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Quantitative proteomic determination of diethylstilbestrol action on prostate cancer

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Asian Journal of Andrology 2013年第3期15卷 413-420页

作者： Pierre Bigot Kevin Mouzat Muriel Bahut Nora Benhabiles Geraldine Cancel Tassin Abdel-Rahmene Azzouzi Olivier Cussenot Department of Urology Angers University Hospital Angers 49933 France CeRePP Tenon Hospital Paris 75970 France Department of Biochemistry N'imes University Hospital NTmes 30029 France Plate-Forme Technologique de Biotechnologie Moleculaire Angers University Angers 49933 France 5cea DSV IRCM SREIT Laboratoire de Cancerologie Experimentale Fontenay-aux-Roses 92265 France

Diethylstilbestrol （DES） has a direct cellular mechanism inhibition on prostate cancer. Its action is independent from the oestrogen receptors and is preserved after a first-line hormonal therapy. We aimed to identify proteins involved in the direct cellular inhibition effects of DES on prostate cancer. We used a clonogenic assay to establish the median lethal concentration of DES on 22RV1 cells. 22RV1 cells were exposed to standard and DES-enriched medium. After extraction, protein expression levels were obtained by two-dimensional differential in-gel electrophoresis （2D-DIGE） and isotope labelling tags for relative and absolute quantification （iTRAQ）. Proteins of interest were analysed by quantitative RT-PCR and western blotting. The differentially regulated proteins （P〈0.01） were interrogated against a global molecular network based on the ingenuity knowledge base. The 2D-DIGE analyses revealed DES-induced expression changes for 14 proteins （〉 1.3 fold; P〈0.05）. The iTRAQ analyses allowed the identification of 895 proteins. Among these proteins, 65 had a modified expression due to DES exposure （i.e., 23 overexpressed and 42 underexpressed）. Most of these proteins were implicated in apoptosis and redox processes and had a predicted mitochondrial expression. Additionally, ingenuity pathway analysis placed the OAT and HSBP1 genes at the centre of a highly significant network. RT-PCR confirmed the overexpression of OAT （P=0.006） and HSPB1 （P=0.046）.

关键词： cultured cells DES diethylstilbestrol ingenuity pathway analysis isotope labelling mass spectrometry prostate cancer proteomics

Gradient‑Layered MXene/Hollow Lignin Nanospheres Architecture Design for Flexible and Stretchable Supercapacitors

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Nano-Micro Letters 2025年第2期17卷 447-462页

作者： Haonan Zhang Cheng Hao Tongtong Fu Dian Yu Jane Howe Kaiwen Chen Ning Yan Hao Ren Huamin Zhai Jiangsu Provincial Key Lab of Sustainable Pulp and Paper Technology and Biomass Materials NanJing Forestry UniversityNanjing 210037People’s Republic of China Department of Chemical Engineering and Applied Chemistry University of Toronto200 College StreetTorontoON M5S 3E5Canada Department of Materials Science and Engineering University of Toronto184 College StreetTorontoON M5S 3E4Canada College of Materials Science and Engineering Nanjing Forestry UniversityNanjing 210037People’s Republic of China

With the rapid development of flexible wearable electronics,the demand for stretchable energy storage devices has *** this work,a novel gradient-layered architecture was design based on single-pore hollow lignin nanospheres(HLNPs)-intercalated two-dimensional transition metal carbide(Ti_(3)C_(2)T_(x) MXene)for fabricating highly stretchable and durable *** depositing and inserting HLNPs in the MXene layers with a bottom-up decreasing gradient,a multilayered porous MXene structure with smooth ion channels was constructed by reducing the overstacking of MXene ***,the micro-chamber architecture of thin-walled lignin nanospheres effectively extended the contact area between lignin and MXene to improve ion and electron accessibility,thus better utilizing the pseudocapacitive property of *** these strategies effectively enhanced the capacitive performance of the *** addition,HLNPs,which acted as a protective phase for MXene layer,enhanced mechanical properties of the wrinkled stretchable electrodes by releasing stress through slip and deformation during the stretch-release cycling and greatly improved the structural integrity and capacitive stability of the *** electrodes and symmetric flexible all-solid-state supercapacitors capable of enduring 600%uniaxial tensile strain were developed with high specific capacitances of 1273 mF cm^(−2)(241 F g^(−1))and 514 mF cm^(−2)(95 F g^(−1)),***,their capacitances were well preserved after 1000 times of 600%stretch-release *** study showcased new possibilities of incorporating biobased lignin nanospheres in energy storage devices to fabricate stretchable devices leveraging synergies among various two-dimensional nanomaterials.

