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Biodiversity and seasonal variation of benthic macrofauna in Minicoy Island, Lakshadweep,India

Acta Oceanologica Sinica 2014年第10期33卷 58-73页

作者： Dalia Susan v Satheesh Kumar P. Pillai N. G. K Central marine Fisheries Research institute Kochi Kerala 682018 India

From the Indian coast only limited data are available on the benthic fauna of the seagrass communities. In this study, seasonal variation in the distribution of macrobenthos and influence of environmental parameters was explored at four seagrass beds and two mangrove stations along the Minicoy Island, Lakshadweep, India, from September 1999 to August 2001. A total of 160 macrobenthic species from eight major groups represented the macrofauna of the Minicoy Island. Of the identified taxa, molluscs 70（gastropods 41.46%, bivalves 7.5%）, polychaetes 27（16.88%）, crustaceans 30（18.75%）, echinoderms 11（6.88%） and remaining others. Average seasonal abundance of benthic macrofauna ranged from 219 to 711 ind./m2, species diversity varied from 1.45 to 3.64 bits per individual, species richness index ranged from 4.01 to 26.17, evenness 0.69 to 1.66. In general, the higher abundance and species diversity was noticed in southern seagrass stations and northern seagrass stations, but in the mangrove stations comparatively low species diversity was observed. Three-way analysis of variance indicated that all communities resulted as being significantly different between seagrass and mangrove station, mainly when the seasonal interaction was considered. Multivariate analyses were employed to help define benthic characteristic and the relationship between environmental parameters at the six monitoring stations. Results of cluster analyses and multidimensional scale plot suggest that for mangrove region, different physiographic provinces, lower salinity, dissolved oxygen and sediment biotic structure have a higher influence on the species composition and diversity than other oceanographic conditions.

关键词： abundance seagrass macrobenthos mangrove Minicoy Island Lakshadweep

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Ternary surface complexes by the E(%)-pH curve method

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Science China Chemistry 1997年第4期40卷 353-364页

作者：张正斌刘莲生赵宏宾傅有君程凤云赵卫红 institute of marine Chemistry Ocean University of QingdaoQingdao 266003China

A new method for the study of ternary surface complexes at liquid-solid interface,the E(%)-pH curve method,was *** types of E(%)-pH curves,namely,phenomenon of left-right shifts of S-shaped curve,v-shaped curve,monodirection shift curve and X-shaped curve,were ***,apart fromtype (Ⅰ) ternary surface complex -S-O-M-L and type (Ⅱ) ternary surface complex -S-L-M,a newtype of ternary surface complex -S-O-L-M was discovered.

关键词： sea water E(%)-pH curve method liquid-solid interface ternary surface complex

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Efficient large-area flexible organic photovoltaic modules on transparent thin silver electrodes

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Science China Chemistry 2025年

作者： Cong Xie Zedong Xiong Xinlu Liu Wen Wang Yang Liu Xueshi Jiang Xin Lu Lulu Sun Yinhua Zhou Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Kunming institute of Physics

Large-area flexible organic photovoltaic（OPv） modules are challenging to achieve high power conversion efficiency（PCE）.Electrical shunts or shortages generally occur in large-area OPv modules due to the ultrathin（100–200 nm） and soft active layers. Improving the surface smoothness of bottom transparent electrode will be able to suppress the shunts and shortages. In this work, we report a method to fabricate smooth and flexible transparent electrodes based on thin silver film, and further to fabricate efficient large-area flexible OPv modules. The fabricated thin silver transparent electrodes simultaneously have high conductivity, high optical transmittance, good mechanical flexibility and low surface roughness. Humidity exposure（h.e.）treatment on MoO3effectively facilitates the continuous growth of the thin transparent silver electrode. 6 nm silver film on MoO3（h.e.） on plastic substrate shows a transmittance up to 89%, a sheet resistance of 15 Ω/sq, and a low surface roughness with a rootmean-square value of 0.276 nm. Furthermore, 52-cm2flexible organic solar modules（15 subcells） showed a certified PCE of12.66%. Furthermore, a 1350 cm2flexible component assembled by 25 pieces of 52–55 cm2modules showed certified PCE of12.10% with an open-circuit voltage of 62.85 v.

