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Early‑life milk replacer feeding mediates lipid metabolism disorders induced by colonic microbiota and bile acid profiles to reduce body weight in goat model

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Journal of animal Science and Biotechnology 2025年第1期16卷 300-315页

作者： Ke Zhang Ting Zhang Mengmeng Guo Awang Cuoji Yangbin Xu Yitong Zhao Yuxin Yang Daniel Brugger Xiaolong Wang Langda Suo Yujiang Wu Yulin Chen Key Laboratory of animal Genetics Breeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100China College of animal Engineering Yangling Vocational and Technical CollegeYanglingShaanxi 712100China institute of animal Sciences Tibet Academy of Agricultural and Animal Husbandry SciencesLhasa 850009China institute of animal Nutrition and Dietetics Vetsuisse‑FacultyUniversity of ZurichZurich 8057Switzerland Key Laboratory of animal Genetics and Breeding On Tibetan Plateau Ministry of Agriculture and Rural AffairsLhasa 850009China.

Background Dysregulation of lipid metabolism and its consequences on growth performance in young ruminants have attracted attention,especially in the context of alternative feeding *** study aims to elucidate the effects of milk replacer(MR)feeding on growth,lipid metabolism,colonic epithelial gene expression,colonic microbiota composition and systemic metabolism in goat kids compared to breast milk(BM)feeding,addressing a critical knowledge gap in early life *** Ten female goat kids were divided into 2 groups:those fed breast milk(BM group)and those fed a milk replacer(MR group).Over a period of 28 d,body weight was monitored and blood and tissue samples were collected for biochemical,transcriptomic and metabolomic *** of the colonial microbiota was performed using 16S rRNA gene *** microbiota transplantation(IMT)experiments in gnotobiotic mice were per-formed to validate *** MR-fed pups exhibited reduced daily body-weight gain due to impaired lipid metabolism as evidenced by lower serum and liver total cholesterol(TC)and non-esterified fatty acid(NEFA)*** analysis of the colonic epithelium revealed upregulated genes involved in negative regulation of lipid metabolism,concomitant with microbiota shifts characterized by a decrease in Firmicutes and an increase in ***,genera such as Bifidobacterium and Prevotella were enriched in the MR group,while Clostridium and Fae-calibacterium were *** analyses confirmed alterations in bile acid and fatty acid metabolic *** experiments in mice recapitulated the metabolic phenotype observed in MR-fed goats,confirming the role of the microbiota in modulating host lipid *** Milk replacer feeding in goat kids disrupts lipid metabolism and gut microbiota dynamics,result-ing in reduced growth rates and metabolic *** findings highlight the importance of e

关键词： Bile acid Colon microbiota Goat model Lipid metabolism Milk replacer

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Exposure to zearalenone exacerbates the decrease in calcium transport via selective regulation to estrogen receptor α/β in estrogenic deficient model

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Science China Life Sciences 2025年

作者： Qingxiu Liu Leli Wang Yafei Liu Shimeng Huang Lihong Zhao Yulong Yin Qiugang Ma State Key Laboratory of animal Nutrition and Feeding College of Animal Science and Technology China Agricultural University Centre of Healthy animal husbandry Institute of Subtropical Agriculture Chinese Academy of Sciences

Dear Editor,Postmenopausal estrogen deficiency in humans is thought to be the main cause of osteoporosis and diminished calcium absorption in older women. Estrogen supplementation may alleviate the problem of osteoporosis in estrogen-deficient women by promoting calcium ***, clinical estrogen supplementation increases the risk of breast cancer,and the search for other estrogen replacement is becoming of increased interest.

