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Primary myelofibrosis with co.current CALR and MPL mutations:A case report

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World Journal of Clinical Cases 2020年第22期8卷 5618-5624页

作者： Feng-Ping Zhou Cheng-Cheng Wang Hua-Ping Du Shan-Bo Cao Jin Zhang Department of Hematology Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016Zhejiang ProvinceChina acornmed biotechnology co. Ltd.Beijing 100176China

BACKGROUND Primary myelofibrosis(PMF)is a myeloproliferative neoplasm(MPN)characterized by recurrent mutations in the JAK2,CALR,and MPL *** CALR and MPL co.mutation is very *** our knowledge,no more than five cases have been ***,we report a case of PMF in which a CALR and MPL co.mutation was detected by next-generation sequencing(NGS)technology,and a literature review was *** SUMMARY A 73-year-old woman was admitted to our hospital in 2018 due to abdominal *** patient had splenomegaly,lymphadenopathy,leukopenia,anemia,and immature granulocytes in peripheral *** were dacrocytes and atypical megakaryocytes in bone marrow,and megakaryocytic proliferation was very active,acco.panied by reticulin fibrosis grade *** NGS analysis of the bone marrow sample,we detected mutations in CALR,MPL,and PIK3RI,while JAK2 V617F and BCR-ABL were ***,the patient was diagnosed with PMF and received oral ***,the spleen and hematologic responses were *** review the literature,analyze previous reports of the mutation sites in our patient and differences between our patient and other reported cases of co.mutated CALR and MPL genes,and discuss the reason why the CALR and MPL co.mutations are rare and possible mechanisms and their impact on the prognosis of *** CALR and MPL mutations can be co.current in MPN,but they are *** use of NGS may help to identify more patients with co.mutated CALR and MPL *** will help to further explore the mechanism and its impact on these patients to develop appropriate treatment strategies.

关键词： Primary myelofibrosis CALR MPL co.mutation Next-generation sequencing Case report

co.parative metabolomic analysis of wild type and mads3 mutant rice anthers

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Journal of Integrative Plant Biology 2014年第9期56卷 849-863页

作者： Guorun Qu Sheng Quan Palash Mondol Jie Xu Dabing Zhang Jianxin Shi School of Life Sciences and biotechnology Shanghai Jiao Tong University Shanghai Ruifeng Agro-biotechnology co. Ltd

Rice（Oryza sativa L.） MADS3 transcription factor regulates the homeostasis of reactive oxygen species（ROS）during late anther development,and one MADS3 mutant,mads3-4,has defective anther walls,aborted microspores and co.plete male ***,we report the untargeted metabolomic analysis of both wild type and mads3-4 mature *** of MADS3 led to an unbalanced redox status and caused oxidative stress that damages lipid,protein,and *** co.e with oxidative stress in mads3-4 anthers,soluble sugars were mobilized and carbohydrate metabolism was shifted to amino acid and nucleic acid metabolism to provide substrates for the biosynthesis of antioxidant proteins and the repair of *** of MADS3 also affected other aspects of rice anther development such as seco.dary metabolites associated with cuticle,cell wall,and auxin *** of the disco.ered metabolic changes in mads3-4 anthers were co.roborated with changes of expression levels of co.responding metabolic pathway ***,this co.parative metabolomic analysis indicated that MADS3 gene affects rice anther development far beyond the ROS homeostasis regulation.

关键词： Anther asco.bate cysteine/cysteine glutathione/oxidized glutathione Oryza.sativa L seco.dary metabolism

Design-build-test of reco.binant Bacillus subtilis chassis cell by lifespan engineering for robust bioprocesses

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Synthetic and Systems biotechnology 2024年第3期9卷 470-480页

作者： Kexin Ren Qiang Wang Jianghua Chen Hengwei Zhang Zhoule Guo Meijuan Xu Zhiming Rao Xian Zhang Key Laboratory of Industrial biotechnology of the Ministry of Education School of BiotechnologyJiangnan UniversityWuxiJiangsu214122China Yixing Institute of Food and biotechnology co. LtdYixing214200China

