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Recovery of DDBla （DAMAGED DNA BINDING PROTEIN la） in a Screen to Identify Novel RUB-Modified Proteins in Arabidopsis thaliana

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Molecular Plant 2012年第5期5卷 1163-1166页

作者： Sara K. Hotton Meliza F. Castro Richard A. Eigenheer Judy Callis Molecular and Cellular Biology University of California Davis CA 95616 USA Proteomics core facility University of California Davis CA 95616 USA

Dear Editor, RUB/Nedd8 （RELATED TO UBIQUITIN/neuronal precur- sor cell-expressed developmentally downregulated 8） is a 76-amino acid protein that modifies other proteins and has homology to UBIQUITIN （UBQ）. Like UBQ, RUB modifies pro- teins through an isopeptide linkage between its C-terminal carboxyl group and the e-amino group of a lysine in the accep- tor protein and attachment occurs via an E1-E2-E3 enzymatic cascade.

关键词： DNA结合蛋白 Arabidopsis 蛋白质拟南芥屏幕 LA 损坏修饰

Genetic and Proteomic Analyses of a Xanthomonas campestris *** purC Mutant Deficient in Purine Biosynthesis and Virulence

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Journal of Genetics and Genomics 2013年第9期40卷 473-487页

作者： Zhihui Yuan Li Wang Shutao Sun Yao Wu Wei Qian State Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of Sciences University of Chinese Academy of Sciences Center of core facility Institute of MicrobiologyChinese Academy of Sciences

Bacterial proliferation in hosts requires activation of a number of housekeeping pathways, including purine de novo biosynthesis. Although inactivation of purine biosynthesis genes can attenuate virulence, it is unclear which biochemical or virulence factors are associated with the purine biosynthesis pathway in vivo. We report that inactivation of purC, a gene encoding phosphoribosylaminoimidazole-succinocarboxamide synthase, caused complete loss of virulence in Xanthomonas campestris pv. cam- pestris, the causal agent of black rot disease of cruciferous plants. The purC mutant was a purine auxotroph; it could not grow on minimal medium, whereas addition of purine derivatives, such as hypoxanthine or adenine plus guanine, restored growth of the mutant. The purC mutation also significantly enhanced the production of an unknown purine synthesis associated pigment and extracellular polysaccharides by the bacterium. In addition, comparative proteomic analyses of bacteria grown on rich and minimal media revealed that the purC mutation affected the expression levels of diverse proteins involved in purine and pyrimidine synthesis, carbon and energy metabolisms, iron uptake, proteolysis, protein secretion, and signal transduction. These results provided clues to understanding the contributions of purine synthesis to bacterial virulence and interactions with host immune systems.

关键词： Purine biosynthesis Virulence Xanthomonas campestris pv. campestris Proteomics

Old strategies against an old plague: new attenuated tuberculosis vaccines

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医学争鸣 2012年第2期3卷 30-31页

作者： YIN Wen core facility School of Preclinical MedicineFourth Military Medical UniversityXi'an 710032China

Tuberculosis(TB)is still a global health emergency according to WHO and one of the important global control efforts targeting TB is BCG(bacille Calmette-Guérin)***,BCG has been found

关键词：摘要编辑部编辑工作读者

Pulsed laser interference patterning of transition-metal carbides for stable alkaline water electrolysis kinetics

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Carbon Energy 2024年第5期6卷 65-80页

作者： Yewon Oh Jayaraman Theerthagiri Ahreum Min Cheol Joo Moon Yiseul Yu Myong Yong Choi Department of Chemistry(BK21 FOUR) Research Institute of Natural SciencesGyeongsang National UniversityJinjuRepublic of Korea core−facility Center for Photochemistry and Nanomaterials Gyeongsang National UniversityJinjuRepublic of Korea

