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Osteocyte morphology and orientation in relation to strain in the jaw bone

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International Journal of Oral Science 2018年第1期10卷 36-43页

作者： Vivian Wu René F. M. van Oers Engelbert A. J. M. Schulten Marco No Helder Rommel G. Bacabac Jenneke Klein-Nulend Department of Oral Cell Biology Academic Centre for Dentistry Amsterdam (ACTA) Amsterdam Movement Sciences University of Amsterdam and Vrije Universiteit Amsterdam Amsterdam The Netherlands Department of Dental Materials Science Academic Centre for Dentistry Amsterdam (ACTA) Amsterdam Movement Sciences University of Amsterdam and Vrije Universiteit Amsterdam Amsterdam The Netherlands Department of Oral and Maxillofacial Surgery VU University Medical Center/Academic Centre for Dentistry Amsterdam (ACTA) Amsterdam Movement Sciences Amsterdam The Netherlands Department of Physics Medical Biophysics Group University of San Carlos Cebu City Philippines

Bone mass is important for dental implant success and is regulated by mechanoresponsive osteocytes. We aimed to investigate the relationship between the levels and orientation of tensile strain and morphology and orientation of osteocytes at different dental implant positions in the maxillary bone. Bone biopsies were retrieved from eight patients who underwent maxillary sinus-floor elevation with β-tricalcium phosphate prior to implant placement. Gap versus free-ending locations were compared using 1) a three-dimensional finite-element model of the maxilla to predict the tensile strain magnitude and direction and 2) histology and histomorphometric analyses. The finite-element model predicted larger, differently directed tensile strains in the gap versus freeending locations. The mean percentage of mineralised residual native-tissue volume, osteocyte number(mean ± standard deviations:97 ± 40/region-of-interest), and osteocyte shape(90% elongated,10% round) were similar for both locations. However, the osteocyte surface area was 1.5-times larger in the gap than in the free-ending locations, and the elongated osteocytes in these locations were more cranially caudally oriented. In conclusion, significant differences in the osteocyte surface area and orientation seem to exist locally in the maxillary bone, which may be related to the tensile strain magnitude and orientation. This might reflect local differences in the osteocyte mechanosensitivity and bone quality, suggesting differences in dental implant success based on the location in the maxilla.

关键词： SINUS FLOOR ELEVATION MINERAL DENSITY MAXILLARY HISTOMORPHOMETRY TOMOGRAPHY IMPLANTS SPINE

GPCR/endocytosis/ERK signaling/S2R is involved in the regulation of the internalization,mitochondria-targeting and-activating properties of human salivary histatin 1

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International Journal of Oral Science 2022年第3期14卷 334-348页

作者： Dandan Ma Wei Sun Cuicui Fu Kamran Nazmi Enno C.I.Veerman Richard T.Jaspers Jan G.M.Bolscher Floris J.Bikker Gang Wu Department of Oral Biochemistry Academic Centre for Dentistry Amsterdam(ACTA)University of Amsterdam and Vrije Universiteit AmsterdamAmsterdamNetherlands School of Stomatology Zhejiang Chinese Medical UniversityHangzhouChina Laboratory for Myology Department of Human Movement SciencesFaculty of Behavioral and Movement SciencesAmsterdam Movement SciencesVrije Universiteit AmsterdamAmsterdamNetherlands Department of Oral Cell Biology Academic Centre for Dentistry Amsterdam(ACTA)University of Amsterdam and Vrije Universiteit AmsterdamAmsterdamNetherlands Department of Oral and Maxillofacial Surgery/Pathology Amsterdam UMC and Academic Center for Dentistry Amsterdam(ACTA)Vrije Universiteit AmsterdamAmsterdam Movement ScienceAmsterdamNetherlands

Human salivary histatin 1(Hst1)exhibits a series of cell-activating properties,such as promoting cell spreading,migration,and metabolic *** recently have shown that fluorescently labeled Hst1(F-Hst1)targets and activates mitochondria,presenting an important molecular ***,its regulating signaling pathways remain to be *** investigated the influence of specific inhibitors of G protein-coupled receptors(GPCR),endocytosis pathways,extracellular signal-regulated kinases1/2(ERK1/2)signaling,p38 signaling,mitochondrial respiration and Na+/K+-ATPase activity on the uptake,mitochondria-targeting and-activating properties of *** performed a si RNA knockdown(KD)to assess the effect of Sigma-2 receptor(S2R)/Transmembrane Protein 97(TMEM97)—a recently identified target protein of *** also adopted live cell imaging to monitor the whole intracellular trafficking process of *** results showed that the inhibition of cellular respiration hindered the internalization of *** inhibitors of GPCR,ERK1/2,phagocytosis,and clathrin-mediated endocytosis(CME)as well as siRNA KD of S2R/TMEM97 significantly reduced the uptake,which was accompanied by the nullification of the promoting effect of F-Hst1 on cell metabolic *** the inhibitor of CME and KD of S2R/TMEM97 significantly compromised the mitochondria-targeting of *** further showed the intracellular trafficking and targeting process of F-Hst1,in which early endosome plays an important ***,phagocytosis,CME,GPCR,ERK signaling,and S2R/TMEM97 are involved in the internalization of Hst1,while only CME and S2R/TMEM97 are critical for its subcellular *** inhibition of either internalization or mitochondria-targeting of Hst1 could significantly compromise its mitochondria-activating property.

