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Synthetic and Systems Biotechnology 2024年第3期9卷 503-512页

作者： Bing Fu Meng Chen Xianfeng Bao Jiajie Lu Zhiwen Zhu Fuyao Guan Chuyang Yan Peize Wang Linglin Fu Ping Yu College of Food science and Biotechnology Zhejiang Gongshang University149 Jiaogong RoadHangzhouZhejiang Province310035People’s Republic of China College of Forestry science and Technology Lishui Vocational and Technical College357 Zhongshan Street NorthLishuiZhejiang Province323000People’s Republic of China Lishui institute for Quality Inspection and Testing 395 Zhongshan StreetLishuiZhejiang Province323000People’s Republic of China

Vitamin B_(2)is an essential water-soluble *** most prokaryotes,a bifunctional enzyme called FAD synthase catalyzes the successive conversion of riboflavin to FMN and *** this study,the plasmid pNEW-AZ containing six key genes for the riboflavin synthesis was transformed into strain R2 with the deleted FMN riboswitch,yielding strain *** R5 strain could produce 540.23±5.40 mg/L riboflavin,which was 10.61%higher than the R4 strain containing plasmids pET-AE and pAC-Z harboring six key *** further enhance the production of riboflavin,homology matching and molecular docking were performed to identify key amino acid residues of FAD *** point mutation sites were *** comparing riboflavin kinase activity,mutations of T203D and N210D,which respectively decreased by 29.90%and 89.32%compared to wild-type FAD synthase,were selected for CRISPR/Cas9 gene editing of the genome,generating engineered strains R203 and ***-AZ was transformed into R203,generating R6.R6 produced 657.38±47.48 mg/L riboflavin,a 21.69%increase compared to *** study contributes to the high production of riboflavin in recombinant *** BL21.

关键词： Vitamin B_(2) FAD synthase Escherichia coli BL21 Riboflavin kinase CRISPR/Cas9

Exploration of microscopic physical processes of Z-pinch by a modified electrostatic direct implicit particle-in-cell algorithm

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Chinese Physics B 2024年第9期33卷 433-442页

作者： Kaixuan Li Cheng Ning Ye Dong Chuang Xue institute of Applied Physics and Computational Mathematics Beijing 100088China

For investigating efficiently the stagnation kinetic-process of Z-pinch,we develop a novel modified electrostatic implicit particle-in-cell algorithm in radial one-dimension for Z-pinch simulation in which a small-angle cumulative binary collision algorithm is *** our algorithm,the electric field in z-direction is solved by a parallel electrode-plate model,the azimuthal magnetic field is obtained by Ampere’s law,and the term for charged particle gyromotion is approximated by the cross product of the averaged velocity and magnetic *** simulation results of 2 MA deuterium plasma shell Zpinch,the mass-center implosion trajectory agrees generally with that obtained by one-dimensional MHD simulation,and the plasma current also closely aligns with the external *** phase space diagrams and radial-velocity probability distributions of ions and electrons are *** main kinetic characteristic of electron motion is thermal equilibrium and oscillation,which should be oscillated around the ions,while that of ion motion is implosion *** the region of stagnation radius,the radial-velocity probability distribution of ions transits from the non-equilibrium to equilibrium state with the current increasing,while of electrons is basically the equilibrium *** the initial ion density and current peak are not high enough,the ions may not reach their thermal equilibrium state through collisions even in its stagnation phase.

关键词： Z-pinch particle-in-cell ion heating charged particle collisions

Zircon U-Pb ages and O-Hf isotopes of Quaternary trachytes from the East Sea:Implications for the genesis of low-δ^(18)O magmas

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Geoscience Frontiers 2024年第2期15卷 41-54页

作者： Hyun-Ok Choi Jihye Oh Chang Hwan Kim Soon Young Choi Won Hyuck Kim Chan Hong Park Dokdo Research Center East Sea Research InstituteKorea Institute of Ocean Science and TechnologyUljin 36315Republic of Korea Deep-Sea and Seabed Mineral Resources Research Center Korea Institute of Ocean Science and TechnologyBusan 49111Republic of Korea Division of science Education Kangwon National University1 Gangwondaehak-gilChuncheon-siGangwon-do 24341Republic of Korea

