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Evaluation of Beauveria bassiana （Hyphomycetes） isolates as potential agents for control of Dendroctonus valens

Insect Science 2011年第2期18卷 209-216页

作者： Long-Wa Zhang Yu-Jun Liu Jian Yao Bin Wang Bo Huang Zeng-Zhi Li Mei-Zhen Fan Jiang-Hua Sun anhui Provincial Key Laboratory of Microbial Pest Control Anhui Agricultural University Heifei anhui entry-exit inspection and quaran- tine bureau Hefei State Key Laboratory of Integrated Management Agricultural Pest & Rodents Institute of Zoology Chinese Academy of Sciences Beijing China

The red turpentine beetle （RTB）, Dendroctonus valens LeConte, as a destructive invasive pest, has become one of the most economically important forest pest in China. Effective control measures are desperately needed. Entomopathogenic fungi, such as Beauveria bassiana, have shown great potential for the management of some bark beetle species. In this study, 12 isolates of B. bassiana from bark beetle were examined for biological characteristics and virulence, to assess their potential as biocontrol agents for RTB. There were significant differences （at P = 0.05） in colony growth rate, conidial yield, conidial germination, tolerance to UV light and extracellular proteases activity among the tested B. bassiana isolates. Isolates, including Bbl801, Bb1906, Bb789 and Bb773, exhibited the best characteristics, because they have faster hyphal growth rate, higher spore production and faster spore germination, higher UV tolerance and protease （Prl） production. The results of a pathogenicity test ofB. bassiana on RTB larvae showed that most isolates of B. bassiana have demonstrated high efficacy and the highest virulent isolate was Bb 1801, which killed 100% of the treated insects and had a median lethal time （LT50） of 4.60 days at a concentration of 1 ×107 conidia/mL. Therefore, isolate Bb1801 has a great potential for sustainable control of RTB in the forest. The correlation between biological characteristics and virulence of the fungal isolates is discussed and the possibility of combination of entomopathogenic fungi with semiochemicals, as one of the promising strategy for RTB control, is considered.

关键词： Beauveria bassiana biological characteristics microbial control Dendroctonus valens virulence

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Screening of DNA Barcode of quarantine Phytophthora

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Agricultural Biotechnology 2017年第1期6卷 46-51,54页

作者： Ruifang GAO Yinghui CHENG Ying WANG Ying WANG Liyun GUO Guiming ZHANG Animal and Plant inspection and quarantine Technology Center Shenzhen Entry-Exit Inspection and Quarantine Bureau Key Laboratory of Exotic Pest Detection Technology Research of Shenzhen College of Agriculture and Biotechnology China Agricultural University

Phytophthora is genus of plant-damaging Oomycetes, whose member species are capable of causing enormous economic losses on crops worldwide. In the present study, four candidate genes ITS, CO1, EF-1α and β-tubulin were tested using 123 strains of 80 species of Phytophthora to investigate the feasibility of ser- ving as DNA barcoding markers. The results showed that among the four candidate genes, ITS and CO1 had the highest success rate of PCR amplification and se- quencing, up to 100% and 96.7%. There were obvious barcode gaps in ITS, CO1 andβ-tubulin, but their frequency distributions of intra- and interspecific genetic distances were slightly overlapped. Wilcoxon rank sum test on intraspecific genetic distances of the four genes showed ITS = CO1 = β - tubulin = EF-1α indicating they bad the same effect on intraspecific discrimination, while the test on interspecific genetic distances of the four genes showed ITS 〉 C01 〉 β- tubulin 〉 EF - 1α. In summary, ITS and COl should be used in combination as the primary barcodes, β-tubulin as the complementary barcede for the identification of 11 quaran- tine Phytophthora species.