关键词： GPCR/endocytosis/ERK signaling/S2R is involved in the regulation of the internalization mitochondria-targeting and activating properties of human salivary histatin 1 ERK

Oral polymorphonuclear neutrophil characteristics in relation to oral health： a cross-sectional, observational clinical study

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International Journal of Oral Science 2016年第3期8卷 191-198页

作者： Patrick Rijkschroeff Ineke DC Jansen Fridus A van der Weijden Bart IF Keijser Bruno G Loos Elena A Nicu Department of Periodontology Academic Centre for Dentistry Amsterdam Amsterdam The Netherlands Department of Preventive dentistry Academic Centre for Dentistry Amsterdam Amsterdam The Netherlands Microbiology and Systems Biology TNO Earth Environmental and Life Sciences Zeist The Netherlands Top Institute Food and Nutrition Wageningen The Netherlands

Polymorphonuclear neutmphils （PMNs） have a major role in the innate immune system. However, little is known about PMN contribution in relation to oral health. The objective of this study was to investigate the numbers and functional characteristics of oral PMNs （oPMNs） compared with circulatory PMNs （cPMNs）. Oral rinse and venous blood samples were obtained from 268 systemically and orally healthy volunteers in a cross-sectional observational study. PMN counts, cell cycle analysis and cellular activation state were investigated. Also, reactive oxygen species （ROS） production was analyzed, with and without bacterial stimulation （Fusobacterium nucleatum）. In males, 1.2 × 10^6- 1.0 ×10^6 oPMNs were collected, and showed a tendency to correlate with the levels of gingival bleeding （r= 0.215, P= 0.008）. Comparable oPMNs counts were found among females （1.0 ×10^6＋ 0.7 × 10^6）. More late-stage apoptotic/necrotic cells were found among the oPMNs （53.1%） compared with the cPMNs （8.5%; P〈 0.001）. Without additional stimulation, oPMNs were more activated than cPMNs, as indicated by higher expression of CD11b, CD63 and CD66b, and higher constitutive ROS levels （P〈 0.001）. Notably, in response to bacterial stimulation, oPMNs released comparable ROS levels as cPMNs （P= 0.042）. In conclusion, this study provides data on viable oPMNs showing high levels of activation in orally and systemically healthy individuals, free of apparent caries lesions and periodontal disease. These data suggests that although the oPMNs are in a more mature stage of their life cycle compared with the cPMNs, oPMNs are still responsive to stimulation, which indicates their functional potential and possible contribution to a healthy oral ecosystem.

关键词： apoptosis degranulation neutrophils oral health reactive oxygen species

Co-administration of platelet-rich plasma and small intestinal submucosa is more beneficial than their individual use in promoting acute skin wound healing

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Burns & Trauma 2021年第1期9卷 72-82页

作者： Xiaoxuan Lei Liuhanghang Cheng Yu Yang Mengru Pang Yunqing Dong Xuanru Zhu Caihong Chen Zexin Yao Gang Wu Biao Cheng Tymour Forouzanfar Department of Oral and Maxillofacial Surgery/Pathology Amsterdam UMC and Academic Center for Dentistry Amsterdam(ACTA)Vrije Universiteit AmsterdamAmsterdam Movement ScienceAmsterdam1081HVThe Netherlands Department of Burn and Plastic Surgery General Hospital of Southern Theater CommandGuangzhou510030China Department of Oral Implantology and Prosthetic dentistry Academic Center for Dentistry Amsterdam(ACTA)University of Amsterdam(UvA)and Vrije Universiteit Amsterdam(VU)Amsterdam1081LAThe Netherlands