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Emerging patterns of antimicrobial resistance:Concurrent presence of mcr-1.1 and mcr-8.1 in a ST15 Klebsiella pneumoniae isolated from poultry in China

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Journal of Integrative Agriculture 2025年第1期24卷 403-407页

作者： Dong'an Cui Panpan Liu Ling Wang Jiongjie He Yuzhang Yan Mengke Ru Baocheng Hao Yan Sun Shengyi Wang Key Laboratory of New animal Drug Project Gansu Province/Key Laboratory of Veterinary Pharmaceutical DevelopmentMinistry of Agriculture and Rural AffairsLanzhou Institute of Husbandry and Pharmaceutical SciencesChinese Academy of Agricultural SciencesLanzhou 730050China College of animal Science and Technology Guangxi UniversityNanning 530005China China institute of veterinary Drug Control Beijing 100081China

Colistin serves as a crucial treatment for multidrugresistant(MDR)Gram-negative bacterial ***,its excessive use has contributed to an increase in colistin-resistant strains within livestock production(Binsker et al.2022).The mobile colistin resistance gene,mcr,which reduces colistin affinity by incorporating phosphoethanolamine(pEtN)into the bacterial lipopolysaccharide,significantly compromises the effectiveness of colistin treatments(Liu et al.2016).

关键词： poultry treatment compromise

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Genome-wide CRISPR screens identify CLC-2 as a drug target for anti-herpesvirus therapy:tackling herpesvirus drug resistance

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Science China(Life Sciences) 2025年第2期68卷 515-526页

作者： Fayu Yang Nan Wei Shuo Cai Jing Liu Qingping Lan Hao Zhang Lu Shang Bo Zheng Mi Wang Yingchun Liu Lifang Zhang Chenzhong Fei Wu Tong Changlong Liu Ersheng Kuang Guangzhi Tong Feng Gu Shanghai veterinary Research institute Chinese Academy of Agricultural SciencesShanghai 200241China Key Laboratory of veterinary Chemical Drugs and Pharmaceutics Ministry of Agriculture and Rural AffairsShanghai 200241China State Key Laboratory for animal Disease Control and Prevention College of Veterinary MedicineLanzhou UniversityLanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhou 730000China institute of Human Virology Zhongshan School of MedicineSun Yat-Sen UniversityGuangzhou 510080China School of Medicine Tsinghua UniversityBeijing 100084China

The emergence of drug resistance to virus(i.e.,acyclovir(ACV)to herpesviruses)has been termed one of the common clinical issues,emphasizing the discovery of new antiviral *** address it,a genome-wide clustered regularly interspaced short palindromic repeats(CRISPR)screening was performed in mouse haploid embryonic stem cells infected with pseudorabies virus(PRV),anα-herpesvirus causing human and pig *** results demonstrated that type 2 voltage-gated chloride channels(CLC-2)encoded by one of the identified genes,CLCN2,is a potential drug target for anti-herpesvirus ***-2 inhibitors,omeprazole(OME)and 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid(DIDS),can efficiently inhibit infection of multiple herpesviruses in cellulo(i.e.,PRV,HSV and EBV),and effectively treat murine herpes simplex encephalitis(HSE).Additionally,DIDS was found to inhibit HSV-1 replication by blocking the PI3K/Akt *** importantly,both DIDS and OME were able to inhibit ACV-resistant HSV-1 strain *** study's findings suggest that targeting host-cell factors such as CLC-2 may be a promising approach to tackling herpesvirus drug *** discovery of CLC-2 as a potential drug target for anti-herpesvirus therapy provides a new direction for the development of novel antiviral agents.

关键词： herpesviruses CRISPR host-directed therapy CLC-2 DIDS

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Unraveling the role of long non‑coding RNAs in chronic heat stress‑induced muscle injury in broilers

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Journal of animal Science and Biotechnology 2025年第1期16卷 206-217页

作者： Zhen Liu Yingsen Liu Tong Xing Jiaolong Li Lin Zhang Liang Zhao Yun Jiang Feng Gao College of animal Science and Technology Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu ProvinceJiangsu Collaborative Innovation Center of Meat Production and ProcessingQuality and Safety ControlNanjing Agricultural UniversityNanjing 210095People’s Republic of China Anhui Key Laboratory of Livestock and Poultry Product Safety Engineering Institute of Animal Husbandry and Veterinary MedicineAnhui Academy of Agricultural SciencesHefei 230031China institute of Agro‑Products Processing Jiangsu Academy of Agricultural SciencesNanjing 210014People’s Republic of China School of Food Science and Pharmaceutical Engineering Nanjing Normal UniversityNanjing 210023People’s Republic of China

