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Cobalamin-dependent radical S-adenosyl-L-methionine protein functions with a partner to successively methylate tricyclic indole alkaloid for chuangxinmycin maturation and derivatization

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Science China Chemistry 2025年第1期68卷 308-316页

作者： Sili Wang Jiancheng Huang Yanan Du Wei Huang Yufeng Xue Xiaokun Xu Guannan Zhong Yuanyuan Shi Bin Hong Xiaoying Bian Wen Liu State Key Laboratory of Chemical biology Shanghai Institute of Organic ChemistryUniversity of Chinese Academy of SciencesChinese Academy of SciencesShanghai 200032China State Key Laboratory of Microbial Technology Shandong UniversityQingdao 266237China National Health Commission(NHC)Key Laboratory of biotechnology for Microbial Drugs Institute of Medicinal BiotechnologyChinese Academy of Medical Science&Peking Union Medical CollegeBeijing 100050China

Cobalamin(Cbl)-dependent radical S-adenosyl-L-methionine(SAM)proteins constitute the largest collection of the radical SAM superfamily that has hundreds of thousands of individual *** of these proteins are involved in the biosynthesis of pharmaceutically important natural products to catalyze chemically demanding *** the biosynthetic pathway of chuangxinmycin(CXM),a unique indole alkaloid antibiotic with potent anti-infective activity,functionalization of the characteristic thiopyrano[4,3,2-cd]indole scaffold by regio-and stereoselective C3-methylation is believed to rely on a Cbl-dependent radical process,which,however,remained to be reconstituted *** here report the dissection of this enzymatic process,which requires the incorporation of Cxm8,a Cbl-dependent radical SAM protein,with Cxm9,a DUF5825family protein that shares no homology to any proteins of known ***8 and Cxm9 function together by forming an unexpected heterodimeric complex that selectively catalyzes C3-methylation of the tricyclic indole-S-hetero ring system in a successive manner,achieving CXM and a recently identified,C3-dimethylated *** biochemical characterization,isotope labeling,structural simulation and bioinformatics analysis rationalized the catalysis of the Cxm8/Cxm9 complex and particularly the necessity of the DUF5825 protein for C3-methylase *** is the first example that a Cbl-dependent protein acts with a partner to exhibit radical SAM activity.

关键词： cbl-dependent radical SAM protein biosynthesis chuangxinmycin stereoselective methylation

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Response surface optimization of preparation parameters and evaluation of the antioxidant and antihypertensive activities of Spirulina protein hydrolysates

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Journal of Oceanology and Limnology 2025年第1期43卷 196-209页

作者： Qishan SUO Zhenzhen DENG Yang YUE Jing WANG Ning WU Lihua GENG Quanbin ZHANG CAS and Shandong Province Key Laboratory of Experimental Marine biology Center for Ocean Mega-ScienceInstitute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for Marine biology and biotechnology Qingdao Marine Science and Technology CenterQingdao 266237China University of Chinese academy of Sciences Beijing 101408China

Safety,high protein content,and the numerous health benefits make Spirulina an outstanding source of bioactive *** from Spirulina,which exhibit antioxidant,anti-inflammatory,and antihypertensive effects,may assist in the management of cardiovascular diseases(CVDs).Here,we present the optimization of enzyme-digested hydrolysates derived from Spirulina *** using response surface methodology(RSM).We also evaluated the biological activity of Spirulina protein hydrolysates(SPHs)in contributing to the amelioration of hypertension and associated cardiovascular *** findings suggest that Spirulina protein extracts contain a complex proteinaceous composition with phycocyanin being the dominant protein,as evidenced by both SDS-PAGE and LC-MS/MS *** protease,protease K,andα-chymotrypsin exhibited a substantial hydrolytic effect on Spirulina protein,taking into account their degree of hydrolysis(DH),peptide yield,and molecular weight ***-factor experiments identified pH,temperature,and enzyme/substrate ratio as the key factors influencing ***,optimal hydrolysis conditions for the proteases were determined via RSM,leading to a considerable enhancement of the actual *** showed that SPHs have strong antioxidant activity against various free radicals,with a higher ability to scavenge hydroxyl ***,SPHs inhibited macrophage nitric oxide secretion and significantly inhibited angiotensin I-converting enzyme in vitro at 400μg/*** is worth noting that SPHs prepared with protease K exhibited a potent anti-hypertensive effect in *** findings indicate the potential usefulness of including dietary SPHs in preventing hypertension and associated *** research could be valuable in guiding the development of health-promoting foods within the Spirulina industry.