Background:Acute skin wounds may compromise the skin barrier,posing a risk of *** intestinal submucosa(SIS)is widely used to treat acute and chronic ***,the efficacy of SIS to accelerate wound healing still needs to be improved to meet clinical *** tackle this problem,platelet-rich plasma(PRP)is used due to its potency to promote proliferation,migration and adhesion of target *** this study,we applied PRP and SIS to skin wounds to explore their effects on wound healing by evaluating re-epithelialization,collagen production,angiogenesis and the inflammatory ***:A1×1-cm full-thickness skin defectwas established in *** mice were divided into four treatment groups:PRP+SIS,PRP,SIS and *** days 3,5,7,10 and 14 post-surgery,tissue specimens were *** and eosin,Masson’s trichrome,immunohistochemical and immunofluorescence double staining were used to visualize epidermal thickness,collagen and vascular regeneration and ***:Wound contraction in the PRP and PRP+SIS groups was significantly greater,compared with the other groups,on days 3 and 5 post-surgery.A histological analysis showed higher collagen expression in the PRP and PRP+SIS groups on day 7,whichwas associated with a thicker epidermal layer on day *** addition,immunohistochemical staining showed that CD31-positive blood vessels and vascular endothelial growth factor expression in the PRP+SIS and PRP groups were significantly higher,compared with the control ***,immunofluorescence double staining showed that the number of M1 and M2 macrophages in the PRP+SIS and PRP groups was higher,compared with the control and SIS groups alone,on day ***,on day 7,the number of M1 macrophages dramatically decreased in the PRP+SIS and PRP *** ratio of M2 to M1 macrophages in the PRP+SIS and PRP groups was 3.97 and 2.93 times that of the control group and 4.56 and 3.37 times that of the SIS group,respectively.

关键词： Small intestinal submucosa Platelet-rich plasma Full-thickness skin defect M1macrophages M2macrophages Animal model wound healing skin

Chondroinductive/chondroconductive peptides and their-functionalized biomaterials for cartilage tissue engineering

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Bioactive Materials 2022年第3期7卷 221-238页

作者： Mingjing Zhu Wenchao Zhong Wei Cao Qingbin Zhang Gang Wu Department of Temporomandibular Joint Affiliated Stomatology Hospital of Guangzhou Medical UniversityGuangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative MedicineGuangzhouGuangdong510182China Department of Oral and Maxillofacial Surgery/Pathology Amsterdam UMC and Academic Center for Dentistry Amsterdam(ACTA)Vrije Universiteit Amsterdam(VU)Amsterdam Movement Science(AMS)Amsterdamthe Netherlands Department of Oral Cell Biology Academic Centre for Dentistry Amsterdam(ACTA)University of Amsterdam and Vrije Universiteit AmsterdamAmsterdam Movement SciencesAmsterdamthe Netherlands

The repair of articular cartilage defects is still challenging in the fields of orthopedics and maxillofacial surgery due to the avascular structure of articular cartilage and the limited regenerative capacity of mature *** provide viable treatment options,tremendous efforts have been made to develop various chondrogenically-functionalized biomaterials for cartilage tissue *** that are derived from and mimic the functions of chondroconductive cartilage extracellular matrix and chondroinductive growth factors,represent a unique group of bioactive agents for chondrogenic *** they can be chemically synthesized,peptides bear better reproducibility,more stable efficacy,higher modifiability and yielding efficiency in comparison with naturally derived biomaterials and recombinant growth *** this review,we summarize the current knowledge in the designs of the chondroinductive/chondroconductive peptides,the underlying molecular mechanisms and their-functionalized biomaterials for cartilage tissue *** also systematically compare their in-vitro and in-vivo efficacies in inducing *** vision is to stimulate the development of novel peptides and their-functionalized biomaterials for cartilage tissue engineering.

关键词： Chondroinductive Chondroconductive Peptide Biomaterial Cartilage tissue engineering

Chemical composition of Galla chinensis extract and the effect of its main component(s) on the prevention of enamel demineralization in vitro

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International Journal of Oral Science 2012年第3期4卷 146-151页

作者： Xue-Lian Huang Ming-Dong Liu Ji-Yao Li Xue-Dong Zhou Jacob M ten Cate State Key Laboratory of Oral Diseases Sichuan UniversityChengduChina West China Hospital of Stomatology Sichuan UniversityChengduChina Department of Cariology Endodontology Pedodontology Academic Centre for Dentistry Amsterdam(ACTA)University of Amsterdam and Free University AmsterdamAmsterdamThe Netherlands Department of Analytical Toxicology West China School of Preclinical and Forensic MedicineSichuan UniversityChengduChina

