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A rapid and sensitive liquid chromatography-tandem mass spectrometric assay for duloxetine in human plasma:Its pharmacokinetic application

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Journal of Pharmaceutical Analysis 2013年第1期3卷 36-44页

作者： Ramakrishna Gajula Rambabu Maddela Vasu Babu Ravi Jaswanth Kumar Inamadugu Nageswara Rao Pilli Wellquest Clinical research laboratories

This paper describes a simple, rapid and sensitive liquid chromatography tandem mass spectrometry assay for the determination of duloxetine in human plasma. A duloxetine stable labeled isotope （duloxetine ds） was used as an internal standard. Analyte and the internal standard were extracted from 100 btL of human plasma via solid phase extraction technique using Oasis HLB cartridges. The chromatographic separation was achieved on a Cl8 column by using a mixture of acetonitrile 5 mM ammonium acetate buffer （83：17, v/v） as the mobile phase at a flow rate of 0.9 mL/min. The calibration curve obtained was linear （r2≥0.99） over the concentration range of 0.05 101 ng/mL. Multiple-reaction monitoring mode （MRM） was used for quantification of ion transitions at rn/z 298.3/154.1 and 303.3/159.1 for the drug and the internal standard, respectively. Method validation was performed as per FDA guidelines and the results met the acceptance criteria. A run time of 2.5 min for each sample made it possible to analyze more than 300 plasma samples per day. The proposed method was found to be applicable to clinical studies.

关键词： Duloxetine in humanplasma Solid-phase extraction(SPE) Liquidchromatography-tandem mass spectro-metry Method validation Pharmacokinetic studies

Photonics-assisted THz wireless transmission with air interface user rate of 1-Tbps at 330–500 GHz band

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Science China(Information Sciences) 2023年第9期66卷 305-306页

作者： Min ZHU Jiao ZHANG Xiang LIU Bingchang HUA Yuancheng CAI Junjie DING Mingzheng LEI Yucong ZOU Liang TIAN Yunwu WANG Weidong TONG Jinbiao XIAO Yongming HUANG Jianjun YU Xiaohu YOU Purple Mountain laboratories National Mobile Communications research Laboratory Southeast University School of Information Science and technology Fudan University Peng Cheng Laboratory

Air interface user rate(AIUR) of 1-Tbps is one of the most important key performance indicators(KPIs) for the future6G extreme connectivity [1]. Terahertz(THz, 300 GHz–10 THz) is recognized as a promising candidate technology in achieving the KPI towards Tbps. In the past few years, a series of THz wireless transmissions at recordhigh data rates including both the pure-electronic and photonics-assisted solutions have been demonstrated [2].

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Counter-balancing effects of Si on C partitioning and stacking fault energy of austenite in 10Mn quenching and partitioning steel

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Journal of Materials Science & technology 2022年第3期98卷 248-257页

作者： Dong Hwi Kim Jee-Hyun Kang Hojun Gwon Joo Hyun Ryu Sung-Joon Kim Graduate Institute for Ferrous technology Pohang University of Science and TechnologyPohang 37673Republic of Korea School of Materials Science and Engineering Institute of Materials TechnologyYeungnam UniversityGyeongsan 38541Republic of Korea POSCO Technical research laboratories POSCOGwangyang 57807Republic of Korea

Silicon is an essential alloying element in quenching and partitioning(Q&P)steels,because it is known to suppress carbide precipitation during partitioning step and promote carbon partitioning to stabilize *** 2 wt%Si was added to 10Mn-2Al-0.2C steel,the size and fraction of the carbides formed during partitioning became smaller than in the Si-free ***,the suppression of carbide formation promoted C partitioning into austenite as ***,austenite stability was always lower with Si under the equivalent partitioning condition because Si effectively decreased the stacking fault energy of *** partitioning progressed,the both yield and tensile strengths of the Si-added steel exceeded that of the Si-free steel with the similar ductility *** was because Si was an effective solid solution strengthener,and the austenite in the Si-added steel exhibited the appropriate stability to gradually transform into martensite throughout the *** resulting strengthening effect compensated for the softening caused by martensite ***,strain hardening rate decreased continuously throughout deformation,which resulted in high tensile strength and ductility.