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Protochordate amphioxus is an emerging model organism for comparative immunology

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Progress in Natural Science:Materials International 2009年第8期19卷 923-929页

作者： Shicui Zhang a, Yujun Liang a, Guangdong Ji a, Zhimeng Zhuang b a Department of marine biology, Ocean University of China, Qingdao 266003, China b Yellow Sea Fishery Research institute, Chinese Academy of Fishery Science, Qingdao 266071, China [a]Department of marine biology Ocean University of China Qingdao 266003 China [b]Yellow Sea Fishery Research Institute Chinese Academy of Fishery Science Qingdao 266071 China

Protochordate amphioxus is an extant invertebrate regarded quite recently as a basal chordate. It has a vertebrate-like body plan including a circulation system with an organization similar to that of vertebrates. However, amphioxus is less complex than vertebrates for having a genome uncomplicated by extensive genomic duplication, and lacking lymphoid organs and free circulating blood cells. Recent studies on immunity have demonstrated the presence in amphioxus of both the constituent elements of key molecules involved in adaptive immunity such as proto-major histocompatibility complex (proto-MHC), v region-containing chitin-binding protein (vCBP) and v and C domain-bearing protein (vCP), and the complement system operating via the alternative and lectin pathways resembling those seen in vertebrates. In addition, the acute phase response profile in amphioxus has been shown to be similar to that observed in vertebrates. These findings together with the relative structural and genomic simplicity make amphioxus an ideal organism for gaining insights into the origin and evolution of the vertebrate immune system, especially adaptive immunity, and the composition and mech- anisms of the vertebrate innate immunity.

关键词： Amphioxus Branchiostoma Immunity Evolution

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The first complete mitogenome of Acharax sp.(Protobranchia,Solemyida,Solemyidae):comparisons with other Solemyidae bivalves and deep-sea adaptive characteristics

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Journal of Oceanology and Limnology 2023年第6期41卷 2374-2390页

作者： Mei YANG Jixing SUI Xinzheng LI Department of marine Organism Taxonomy&Phylogeny Institute of OceanologyChinese Academy of SciencesQingdao 266071China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China University of Chinese Academy of Sciences Beijing 100049China Laboratory for marine biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China

Solemyidae is an ancient group of protobranch bivalves,and most solemyids are symbiotic with chemoautotrophic and gill-hosted bacteria,enabling them to survive in unusual habitats such as deepsea chemosynthetic ***,evolution of the mitogenomes in this family and their phylogenetic relationships remain poorly *** complete mitogenome of Acharax *** determined and compared with other available mitogenomes of *** mitogenome of Acharax *** 18970 bp in length and consists of 13 protein-coding genes,2 ribosomal RNA genes,and 22 transfer RNA *** gene arrangement was identical to those of other sequenced *** the present five mitogenomes of Solemyidae species,all protein-coding genes were initiated with the typical ATD(ATA,ATG,and ATT)codon and terminated with the TAA/TAG ***,the incomplete termination codon was *** Ka/Ks ratio analyses indicated that 13 protein-coding genes of five Solemyidae mitogenomes suffered strong purifying *** to 45 existing shallow water equivalents,the 18 available mitogenomes from the deep-sea,including the Acharax *** this study,show significantly more nonpolar amino acids in the 13 protein-coding genes,which indicates the adaptation to the deep-sea *** phylogenetic tree based on 48 Bivalvia complete mitogenomes provided further information to support the scientific classification of *** relationships among Solemyidae were assessed based on 2 mitochondrial(16S rRNA and COX1)and 3 nuclear(18S rRNA,28S rRNA,and histone H3)gene sequences from 17 in-group *** two genera Acharax and Solemya formed a monophyletic clade each,and Acharax *** with previously reported Acharax bivalves with high support values.