关键词： Hollow lignin nanospheres MXene Gradient-layered architecture Wrinkled electrodes Stretchable supercapacitors

Characteristics of Late-Quaternary Activity and Seismic Risk of the Northeastern Section of the Longmenshan Fault Zone

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Acta Geologica Sinica(English Edition) 2013年第6期87卷 1674-1689页

作者： WANG Mingming ZHOU Bengang YANG Xiaoping XIE Chao GAO Xianglin Key Laboratory of Active Tectonics and Volcano Institute of Geology CEA Sichuan Seistech Corporation Ltd. Key Laboratory of Earthquake Prediction Institute of Earthquake Science CEA Institute of Geology CEA

Following the 2008 Wenchuan M8 earthquake,the seismic risk of the northeastern section of the Longmenshan fault zone and the adjacent Hanzhong basin has become an issue that receives much *** is facing,however,the problem of a lack of sufficient data because of little previous work in these *** northeastern section of the Longmenshan fault zone includes three major faults：the Qingchuan fault,Chaba-Lin＇ansi fault,and Liangshan south margin fault,with the Hanzhong basin at the northern *** paper presents investigations of the geometry,motion nature,and activity ages of these three faults,and reveals that they are strike slip with normal faulting,with latest activity in the Late *** implies that this section of the Longmenshan fault zone has been in an extensional setting,probably associated with the influence of the Hanzhong *** analysis of the tectonic relationship between the Longmenshan fault zone and the Hanzhong basin,this work verifies that the Qingchuan fault played an important role in the evolution of the Hanzhong basin,and further studies the evolution model of this ***,with consideration of the tectonic setting of the Longmenshan fault zone and the Hanzhong basin as well as seismicity of surrounding areas,this work suggests that this region has no tectonic conditions for great earthquakes and only potential strong events in the future.

关键词： Seismic risk northeastern Longmenshan fault zone Hanzhong basin Late-Quaternary activity

Mercury indicating inflow zones and ruptures along the Wenchuan Ms 8.0 earthquake fault

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Chinese Journal Of Geochemistry 2015年第2期34卷 201-207页

作者： Yang Duoxing Zhang Lei Liu Yaowei Ren Hongwei Xie Furen Chen Gangcai Key Laboratory of Crustal Dynamics Institute of Crustal DynamicsCEA Institute of Geophysics CEA Chongqing Academy of Environmental Sciences

During the Wenchuan Fault Scientific Drilling Project,we determined the values of total mercury(HgT)and gaseous elemental mercury(GEM) from drilled cores and drilling mud,*** analysis shows HgT values ranging from 0.24 to 6.45 ng/g for the Penguan complex and from 2.90 to 137.54 ng/g for T3 *** average levels of HgT for the Penguan complex and T3 sediments are 1.81 ± 0.26 ng/g and23.96 ± 4.80 ng/g,*** anomalous peaks of HgT appear at depth of 614,731,993 and 1,107 m,which correspond to the long-term high seismic activity during crustal deformation in response to tectonic *** elemental mercury dissolved in drilling mud was also *** found fluid inflow zones with high GEM at depths of 590-750 m,suggesting that fluid-filled ruptures exist in the LMS fault *** indicates that mercury provides geochemical evidence for inflow zones and ruptures/fault zones in the Wenchuan Ms 8.0 earthquake fault.

关键词：地震断层断裂带汶川水星明流科学钻探工程地球化学分析钻井泥浆

Looking into the future of Nanoelectronics in the Diversification Efficient Era

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Science China(Information Sciences) 2016年第6期59卷 6-19页

作者： Simon DELEONIBUS Universit′es Grenoble Alpes CEA LETIMINATEC Campus

The linear scaling of CMOS has encountered, since its beginning, many hurdles which request new process modules, driven mainly by the maximization of energy efficiency. Fabrication at the sub 10 nm node level will request Intrinsic Variability approaching to zero. The rapid growth of mobile, multifunctional and autonomous systems is hardly demanding to reach Zero Power consumption. The solutions to integrate Thin Film based devices, architectures and systems in order to face these challenges are described.

关键词： CMOS thin films heterogeneous integration NEMS 3D integration sequential parallel zero intrinsic variability zero power systems

A unified electrical model based on experimental data to describe electrical transport in carbon nanotube-based materials

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Nano Research 2020年第6期13卷 1764-1779页

作者： Yoann Dini Jérôme Faure-Vincent Jean Dijon Univ.Grenoble-Alpes CEALITENDTNMF-38000GrenobleFrance Univ.Grenoble-Alpes CEACNRSIRIGSyMMESF-38000GrenobleFrance

Understanding the electrical transport in carbon nanotube(CNT)materials is one key to reach very high electrical *** CNT material resistivity(ρ(T))as function of the temperature are fully apprehended by their reduced activation W(T)=dln(ρ)/dln(T)*** to now,no model accurately fits W(T)curves,thus preventing from precisely describing the CNT material electrical *** present a new electrical transport model that perfectly fits all W(T)curves found in the literature and in our own *** material resistivities are modeled byρ(T)=ρ0(T^−α+M(1+βTγT^2)).Our model has few enough parameters(α,M,β,γ)to relate them to the CNT *** 70 K,we experimentally show that CNT material resistivity follows the Luttinger Liquid theory justifying the T^−αterm in our *** 70 K,the polynomial part becomes dominant and depends on the two different CNT fabrication techniques which lead to two very different yarn *** yarns made from floating catalyst chemical vapor deposition CNTs,the polynomial is explained by the percolation of metallic CNT ***,the polynomial of yarns spun from CNT arrays is explained by the electrical transport in CNT bundles which are the basic building blocks of this type of yarns.