To determine the chemical composition of Galla chinensisextract （GCE） by several analysis techniques and to compare the efficacy of GCE and its main component（s） in inhibition of enamel demineralization, for the development of future anticaries agents, main organic composition of GCE was qualitatively determined by liquid chromatography-time of flight-mass spectrometry （LC-TOF-MS） and quantified by high-performance liquid chromatography-diode array detector （HPLC-DAD）. Inorganic ions were tested by inductively coupled plasma-atomic emission spectroscopy and F was especially measured by ion chromatography. Then, bovine enamel blocks were randomly divided into four treatment groups and were subjected to a pH-cycling regime for 12 times. Each cycle included 5-min applications with one of four treatments： 4g.L-1 GCE solution, 4g.L-1 gallic acid （GA） solution, I g.L-1 NaF solution （positive control）, deionized water （DDW, negative control）, and then 60-min application in pH 5.0 acidic buffer and 5-min application in neutral buffer. Acidic buffers were retained for calcium analysis. The main organic composition of GCE were GA and its isomer, and, to a lesser extent, small molecule gallotannins. The content of GA in GCE was 71.3%±0.2% （w/w）. Inorganic ions were present in various amounts, of which Ca was （136±2.82） μg.g-1, and Zn was （6.8±0.1） μg.g-1. No F was detected in GCE. in pH cycling, GA showed an effect similar to GCE in inhibiting enamel demineralization （P〉0.05）. GA was found to be the main effective, demineralization inhibiting component of GCE and could be a promising agent for the development of anticaries agents.

关键词： active component Galla chinensis gallic acid composition dental caries

Interleukin-1βinduces human cementoblasts to support osteoclastogenesis

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International Journal of Oral Science 2017年第4期9卷 216-223页

作者： nam c-n huynh vincent everts prasit pavasant ruchanee s ampornaramveth research unit on oral microbiology and immunology microbiology departmentfaculty of dentistrychulalongkorn universityBangkokThailand mineralized tissue research unit faculty of dentistrychulalongkorn universityBangkokThailand department of dental basic sciences faculty of odonto-stomatologyuniversity of medicine and pharmacy at ho chi minh cityHO Chi Minh CityVietnam department of oral cell biology academic centre for dentistry amsterdam(acta)university of amsterdam and vu university amsterdamThe Netherlands department of anatomy faculty of dentistrychulalongkorn universityBangkokThailand

Injury of the periodontium followed by inflammatory response often leads to mot resorption. Resorption is accomplished by osteoclasts and their generation may depend on an interaction with the cells in direct contact with the root, the cementoblasts. Our study aimed to investigate the role of human cementoblasts in the formation of osteoclasts and the effect of interleukin （IL）- 1β hereupon. Extracted teeth from healthy volunteers were subjected to sequential digestion by type I collagenase and trypsin. The effect of enzymatic digestion on the presence of cells on the root surface was analyzed by histology. Gene expression of primary human cementoblasts （pHCB） was compared with a human cementoblast cell line （HCEM）. The pHCBs were analyzed for their expression of IL-1 receptors as well as of receptor activator of nuclear factor kappa-B ligand （RANKL） and osteoprotegerin （OPG）. In a co-culture system consisting of osteoclast precursors （blood monocytes） and pHCBs, the formation of osteoclasts and their resorptive activity was assessed by osteo-assay and ivory slices. The cells obtained after a 120 min enzyme digestion expressed the highest level of bone sialoprotein, similar to that of HCEM. This fraction of isolated cells also shared a similar expression pattern of IL-1 receptors （ILl-R1 and ILl-R2）. Treatment with IL-11~ potently upregulated RANKL expression but not of OPG. pHCBs were shown to induce the formation of functional osteoclasts. This capacity was significantly stimulated by pretreating the pHCBs with IL-1β prior to their co-culture with human blood monocytes. Our study demonstrated that cementoblasts have the capacity to induce osteoclastogenesis, a capacity strongly promoted by IL-1β. These results may explain why osteoclasts can be formed next to the root of teeth.

关键词： cementoblast interleukin-ll3 osteoclast receptor activator of nuclear factor kappa-B ligand tooth resorption

Neuro-regenerative imidazole-functionalized GelMA hydrogel loaded with hAMSC and SDF-1α promote stem cell differentiation and repair focal brain injury

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Bioactive Materials 2021年第3期6卷 627-637页