Quaternary intraplate magmatism formed several volcanic islands and seamounts,including Dokdo(DD),Ulleungdo(UD),Simheungtack(ST),Anyongbok,and Isabu in the southwest of the East Sea back-arc *** this study,we present whole-rock geochemical,zircon U–Pb age,and in situ O–Hf isotope data for the submerged volcanic rocks from DD,UD,and ST to provide new insights into the eruption timing of these volcanoes and constrain the magma evolution *** samples used in this study were trachytes and exhibited ferroan,alkalic,and metaluminous to weakly peraluminous *** showed light rare earth element(REE)-enriched patterns with(La/Yb)N ratios of 25.3–31.7 and mostly negative Eu anomalies in a chondrite-normalized REE *** addition,they were enriched in large-ion lithophile elements and high field strength elements;they exhibited positive Pb anomalies and strongly negative Ba,Sr,P,and Ti *** zircons yielded a weighted-mean 206Pb/238U age of 2.61,0.348–0.704,and 2.76–2.94 Ma for the DD,UD,and ST trachytes,*** zircons exhibited lowerδ^(18)O values than normal depleted mantle values,regardless of the crystallization age and spatial distribution of ***δ^(18)O values showed no correlation with U contents or Th/U ratios,indicating that the lowδ^(18)O signatures were of primary magmatic *** Hf isotopic compositions of the zircons were relatively heterogeneous but predominately characterized by positive eHf *** O–Hf mixing modeling revealed that low-δ^(18)O rocks with positive eHf values from the UD and ST volcanoes were derived from a hybrid source of recycled juvenile crustal materials with low-δ^(18)O and positive eHf signatures and an enriched mantle source with normalδ^(18)O and negative eHf *** juvenile oceanic crust in the source was likely metasomatized by seawater at high temperatures prior to *** contrast,the felsic magma that formed the DD volcanoes may have assimilated with regional

关键词： Quaternary intraplate volcanism East Sea Zircon O-Hf isotopes Low-δ^(18)O magma Recycled juvenile oceanic crust

Quantum discord and its dynamics for multipartite systems

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Chinese Physics B 2024年第6期33卷 168-175页

作者：罗嘉欣郭琼 College of sciences Shanghai Institute of TechnologyShanghai 201418China

Quantum discord, one of the famous quantum correlations, has been recently generalized to multipartite systems by Radhakrishnan et al. Here we give analytical solutions of the quantum discord for a family of N-qubit quantum states. For the bipartite system, we derive a zero quantum discord which will remain unchanged under the phase damping channel. For multiparitite systems, it is found that the quantum discord can be classified into three categories and the quantum discord for odd-partite systems can exhibit freezing under the phase damping channel, while the freezing does not exist in the even-partite systems.

关键词： quantum discord multipartite systems decoherence channels frozen discord

Seco-cyclic phorbol derivatives and their anti-HIV-1 activities

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Chinese Journal of Natural Medicines 2024年第4期22卷 365-374页

作者： HUANG Xiaolei HUANG Xusheng LI Qirun MA Mengdi CUI Yadong YANG Liumeng WANG Haibo LUO Ronghua CHEN Jinglei YANG Jingxuan LIN Jinrong LI Duxin ZHENG Yongtang ZHANG Jian College of Pharmaceutical science Soochow UniversitySuzhou 215123China Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of sciences/Key Laboratory of Bioactive Peptides of Yunnan Province Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China Kunming College of Life science University of Chinese Academy of SciencesKunming 650204China College of Traditional Chinese Medicine Yunnan University of Chinese MedicineKunming 650500China