We investigated the role of metal atomization and solvent decomposition into reductive species and carbon clusters in the phase formation of transition-metal carbides(TMCs;namely,Co_(3)C,Fe_(3)C,TiC,and MoC)by pulsed laser ablation of Co,Fe,Ti,and Mo metals in *** interaction between carbon s-p-orbitals and metal d-orbitals causes a redistribution of valence structure through charge transfer,leading to the formation of surface defects as observed by X-ray photoelectron *** defects influence the evolved TMCs,making them effective for hydrogen and oxygen evolution reactions(HER and OER)in an alkaline ***_(3)C with more oxygen affinity promoted CoO(OH)intermediates,and the electrochemical surface oxidation to Co_(3)O_(4)was captured via in situ/operando electrochemical Raman probes,increasing the number of active sites for OER *** with more d-vacancies exhibits strong hydrogen binding,promoting HER kinetics,whereas Fe_(3)C and TiC with more defect states to trap charge carriers may hinder both OER and HER *** results show that the assembled membrane-less electrolyzer with Co_(3)C∥Co_(3)C and MoC∥MoC electrodes requires~2.01 and 1.99 V,respectively,to deliver a 10 mA cm−2 with excellent electrochemical and structural *** addition,the ascertained pulsed laser synthesis mechanism and unit-cell packing relations will open up sustainable pathways for obtaining highly stable electrocatalysts for electrolyzers.

关键词： acetone H_(2)and O_(2)evolution reactions pulsed laser ablation surface defects transition-metal carbides water electrolyzer

Co-delivery of photosensitizer and diclofenac through sequentially responsive bilirubin nanocarriers for combating hypoxic tumors

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Acta Pharmaceutica Sinica B 2022年第3期12卷 1416-1431页

作者： Yang Zhou Fan Tong Weilong Gu Siqin He Xiaotong Yang Jiamei Li Yue-Dong Gao Huile Gao Key Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial TechnologyWest China School of PharmacySichuan UniversityChengdu 610064China core Technology facility of Kunming Institute of Zoology Chinese Academy of SciencesKunming 650223China Chinese Academy of Sciences Territorial core facility of Kunming Biological Diversity Regional Center Kunming 650223China

Considering that photodynamic therapy(PDT)-induced oxygen consumption and microvascular damage could exacerbate hypoxia to drive more glycolysis and angiogenesis, a novel approach to potentiate PDT and overcome the resistances of hypoxia is avidly needed. Herein, morpholine-modified PEGylated bilirubin was proposed to co-deliver chlorin e6, a photosensitizer, and diclofenac(Dc). In acidic milieu, the presence of morpholine could enable the nanocarriers to selectively accumulate in tumor cells, while PDT-generated reactive oxidative species(ROS) resulted in the collapse of bilirubin nanoparticles and rapid release of Dc. Combining with Dc showed a higher rate of apoptosis over PDT alone and simultaneously triggered a domino effect, including blocking the activity and expression of lactate dehydrogenase A(LDHA), interfering with lactate secretion, suppressing the activation of various angiogenic factors and thus obviating hypoxia-induced resistance-glycolysis and angiogenesis. In addition, inhibition of hypoxia-inducible factor-1a(HIF-1a) by Dc alleviated hypoxia-induced resistance. This study offered a sequentially responsive platform to achieve sufficient tumor enrichment, on-demand drug release and superior anti-tumor outcomes in vitro and in vivo.

关键词： Bilirubin nanoparticles ROS-responsive drug release Charge reversal Photodynamic therapy Hypoxia Diclofenac LDHA inhibition HIF-1a inhibition

BAG6 restricts pancreatic cancer progression by suppressing the release of IL33-presenting extracellular vesicles and the activation of mast cells

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Cellular & Molecular Immunology 2024年第8期21卷 918-931页