关键词： quarantine Phytophthora DNA barcoding Genetic distance Barcoding gap

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Analysis on clinically drug-used law for lung-intestine related diseases

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Journal of Traditional Chinese Medicine 2012年第4期32卷 523-528页

作者： Luan Gao Jian Wang Fenggang Li Sihua Gao Yong Deng TCM Clinic School An-hui College of Traditional Chinese Medicine Hefei Anhui230038 China School of management Hefei University ofTechnology Hefei Anhui 230009 China

OBJECTIVE： To analyze the application laws of clini- cal drugs by descriptive statistical method with the clinical literatures guided by ＂lung being connect- ed with large intestine＂ used as the object. METHODS： Retrieve publicly published clinical litera- tures about ＂lung being connected with large intes- tine＂ in recent 30 years, establish the data base of clini- cal literature, and study on the clinically drug-used laws by descriptive statistical analytic method. RESULTS： The common high frequent drugs used for the lung-intestine related diseases were Gualou （Ructus Trichsanthis）, Dahuang （Radix et Rhizoma）, Kuxingren （Semen Armeniacae Amarum）, Gancao （Radix Glycyrrhizae）, Houpu （Cortex Magnoliae Offi- cinalis）, Zhishi （Fructus Aurantii Immaturus）. For the different clinical syndrome types, in the commonly used drugs there were different characteristics, re- flecting the principle of TCM syndrome differentia- tion ***： The common high frequent drugs used for the lung-intestine related diseases are Gua- Iou （Fructus Trichsantffis）, Dahuang （Radix et Rhi- zoma）, Kuxingren （Semen Armeniacae Amarum）, Gancao （Radix Glycyrrhizae）, Houpu （Cortex Mag- noliae Officinalis）, Zhishi （Fructus Aurantii Immatu- rus）, which are commonly-used drugs for the lung-intestine related diseases ,with differences for different syndrome types.

关键词： Lung being connected with large intes-tine Lung-intestine related diseases Drug-usedlaw

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Enhanced electrochemical property of FePO_4-coated LiNi_(0.5)Mn_(1.5)O_4 as cathode materials for Li-ion battery

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Science Bulletin 2017年第14期62卷 1004-1010页

作者： Ting-Feng Yi Yan-Mei Li Xiao-Ya Li Jing-Jing Pan Qianyu Zhang Yan-Rong Zhu School of Chemistry and Chemical Engineering Anhui University of Technology Key Laboratory of Metallurgical Emission Reduction & Resources Recycling Ministry of Education Anhui University of Technology School of Environment and Civil Engineering Dongguan University of Technology

Pristine LiNi0.5Mnl.5O4 and FePO4-coated one with Fd-3m space groups were prepared by a sol-gel method. The structure and performance were studied by X-ray diffraction （XRD） rietveld refinement, scanning electron microscopy （SEM）, high resolution transmission electron microscopy （HRTEM）, energy dispersive spectrometer （EDS） mapping, electrochemical impedance spectroscopy （EIS） and charge- discharge tests, respectively. The lattice parameters of all samples almost remain the same from the Rietveld refinement, revealing that the crystallographic structure has no obvious difference between pris- tine LiNi0.5Mn1.5O4 and FePO4-coated one. All materials show similar morphologies with uniform particle distribution with small particle size, and FePO4 coating does not affect the morphology of LiNi0.5Mnl05O 4 material. EDS mapping and HRTEM show that FePO4 may be successfully wrapped around the surfaces of ***.s04 particles, and provides an effective coating layer between the electrolyte and the surface of LiNi0.5Mn1.5O4 particles. FePO4 （1 wt%）-coated ***.504 cathode shows the highest discharge capac- ity at high rate （2 C） among all samples. After 80 cycles, the reversible discharge capacity of FePO4 （1 wt%） coated LiNi0.5Mn0.5O4 is 117 mAh g^-1, but the pristine one only has 50 mAh g^-1. FeP04 coating is an effec- tive and controllable way to stabilize the LiNi0.5Mn1.5O4/electrolyte interface, and avoids the direct con- tact between LiNi0.5Mn1.5O4 powders and electrolyte, then suppresses the side reactions and enhances the electrochemical performance of the LiNi0.5Mn1.5O4.