Background Chronic heat stress(CHS)is a detrimental environmental stressor with a negative impact on the meat quality of ***,the underlying mechanisms are not fully *** study investigates the effects of CHS on long non-coding RNA(lncRNA)expression and muscle injury in broilers,with a focus on its implications for meat *** The results showed that CHS diminished breast muscle yield,elevated abdominal fat deposition,induced cellular apoptosis(P<0.05),and caused *** analysis revealed 151 differentially expressed(DE)lncRNAs when comparing the normal control(NC)and HS groups,214 DE lncRNAs when comparing the HS and PF groups,and 79 DE lncRNAs when comparing the NC and pair-fed(PF)*** eliminating the confounding effect of feed intake,68 lncRNAs were identified,primarily associated with cellular growth and death,signal transduc-tion,and metabolic ***,the apoptosis-related pathway P53,lysosomes,and the fibrosis-related gene TGF-β2 were significantly upregulated by *** These findings indicate that chronic heat stress induces cellular apoptosis and muscle injury through lncRNA,leading to connective tissue accumulation,which likely contributes to reduced breast muscle yield and meat quality in broilers.

关键词： Apoptosis Broiler Chronic heat stress LncRNA Muscle

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Seasonal influence on miRNA expression dynamics of extracellular vesicles in equine follicular fluid

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Journal of animal Science and Biotechnology 2025年第1期16卷 136-152页

作者： Jean M.Feugang Ahmed Gad Nico G.Menjivar Ghassan M.Ishak Samuel Gebremedhn Melba O.Gastal Notsile H.Dlamini Radek Prochazka Eduardo L.Gastal Dawit Tesfaye Department of animal and Dairy Sciences Mississippi State UniversityMississippi StateMS 39762USA animal Reproduction and Biotechnology Laboratory(ARBL) Department of Biomedical SciencesCollege of Veterinary Medicine and Biomedical SciencesColorado State UniversityFort CollinsCO 80523USA Department of animal Production Faculty of AgricultureCairo UniversityGiza 12613Egypt Department of Surgery and Obstetrics College of Veterinary MedicineUniversity of BaghdadBaghdad 10011Iraq animal Science School of Agricultural SciencesSouthern Illinois UniversityCarbondaleIL 62901USA J.R.Simplot Company KunaID 83634USA institute of animal Physiology and Genetics Czech Academy of SciencesLiběchov 27721Czech Republic

Background Ovarian follicular fluid(FF)is a dynamic environment that changes with the seasons,affecting follicle development,ovulation,and oocyte *** in the follicles release tiny particles called extracellular vesicles(EVs)containing vital regulatory molecules,such as microRNAs(miRNAs).These miRNAs are pivotal in facilitating commu-nication within the follicles through diverse signaling and information transfer ***-coupled miRNA signaling is implicated to be associated with ovarian function,follicle and oocyte growth and response to various environmen-tal ***,we investigated how seasonal variations directly influence the ovulatory and anovulatory states of ovarian follicles and how are they associated with follicular fluid EV-coupled miRNA dynamics in *** Ultrasonographic monitoring and follicular fluid aspiration of preovulatory follicles in horses during the ano-vulatory(spring:non-breeding)and ovulatory(spring,summer,and fall:breeding)seasons and subsequent EV isola-tion and miRNA profiling identified significant variation in EV-miRNA cargo *** identified 97 miRNAs with dif-ferential expression among the groups and specific clusters of miRNAs involved in the spring transition(miR-149,-200b,-206,-221,-328,and-615)and peak breeding period(including miR-143,-192,-451,-302b,-100,and let-7c).Bioinformatic analyses showed enrichments in various biological functions,e.g.,transcription factor activity,transcrip-tion and transcription regulation,nucleic acid binding,sequence-specific DNA binding,p53 signaling,and post-trans-lational *** analyses revealed distinct sets of significantly up-and down-regulated miRNAs associ-ated with spring anovulatory(Cluster 1)and summer ovulation–the peak breeding season(Clusters 4 and 6).Conclusions The findings from the current study shed light on the dynamics of FF-EV-coupled miRNAs in relation to equine ovulatory and anovulatory seasons,and their roles in understanding the