Phorbol esters are recognized for their dual role as anti-HIV-1 agents and as activators of protein kinase C(PKC).The efficacy of phorbol esters in binding with PKC is attributed to the presence of oxygen groups at positions C20,C3/C4,and C9 of ***,the lipids located at positions C12/C13 are essential for both the anti-HIV-1 activity and the formation of the PKC-ligand *** influence of the cyclopropane ring at positions C13 and C14 in phorbol derivatives on their anti-HIV-1 activity requires further *** research entailed the hydrolysis of phorbol,producing seco-cyclic phorbol *** anti-HIV-1 efficacy of these derivatives was assessed,and the affinity constant(Kd)for PKC-δprotein of selected seco-cyclic phorbol derivatives was determined through isothermal titration *** findings suggest that the chemical modification of cyclopropanols could affect both the anti-HIV-1 activity and the PKC binding ***,compound S11,with an EC_(50) of 0.27μmol·L^(−1) and a CC_(50) of 153.92μmol·L^(−1),demonstrated a potent inhibitory effect on the intermediate products of HIV-1 reverse transcription(ssDNA and 2LTR),likely acting at the viral entry stage,yet showed no affinity for the PKC-δ*** results position compound S11 as a potential candidate for further preclinical investigation and for studies aimed at elucidating the pharmacological mechanism underlying its anti-HIV-1 activity.

关键词： seco-Cyclic phorbol derivatives Hydrolysis reaction Cyclopropane ring Esterification Anti-HIV-1 agent

Analysis of the discrete contact characteristics based on the Greenwood-Williamson model and the localization principle

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Friction 2024年第5期12卷 1042-1056页

作者： Anastasiya A.YAKOVENKO Irina G.GORYACHEVA Ishlinsky institute for Problems in Mechanics RAS Moscow119526Russia

The contact of a rigid body with nominally flat rough surface and an elastic half-space is *** solve the contact problem,the Greenwood-Williamson statistical model and the localization principle are *** developed contact model allows us to investigate the surface approach and the real contact area with taking into account the asperities *** is shown that the mutual influence of asperities changes not only contact characteristics at the macroscale,but also the contact pressure distribution at the *** follows from the results,the inclusion in the contact model of the effect of the mutual influence of asperities is especially significant for studying the real contact area,as well as the contact characteristics at high applied *** results calculated according to the proposed approach are in a good agreement with the experimentally observed effects,i.e.,the real contact area saturation and the additional compliance exhaustion.

关键词： surface roughness elasticity Greenwood-Williamson model localization principle asperity interaction

Modular-integrative modeling: a new framework for building brain models that blend biological realism and functional performance

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National science Review 2024年第5期11卷 13-16页

作者： Mario Senden Sacha J.van Albada Giovanni Pezzulo Egidio Falotico Ibrahim Hashim Alexander Kroner Anno C.Kurth Pablo Lanillos Vaishnavi Narayanan Cyriel Pennartz Mihai A.Petrovici Lea Steffen Tonio Weidler Rainer Goebel Department of Cognitive Neuroscience Maastricht University Maastricht Brain Imaging Centre Maastricht University institute of Neuroscience and Medicine(INM-6) and institute for Advanced Simulation(IAS-6) and JARA-Institut Brain Structure-Function Relationships(INM-10) Jülich Research Center institute of Zoology University of Cologne institute of Cognitive sciences and Technologies The Bio Robotics institute Scuola Superiore Sant'Anna RWTH Aachen University Donders institute for Brain Cognition and BehaviorRadboud University Cognitive and Systems Neuroscience Group Swammerdam Institute for Life Sciences University of Amsterdam Department of Physiology University of Bern FZI Research Center of Information Technology

To cope with the immense complexity of the brain, neuroscientific research often focuses on isolated brain structures or circumscribed perceptual, cognitive or motor functions [1]. This reductionist approach overlooks the intricate and multifaceted interplay among various structural and functional *** instance, even simple movements require the coordinated activation of numerous neuronal populations across multiple brain regions [2].