关键词： antioxidant activity antihypertensive effect angiotensin converting enzyme inhibition response surface methodology Spirulina

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Morphology and phylogeny of two species of Bathypathes from the tropical western Pacific,with description of a new species

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Journal of Oceanology and Limnology 2025年第1期43卷 248-260页

作者： Ting LÜ Zifeng ZHAN Kuidong XU Laboratory of Marine Organism Taxonomy and Phylogeny Qingdao Key Laboratory of Marine Biodiversity and ConservationInstitute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for Marine biology and biotechnology Laoshan LaboratoryQingdao 266237China University of Chinese academy of Sciences Beijing 100049China

The black coral genus Bathypathes Brook,1889 represents a relatively diverse and ecologically important assemblage comprising the deep-sea hard substrate *** phylogenetic investigations have revealed the polyphyletic nature of Bathypathes,urging further morphological analysis to understand its *** combing morphological and molecular data,we describe Bathypathes multifurcata *** report *** Molodtsova,Opresko&Wagner,2022based on the specimens collected from the tropical western *** phylogenetic topology reconstructed using nuclear ITS1-5.8S-ITS2 and three mitochondrial fragments suggests the separation of Bathypathes into four *** analysis of morphological characters,particularly the colony branch and pinnule patterns also roughly classify Bathypathes into four morphological groups A–*** A is characterized by the unbranched colony and the subopposite arrangement of *** contrast to Group A,Groups B–D have the alternately arranged *** these,Group C exhibits a distinctly long unpinnulated stem compared to Groups B and *** B has unbranched colony,relatively short unpinnulated stem,and spines on pinnules have blunt *** Group D,large colonies(higher than 40 cm)have one or a few branches,small colonies(less than 40 cm)are unbranched,and spines on pinnules have acute *** data particularly those species without sequences are necessary to verify the classification of these groups.

关键词： Antipatharia black coral taxonomy phylogeny seamount western Pacific

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Eukaryotic translation initiation factor OseIF5A4 negatively regulates rice immunity against blast and bacterial blight diseases

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Crop Journal 2025年第1期13卷 79-91页

作者： Wang, Hui Yan, Yuqing Bi, Yan Li, Dayong Song, Fengming National Key Laboratory for Rice biology and Breeding Institute of Biotechnology Zhejiang University Zhejiang Hangzhou 310058 China Ministry of Agriculture Key Laboratory of Molecular biology of Crop Pathogens and Insect Pests Institute of Biotechnology Zhejiang University Zhejiang Hangzhou 310058 China Key Laboratory of biology of Crop Pathogens and Insects of Zhejiang Province Institute of Biotechnology Zhejiang University Zhejiang Hangzhou 310058 China

The RING-type E3 ligase OsBBI1 regulates rice resistance against Magnaporthe oryzae through modifying cell wall defenses. In this study, we report the function of an OsBBI1 substrate, eukaryotic translation initiation factor OseIF5A4, in rice immunity. OsBBI1 interacts with OseIF5A4 and other four members of the OseIF5A family. The RING domain in OsBBI1 and the eIF-5a domain in OseIF5A4 are critical for the OsBBI1-OseIF5A4 interaction. OsBBI1 ubiquitinates OseIF5A4 and mediates its degradation in vitro and in vivo. Moreover, the expression of OseIF5A4 was upregulated during early stage of compatible interaction but downregulated in incompatible interaction between rice and M. oryzae. Knockout of OseIF5A4 enhances rice immunity against M. oryzae and Xanthomonas oryzae pv. oryzae, boosts pattern-triggered immune responses, and strengthens pathogen-induced defense responses (e.g., expression of defense genes, accumulation of reactive oxygen species and reinforcement of cell wall). However, overexpression of OseIF5A4 attenuates rice immunity and immune responses. These results demonstrate that OseIF5A4, a substrate of the immunity-associated E3 ligase OsBBI1, negatively regulates rice immunity against M. oryzae and X. oryzae pv. oryzae through modulating pathogen-induced defense responses, highlighting the importance of the protein translational machinery in rice immunity. © 2025 Crop Science Society of China and Institute of Crop Science, CAAS