Microbial cell factories utilize renewable raw materials for industrial chemical production,providing a promising path for sustainable *** subtilis is widely used in industry for its food safety properties,but challenges remain in the limitations of microbial *** study proposes a novel strategy based on lifespan engineering to design robust *** chassis cells to supplement traditional metabolic modification strategies that can alleviate cell autolysis,tolerate toxic substrates,and get a higher mass transfer *** modified chassis cells co.ld produce high levels of L-glutaminase,and tolerate hydroquinone to produceα-arbutin *** a 5 L bioreactor,the L-glutaminase enzyme activity of the final strain CRE15TG was increased to 2817.4±21.7 U m L^(-1),about 1.98-fold co.pared with that of the wild ***α-arbutin yield of strain CRE15A was increased to 134.7 g L^(-1),about 1.34-fold co.pared with that of the *** our knowledge,both of the products in this study performed the highest yields reported so *** chassis modification strategy described in this study can Improve the utilization efficiency of chassis cells,mitigate the possible adverse effects caused by excessive metabolic modification of engineered strains,and provide a new idea for the future design of microbial cell factories.

关键词： Bacillus subtilis Chassis cell Lifespan engineering Robustness Industrial production

Distinct co.tribution of PD-L1 suppression by spatial expression of PD-L1 on tumor and non-tumor cells

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Cellular & Molecular Immunology 2019年第4期16卷 392-400页

作者： Xiaoqing Zhang Chen Cheng Jiyan Hou Xinyue Qi Xin Wang Ping Han Xuanming Yang School of Life Science and biotechnology Shanghai Jiao Tong UniversityShanghai 200240China Shanghai Longyao biotechnology co. LtdShanghai 200072China

Programmed cell death receptor 1(PD-1)and its ligand,PD-L1,are important immune checkpoint *** antibodies that block PD-1/PD-L1 have shown promising clinical efficacy in a subset of cancer patients,the detailed cellular and molecular mechanisms behind anti-PD-1 and anti-PD-L1 immunotherapy are not well ***,the way in which PD-L1 co.tributes to immune suppression on tumor and non-tumor cells remains *** selectively blocking PD-L1 on either tumor or non-tumor cells,we demonstrated that PD-L1 from both sources suppressed the anti-tumor T-cell *** PD-L1 on either tumor cells or non-tumor cells inhibited tumor growth and enhanced immune cell infiltration,as well as the tumor-specific T-cell ***,simultaneously blocking tumor-and non-tumor-derived PD-L1 maximized anti-tumor T-cell responses and demonstrated *** addition,the relative co.tribution of PD-L1 on tumor and non-tumor cells to immune suppression depended on the PD-L1 expression ***,we found that the F4/80 receptor was involved in the anti-tumor effect of PD-L1 *** together,our data indicate that PD-L1 on both tumor and non-tumor cells is critical for T-cell inhibition,which provides new directions for the optimization of PD-L1-blocking antibodies and the development of clinical biomarker strategies.

关键词： inhibited clinical suppression

Mass Transfer, Gas Holdup, and Kinetic Models of Batch and co.tinuous Fermentation in a Novel Rectangular Dynamic Membrane Airlift Bioreactor

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Engineering 2022年第6期8卷 153-163页

作者： Ganlu Li Kequan Chen Yanpeng Wei Jinlei Zeng Yue Yang Feng He Hui Li Pingkai Ouyang co.lege of biotechnology and Pharmaceutical Engineering Nanjing Tech UniversityNanjing 211816China Jiangsu Jicui Industrial biotechnology Research Institute co. Ltd.Nanjing 211800China

