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Expression Variability of Carbohydrates at the Cell Wall Surface of Aspergillus Species and in Vitro Susceptibility to Amphotericin B, Voriconazole and Itraconazole

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Journal of Life Sciences 2014年第4期8卷 358-363页

作者： André Ferraz Goiana Leal Eduardo Isidoro Cameiro Beltrao Almir Goncalves Wanderley Rejane pereira Neves Department of Mycology Federal University of Pernambuco Recife PE Brazil Department of Biochemistry Federal University of Pernambuco Recife PE Brazil Keizo Asami Immunopathology Laboratory (LIKA) Federal University of Pernambuco Recife PE Brazil Department of Physiology and Pharmacology Federal University of Pernambuco Recife PE Brazil

This study evaluated the expression of N-acetyl-D-glucosamine, L-fucose, D-galactose and glucose/mannose on the cell wall surface of Aspergillus species using lectins and the in vitro antifungal activity of AMB （amphotericin B）, VOR （voriconazole） and ITC （itraconazole）. Con A （Concanavalin A）, WGA （wheat germ agglutinin）, UEA-I （Ulex europeus agglutinin I） and PNA （peanut agglutinin）, all conjugated with horseradish peroxidase, were used. For the susceptibility tests, sterilized fiat-bottomed 96-well microtitre plates were used and the procedures were followed of the Clinical and Laboratory Standard institute. The inoculum was added to the wells with the tested drugs and the plates were incubated at 25 ℃ for 48 h before reading the results to determine the MIC （minimal inhibitory concentration） of AMB, VOR and ITC. All Aspergillus strains showed low MIC for AMB （0.031-1 μg/mL） and ITC （0.031-0.25μg/mL）. However, the isolates were less susceptible to VOR, for which the MIC ranged from 4 to 16 μg/mL. The results of this study indicate that the expression of N-acetyl-D-glucosamine and glucose/mannose on the cell wall surface of the Aspergillus species evaluated showed greater stability of expression in the fungal growth both in vitro and in parasitism.

关键词： Cell wall carbohydrates lectins Aspergillus antifungal activity.

R eview of Soil and Water Assessment Tool(SWAT)applications in Brazil:C hallenges and prospects

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International Journal of Agricultural and Biological Engineering 2015年第3期8卷 9-35,F0002,I0001页

作者： Danielle de Almeida Bressiani Philip W.Gassman Josimar Gurgel Fernandes Luis Hamilton Pospissil Garbossa Raghavan Srinivasan Nadia Bernard i Bonumá Eduardo Mario Mendiondo University of São Paulo EESCSHS-São Carlos/SP 13566-590Brazil Department of Ecosystem Science and Management SSLTexas A&M UniversityCollege StationTX 77843USA Center for Agricultural and Rural Development Iowa State University-AmesIA 50011-1070USA agronomic institute of pernambuco-ipa-recife/pe 50761-000Brazil Federal University of pernambuco-UFpe-recife/pe 50670-901Brazil Santa Catarina Agricultural Research and Extension Corporation-Epagri/Ciram-Florianópolis/SC 88034-901Brazil Federal University of Santa Catarina-UFSC-Florianópolis/SC 88040-900Brazil Brazilian Center of Monitoring and Early Wa rning of Natural Disasters CEMADEN/MCTISão Josédos Campos/SP12247-016 Brazil

The geographical extent of Brazil exceeds 8.5 million km2 and encompasses a complex mix of biomes and other environmental *** decision support tools are needed to help support management of these diverse Brazilian natural resources including ecohydrological *** use of the Soil and Water Assessment Tool(SWAT)ecohydrological watershed-scale model in Brazil has increased greatly during the past *** over 100 SWAT studies were identified in this review which have been published during 1999 to 2015 in Brazilian and international journals,conference proceedings,and as theses or dissertations,many of which are written in *** majority of these studies(102 total)are reviewed here as part of an extensive survey covering the 1999 to 2013 time *** and spatial distributions,a summary of hydrologic calibration and validation results and a synopsis of the types of applications that were performed are reported for the surveyed studies.A smaller subset of recent Brazilian studies published in English between 2012 and 2015 in scientific journals are also reviewed,with emphasis on hydrologic and sediment transport testing results as well as scenario applications that were *** majority of the surveyed SWAT studies was performed for watersheds located in the South and Southeast regions of Brazil(67%)and was conducted in the context of academic *** 50%of the surveyed studies reported only hydrologic *** trends were found for the subset of more recent English *** studies have been reported that describe applications of SWAT in Brazil by private firms or government agencies;this review indicates that the potential exists for increased numbers of such studies in the ***,there is evidence that a lack of accessibility to adequate quality input data is a possible hindrance to the more general use of SWAT for watershed applications in Brazil.