关键词： Solemyidae Acharax mitogenome deep sea adaptation phylogeny

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Two new species of the genus Munidopsis Whiteaves,1874(Crustacea:Anomura:Munidopsidae)from the Caroline Ridge,South of the Mariana Trench

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Journal of Oceanology and Limnology 2021年第5期39卷 1841-1853页

作者： Dong DONG Xinzheng LI Department of marine Organism Taxonomy&Phylogeny Institute of OceanologyChinese Academy of SciencesQingdao 266071China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao266071China University of Chinese Academy of Sciences Beijing 100049China Laboratory for marine biology and Biotechnology Pilot NationalLaboratory for Marine Science and Technology(Qingdao)Qingdao 266237China

The genus Munidopsis has high biodiversity in seamount *** this study,two new Munidopsis species,Munidopsis ahyongi *** Munidopsis carolinensis *** the Caroline Ridge in the tropical western Pacific Ocean were *** *** very similar to a group of species in the *** complex,but can be readily distinguished from the related species in having the oblique posterior orbital margins,2 spines on the lateral carapace margin,and a relatively longer rostral median *** *** different from two related species primarily in having oblique frontal margins,blunt outer orbital angles,straight lateral margins of the rostrum,scale-like rugae on sternites 5-7,and less spinose pereopod 1(chelipeds,P1)merus and *** from the morphological comparisons,DNA barcode analysis and phylogenetic analysis based on the cytochrome c oxidase subunit I gene(COI)were employed for the identification of these new species.

关键词： Munidopsis new species seamount tropical western Pacific Ocean DNA barcodes

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Molecular response and developmental speculations in metamorphosis of the veined rapa whelk,Rapana venosa

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Integrative Zoology 2023年第3期18卷 506-517页

作者： Pu SHI Hao SONG Jie FENG Meijie YANG Zhenglin YU Zhi HU Cong ZHOU Haizhou LI Tao ZHANG CAS Key Laboratory of marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of SciencesQingdaoChina Laboratory for marine Ecology and Environmental Science Qingdao National Laboratory for Marine Science and TechnologyQingdaoChina Center for Ocean Mega-Science Chinese Academy of SciencesQingdaoChina CAS Engineering Laboratory for marine Ranching Institute of OceanologyChinese Academy of SciencesQingdaoChina University of Chinese Academy of Sciences BeijingChina Shandong Province Key Laboratory of Experimental marine biology QingdaoChina FuHan Ocean Technology Co. LtdHaiyangChina

Metamorphosis is the short developmental stage characterized by dramatic ontogenetic changes that occurs in most ***,this important process remains largely unclear in marine *** this study,we performed the sequential RNA sequencing of a representative mollusk,the rapa whelk(Rapana venosa),that is undergoing metamorphosis and conducted differential gene expression analysis and weighted gene co-expression network analysis(WGCNA)to investigate the overall and dynamic transcriptome *** results revealed that the expression of cytochrome P4502A and 3A were upregulated during metamorphosis,while the expression of H/ACA ribonucleoproteins increased 4 h after metamorphosis induction(M4 stage),indicating that *** mainly responded to the pelagobenthic *** the M24 stage,the enrichment of v-type proton ATPase and insulin indicated the complete development of secretory organs and initiation of hormone ***,at the M48 stage,the enrichment of zinc metalloproteinase and conotoxin indicated a well-developed predation system that requires exogenous ***,during the PL stage,the genes associated with growth control were highly enriched,implying that *** had completed metamorphosis and has entered the period of rapid ***,our study provides useful transcriptomic resources for *** and contributes new insights that may assist in elucidating the mechanisms underlying metamorphosis in marine invertebrates.

关键词： metamorphosis mollusk rapa whelk transcriptomics

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Geochemical characteristics of hydrothermal sediments from Iheya North Knoll in the Okinawa Trough

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Chinese Journal of Oceanology and Limnology 2017年第4期35卷 947-955页

作者：胡倩男张鑫蒋富清王冰栾振东陈长安阎军 Key Laboratory of marine Geology and Environment Institute of OceanologyChinese Academy of Sciences Laboratory for marine Geology Qingdao National Laboratory for Marine Science and Technology University of Chinese Academy of Sciences

Thirty sediment subsamples were recovered from the Iheya North hydrothermal field （with an average of 38 m away from the hydrothermal vent） in the middle Okinawa Trough. Samples were obtained by the ROv （Remote Operated vehicle） Faxian during the virgin cruise of the R/v Kexue in 2014 with the application of push cores. The chemical compositions of the sediments show that the hydrothermal sediments near the hydrothermal vent are mainly composed of SO3, ZnO and Fe203. Moreover, the hydrothermal sediments are also highly enriched in Pb, As, Sb, Hg, Se, Ag, Ba, Mo and Cd comparing with previous analysis results. On the other hand, the concentrations of St, Hg andAg in studied sediments are strongly and positively correlated, these elements can be used as an hydrothermal indicator. In addition, a factor analysis of the sediments suggested that the sediments were mainly influenced by hydrothermal origin, and terrestrial and biogenic input are limited in studied area. It is also suggested that different stages of crystallization were involved in the formation of hydrothermal chimney from factor analysis.