作者： Bilal Alashkar Alhamwe Viviane Ponath Fahd Alhamdan Bastian Dörsam Clara Landwehr Manuel Linder Kim Pauck Sarah Miethe Holger Garn Florian Finkernagel Anna Brichkina Matthias Lauth Dinesh Kumar Tiwari Malte Buchholz Daniel Bachurski Sabrina Elmshäuser Andrea Nist Thorsten Stiewe Lisa Pogge von Strandmann Witold Szymański Vanessa Beutgen Johannes Graumann Julia Teply-Szymanski Corinna Keber Carsten Denkert Ralf Jacob Christian Preußer Elke Pogge von Strandmann Institute for Tumor Immunology Philipps-University35043MarburgGermany core facility Extracellular Vesicles Philipps-University35043MarburgGermany Department of Anesthesiology Critical Careand Pain MedicineCardiac Anesthesia DivisionBoston Children’s HospitalBostonUSA Department of Immunology and Anaesthesia Harvard Medical SchoolBostonMAUSA Translational Inflammation Research Division&core facility for Single Cell Multiomics Philipps-University35043MarburgGermany core facility Bioinformatics Philipps-University35043MarburgGermany Clinic for Gastroenterology Endocrinology and MetabolismCenter for Tumor and Immune BiologyPhilipps-University35043MarburgGermany Institute of Systems Immunology Philipps-University35043MarburgGermany Cluster of Excellence on Cellular Stress Responses in Aging-Associated Diseases(CECAD) University of CologneCologneGermany Institute of Molecular Oncology and Genomics core facility Member of the German Center for Lung Research(DZL)Philipps-University35043MarburgGermany Institute of Lung Health Justus Liebig University35392GiessenGermany Institute of Translational Proteomics&core facility Translational Proteomics Biochemical/Pharmacological CentrePhilipps-University35043MarburgGermany Institute of Pathology Philipps-University Marburg and University Hospital Marburg(UKGM)MarburgGermany Department of Cell Biology and Cell Pathology Philipps-University35043MarburgGermany

Recent studies reveal a critical role of tumor cell-released extracellular vesicles(EVs)in pancreatic cancer(PC)***,driver genes that direct EV function,the EV-recipient cells,and their cellular response to EV uptake remain to be ***,we studied the role of Bcl-2-associated-anthanogene 6(BAG6),a regulator of EV biogenesis for cancer *** used a Cre recombinase/LoxP-based reporter system in combination with single-cell RNA sequencing to monitor in vivo EV uptake and tumor microenvironment(TME)changes in mouse models for pancreatic ductal adenocarcinoma(PDAC)in a Bag6 pro-or deficient *** vivo data were validated using mouse and human organoids and patient *** data demonstrated that Bag6-deficient subcutaneous and orthotopic PDAC tumors accelerated tumor growth dependent on EV ***,this was attributed to mast cell(MC)activation via EV-associated *** MCs promoted tumor cell proliferation and altered the composition of the TME affecting fibroblast polarization and immune cell *** cell proliferation and fibroblast polarization were mediated via the MC secretome containing high levels of PDGF and *** with high BAG6 gene expression and high protein plasma level have a longer overall survival indicating clinical *** current study revealed a so far unknown tumor-suppressing activity of BAG6 in ***6-deficiency allowed the release of EV-associated IL33 which modulate the TME via MC activation promoting aggressive tumor *** depletion using imatinib diminished tumor growth providing a scientific rationale to consider imatinib for patients stratified with low BAG6 expression and high MC infiltration.

关键词： Pancreatic cancer EVs BAG6 Mast cells

FFAR4 improves the senescence of tubular epithelial cells by AMPK/SirT3 signaling in acute kidney injury

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Signal Transduction and Targeted Therapy 2022年第12期7卷 4435-4449页

作者： Letian Yang Bo Wang Fan Guo Rongshuang Huang Yan Liang Lingzhi Li Sibei Tao Ting Yin Ping Fu Liang Ma Kidney Research Institute National Clinical Research Center for Geriatrics and Division of NephrologyWest China Hospital of Sichuan UniversityChengdu610041China Research core facility of West China Hospital Chengdu610041China