作者： Yantao Zheng Gang Wu Limei Chen Ying Zhang Yuwei Luo Yong Zheng Fengjun Hu Tymor Forouzanfar Haiyan Lin Bin Liu Emergency Department Zhujiang HospitalSouthern Medical UniversityGuangzhouChina Department of Oral Implantology and Prosthetic dentistry Academic Centre for Dentistry Amsterdam(ACTA)University of Amsterdam(UvA)and Vrije Universiteit Amsterdam(VU)AmsterdamNetherlands Department of Emergency Guangzhou Red Cross HospitalMedical CollegeJinan UniversityGuangzhou City510220China Rheumatology Department The Third Affiliated Hospital of Southern Medical UniversityGuangzhouChina Emergency Medicine Department Qingxin Section of Qingyuan People's HospitalQingyuanChina Institute of Information Technology Zhejiang Shuren UniversityHangzhouChina Department of Oral and Maxillofacial Surgery/Pathology Amsterdam UMC and Academic Center for Dentistry Amsterdam(ACTA)Vrije Universiteit AmsterdamAmsterdam Movement Sciencede Boelelaan1117AmsterdamNetherlands Savaid Stomatology School Hangzhou Medical CollegeHangzhouChina

Brain tissues that are severely damaged by traumatic brain injury(TBI)is hardly regenerated,which leads to a cavity or a repair with glial ***-cell therapy is one viable option to treat TBI-caused brain tissue damage,whose use is,whereas,limited by the low survival rate and differentiation efficiency of stem *** approach this problem,we developed an injectable hydrogel using imidazole groups-modified gelatin methacrylate(GelMA-imid).In addition,polydopamine(PDA)nanoparticles were used as carrier for stromal-cell derived factor-1(SDF-1α).GelMA-imid hydrogel loaded with PDA@SDF-1αnanoparticles and human amniotic mesenchymal stromal cells(hAMSCs)were injected into the damaged area in an in-vivo cryogenic injury model in *** hydrogel had low module and its average pore size was 204.61±41.41 nm,which were suitable for the migration,proliferation and differentiation of stem ***-vitro cell scratch and differentiation assays showed that the imidazole groups and SDF-1αcould promote the migration of hAMSCs to injury site and their differentiation into nerve *** highest amount of nissl body was detected in the group of GelMA-imid/SDF-1α/hAMSCs hydrogel in the in-vivo ***,histological analysis showed that GelMA-imid/SDF-1α/hAMSCs hydrogel could facilitate the regeneration of regenerate endogenous nerve *** summary,the GelMA-imid/SDF-1α/hAMSCs hydrogel promoted homing and differentiation of hAMSCs into nerve cells,and showed great application potential for the physiological recovery of TBI.

关键词： Injectable hydrogel Imidazole Stromal-cell derived factor-1(SDF-1α) Human amniotic mesenchymal stromal cells (hAMSCs) Brain injury

Regulation of hypoxic stress and oxidative stress in bone grafting: Current trends and future perspectives

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Journal of Materials Science & Technology 2023年第26期157卷 144-153页

作者： Hao Hu Xiao Liu Jun Chen Shangbin Cui Hualin Yi Gang Wang Renxian Wang Tiansheng Zheng Ben Wan Zhiyu Zhou Yong Wan Manman Gao Dafu Chen Xuenong Zou Guangdong Provincial Key Laboratory of Orthopaedics and Traumatology Department of Spinal SurgeryThe First Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510080China Laboratory of Bone Tissue Engineering Beijing Laboratory of Biomedical MaterialsBeijing Research Institute of Traumatology and OrthopaedicsBeijing Jishuitan HospitalBeijing 100035China Department of Orthopedics The First Affiliated Hospital of Gannan Medical UniversityGanzhouChina Department of Oral and Maxillofacial Surgery/Pathology Amsterdam UMC and Academic Center for Dentistry Amsterdam(ACTA)Vrije Universiteit Amsterdam(VU)Amsterdam Movement Science(AMS)Amsterdamthe Netherlands

Tissue engineering aims to offer large-scale replacement of damaged organs using implants with the com-bination of cells,growth factors and ***,the intra/peri-implant region is exposed to se-vere hypoxic stress and oxidative stress during the early stage of implantation with bone graft materials,which endangers the survival,proliferation and differentiation of seed cells within the implants as well as the host cells surrounding the *** the bone graft material could spontaneously and intelligently regulate the hypoxic stress and oxidative stress to a moderate level,it will facilitate the vascularization of the implants and the rapid regeneration of the bone *** this review,we will first introduce the signaling pathways of cellular response under hypoxic stress and oxidative stress,then present the clas-sical material designs and examples in response to hypoxic stress and oxidative *** finally,we will address the important role of epigenetic mechanisms in the regulation of hypoxic stress and oxida-tive stress and describe the potential applications and prospective smart bone graft materials based on novel epigenetic factors against hypoxic stress and oxidative stress in bone *** main content of this review is summarized in the following graphical abstract.

关键词： Hypoxic stress Oxidative stress Bone repair Bone graft material Epigenetic regulation