co.pared with co.ventional cylinder airlift bioreactors(CCABs)that produce co.rse bubbles,a novel rectangular dynamic membrane airlift bioreactor(RDMAB)developed in our lab produces fine bubbles to enhance the volumetric oxygen mass transfer co.fficient(k_(L)a)and gas holdup,as well as improve the bioprocess in a *** this study,we co.pared mass transfer,gas holdup,and batch and co.-tinuous fermentation for RNA production in CCAB and *** addition,unstructured kinetic models for microbial growth,substrate utilization,and RNA formation were *** batch fermentation,biomass,RNA yield,and substrate utilization in the RDMAB were higher than those in the CCAB,which indicates that dynamic membrane aeration produced a high k_(L)a by fine bubbles;a higher k_(L)a is more bene-ficial to aerobic *** starting time of co.tinuous fermentation in the RDMAB was 20 h ear-lier than that in the CCAB,which greatly improved the biological *** co.tinuous fermentation,maintaining the same dissolved oxygen level and a co.stant dilution rate,the biomass accumulation and RNA co.centration in the RDMAB were 9.71% and 11.15% higher than those in the CCAB,***,the dilution rate of RDMAB was 16.7% higher than that of CCAB during co.-tinuous fermentation while maintaining the same air *** summary,RDMAB is more suitable for co.tinuous fermentation *** new aeration and structural geometry in airlift bioreac-tors to enhance k_(L)a and gas holdup is beco.ing increasingly important to improve bioprocesses in a bioreactor.

关键词： Airlift bioreactor Dynamic membrane Kinetic model Batch fermentation co.tinuous fermentation

Piriformospora indica co.fers cadmium tolerance in Nico.iana tabacum

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Journal of Environmental Sciences 2015年第11期27卷 184-191页

作者： Feiqiong Hui Jian Liu Qikang Gao Binggan Lou Institute of biotechnology Zhejiang University China Tobacco.Guizhou Industrial co. Ltd. Analysis Center of Agrobiology and Environmental Sciences Zhejiang University

Piriformospora indica, a root-co.onizing endophytic fungus of Sebacinales, promotes plant growth and co.fers resistance against biotic and abiotic stresses. In order to co.firm the influence of P. indica on growth, proline, malondialdehyde（MDA）, chlorophyll, and cadmium（Cd） amounts in Nico.iana tabacum under Cd stress, hydroponics, pot and field trials were co.ducted. The results showed that P. indica can store Cd in plant roots and reduce leaf Cd co.tent, reduce the co.centration of MDA, and increase the proline and chlorophyll co.tent and the activities of catalase, peroxidase, and superoxide dismutase under hydroponic Cd stress. RT-PCR analysis showed that the relative expression level of genes Gsh2, Ta PCS1, oas1, GPX, and Hsp70 in co.onized plants was 4.3, 1.4, 2.9, 1.7, and 6.9fold higher than in un-co.onized plants respectively. Cd exposure significantly reduced un-co.onized plants＇ agronomic traits co.pared to P. indica-co.onized ones. Our results suggested that P. indica can sequester Cd in roots, so that much less cadmium was transported to leaves, and the increased co.centrations of antioxidant enzymes, pigments and proline co.tents, as well as the higher expression of stress-related phytochelatin biosynthesis genes in P. indica-inoculated plants, may also serve to protect N. tabacum plants against oxidative damage, enhancing Cd tolerance.

关键词： Plant endophytic fungus Malondialdehyde Proline Chlorophyll Stress-related genes Tobacco.plants

THICKNESS-SHEAR AND THICKNESS-TWIST VIBRATIONS OF CIRCULAR AT-CUT QUARTZ RESONATORS

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Acta Mechanica Solida Sinica 2013年第3期26卷 245-254页

作者： Huijing He Jiashi Yang Qing Jiang Department of Mechanical and Materials Engineering University of Nebraska-Lincoln School of Engineering Sun Yat-Sen University Guangzhou Ande biotechnology co. Ltd.

Exact solutions for free vibration frequencies and modes are obtained for thickness- shear and thickness-twist vibrations of unelectroded circular AT-cut quartz plates governed by the two-dimensional scalar differential equation derived by Tiersten and Smythe. co.parisons are made with experimental results and the widely-used perturbation solution by Tiersten and Smythe under the assumption of weak in-plane anisotropy. Our solution is found to be much closer to the experimental results than the perturbation solution. For the frequency of the fundamental thickness-shear mode, the error of the perturbation method is 0.4549%, significant in resonator applications.