关键词： Magnaporthe oryzae OsBBI1 OseIF5A Rice immunity Xanthomonas oryzae pv. oryzae

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Artificial intelligence advances drug delivery system and its clinical transition

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Science Bulletin 2025年第3期70卷 305-308页

作者： Hui Wang Xiao-Yan You Guo-Ping Zhao Master Lab for Innovative Application of Nature Products National Center of Technology Innovation for Synthetic BiologyTianjin Institute of Industrial BiotechnologyChinese Academy of Sciences(CAS)Tianjin 300308China Henan Engineering Research Center of Food Microbiology College of Food and BioengineeringHenan University of Science and TechnologyLuoyang 471023China Haihe Laboratory of Synthetic biology Tianjin 300308China CAS-Key Laboratory of Synthetic biology CAS Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China CAS Key Laboratory of Quantitative Engineering biology Shenzhen Institute of Synthetic BiologyShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China

Since November 2022,ChatGPT,an artificial intelligence(AI)chatbot,has rapidly captured global attention for its detailed responses and articulated answers across a broad spectrum of knowledge domains[1],[2].All industries will reorient around it,prompting a reevaluation of how this technology could further promote scientifical research and its clinical applications.

关键词： system transition spectrum

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Mixture of Bacillus Amyloliquefaciens and Bacillus Pumilus Modulates Community Structures of Rice Rhizosphere Soil to Suppress Rice Seedling Blight

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Rice science 2025年第1期32卷 118-130,I0067-I0070页

作者： JIANG Nan QIU Jiehua TIAN Dagang SHI Huanbin LIU Zhiquan WEN Hui XIE Shuwei CHEN Huizhe WU Meng KOU Yanjun State Key Laboratory of Rice biology and Breeding China National Rice Research InstituteHangzhou 311400China biotechnology Research Institute Fujian Academy of Agricultural SciencesFuzhou 350003China State Key Laboratory of Soil and Sustainable Agriculture the Institute of Soil ScienceChinese Academy of SciencesNanjing 210008China

Rice seedling blight,caused by various fungi,including Fusarium oxysporum,poses a severe threat to rice *** awareness grows regarding the environmental and safety hazards associated with the application of fungicides for managing rice seedling blight,there has been a shift in focus towards biological control *** this study,we isolated biocontrol bacteria from paddy fields that significantly inhibited the growth of *** in vitro and identified the strains as Bacillus amyloliquefaciens T40 and Bacillus pumilus ***,our findings indicated that the combined application of these Bacillus strains in soil was more effective in reducing the incidence of rice seedling blight than their individual *** of 16S and internal transcribed spacer rRNA gene sequencing data revealed that the mixture of the T40 and T208 strains exhibited the lowest average clustering coefficients,which were negatively correlated with the biomass of ***-inoculated rice ***,this mixture led to higher stochastic assembly(average|βNTI|<2)and reduced selection pressures on rice rhizosphere bacteria compared with individual strain *** mixture of the T40 and T208 strains also significantly increased the expression of defense-related *** conclusion,the mixture of the T40 and T208 strains effectively modulates microbial community structures,enhances microbial network stability,and boosts the resistance against rice seedling *** study supports the development and utilization of biological resources for crop protection.

关键词： application strategy disease control disease resistance microbial community structure microbial community assembly process Oryza sativa

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A C-type lectin with dual carbohydrate recognition domains functions in innate immune response in Asian corn borer,Ostrinia furnacalis

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Insect Science 2025年第1期32卷 172-192页

作者： Er-Tao Li Jia-Yue Ji Wei-Jie Kong Dong-Xu Shen Cai Li Chun-Ju An Deparment of Entomology College of Plant ProtectionChina Agricultural UniversityBejingChina Pomology Institute Shanxi Agricultural UniversityJinzhongShanxi ProvinceChina Jiangsu Key Laboratory of Sericultural biology and biotechnology School of BiotechnologyJiangsu University of Science and TechnologyZhenjiangJiangsu ProvinceChina

