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The Crop Journal 2024年第3期12卷 743-752页

作者： Yan Zhao Qingzhi Liu Xueran Wang Weixiao Zhang Wei Xu Yongzhong Zhang Baoshen Liu college of agronomy Shandong Agricultural UniversityTai’an 271018ShandongChina qingdao Academy of agricultural Sciences Qingdao 266100ShandongChina

The cuticular wax,acting as the ultimate defense barrier,is essential for the normal morphogenesis of plant *** this importance,the connection between wax composition and leaf development has not been thoroughly *** this study,we characterized a new maize mutant,ragged leaf4(rgd4),which exhibits crinkled and ragged leaves starting from the sixth leaf *** phenotype of rgd4 is conferred by ZmCER1,which encoding an aldehyde decarbonylase involved in wax ***1 function deficient mutant displayed reduced cuticular wax density and disordered bulliform cells(BCs),while ZmCER1 overexpressing plants exhibited the opposite effects,indicating that ZmCER1 regulates cuticular wax biosynthesis and BCs ***,as the density of cuticular wax increased,the water loss rate of detached leaf decreases,suggesting that ZmCER1 is positively correlated with plant drought tolerance.

关键词： Ragged leaf ZmCER1 Cuticular wax Bulliform cells

Candidate genes conferring ethylene-response in cultivated peanuts determined by BSA-seq and fine-mapping

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The Crop Journal 2024年第3期12卷 856-865页

作者： Yanyan Tang Zhong Huang Shaohui Xu Wenjie Zhou Jianjun Ren Fuxin Yu Jingshan Wang Wujun Ma Lixian Qiao college of agronomy Qingdao Agricultural UniversityQingdao 266109ShandongChina Murdoch university Food Futures InstitutePerthWA 6150Australia The Characteristic Laboratory of Crop Germplasm Innovation and Application Provincial Department of EducationCollege of AgronomyQingdao Agricultural UniversityQingdao 266109ShandongChina Academy of Dongying Efficient agricultural Technology and Industry on Saline and Alkaline Land in Collaboration with qingdao agricultural university Dongying 257347ShandongChina

Ethylene plays essential roles in plant growth,development and stress *** ethylene signaling pathway and molecular mechanism have been studied extensively in Arabidopsis and rice but limited in ***,we established a sand-culture method to screen pingyangmycin mutagenized peanut lines based on their specific response to ethylene(“triple response”).An ethylene-insensitive mutant,inhibition of peanut hypocotyl elongation 1(iph1),was identified that showed reduced sensitivity to ethylene in both hypocotyl elongation and root *** bulked segregant analysis sequencing,a major gene related to iph1,named AhIPH1,was preliminarily mapped at the chromosome Arahy.01,and further narrowed to a 450-kb genomic region through substitution mapping strategy.A total of 7014 genes were differentially expressed among the ACC treatment through RNA-seq analysis,of which only the Arahy.5BLU0Q gene in the candidate mapping interval was differentially expressed between WT and mutant *** sequence variations,functional annotation and transcriptome analysis revealed that a predicated gene,Arahy.5BLU0Q,encoding SNF1 protein kinase,may be the candidate gene for *** gene contained two single-nucleotide polymorphisms at promoter region and was more highly expressed in iph1 than *** findings reveal a novel ethylene-responsive gene,which provides a theoretical foundation and new genetic resources for the mechanism of ethylene signaling in peanuts.

关键词： Ethylene-insensitive Hypocotyl elongation AhIPH1 Candidate gene Genetic resources

Effect of synergistic urea by nitrification inhibitor coated with resin on wheat growth and soil nitrogen supply

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Pedosphere 2024年第5期34卷 960-970页

作者： Yaru GE Yu LI Yuanjie DONG Mingrong HE Xinglong DAI college of Resources and Environment Shandong Agricultural University agronomy college Shandong Agricultural University