关键词： Extracellular vesicle Follicle growth Follicular fluid Horse Mare Ovulation Pre-ovulation Seasonal breeding

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Deletion of Salmonella pathogenicity islands SPI-1, 2 and 3 induces substantial morphological and metabolic alternation and protective immune potential

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Journal of Integrative Agriculture 2025年第1期24卷 272-289页

作者： Gaosong Liu Xuelian Lü Qiufeng Tian Wanjiang Zhang Fei Yi Yueling Zhang Shenye Yu State Key Laboratory for animal Disease Control and Prevention Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069China

The Salmonella pathogenicity islands(SPIs) play crucial roles in the progression of Salmonella infection. In this study, we constructed an improved λ Red homologous recombination system to prepare single and triple deletion mutants of 3 prominent SPIs(SPI-1, 2, and 3), aiming at the impact of deletion on morphology, carbon source metabolism, adhesion and invasion capacity, in vivo colonization, and immune efficacy in chicks. Our examination revealed that the surface of the single deletion mutants(SM6ΔSPI1, ΔSPI2, and ΔSPI3) exhibited a more rugged texture and appeared to be enveloped in a layer of transparent colloid, whereas the morphology of the triple deletion mutant(SM6ΔSPI1&2&3) remained unaltered when compared to the parent strain. The carbon metabolic spectrum of the SPI mutants underwent profound alterations, with a notable and statistically significant modification observed in 30 out of 95 carbon sources, primarily carbohydrates(17 out of 30). Furthermore, the adhesion capacity of the 4 mutants to Caco-2 cells was significantly reduced when compared to that of the parent strain. Moreover,the invasion capacity of mutants SM6ΔSPI1 and SM6ΔSPI1&2&3 exhibited a substantial decrease, while it was enhanced to varying degrees for SM6ΔSPI3 and SM6ΔSPI2. Importantly, none of the 4 mutants induced any clinical symptoms in the chicks. However, they did transiently colonize the spleen and liver. Notably, the SM6ΔSPI1&2&3mutant was rapidly cleared from both the spleen and liver within 8 days post-infection and no notable pathological changes were observed in the organs. Additionally, when challenged, the mutants immunized groups displayed a significant increase in antibody levels and alterations in the CD3+CD4+ and CD3+CD8+ subpopulations, and the levels of IL-4 and IFN-γ cytokines in the SM6ΔSPI1&2&3 immunized chicken serum surpassed those of other *** summary, the successful construction of the 4 SPI mutants lays the groundwork for further exploration into the p

关键词： Salmonella pathogenicity islands(SPls) morphology carbon source metabolism pathogenicity immunogenicity live attenuated vaccine

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Establishment of goat mammary organoid cultures modeling the mammary gland development and lactation

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Journal of animal Science and Biotechnology 2025年第1期16卷 193-205页

作者： Lei Jia Wenying Zhang Tao Luo Yongtao Li Jianhong Shu Julie Strand Yuan Yue Stig Purup Jianxin Liu Hengbo Shi Zhejiang Key Laboratory of Cow Genetic Improvement&Milk Quality Research Institute of Dairy ScienceCollege of Animal SciencesZhejiang UniversityHangzhou 310058China College of Life Sciences and Medicine Zhejiang Sci-Tech UniversityHangzhou 310018China Department of animal and veterinary Sciences Aarhus UniversityTjele DK‑8830Denmark Ministry of Education Key Laboratory of Molecular animal Nutrition Zhejiang UniversityHangzhou 310058China