关键词： WORLD SURVEY PROJECTS

Multistage guidance on the diffusion model inspired by human artists’ creative thinking

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Frontiers of Information Technology & Electronic Engineering 2024年第1期25卷 170-178页

作者： Wang QI Huanghuang DENG Taihao LI AI Research institute Zhejiang LabHangzhou 311121China Department of Computer science and Technology Zhejiang UniversityHangzhou 310027China

Current research on text-conditional image generation shows parallel performance with ordinary painters but still has much room for improvement when compared to that of artist-ability paintings,which usually represent multilevel semantics by gathering features ofmultiple objects into one *** a preliminary experiment,we confirm this and then seek the opinions of three groups of individuals with varying levels of art appreciation ability to determine the distinctions that exist between painters and *** then use these opinions to improve an artificial intelligence(AI)painting system from painter-level image generation toward artistic-level image generation.

关键词： gathering ordinary artist

Peripheral Mechanism of Cancer-Induced Bone Pain

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Neuroscience Bulletin 2024年第6期40卷 815-830页

作者： Yachen Yang Wei Yang Ruofan Zhang Yanqing Wang Department of Integrative Medicine and Neurobiology School of Basic Medical SciencesShanghai Medical CollegeInstitute of Acupuncture ResearchInstitutes of Integrative MedicineFudan UniversityShanghai200032China Shanghai Key Laboratory of Acupuncture Mechanism and Acupoint Function Shanghai Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain ScienceInstitutes of Brain ScienceFudan UniversityShanghai200032China Zhongshan-Fudan Joint Innovation Center Zhongshan528437China

Cancer-induced bone pain(CIBP)is a type of ongoing or breakthrough pain caused by a primary bone tumor or bone *** constitutes a specific pain state with distinct characteristics;however,it shares similarities with inflammatory and neuropathic *** present,although various therapies have been developed for this condition,complete relief from CIBP in patients with cancer is yet to be ***,it is urgent to study the mechanism underlying CIBP to develop efficient analgesic ***,we focused on the peripheral mechanism associated with the initiation of CIBP,which involves tissue injury in the bone and changes in the tumor microenvironment(TME)and dorsal root *** nerve–cancer and cancer–immunocyte cross-talk in the TME creates circumstances that promote tumor growth and metastasis,ultimately leading to *** peripheral mechanism of CIBP and current treatments as well as potential therapeutic targets are discussed in this review.

关键词： Cancer-induced bone pain Peripheral mechanism Tumor microenvironment Sensory nerve Immunocytes

Integrating artificial intelligence and high-throughput phenotyping for crop improvement

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Journal of Integrative Agriculture 2024年第6期23卷 1787-1802页

作者： Mansoor Sheikh Farooq Iqra Hamadani Ambreen Kumar A Pravin Manzoor Ikra Yong Suk Chung Phenomics Laboratory Department of Plant Resources and EnvironmentJeju National UniversityJeju 63234Republic of Korea Council of Scientific&Industrial Research Indian Institute of Integrative MedicinePulwamaJ&K 192301India Animal and Dairy science University of WisconsinMadisonWI 530706United States of America Division of Fruit science Faculty of HorticultureSher-e-Kashmir University of Agricultural Sciences and Technology of KashmirSrinagar 190025India

Crop improvement is crucial for addressing the global challenges of food security and sustainable *** advancements in high-throughput phenotyping(HTP)technologies and artificial intelligence(AI)have revolutionized the field,enabling rapid and accurate assessment of crop traits on a large *** integration of AI and machine learning algorithms with HTP data has unlocked new opportunities for crop *** algorithms can analyze and interpret large datasets,and extract meaningful patterns and correlations between phenotypic traits and genetic *** technologies have the potential to revolutionize plant breeding programs by providing breeders with efficient and accurate tools for trait selection,thereby reducing the time and cost required for variety ***,further research and collaboration are needed to overcome the existing challenges and fully unlock the power of HTP and AI in crop *** leveraging AI algorithms,researchers can efficiently analyze phenotypic data,uncover complex patterns,and establish predictive models that enable precise trait selection and crop *** aim of this review is to explore the transformative potential of integrating HTP and AI in crop *** review will encompass an in-depth analysis of recent advances and applications,highlighting the numerous benefits and challenges associated with HTP and AI.

关键词： artificial intelligence crop improvement data analysis high-throughput phenotyping machine learning precision agriculture trait selection