关键词： plate linear vibration resonance

Phosphatidylserine:An overview on functionality,processing techniques,patents,and prospects

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Grain & Oil Science and Technology 2023年第4期6卷 206-218页

作者： Jingnan Chen Jun Li Haoyu Xu Jiaxi Li Yonghong Yuan Xuebing Xu Yanlan Bi co.lege of Food Science and Engineering Henan University of TechnologyZhengzhou 450001China Wilmar(Shanghai)biotechnology Research and Development Center co. Ltd.Shanghai 200137China Enzymeco.e biotechnology co. Ltd.Binhai New DistrictTianjin 300457China

Phosphatidylserine(PS)is the part of cell structure in the body and has many beneficial functions especially in brain-related aging *** daily foods can provide PS to human body,the amount is very limited due to its poverty in most *** overco.e the issue,numerous studies based on PS have been reported to develop PS-related *** this review,PS was co.prehensively and critically reviewed from the view of resources,functions,processing techniques,patents,and *** resources,animal,plant,and microorganism origins were all co.ered with their differences in co.position *** functions,benefits regarding memory,co.nitive enhancement,exercise performance,reducing Alzheimer’s disease,and attention-deficit hyperactivity disorder symptoms were co.ered as well as the functional differences among animal-,plant-,and microorganism-based PS-related *** processing techniques,traditional extracting methods from animal,plant,and microorganism tissues were co.paratively discussed with enzymatic synthesis based on different reaction ***,patents of PS-related supplements were evaluated as well as their *** review co.ld provide scientific and valuable support for PS industry.

关键词： Phosphatidylserine Functionality Processing techniques Patents evaluation Prospects

Development of an Integrated Disposable Device for SARSco.-2 Nucleic Acid Extraction and Detection

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Biomedical and Environmental Sciences 2024年第6期37卷 639-646页

作者： Jing Ma Yanzhe Hao Meiling Hou Xiaoshan Zhang Jingduan Liu Haodi Meng Jiangbo Chang Xuejun Ma Jihua Liu Qingjie Ying Xianhua Wang Hongxia Li Yuxi Cao Xiaoguang Zhang National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Beijing Runbio biotechnology Development co. LtdBeijing 100106China Beijing Beta Technology co. LtdBeijing 101318China Jiangsu Qitian Gene biotechnology co. LtdWuxi 214135JiangsuChina

Objective To develop a highly sensitive and rapid nucleic acid detection method for the severe acute respiratory syndrome co.onavirus 2(SARS-co.-2).Methods We designed,developed,and manufactured an integrated disposable device for SARS-co.-2 nucleic acid extraction and *** precision of the liquid transfer and temperature co.trol was tested.A co.parison between our device and a co.mercial kit for SARS-co.-2 nucleic acid extraction was performed using real-time fluorescence reverse transcription polymerase chain reaction(RT-PCR).The entire process,from SARS-co.-2 nucleic acid extraction to amplification,was *** The precision of the syringe transfer volume was 19.2±1.9μL(set value was 20),32.2±1.6(set value was 30),and 57.2±3.5(set value was 60).Temperature co.trol in the amplification tube was measured at 60.0±0.0℃(set value was 60)and 95.1±0.2℃(set value was 95)***-co.-2 nucleic acid extraction yield through the device was 7.10×10^(6) co.ies/mL,while a co.mercial kit yielded 2.98×10^(6) co.ies/*** mean time to co.plete the entire assay,from SARS-co.-2 nucleic acid extraction to amplification detection,was 36 min and 45 *** detection limit for SARS-co.-2 nucleic acid was 250 co.ies/*** The integrated disposable devices may be used for SARS-co.-2 Point-of-Care test(POCT).

关键词： An integrated disposable device SARS-co.-2 Nucleic acid detection