关键词： SWAT models mapping applications review Brazil

Ammonium Improves Corn Phosphorus Acquisition Through Changes in the Rhizosphere Processes and Root Morphology

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pedosphere 2019年第4期29卷 534-539页

作者： Elialdo Alves DE SOUZA Natália Rodrigues FERREIRA-ELOY Camila da Silva GRASSMANN Ciro Antonio ROSOLEM Philip J.WHITE School of agronomic Sciences Sao Paulo State University The James Hutton institute

Dear Editor, Phosphorus (P) deficiency is the most limiting factor in tropical agriculture since most soils in this region are predominantly acids with high-energy P-binding compounds. Therefore, up to 90% of the P applied becomes fixed in soils, which raises environmental concerns and production costs (Syers et al., 2008). Thus, developing strategies to imptove P use efficiency (PUE) is essential to mitigate environmental problems and improve profits. These strategies should consider the soil-root-rhizosphere system because it is the most biochemical active site where nutrient uptake occurs (Hinsinger, 2011).

关键词： Root Morphology Rhizosphere Processes Dear Editor

Neglected tropical diseases in Brazilian children and adolescents:data analysis from 2009 to 2013

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Infectious Diseases of Poverty 2017年第1期6卷 1369-1378页

作者： Eduardo Brandao Sebastian Romero Maria Almerice Lopes da Silva Fred Luciano Neves Santos National Reference Service for Filariasis Aggeu Magalhães Institute(Fiocruz-PE)RecifePernambucoBrazil Faculty of Medical Sciences National University of RosarioRosarioSanta FeArgentina Laboratory of Communicable Diseases Parasitology DepartmentAggeu Magalhaes Institute(Fiocruz-PE)RecifePernambucoBrazil Laboratory of Pathology and Bio-Intervention Goncalo Moniz Institute(Fiocruz-BA)SalvadorBahiaBrazil

Background:Neglected tropical diseases(NTDs)prevail in conditions of poverty and contribute to the maintenance of social *** of the NTDs prioritized by the Brazilian Ministry of Health,four parasitic infections require mandatory notification:acute Chagas disease,leishmaniasis,malaria,and *** on the behaviour of these NTDs in the young population are currently *** study seeks to analyse the epidemiological aspects of these parasitic infections in children and adolescents in ***:A retrospective exploratory ecological study was conducted.A spatial analysis of the cases reported between 2009 and 2013 in individuals aged between 0 and 19 years that were notified through the Health Notification Aggravation Information System(SINAN)was ***:In total,64,567 cases of cutaneous and visceral leishmaniasis,malaria,schistosomiasis,and acute Chagas disease were recorded in the SINAN database,representing a rate of 20.15 cases per 100,000 *** average age of the cases was 12.2 years and 62.32%were *** hundred and three deaths related to these obligatorily reported parasites were recorded,indicating a case fatality rate of 0.62%.Visceral leishmaniasis and acute Chagas disease had the highest rates of lethality.A heterogeneous spatial distribution of the studied parasites was ***:The number of cases and the lethality rate described in this study show that these diseases still represent a serious problem for public health in *** points to the need to encourage new research and the reformulation of social,economic,and public health policies aimed at ensuring better health and living conditions for all individuals,especially those among the populations considered vulnerable,as is the case of the young.

关键词： Parasites Visceral leishmaniasis Malaria Schistosomiasis Chagas disease Disease notification Residence characteristics Brazil

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In situ-based assessment of soil liquefaction potential-Case study of an earth dam in Tunisia

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Frontiers of Structural and Civil Engineering 2014年第4期8卷 456-461页

作者： Ikram GUETTAYA Mohamed Ridha EL OUNI Department of Rural Engineering National Agronomic Institute of Tunis Tunis 1082 Tunisia

The present paper examines the evaluation of liquefaction potential of an earth dam foundation in Tunisia. The assessment of soil liquefaction was made using deterministic and probabilistic simplified procedures developed from several case histories. The data collected from the field investigation performed before and after the vibrocompaction are analyzed and the results are reported. The obtained results show that after vibrocompaction, a significant improvement of the soil resistance reduces the liquefaction potential of the sandy foundation. Indeed, in the untreated layers, the factor of safety FS drops below 1 which means that the soil is susceptible for liquefaction. However, in the compacted horizons, the values of FS exceed the unit which justifies the absence of liquefaction hazard of the foundation.