关键词： LiNi0.5Mn1.5O4 FePO4 coating Electrochemical property Lithium-ion batteries

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MoS2 yolk-shell microspheres with a hierarchical porous structure for efficient hydrogen evolution

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Nano Research 2016年第10期9卷 3038-3047页

作者： Qiqi Zhang Hua Bai Qing Zhang Qiang Ma Yahui Li Chongqing wan Guangcheng Xi Department of Chemistry Capital Normal University No. 105 North Road West 3th Ring Road Beijing 100048 China Nanomaterials and Nanoproducts Research Center Chinese Academy of Inspection and Quarantine No. 11 Ronghua South Road Beijing 100176 China

Yolk-shell architectures have attracted extensive attention owing to their unique structure and infusive applications. MoS2 is regarded as one of the most promising catalytic materials for hydrogen evolution by the splitting of water. In this work, a simple self-template solvothermal approach is developed for the synthesis of novel MoS2 yolk-shell microspheres with a hierarchical porous structure by reacting MoO2 microspheres with L-cysteine. A dissolution- recrystallization formation mechanism is proposed for the MoS2 yolk-shell microspheres. Owing to structural superiority, the new material architecture exhibits improved photoelectrochemical properties, including efficient hydrogen evolution reaction catalytic activities, a high photocurrent density, a small overpotential, and a low charge-transfer resistance.

关键词： yolk-shell structure hydrogen molybdenum sulfide photoelectrochemical electrochemical

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Literature-based analysis on relationship of symptoms,drugs and therapies in treatment of intestinal diseases

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Journal of Traditional Chinese Medicine 2014年第1期34卷 106-114页

作者： Luan Gao Jian Wang Yong Deng Fenggang Li Sihua Gao Traditional Chinese Medicine Clinical College of anhui Traditional Chinese Medicine University Department of Automation and Systems Technology of Aalto University Management College of Hefei Industrial University Basic Medicine College of BeijingTraditional Chinese Medicine University

OBJECTIVE:To evaluate clinical articles published in the past 30 years using the Traditional Chinese Medicine(TCM)theory of the lung being connected with large intestine to treat intestinal *** also analyzed the relationship between symptoms,drugs and therapies with data-excavating technology to aid ***:After retrieving relevant clinical articles,we set up a database,used Microsoft Structured Query Language Server 2005 Analysis Services as a data-excavating tool,and applied the association rule to study the relationship between the symptoms,drugs and therapies of intestinal ***:The key symptoms of dyschesia,constipation,abdominal fullness,fatigue and pale tongue could be treated with Kuxingren(Semen Armeniacae Amarum),Huangqi(Radix Astragali Mongolici)and Gualou(Fructus et Semen Trichosanthis)to invigorate Qi and moisten the *** these agents,Kuxingren(Semen Armeniacae Amarum)was used most *** Fu-organs was the most prevalent therapy for abdominal fullness,dyschesia,constipation and red *** Fu-organs could be achieved with Kuxingren(Semen Armeniacae Amarum)and Gualou(Fructus et Semen Trichosanthis),whereas Qi could be invigorated using Huangqi(Radix Astragali Mongolici),Gancao(Radix Glycyrrhizae),Baizhu(Rhizoma Atractylodis Macrocephalae)and Kuxingren(Semen Armeniacae Amarum).Moistening the intestine was possible with Kuxingren(SemenArmeniacae Amarum),Huomaren(Fructus Cannabis)and Jiegeng(Radix Platycodi).Also,moistening the lungs was done with Kuxingren(Semen Armeniacae Amarum),ventilating the lungs with Kuxingren(Semen Armeniacae Amarum)and Gualou(Fructus et Semen Trichosanthis),and nourishing the lungs with Huangqi(Radix Astragali Mongolici),Gancao(Radix Glycyrrhizae)and Kuxingren(Semen Armeniacae Amarum).These data demonstrated that Kuxingren(SemenArmeniacaeAmarum)was a key ***:Our analyses of the literature showed clear relationships between symptoms(constipation,dyschesia,a

关键词： Lung being connected with large intes-tine Intestinal diseases Information storage and re-trieval Data mining

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