degradation. In particular, the L. casei inoculations exhibited better performance by limiting proteolysis during ensiling.

关键词： alfalfa silage LAB inoculants nylon-bag degradation gas production fermentation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of dietary tryptophan and stocking density on the performance,meat quality,and metabolic status of broilers

引用

Journal of animal Science and Biotechnology 2015年第1期6卷 90-96页

作者： Bo Wang Zhizhi Min Jianmin Yuan Bingkun Zhang Yuming Guo State Key Laboratory of animal nutrition College of Animal Science and TechnologyChina Agricultural University

Background： Highly automated cage-rearing systems are becoming increasingly popular in China. However, a high stocking density can cause oxidative stress and decrease broiler performance. The tryptophan （TRP） derivative 5-hydroxytryptophan （5-HT） has been shown to preserve membrane fluidity in birds suffering from oxidative stress Therefore, this experiment was conducted to determine the effects of dietan/TRP supplementation on performance, breast meat quality and oxidative stress in broilers reared in cages with a high or low stocking density. Methods： Female Arbor Acres broilers （25-d-old, n = 144） were randomly allocated to 1 of 4 treatments. The birds were fed a diet based on corn, soybean meal, cottonseed meal and corn gluten meal containing either 0.18 or 0.27% TRP and were housed with stocking densities of 11 or 15.4 birds/m2 in a 2 x 2 factorial experiment. Broiler performance was evaluated from d 25 to 42. Eight birds from each treatment were slaughtered on d 42 and plasma and breast muscle samples were collected to measure biochemical indices. Results： A higher stocking density tended to be associated with reduced weight gain （P 〈 0.10）, and significantly increased plasma glutamic-pyruvic transaminase （GPT） activity （P 〈 0.001）. Increased dietary TRP significantly reduced the activities of lactic dehydrogenase and GPT while increasing total cholesterol in the plasma （P 〈 0.01）, reducing drip loss of breast muscle （P 〈 0.10） and improving feed efficiency （P 〈 0.10）. Conclusions： An increase in dietary TRP, ].S-fold higher than the standard supplementation level, can alleviate oxidative stress as well as improve welfare and feed efficiency in broilers reared in cages with a high stocking density.

关键词： Broiler Meat quality Performance Stocking density Tryptophan

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

High amylose to amylopectin ratios in nitrogen-free diets decrease the ileal endogenous amino acid losses of broiler chickens

引用

animal nutrition 2023年第3期14卷 111-120页

作者： Huajin Zhou Tahir Mahmood Wei Wu Yanhong Chen Yao Yu Jianmin Yuan State Key Laboratory of animal nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China Adisseo animal nutrition DMCCDubai 00000United Arab Emirates

This study explored the variation of ileal endogenous amino acid(IEAA)losses and its influencing factors in chickens offered nitrogen-free diets(NFD)containing different ratios of amylose to amylopectin(AM/AP).A total of 252 broiler chickens at 28 d old were randomly allocated into 7 treatment groups for a 3-d *** dietary treatments included a basal diet(control),a NFD containing corn starch(CS),and 5NFD with AM/AP ratios of 0.20,0.40,0.60,0.80,and 1.00,*** the AM/AP ratio increased,the IEAA losses of all AAs,starch digestibility and maltase activity linearly decreased(Pnutrition and disruption of gut microbiota *** study recommends AM/AP in NFD at 0.60 to measure IEAA of broiler chickens.

关键词： Broiler chicken Endogenous amino acid losses Nitrogen-free diet Amylose Amylopectin Goblet cells

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：