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CRISPR/Cas9-based functional analysis of yellow gene in the diamondback moth,Plutella xylostella

Insect Science 2021年第5期28卷 1504-1508,I0002页

作者： Yajun Wang Yuping Huang Xuejiao Xu Zhaoxia Liu Jianyu Li Xue Zhan Guang Yang Minsheng You Shijun You State Key Laboratory of Ecological Pest Control for Fujian-Taiwan Crops Institute of Applied EcologyFujian Agriculture and Forestry UniversityFuzhou 350002China Joint International Research Laboratory of Ecological Pest Control Ministry of EducationFuzhou 350002China Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops Ministry of AgricultureFuzhou 350002China adepartment of physiology&neurobiology University of ConnecticutStorrsCT 06269USA Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests Institute of Plant ProtectionFujian Academy of Agricultural SciencesFuzhou 350013China

The diamondback moth,Plutella xylostella(L.),is an economically important pest of cruciferous crops worldwide.This pest is notorious for rapid evolution of the resistance to diferent classes of insecticides,making it increasingly dificult to control.Genetics-based control approaches,through manipulation of target genes,have been reported as promising supplements or alternatives to traditional methods of pest management.Here we identified a gene of pigmentation(yellow)in P.xylostella,Pxyellow,which encodes 1674 bp complementary DNA sequence with four exons and three introns.Using the clustered regularly interspersed palindromic repeats(CRISPR)CRISPR-associated protein 9 system,we knocked out Pxyellow,targeting two sites in Exon III,to generate 272 chimeric mutants(57%of the CRISPR-treated individuals)with color-changed phenotypes of the Ist to 3rd instar larvae,pupae,and adults,indicating that Pxyellow plays an essential role in the body pigmentation of P xlostella.Fitness analysis revealed no significant difference in the oviposition of adults,the hatchability of eggs,and the weight of pupac between homozygous mutants and wildtypes,suggesting that Pxyellow is not directly involved in regulation of growth,development,or reproduction.This work advances our understanding of the genetic and insect science molecular basis for body pigmentation of P xylostella,and opens a wide avenue for development of the genctcally based pest control techniques using Pxyellow as a screening marker.

关键词： CRISPR/Cas9 diamondback moth genetically based control novel marker yellow gene

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Gender Differences in Pulmonary Inflammation Following Systemic Cadmium Administration in Rats

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Biomedical and Environmental Sciences 2010年第4期23卷 293-299页

作者： JELENA STOSIC IVANA MIRKOV SANDRA BELIJ MIROSLAV NIKOLIC ALEKSANDRA POPOV DRAGAN KATARANOVSKI MILENA KATARANOVSKI Department of Ecology Institute for Biological Research "Sinisa Stankovic" Institute for Multidisciplinary Research University of Belgrade Institute of Zoology Faculty of BiologyUniversity of Belgrade Institute of physiology and Biochemistry Faculty of BiologyUniversity of Belgrade

Objective To examine the presence of gender differences in pulmonary inflammation evoked by acute systemic cadmium administration in rats. Methods Presence of basic indicators of lung inflammation （inflammatory cytokine lung content, leukocyte infiltration and activity of cells recovered from lungs by enzyme digestion） was analyzed and compared in animals of the two sexes. Results Intraperitoneal administration of cadmium （1.0 mg/kg） resulted in higher cadmium content in lungs of female rats. Higher tumor necrosis factor （TNF） content was noted in lung homogenates of male rats, while interleukin-6 （IL-6） content was slightly, but signifaicantly greater in lungs of female rats. Increased leukocyte infiltration was observed in lungs of male rats, mainly due to neutrophils. Increased responsiveness to phorbol myristate acetate （PMA） stimulation was noted in cells recovered from lungs of male rats. Rise in intracellular content of myeloperoxidase （MPO） was noted in lung cells from cadmium-treated rats of both sexes, but higher in cells from male rats. Conclusion Presented data documented a more intense pulmonary inflammatory response to systemic cadmium administration in males, with higher IL-6 levels in lungs of female individuals. These sex differences in proinflamatory activity of cadmium in lungs should be taken into consideration in studying the remote toxicity of this heavy metal.

关键词： Rats Sex Intraperitoneal cadmium Pulmonary inflammation

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