Background Although several cell culture systems have been developed to investigate the function of the mam-mary gland in dairy livestock,they have potential limitations,such as the loss of alveolar structure or genetic and phe-notypic differences from their native *** these challenges is crucial for lactation *** of protocols to establish lactating organoid of livestock represents a promising goal for the *** this study,we developed a protocol to establish a culture system for mammary organoids in dairy goats to model the mammary gland development and lactation *** The organoids cultured within an extracellular matrix gel maintained a bilayer structure that closely resem-bled the native architecture of mammary *** expansion of mammary organoids was significantly promoted by growth factors containing epidermal growth factor and fibroblast growth factor 2 whereas the proliferative index of the organoids was significantly inhibited by the treatment with WNT *** stimulation with a lactogenic medium containing prolactin,the mammary organoids exhibited efficient lactation,characterized by the accumu-lation of lipid droplets in the lumen *** lactation could be sustained for more than 3 ***,the expression patterns of genes related to fatty acid synthesis and milk proteins in lactating organoids closely mirrored those observed in mammary *** observations were confirmed by data from proteomic analysis that the bulk of milk proteins was produced in the lactating *** This study is the first to establish a mammary organoid culture system modeling the mammary gland development and lactation process in *** efficient induction of lactation in ruminant mammary organoids holds promises for advancing the field of cell-based milk bio-manufacture in the food industry.

关键词： Cell-based milk production Lactation Mammary gland Organoid

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Reproductive aging in farm animals: Impacts on fertility and strategies for mitigation: A narrative review

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Asian pacific Journal of Reproduction 2025年第1期14卷 1-6页

作者： Akhter Rasool Sachin Irtiqa Manzoor Akshata Patil Division of animal Reproduction Gynecology and ObstetricsNational Dairy research InstituteKarnalIndia Division of veterinary Medicine G.B.Pant University of Agriculture and TechnologyUttarakhandIndia Department of animal Genetics and Breeding National Dairy research InstituteKarnalIndia

The livestock sector is a vital component of India's agricultural economy,providing essential support to rural communities and ensuring food *** reproductive performance is critical to sustaining livestock productivity,yet age-related declines in both male and female fertility pose significant *** current review highlights that male reproductive efficiency is closely tied to semen quality,which deteriorates with age due to oxidative stress,leading to reduced fertilizing capacity and embryonic developmental ***,female reproductive longevity is predominantly influenced by ovarian health,which declines with age due to diminished oocyte quality and *** decline is exacerbated by oxidative stress and mitochondrial dysfunction,leading to reproductive senescence and reduced offspring *** the mechanisms of reproductive aging and developing strategies to mitigate its effects are crucial for enhancing livestock productivity and sustaining the agrarian economy.

关键词： Sperm Oocyte Oxidative stress Mitochondrial dysfunction Reactive oxygen species Anti-oxidants

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Bacterial indole-3-propionic acid inhibits macrophage IL-1β production through targeting methionine metabolism

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Science China Life Sciences 2025年

作者： Ziyi Han Jian Fu Aiyan Gong Wenkai Ren State Key Laboratory of Livestock and Poultry Breeding College of Animal Science South China Agricultural University animal Disease-resistant Nutrition Key Laboratory of Sichuan Province Sichuan Agricultural University China institute of veterinary Drug Control

The gut microbiota plays key roles in host health by shaping the host immune responses through their metabolites, like indole derivatives from tryptophan. However, the direct role of these indole derivatives in macrophage fate decision and the underlying mechanism remains unknown. Here, we found that bacterial indole-3-propionic acid(IPA) downregulates interleukin-1beta(IL-1β) production in M1 macrophages through inhibition of nuclear factor-kappa B(NF-κB) signaling. Mechanistically, IPA binds specifically with methionine adenosyltransferase 2A(MAT2A) to promote S-adenosylmethionine(SAM) synthesis, which facilitates the DNA methylation of ubiquitin-specific peptidase 16(USP16, a deubiquitinase), and in turn promotes Toll-like receptor 4(TLR4) ubiquitination and NF-κB inhibition. Furthermore,IPA administration attenuates sepsis in mouse models induced by lipopolysaccharides(LPS), showcasing its potential as a microbial-derived adjunct in alleviating inflammation. Collectively, our findings reveal a newly found microbial metabolite-immune system regulatory pathway mediated by IPA.

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