关键词： Steels Q&P steel Silicon Austenite stability Tensile deformation Stacking fault energy Medium Mn steel

Immunoassay of chemical contaminants in milk:A review

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Journal of Integrative Agriculture 2015年第11期14卷 2282-2295页

作者： XU Fei REN Kang YANG Yu-ze GUO Jiang-peng MA Guang-peng LIU Yi-ming LU Yong-qiang LI Xiu-bo National Feed Drug Reference laboratories Feed Research InstituteChinese Academy of Agricultural Sciences Beijing General Station of Animal Husbandry China Rural technology Development Center Ministry of Science and Technology of China

The detection of chemical contaminants is critical to ensure dairy safety. These contaminants include veterinary medicines, antibiotics, pesticides, heavy metals, mycotoxins, and persistent organic pollutants （POPs）. Immunoassays have recently been used to detect contaminants in milk because of their simple operation, high speed, and low cost. This article describes the latest developments in the most important component of immunoassays--antibodies, and then reviews the four major substrates used for immunoassays （i.e., microplates, membranes, gels, and chips） as well as their use in the detection of milk contaminants. The paper concludes with prospects for further aDDlications of these immunoassavs.

关键词： milk chemical contaminants immunoassay antibodies

In Vivo Toxicity Assessment of Gold Nanoparticles in Drosophila melanogaster

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Nano research 2011年第4期4卷 405-413页

作者： Pier Paolo Pompa Giuseppe Vecchio Antonio Galeone Virgilio Brunetti Stefania Sabella Gabriele Maiorano Andrea Falqui GiovanniBertoni Roberto Cingolani Italian Institute of technology Center for Bio-Molecular Nanotechnology Via Barsanti 1-73010 Arnesano （Lecce） Italy Italian Institute of technology Central Research Laboratories Via Morego 30-16136 Genova Italy

The growing use of nanomaterials in commercial goods and novel technologies is generating increasing questions about possible risks for human health and environment, due to the lack of an in-depth assessment of their potential toxicity. In this context, we investigated the effects of citrate-capped gold nanoparticles （AuNPs） on the model system Drosophila melanogaster upon ingestion. We observed a significant in vivo toxicity of AuNPs, which elicited clear adverse effects in treated organisms, such as a strong reduction of their life span and fertility, presence of DNA fragmentation, as well as a significant overexpression of the stress proteins. Transmission electron microscopy demonstrated the localization of the nanoparticles in tissues of Drosophila. The experimental evidence of high in vivo toxicity of a nanoscale material, which is widely considered to be safe and biocompatible in its bulk form, opens up important questions in many fields, including nanomedicine, material science, health, drug delivery and risk assessment.

关键词： Nanoparticles nanotoxicology gold in vivo studies

advanced HSLA Steels for Automotive Use

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Journal of Iron and Steel research International 2011年第S1期18卷 49-56页

作者： Sekita Takashi Seto Kazhiro Funakawa Yoshimasa Steel research laboratories JFE Steel Corporation260-0835ChibaJapan Steel research laboratories JFE Steel Corporation210-0855KawasakiJapan

Characteristic HSLA steels for automotive use are introduced in which both of precipitation and microstructure is controlled to obtain suitable mechanical *** outer panels such as fender,the combination of low yield strength,high tensile strength and deep-drawability were realized by controlling the distribution of NbC and precipitation free *** other steel,developed for chassis parts such as lower arm,utilizes extremely fine interphase precipitation to obtain high yield strength and excellent hole *** steels have contributed to the reduction of weight in car body.

关键词： precipitation free zone (PFZ) deep drawability interphase precipitation hole expansionability yield strength

Effect of Boron Concentration on Dynamic Recrystallization Behavior of Low-Carbon Steel

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Acta Metallurgica Sinica(English Letters) 2015年第7期28卷 931-939页

作者： Yong-Liang Gao Xiang-Xin Xue He Yang Liaoning Key laboratories of Ecological Utilization technology of Boron Resources and Boron Materials Northeastern University