C-type lectins(CTLs)act as pattern recognition receptors(PRRs)to initiate the innate immune response in insects.A CTL with dual carbohydrate recognition domains(CRDs)(named immulectin-4[IML-4])was selected from the Ostrinia furnacalis transcriptome dataset for functional *** cloned the full-length complementary DNA of *** IML-4(OfIML-4).It encodes a 328-residue protein with a Glu-Pro-Asn(EPN)and Gln-Pro-Asp(QPD)motifs in 2 CRDs,***-4 messenger RNA levels increased significantly upon the bacterial and fungal *** OfIML-4(rIML-4)and its individual CRDs(rCRD1 and rCRD2)exhibited the binding ability to various microorganisms including Escherichia coli,Micrococcus luteus,Pichia pastoris,and Beauveria bassiana,and the cell wall components including lipopolysaccharide from ***,peptidoglycan from *** or Bacillus subtilis,and curdlan from Alcaligenes *** binding further induced the agglutination of ***,***,and *** in the presence of calcium,the phagocytosis of Staphylococcus aureus by the hemocytes,in vitro encapsulation and melanization of nickel-nitrilotriacetic acid beads,and a significant increase in phenoloxidase activity of *** addition,rIML-4 significantly enhanced the phagocytosis,nodulation,and resistance of *** to *** together,our results suggest that OfIML-4 potentially works as a PRR to recognize the invading microorganisms,and functions in the innate immune response in ***.

关键词： cellular immunity C-type lectin humoral immunity immulectin Ostrinia furnacalis pattern recognition receptor(PRR)

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Deciphering fungal metabolon coupling tandem inverse-electrondemand Diels-Alder reaction and semipinacol rearrangement for the biosynthesis of spiro polycyclic alkaloids

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Science China Chemistry 2025年第1期68卷 288-296页

作者： Shuai Liu Wen-Qiang Xu Ying-Tong Di Man-Cheng Tang Ding-Kang Chen Ming-Ming Cao Yao-Wen Chang Hong-Yu Tang Chun-Mao Yuan Jun-Bo Yang Zhi-Li Zuo Han Guo Zi-Fei Xu Ying Zeng Yun-Dong Wu Xiao-Jiang Hao Key Laboratory of Phytochemistry and Natural Medicines(Chinese academy of Sciences) Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China Lab of Computational Chemistry and Drug Design State Key Laboratory of Chemical OncogenomicsPeking University Shenzhen Graduate SchoolShenzhen 518055China Shenzhen Bay Laboratory Shenzhen 518055China University of Chinese academy of Sciences Beijing 100049China State Key Laboratory of Microbial Metabolism School of Life Sciences&BiotechnologyShanghai Jiao Tong UniversityShanghai 200240China State Key Laboratory of Functions and Applications of Medicinal Plants Guizhou Medical UniversityGuiyang 550014China Molecular biology Experimental Center Germplasm Bank of Wild Species in Southwest ChinaKunming Institute of BotanyChinese Academy of SciencesKunming 650201China Yunnan Key Laboratory for Wild Plant Resources Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China Research Unit of Chemical biology of Natural Anti-Virus Products Chinese Academy of Medical SciencesBeijing 100730China

In the intricate process of natural product biosynthesis,a metabolon can enhance metabolic flux by associating sequential enzymes.A fungal metabolon,comprising of flavin-dependent monooxygenase Spe F and P450 monooxygenase Spe G,is identified in the biosynthesis of spiro polycyclic alkaloids(+)-notoamide B and its diastereomer(+)-versicolamide *** notoamide E as a substance,Spe F/Spe G metabolon can control the stereoselectivity of its 2,3-epoxidation,followed by hydrogen atom ion at C-17 to generate reactive epoxide tau-m A with dienyl iminium ***,(+)-notoamide B and(+)-versicolamide B are produced via tandem nonenzymatic inverse-electron-demand Diels-Alder reaction and semipinacol *** provides the first example of metabolon in the biosynthesis of spiro-prenylated indole alkaloids.