关键词： hydrothermal vent hydrothermal sediments Okinawa Trough

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Rapid separation of acetophenone and its monohydroxy isomers by capillary electrophoresis

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Chinese Chemical Letters 2013年第9期24卷 833-836页

作者： Ramaiyan Sekar Suresh Kumar Kailasa Wen-Shan Li Han-Chung Wu Hui-Fen Wu Department of Chemistry National Sun Yat-Sen University Depatment of Applied Chemistry S.V. National Institute of Technology Doctoral Degree Program in marine Biotechnology National Sun Yat-Sen University institute of Chemistry Academia Sinica institute of Cellular and Organismic biology Academia Sinica Center of Nanoscience and Nanotechnology National Sun Yat-Sen University School of Pharmacy College of Pharmacy Kaohsiung Medical University

This work describes the development of a capillary electrophoresis （CE） method for the simultaneous separation of acetophenone （AP）, 2-hydroxyacetophenone （2-HAP）, 3-hydroxyacetophenone （3-HAP） and 4-hydroxyacetophenone （4-HAP） in synthetic mixtures using 10 mmol/L of sodium tetraborate buffer （pH 9.5）. The aim of this work is to demonstrate the effectiveness of CE to separate AP and its monohydroxy isomers and to define how the separations are affected by buffers, buffer pH, sample matrices and separation voltage. This method was successfully used for the trace level separation and determination of 2-HAP, 3-HAP and 4-HAP in synthetic mixture and 4-HAP in spiked plasma samples.

关键词： Capillary electrophoresis Electrokinetic injection Acetophenone isomers Plasma

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Complementary DNA sequencing (cDNA): an eff ective approach for assessing the diversity and distribution of marine benthic ciliates along hydrographic gradients

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Journal of Oceanology and Limnology 2021年第1期39卷 208-222页

作者： Pingping HUANG Feng ZHAO Kuidong XU Laboratory of marine Organism Taxonomy and Phylogeny Institute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for marine biology and Biotechnology Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China University of Chinese Academy of Sciences Beijing 100049China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China

The Yellow Sea Cold Water Mass(YSCWM)is a distinct hydrographic phenomenon of the Yellow Sea,and the distribution pattern of meio-and macrobenthos diff ers inside and outside of the ***,such a pattern has never been observed in the microbenthic ciliate ***,we hypothesized that benthic ciliates followed a similar distribution pattern as meio-and macrobenthos,but this pattern has not been uncovered by morphological *** evaluated the diversity and distribution of benthic ciliates at fi ve stations along hydrographic gradients across the YSCWM and adjacent shallow water by using morphology and DNA and complementary DNA(cDNA)high-throughput sequencing of the v4 region of 18S rRNA *** showed that the diversity of benthic ciliates detected by DNA(303 OTUs),and the cDNA(611 OTUs)sequencing was much higher than that detected by the morphological method(79 species).Morphological method detected roughly diff erent ciliate communities inside and outside of the YSCWM,but without statistical signifi *** clear pattern was obtained by DNA *** contrast,cDNA sequencing revealed a distinct distribution pattern of benthic ciliate communities like meioand macrobenthos,which coincided well with the results of the environmental parameter *** than half of the total sequences detected by DNA sequencing belonged to planktonic ciliates,most(if not all)of which were recovered from historic DNA originating through the sedimentation of pelagic forms because none of them were observed *** irrelevant historic DNA greatly infl uenced the recovery of rare species and thus limited the understanding of the benthic ciliate diversity and *** research indicates that the methods used have signifi cant eff ects on the investigation of benthic ciliate communities and highlights that cDNA sequencing has great advantages in estimating the diversity and distribution of benthic ciliates,as well as the potential for

关键词： benthic ciliates cDNA high-throughput sequencing community comparison DNA highthroughput sequencing morphology

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