关键词： liquefaction cone penetration test (CPT) standard penetration test (SPT) vibrcompaction sand

Evaluation of diverse classification approaches for land use/cover mapping in a Mediterranean region utilizing Hyperion data

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International Journal of Digital Earth 2014年第3期7卷 194-216页

作者： Alata Elatawneh Chariton Kalaitzidis George P.petropoulosc Thomas Schneider institute of Forest Management Technische Universität Muünchen(TUM)85354 FreisingGermany Department of Geoinformation in Environmental Management Mediterranean Agronomic Institute of Chania(MAICh)Chania 73100Greece INFOCOSMOS 13341 AthensGreece

Information on Earth’s land surface cover is commonly obtained through digital image analysis of data acquired from remote sensing *** this study,we evaluated the use of diverse classification techniques in discriminating land use/cover types in a typical Mediterranean setting using Hyperion *** this purpose,the spectral angle mapper(SAM),the object-based and the non-linear spectral unmixing based on artificial neural networks(ANNs)techniques were applied.A further objective had been to investigate the effect of two approaches for training sites selection in the SAM classification,namely of the pixel purity index(PPI)and of the direct selection of training points from the Hyperion imagery assisted by a QuickBird imagery and field-based training *** classification outperformed the other techniques with an overall accuracy of 83%.Sub-pixel classification based on the ANN showed an overall accuracy of 52%,very close to that of SAM(48%).SAM applied using the training sites selected directly from the Hyperion imagery supported by the QuickBird image and the field visits returned an increase accuracy by 16%.Yet,all techniques appeared to suffer from the relatively low spatial resolution of the Hyperion imagery,which affected the spectral separation among the land use/cover classes.

关键词： Hyperion Earth’s land use/cover mapping digital image analysis spectral angle mapper sub-pixel classification artificial neural networks Greece

Kinetics and thermodynamic studies of Methyl Orange removal by polyvinylidene fluoride-peDOT mats

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Journal of Environmental Sciences 2021年第2期33卷 62-73页

作者： Romario J.da Silva Lizeth Carolina Mojica-Sanchez Filipe D.S.Gorza Graciela C.pedro Bruna G.Maciel Gabriela P.Ratkovski Herica D.daRocha Kamila T.O.do Nascimento Juan CMedina-Llamas Alicia E.Chavez-Guajardo Jose J.Alcaraz-Espinoza Celso P.de Melo Program a de Pos-Graduagao em Ciencia de Materials U niversidade Federal de Pernambuco50670-901 RecifePEBrazil Departamento de Ffsica Uniuersidade Federal de Pemambiico50670-901 Reci/ePEBrazil Centro de Estudios Cientificosy Tecnologicos No.18 Instituto Politecnico Nacional98160 ZacatecasZacMexico Unidad Academica de Ciencias de la Tierra Uniuersidad Aut6noma de Zacatecas98058 ZacatecasZacMexico Departamento de Quimica Universidad Autonoma Metropolitana09340Ciudad de MexicoMexico

We report the preparation of poly(3,4-ethylene dioxythiophene)(peDOT)-modified polyvinylidene fluoride electrospun fibers and their use as a novel adsorbent material for the removal of the anionic dye Methyl Orange(MO)from aqueous *** novel adsorbent material can be used to selectively remove MO on a wide p H range(3.0-10.0),with a maximum capacity of 143.8 mg/g at p H *** used in a recirculating filtration system,the maximum absorption capacity was reached in a shorter time(20 min)than that observed for batch mode experiments(360 min).Based on the analyses of the kinetics and adsorption isotherm data,one can conclude that the predominant mechanism of interaction between the membrane and the dissolved dye molecules is ***,considering the estimated values for the Gibbs energy,and entropy and enthalpy changes,it was established that the adsorption process is spontaneous and occurs in an endothermic *** good mechanical and environmental stability of these membranes allowed their use in at least 20 consecutive adsorption/desorption cycles,without significant loss of their *** suggest that the physical-chemical characteristics of peDOT make these hybrid mats a promising adsorbent material for use in water remediation protocols and effluent treatment systems.