关键词： metabolon Diels-Alder reaction semipinacol rearrangement biosynthesis notoamides spiro polycyclic alkaloids

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A comprehensive review of phytochemical approaches in treatment of acute myeloid leukemia:Associated pathways and molecular mechanisms

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Chinese Herbal Medicines 2025年第1期17卷 41-55页

作者： Mouvanal Sajana T.S.Gopenath Basalingappa M.Kanthesh Division of Molecular biology School of Life SciencesJSS AHER Department of biotechnology and Bioinformatics School of Life SciencesJSS AHER

Acute myeloid leukemia(AML) is a type of cancer that affects the blood and bone *** review conducts a thorough analysis of AML, addressing its genetic *** examination extends to the current therapeutic approaches employed for AML,shedding light on their efficacy and the notable side effects experienced by patients undergoing these treatments,leading to a low overall survival ***,exploring alternative treatments,such as phytochemicals,is ***,the review explores the complex landscape of phytochemicals,categorizing them based on their diverse properties,which include alkaloids,phenols,terpenoids,organo-sulfur compounds,and other compounds,including quinones,and elucidating their mechanisms of *** emphasis is placed on their involvement in critical signaling pathways,with a particular focus on how these phytochemicals impact AML when evaluated across a spectrum of cell *** in-depth exploration aims to uncover potential targets within the molecular landscape of AML where phytochemicals can exert their therapeutic *** review investigates the potential role of plant-derived phytochemicals as adjunctive therapies for *** exploration encompasses the identification of specific phytochemicals that exhibit promising anti-leukemic properties and evaluates their potential in clinical *** conventional treatments,the review explores the integration of complementary and alternative medicine as a holistic approach to managing *** examination encompasses the synergy between conventional therapies and alternative interventions,exploring how these combined strategies may enhance overall therapeutic outcomes and mitigate side *** a forward-looking perspective,the overarching goal is to contribute to the evolving landscape of AML treatment by considering innovative approaches that harness the therapeutic potential of phytochemicals,both independently and in conjunction with establishe

关键词： cell death complementary and alternative medicine leukemia phytochemicals signaling pathways therapeutic targets

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Enhancing Plant Resilience to Abiotic Stress:The Power of Biostimulants

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Phyton-International Journal of Experimental Botany 2025年第1期94卷 1-31页

作者： Su-Ee Lau Lucas Wei Tze Lim Mohd Fadhli Hamdan Colin Chan Noor Baity Saidi Janna Ong-Abdullah Boon Chin Tan Centre for Research in biotechnology for Agriculture Universiti MalayaKuala Lumpur50603Malaysia Faculty of Science Universiti MalayaKuala Lumpur50603Malaysia School of Biological Sciences Universiti Sains MalaysiaGeorge Town11800Malaysia School of biology Faculty of Applied SciencesUiTM Shah AlamShah Alam40450Malaysia Department of biotechnology Faculty of Applied SciencesUCSI UniversityCheras56000Malaysia Department of Cell and Molecular biology Faculty of Biotechnology and Biomolecular SciencesUniversiti Putra MalaysiaSerdang43400Malaysia

Abiotic stresses such as drought,heat,salinity,and heavy metal contamination severely affect global agricultural *** 2005 and 2015,droughts caused losses of approximately USD 29 billion in developing countries,and from 2008 to 2018,droughts accounted for over 34%of crop and livestock yield losses,totaling about USD 37 *** support the growing human population,agricultural output must increase substantially,necessitating a 60%–100%rise in crop productivity to meet the escalating *** address environmental challenges,organic,inorganic,and microbial biostimulants are increasingly employed to enhance plant resilience through various morphological,physiological,and biochemical *** biostimulants enhance plant resilience under abiotic stress through mechanisms such as abscisic acid signaling modulation,which regulates stomatal closure to reduce water loss during drought and heat ***,they aid in scavenging reactive oxygen species and stabilizing ion channels,mitigating oxidative damage,and maintaining ionic balance under stress conditions such as *** review summarizes recent advancements in applying these biostimulants,focusing on their roles in triggering morphological,physiological,biochemical,and molecular changes that collectively enhance plant resilience under stress *** also includes a bibliometric analysis of all articles published on biostimulants from 2019 to 2024 and explores future research *** was placed on optimizing biostimulant formulations and understanding their synergistic effects to maximize their efficacy under various stress *** integrating biostimulants into agricultural practices,we can adopt a sustainable strategy to safeguard crop productivity in the face of climate change and environmental stressors.

关键词： Abiotic stress crop improvement microbial plant growth promoting rhizobacteria protein hydrolysate seaweed

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