Acute kidney injury(AKI)is a serious clinical complication with high morbidity and mortality *** substantial progress in understanding the mechanism of AKI,no effective therapy is available for treatment or *** previously found that G protein-coupled receptor(GPCR)family member free fatty acid receptor 4(FFAR4)agonist TUG891 alleviated kidney dysfunction and tubular injury in AKI ***,the versatile role of FFAR4 in kidney has not been well *** the study,the expression of FFAR4 was abnormally decreased in tubular epithelial cells(TECs)of cisplatin,cecal ligation/perforation and ischemia/reperfusion injury-induced AKI mice,*** and conditional TEC-specific knockout of FFAR4 aggravated renal function and pathological damage,whereas FFAR4 activation by TUG-891 alleviated the severity of disease in cisplatin-induced AKI ***,FFAR4,as a key determinant,was firstly explored to regulate cellular senescence both in injured kidneys of AKI mice and TECs,which was indicated by senescence-associatedβ-galactosidase(SA-β-gal)activity,marker protein p53,p21,Lamin B1,phospho-histone H2A.X,phospho-Rb expression,and secretory phenotype IL-6 ***,pharmacological activation and overexpression of FFAR4 reversed the decrease of aging-related SirT3 protein,where FFAR4 regulated SirT3 expression to exhibit anti-senescent effect via Gq subunit-mediated CaMKKβ/AMPK signaling in cisplatin-induced mice and *** findings highlight the original role of tubular FFAR4 in cellular senescence via AMPK/SirT3 signaling and identify FFAR4 as a potential drug target against AKI.

关键词： kidney injury Acute

BRF1 ameliorates LPS-induced inflammation through autophagy crosstalking with MAPK/ ERK signaling

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Genes & Diseases 2018年第3期5卷 226-234页

作者： Weiwei Xie Wei Zheng Min Liu Qizhong Qin Yunpeng Zhao Zhi Cheng Fengjin Guo Department of Cell Biology and Genetics Core Facility of Development BiologyChongqing Medical UniversityChongqing400016China Department of Orthopaedic Surgery Qilu HospitalShandong UniversityJinan250012China

Inflammation is indispensable for host defense,whereas excessive inflammation often develop inflammatory *** is thought to be engaged in many extracellular stress responses,such as starvation and innate ***,autophagy plays an important role in maintaining *** purpose of this study was to elucidate the function of BRF1 in the regulation of inflammation and autophagy response in *** found that BRF1 inhibited the LPS-induced inflammatory factors expression and the autophagy flux in ***,inhibition autophagy with 3-MA can attenuate the suppressive effect of BRF1 on LPS-mediated *** addition,MAPK/ERK signaling pathway was involved in the BRF1 inhibition inflammation and autophagy in *** findings indicate that BRF1 attenuates LPS-induced inflammatory factors secretion through autophagy,at least in part,through MAPK/ERK signaling pathway.

关键词： Autophagy BRF1 Inflammation Macrophage MAPK/ERK

Circle of Willis variants are not associated with thrombectomy outcomes

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Stroke & Vascular Neurology 2021年第2期6卷 310-313页

作者： Thomas Seifert-Held Katharina Eberhard Soren Christensen Edith Hofer Christian Enzinger Gregory W Albers Maarten Lansberg Department of Neurology Medical University of GrazGrazAustria core facility Computational Bioanalytics Center for Medical ResearchMedical University of GrazGrazAustria Department of Neurology&Neurological Sciences Stanford UniversityStanfordCaliforniaUSA

Background The circle of Willis(COW)is part of the brain collateral *** absence of COW segments may affect functional outcome in patients with ischaemic stroke undergoing endovascular *** In 182 patients in the Diffusion and Perfusion Imaging Evaluation for Understanding Stroke Evolution 2 Study and the CT Perfusion to Predict Response to Recanalisation in Ischaemic Stroke Project,COW anatomy was evaluated on postinterventional magnetic resonance *** absence of the posterior communicating artery or the first segments of posterior or anterior cerebral arteries ipsilateral to the ischaemic infarction was rated as an incomplete *** regression was applied to evaluate an association with the patients’modified Rankin scale(mRS)at 90 days after stroke Results An incomplete ipsilateral COW was not predictive of the patients’mRS at 90 days after *** associations were shown for the patients’baseline National Institutes of Health Stroke Scale(NIHSS),age and reperfusion *** effect size suggests that a significant association of an incomplete COW with the mRS at 90 days may be obtained in cohorts of more than 3000 *** Compared with the established predictors NIHSS,age and reperfusion status,an incomplete COW is not associated with functional outcome after endovascular therapy.

关键词： Willis anatomy incomplete