关键词： Electrospinning Composite mats pedot Pvdf mats Dye removal Water remediation

An unsettling explanation for the failure of skatole-baited ovitraps to capture Culex mosquitoes

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Insect Science 2019年第5期26卷 873-880页

作者： Marcelo H.S.Paiva Rosangela M.R.Barbosa Suzane A.Santos Norma M.Silva Marcia B.Paula Constancia F.J.Ayres Walter S.Leal Depariamento de Entomologia Instituto Aggeu MagalhaesFundagao Oswaldo CruzAv. Professor Moraes RegoRecifePEBrazil CentroAcademicodoAgreste Universidade Federalde pernambuco CaruaruPEBrazil Departamentode Biologia Celular Embriologia e GeneticaCentro de Ciencias Biologicas Universidade Federal de Santa CatarinaFlorianopolisSC.Brazil Faculdade de Saude PCiblica Departamento de Epidemiologia Universidade de Sao PauloSao PauloSPBrazil Department of Molecular and Cellular Biology University of California-DavisDavisCaliforniaUSA

Culex mosquitoes are primarily found in temperate and tropical regions worldwide where they play a crucial role as main vectors of filarial worms and arboviruses. In recife, a northeast city in Brazil, high densities of Culex quinquefasciatus are often found in association with human populated areas. In marked contrast to another part of the city, field tests conducted in the neighborhood of Sitio dos Pintos showed that trapping of mosquitoes in skatole-baited ovitraps did not differ significantly from captures in control (water) traps. Thus, classical and molecular taxonomic approaches were used to analyze the Culex species circulating in Sitio dos Pintos. Results obtained from both approaches agreed on the cocirculation of Culex quinquefasciatus and Culex nigripalpus in three different areas of this neighborhood. What was initially considered as an unexpected failure of this lure turned out to be a more unsettling problem, that is, the first report in recife of Culex nigripalpus, a vector of Venezuelan equine encephalitis virus and West Nile virus. Unplanned urbanization processes close to remnants of the Atlantic forest, such as observed in Sitio dos Pintos, may have contributed to the introduction of Cx. nigripalpus in urban areas.

关键词： Culex quinquefasciatus Culex nigripalpus dengue oviposition attractant skatole West Nile virus Zika

Production and Characterization of New Fibrinolytic Protease from Mucor subtillissimus UCP 1262 in Solid-State Fermentation

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Advances in Enzyme Research 2015年第3期3卷 81-91页

作者： Thiago Pajeu Nascimento Amanda Emmanuelle Sales Camila Souza Porto Romero Marcos pedrosa Brandao Galba Maria Campos Takaki Jose Antonio Couto Teixeira Tatiana Souza Porto Ana Lucia Figueiredo Porto Center for Biological Sciences Federal University of PernambucoRecifeBrazil Department of Morphology and Animal Physiology Federal Rural University of PernambucoRecifeBrazil Center for Research in Environmental Sciences Catholic University of PernambucoRecifeBrazil institute for Biotechnology and Bioengineering Centre of Biological EngineeringUniversity of MinhoCampus of GualtarBragaPortugal Academic Unit of Garanhuns Federal Rural University of PernambucoGaranhunsBrazil

Fibrinolytic enzymes have received attention regarding their medicinal potential for thrombolytic diseases, a leading cause of morbidity and mortality worldwide. Various natural enzymes purified from animal, plant and microbial sources have been extensively studied. The aim of this work was to produce fibrinolytic protease by solid state fermentation using agro industrial substrates. Rhizopus arrhizus var. arrhizus UCP 1295 and Mucor subtillissimus UCP 1262 filamentous fungi species isolated from soil of Caatinga-pe, Brasil, were used as producer microorganisms. Wheat bran was shown to be the best substrate for the production of the enzyme and by using a 23 full factorial design the main effects and interactions of the quantity of the substrate wheat bran, moisture and temperature on the fibrinolytic enzyme production and protease were evaluated. The best results for fibrinolytic and protease activities, 144.58 U/mL and 48.33 U/mL, respectively, were obtained with Mucor subtillissimus UCP 1262 using as culture medium 3 g wheat bran, 50% moisture at a temperature of 25°C for 72 hours. The optimum temperature for the produced enzyme was 45°C and most of its original activity was retained after being subjected to 80°C for 120 min. The protease activity was enhanced by K+, Ca+ and Mn+;but with Cu+ there was an inhibition. The specificity to chromogenic substrate and the inhibition by PMSF indicates that it is a chymotrypsin-like serine protease. Presented results suggest that this enzyme produced by solid-state fermentation is an interesting alternative as a candidate for thrombolytic therapy.

关键词： Mucor Enzyme Protease Fibrinolytic Wheat