Combining nitrification inhibitor and urea can improve crop yield and nitrogen (N) use efficiency (NUE). However, the inhibitor easily gets inactivated in soil, making it difficult to achieve the desired effect. To develop a synergistic urea for increasing the inhibitor action time, soil N supply, and wheat growth,dicyandiamide (DCD) was coated after granulation with epoxy resin and then mixed with urea to develop new resin-coated DCD (RCD) synergistic *** electron microscopy (SEM) and hydrostatic release tests were used to evaluate the membrane microstructure and the controlled-release performance of RCD. Five fertilization treatments were set up in the field:zero-N control (CK), urea (U), urea+common DCD particles (SUD1), urea+RCD (SUD2),and urea+both common DCD particles and RCD (3:7, weight/weight)(SUD3) to investigate the effects of the DCD synergistic urea on wheat growth, yield,and NUE and soil available N content. The SEM results showed that RCD had a complete coating, smooth surface, and small and rugged channels for DCD release in the profile. The hydrostatic release test at 25?C showed that the release period of DCD was extended to ten days due to resin coating. In the three DCD synergistic urea treatments, only SUD3 resulted in a significant increase in wheat yield (18.47%) compared with U. The NUE in SUD3 was significantly higher than those in SUD2, U, and SUD1. The treatment SUD3 had higher soil available N content than the other treatments during the key wheat growth stages, while effectively reducing the risk of soil nitrate leaching during wheat maturity. In summary, SUD3, a mixture of urea, DCD particles, and RCD, was the best treatment for significantly increasing wheat growth, yield, and NUE and soil N supply.

关键词： coated DCD controlled-release fertilizer N fertilizer N use efficiency soil available N wheat yield

Insights into the dwarfing mechanism of pear(Pyrus betulaefolia) based on anatomical and structural analysis using X-ray scanning

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Horticultural Plant Journal 2024年第2期10卷 355-366页

作者： Lili Dong Chuxuan Yang Jing Wang Jingjing Li Mei Zhao Dingli Li Zhiyun Qiu Chunhui Ma Zhenhua Cui college of Horticulture Qingdao Agricultural UniversityQingdaoShandong 266109China college of Landscape and Forestry Qingdao Agricultural UniversityQingdaoShandong 266109China

The lack of a suitable rootstock to control scion growth has limited the development of high-density plantations in pear production, which is partly attributed to poor understanding of the dwarfing mechanism. In the present study, the rootstock of the dwarf-type pear (Pyrus betulaefolia)PY-9’ was identified and used as the material for anatomical ***-9’ grew to half the tree height of the normal cultivar Zhengdu’, along with fewer internodes and shorter length. Significant differences in growth rate betweenPY-9’ andZhengdu’ were detected at approximately 30 days after full bloom, which corresponded with the time of the greatest difference in water potential between the dwarf and normal ***-9’ showed a higher photosynthetic rate thanZhengdu’. Anatomical analysis showed thatPY-9’ had higher area ratios of both phloem and xylem and more developed vascular tissues thanZhengdu’. The three-dimensional reconstructed skeleton of the xylem from X-ray computed tomography scanning revealed greater intervessel connectivity inZhengdu’ than inPY-9’, which could contribute to the more vigorous growth ofZhengdu’. This study thus provides the first comparison of the microstructural properties of xylem elements between a dwarfing-type and vigorous-type pear rootstock, providing new insights into the dwarfing mechanism in pear and facilitating breeding of dwarf pear rootstocks to increase crop productivity.

关键词： Pyrus betulaefolia Anatomical analysis Dwarfing mechanism Intervessel connectivity Rootstock

Exogenous calcium enhances the physiological status and photosynthetic capacity of rose under drought stress

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Horticultural Plant Journal 2024年第3期10卷 853-865页

作者： Xiaojuan Zhao Shang Lin Shuang Yu Yichang Zhang Lin Su Lifang Geng Chenxia Cheng Xinqiang Jiang college of Landscape Architecture and Forestry Qingdao Agricultural UniversityQingdaoShandong 266000China college of Horticultural Qingdao Agricultural UniversityQingdaoShandong 266000China

Drought(water shortage)can substantially limit the yield and economic value of rose plants(Rosa spp.).Here,we characterized the effect of exogenous calcium(Ca^(2+))on the antioxidant system and photosynthesis-related properties of rose under polyethylene glycol 6000(PEG6000)-induced drought *** levels,as well as leaf and root biomass,were significantly reduced by drought;drought also had a major effect on the enzymatic antioxidant system and increased concentrations of reactive oxygen *** of exogenous Ca^(2+)increased the net photosynthetic rate and stomatal conductance of leaves,enhanced water-use efficiency,and increased the length and width of stomata following exposure to ***-specific physiological responses were observed under different concentrations of Ca^(2+).Application of 5 mmol·L^(-1)Ca^(2+)promoted photosynthesis and antioxidant activity in the leaves,and application of 10 mmol·L^(-1)Ca^(2+)promoted antioxidant activity in the *** of exogenous Ca^(2+)greatly enhanced the phenotype and photosynthetic capacity of potted rose plants following exposure to drought ***,our findings indicate that the application of exogenous Ca^(2+)enhances the drought resistance of roses by promoting physiological adaptation and that it could be used to aid the cultivation of rose plants.