The dynamic recrystallization behavior of low-carbon steel containing three different amounts of boron（0.002,0.004,and 0.006 wt%） was systematically investigated under various experimental ***-carbon steel was exposed to temperatures ranging from 900 to 1100 ℃,strain rates from 0.1 to 10 s^-1,and inspection of the initial austenite grain size at 1150℃.The resulting stress–strain curves are observed to possess two classifications of behavior,dynamic recovery and dynamic recrystallization,while the initial austenite grain size increases directly proportional to boron ***,the characteristic points of the flow curves were analyzed by regression method in which the peak and critical stresses decreased in response to an increase in boron composition,indicating that a softening effect appears with the addition of *** the contrary,peak and critical strains increased as boron content increased,indicating that boron has the ability to delay the onset of dynamic ***,the kinetics model of dynamic recrystallization for three boron-treated steels was established.

关键词： Boron-treated steel Hot deformation Flow stress Dynamic recrystallization

My Journey to DNA Repair

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Genomics, Proteomics & Bioinformatics 2013年第1期11卷 2-7页

作者： Tomas Lindahl Cancer research UK London research Institute Clare Hall Laboratories

I completed my medical studies at the Karolinska Institute in Stockholm but have always been devoted to basic research. My longstanding interest is to understand fundamental DNA repair mechanisms in the fields of cancer therapy, inherited human genetic disorders and ancient DNA. I initially measured DNA decay, including rates of base loss and cytosine deamination. I have dis- covered several important DNA repair proteins and determined their mechanisms of action. The discovery of uracil-DNA glycosylase defined a new category of repair enzymes with each specialized for different types of DNA damage. The base excision repair pathway was first reconstituted with human proteins in my group. Cell-free analysis for mammalian nucleotide excision repair of DNA was also developed in my laboratory. I found multiple distinct DNA ligases in mammalian cells, and led the first genetic and biochemical work on DNA ligases I, III and IV. I discovered the mam- malian exonucleases DNase III （TREX1） and IV （FEN1）. Interestingly, expression of TREXI was altered in some human autoimmune diseases. I also showed that the mutagenic DNA adduct O6-methylguanine （O6mG） is repaired without removing the guanine from DNA, identifying a sur- prising mechanism by which the methyl group is transferred to a residue in the repair protein itself. A further novel process of DNA repair discovered by my research group is the action of AlkB as an iron-dependent enzyme carrying out oxidative demethylation.

关键词： DNA repair Base excision repair DNA glycosylase DNA exonuclease AlkB dioxygenase

Corrosion behavior of ultrafine-grained AA2024 aluminum alloy produced by cryorolling

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International Journal of Minerals,Metallurgy and Materials 2017年第11期24卷 1293-1305页

作者： P. Laxman Mani Kanta V.C. Srivastava K. Venkateswarlu Sharma Paswan B. Mahato Goutam Das K. Sivaprasad K. Gopala Krishna Department of Metallurgical & Materials Engineering National Institute of Technology Thiruchirappalli 620015 India National Metallurgical Laboratory Council of Scientific & Industrial Research Jamshedpur 831007 India National Aerospace laboratories Council of Scientific & Industrial Research Bangalore 560017 India

The objectives of this study were to produce ultrafine-grained(UFG) AA2024 aluminum alloy by cryorolling followed by aging and to evaluate its corrosion behavior. Solutionized samples were cryorolled to ~85% reduction in thickness. Subsequent aging resulted in a UFG structure with finer precipitates of Al_2CuMg in the cryorolled alloy. The(1) solutionized and(2) solutionized and cryorolled samples were uniformly aged at 160°C/24 h and were designated as CGPA and CRPA, respectively; these samples were subsequently subjected to corrosion studies. Potentiodynamic polarization studies in 3.5 wt% NaCl solution indicated an increase in corrosion potential and a decrease in corrosion current density for CRPA compared to CGPA. In the case of CRPA, electrochemical impedance spectroscopic studies indicated the presence of two complex passive oxide layers with a higher charge transfer resistance and lower mass loss during intergranular corrosion tests. The improved corrosion resistance of CRPA was mainly attributed to its UFG structure, uniform distribution of fine precipitates, and absence of coarse grain-boundary precipitation and associated precipitate-free zones as compared with the CGPA alloy.

关键词： AA2024 alloy cryorolling ultrafine grains precipitation corrosion behavior