关键词： Rosa hybrida L. Exogenous calcium Drought stress Physiological index Photosynthetic capacity

Fe3O4/Fe/FeS Tri-Heterojunction Node Spawning N-Carbon Nanotube Scaffold Structure for High-Performance Sodium-Ion Battery

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Energy & Environmental Materials 2024年第4期7卷 143-152页

作者： Yuan Liu Qing Lin Xiaocui Chen Xufeng Meng Baoxiu Hou Haiyan Liu Shuaihua Zhang Ningzhao Shang Zheng Wang Chaoyue Zhang Jianjun Song Xiaoxian Zhao Department of Chemistry College of Science Hebei Agricultural University college of Chemistry and Chemical Engineering Qingdao University college of Physics Qingdao University

The Fe-based anode of sodium-ion batteries attracts much attention due to the abundant source,low-cost,and high specific ***,the low electron and ion transfer rate,poor structural stability,and shuttle effect of NaS2intermediate restrain its further ***,the Fe3O4/Fe/FeS tri-heterojunction node spawned N-carbon nanotube scaffold structure（FHNCS） was designed using the modified MIL-88B（Fe） as a template followed by catalytic growth and sulfidation process.

关键词： anode core-shell heterojunction hollow structure sodium ion batteries

Examining the evolution of tidal creeks in the Huanghe River delta using multi-temporal Landsat images

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Journal of Oceanology and Limnology 2024年第4期42卷 1061-1073页

作者： Zhen HAN Kai JIN Quanli ZONG Peng QIN Chunxia LIU Xiuzhi ZHU Xinyue LI college of Resources and Environment Qingdao Agricultural UniversityQingdao 266109China

Coastal tidal creeks are important channels for exchanges of material and energy between sea and land,and play an important role in the ecological protection of tidal *** tidal creeks have evolved differently in various regions,the evolutionary process of tidal creeks in the Huanghe(Yellow)River delta of China,one of the most active deltas worldwide,is not entirely ***,the evolution of tidal creeks in the delta from 1981 to 2021 was investigated by quantitatively analysing the tidal creeks and developing a standard for dividing their evolution *** interpretation and supervised classification methods were applied to the Landsat images to extract the tidal creek network,and 17 groups of tidal creek systems were *** indicate that Creek S 1 was the most developed creek for having 113 tidal creeks totaling 65.8 km in length,while Creek E 3 had the fastest growth rate for having average annual increase of 1.9 ***,the level of tidal creeks increased,the average and median lengths of tidal creeks increased,and the number of tidal creeks decreased since *** evolution of the tidal creek system could be divided into four stages,namely,rising,developing,stabilizing,and *** of a representative tidal creek show that there was no degenerated tidal creek during the rising period,with an increase in the number of 50 and a length increase of 57.9 km between 1981 and *** proportion of new tidal creeks in the developing period was more than 50%and the new tidal creeks in the stabilizing period were equal to the degraded tidal *** tidal creeks were greater than 50%during the degrading *** was no fixed order of tidal creek evolution in each period,and there may be a skip in *** findings provided a reference for studying the evolution of tidal creeks.

关键词： tidal creek evolution pattern remote sensing Huanghe(Yellow)River delta

The role of the auxin-response genes MdGH3.1 and MdSAUR36 in bitter pit formation in apple

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Horticultural Plant Journal 2024年第5期10卷 1085-1098页

作者： Daqing Huang Wen Peng Na Gong Lina Qiu Yongzhang Wang Haiyong Qu college of Horticulture Qingdao Agricultural UniversityQingdaoShandong 266109China

Apples often exhibit bitter pits in response to metabolic disorders during ripening and storage;however, the mechanisms underlying the bitter pit(BP) development remain unclear. Here, metabolome and transcriptome analyses were performed to investigate BP pulp of 'Fuji'. Two auxin-response genes, MdGH3.1 and MdSAUR36, were screened. Their expression as well as the auxin content in BP pulp were found to be higher than those in healthy pulp(P < 0.01). In the field, excess CO(NH2)2increased the incidence of BP. Moreover, the auxin content and MdGH3.1 expression increased in apples after nitrogen fertilization. On Day 30 before harvest, the two genes were transiently transferred to the fruit, and 20.69% and 23.21% of BP fruits were harvested. After 10 μmol·L-1auxin was infiltrated at low pressure into postharvest fruit, the increase in MdGH3.1 expression occurred earlier than that in MdSAUR36. MdGH3.1 increased the expression of MdSAUR36, but MdSAUR36 did not increase expression of MdGH3.1. Therefore, we suggest that MdGH3.1 acts upstream of MdSAUR36 during BP formation and that these genes induce BP formation by regulating auxin and phenylpropanoid biosynthesis.

关键词： Apple Malusdomestica Auxin Bitter pit Flavonoids Nitrogen fertilizer Widely targeted metabolomics

Changes in milk fat globule membrane proteins along lactation stage of Laoshan dairy goat

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Journal of Integrative Agriculture 2024年第5期23卷 1737-1748页

作者： Chuozi Liang Zhongna Yu Guangming Zhu Yixuan Li Xueheng Sun Hongning Jiang Qijing Du Rongbo Fan Jun Wang Yongxin Yang Rongwei Han college of Food Science and Engineering Qingdao Agricultural UniversityQingdao 266109China Bathurst Future Agri-Tech Institute Qingdao Agricultural UniversityQingdao 266109China Haidu Cllege Qingdao Agricultural UniversityQingdao 266109China

The milk fat globule membrane(MFGM)is a complex structure with numerous functions,and its composition is affected by many *** have been few systematic investigations on goat MFGM proteome profiling during *** milk samples from 15 healthy dairy goats were obtained at six lactation time points for investigation of the MFGM proteome using both data-independent acquisition(DIA)and data-dependent acquisition(DDA)proteomics techniques combined with multivariate statistical *** the DIA method,890 variably abundant MFGM proteins were discovered throughout the lactation *** 1 to 240 d,butyrophilin subfamily 1 member A1,lipoprotein lipase,perilipin-2,and adipose triglyceride lipase were upregulated,while APOE,complement C3,clusterin,and IgG were ***,from 1 to 90 d,annexin A1,annexin A2,and antithrombin-ll were downregulated,then upregulated by d *** had a high degree of connectedness,indicating that it was a key protein,according to protein-protein interaction ***,our findings gave new insights into the biological features of MFGM protein in goat milk throughout lactation,which may aid in the creation of specialized MFGM products and infant formula.

关键词： goat milk fat globule membrane protein data-independent acquisition(DIA) data-dependent acquisition(DDA) lactation proteomics

Molecular mechanisms underlying the negative effects of transient heatwaves on crop fertility

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Plant Communications 2024年第9期5卷 101-115页

作者： Qian Yao Ping Li Xin Wang Shuhua Liao Pu Wang Shoubing Huang college of agronomy and Biotechnology China Agricultural UniversityBeijing 100193China

Transient heatwaves occurring more frequently as the climate warms,yet their impacts on crop yield are severely underestimated and even *** lasting only a few days or even hours during sensitive stages,such as microgametogenesis and flowering,can significantly reduce crop yield by disrupting plant *** advances in multi-omics and GWAS analysis have shed light on the specific organs(e.g.,pollen,lodicule,style),key metabolic pathways(sugar and reactive oxygen species metabolism,Ca2+homeostasis),and essential genes that are involved in crop responses to transient heatwaves during sensitive *** review therefore places particular emphasis on heat-sensitive stages,with pollen development,floret opening,pollination,and fertilization as the central narrative *** multifaceted effects of transient heatwaves and their molecular basis are systematically reviewed,with a focus on key structures such as the lodicule and tapetum.A number of heat-tolerance genes associated with these processes have been identified in major crops like maize and *** mechanisms and key heat-tolerance genes shared among different stages may facilitate the more precise improvement of heat-tolerant crops.

关键词： heat stress floret fertility pollen pollen tube growth seed set molecular mechanism