关键词： carbon nanotube carbon-based materials electrical conductivity electrical transport model Luttinger Liquid

Late Pleistocene-Holocene slip history of the Langshan-Seertengshan piedmont fault(Inner Mongolia,northern China) from cosmogenic ^(10)Be dating on a bedrock fault scarp

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Journal of Mountain Science 2016年第5期13卷 882-890页

作者： SHEN Xiao-ming LI De-wen TIAN Yun-tao LV Yan-wu LI Da-wei LI Yan-feng Key Laboratory of Crustal Dynamics Institute of Crustal DynamicsCEA Department of Earth Sciences University College London National Earthquake Response Support Service CEA

Offset geomorphic features and deformed late Quaternary strata indicate active deformation along the Langshan-Seertengshan piedmont fault （LSPF）, one of the most active faults in the Hetao fault zone in Inner Mongolia, North China. The widespread occurrence of bedrock fault scarps along the LSPF offers excellent opportunity to examine the faulting history. Using cosmogenic ^10Be exposure dating, we measured the exposure ages of the western Langshankou scarp, located in the middle segment of the LSPF. Our data revealed at least two earthquakes that occurred at 22.2±3.3 Ira and 7.2±2.4 ka, respectively. These events are consistent with previous paleoseismic trench studies. The regression of the relationship between the age and sampling height along the scarp yield a fault slip rate of 0.10 ±0.05/-0.06 mm/yr, which is significantly lower than the average post-late Pleistocene fault slip rate of ~1 mm/yr, as estimated from the offset of the T2 terraces by previous studies. This indicates that the slip of the LSPF may have been accommodated by other fault branches.

关键词： Lanshan-Seertengshan piedmont fault(LSPF) Fault scarp Cosmogenic nuclide Exposureage Fault slip rate

A Node-Centered Artificial Viscosity Method for Two-Dimensional Lagrangian Hydrodynamics Calculations on a Staggered Grid

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Communications in Computational Physics 2010年第9期8卷 877-900页

作者： A.Burbeau-Augoula cea DIFF-91297 ArpajonFrance

This work deals with the simulation of two-dimensional Lagrangian hydrodynamics *** objective is the development of an artificial viscosity that is to be used in conjunction with a staggered placement of variables:thermodynamics variables are centered within cells and position and fluid velocity at ***[***.,228(2009),2391-2425],Maire develops a high-order cell-centered scheme for solving the gas dynamics *** numerical results show the accuracy and the robustness of the method,and the fact that very few Hourglass-type deformations are *** objective is to establish the link between the scheme of Maire and the introduction of artificial viscosity in a Lagrangian code based on a staggered *** idea is to add an extra degree of freedom to the numerical scheme,which is an approximation of the fluid velocity within *** that,we can locally come down to a cell-centered approximation and define the Riemann problem associated to discrete variable discontinuities in a very natural *** results in a node-centered artificial viscosity *** experiments show the robustness and the accuracy of the method,which is very easy to implement.

关键词： Hydrodynamics staggered discretization artificial viscosity Lagrangian simulation shock waves finite volume scheme

Chloroplast Proteomics and the Compartmentation of Plastidial Isoprenoid Biosynthetic Pathways

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Molecular Plant 2009年第6期2卷 1154-1180页

作者： Jacques Joyard Myriam Ferro Christophe Masselon Daphne Seigneurin-Berny Daniel Salvi Jerome Garin Norbert Rolland Laboratoire de Physiologie Cellulaire Vegetale UMR 5168 cea CNRS INRA Universite Joseph Fourier iRTSV CEA-Grenoble 38054 Grenoble-cedex 9 France Laboratoirre Etude de la Dynamique des Proteomes U880 cea INSERM Universite Joseph Fourrier iRTSV CEA-Grenoble 38054 Grenoble-cedex 9 France

Recent advances in the proteomic field have allowed high-throughput experiments to be conducted on chloroplast samples. Many proteomic investigations have focused on either whole chloroplast or sub-plastidial fractions. To date, the Plant Protein Database （PPDB, Sun et al., 2009） presents the most exhaustive chloroplast proteome available online. However, the accurate localization of many proteins that were identified in different sub-plastidial compartments remains hypothetical. Ferro et al. （2009） went a step further into the knowledge of Arabidopsis thaliana chloroplast proteins with regards to their accurate localization within the chloroplast by using a semi-quantitative proteomic approach known as spectral counting. Their proteomic strategy was based on the accurate mass and time tags （AMT） database approach and they built up AT_CHLORO, a comprehensive chloroplast proteome database with sub-plastidial localization and curated information on envelope proteins. Comparing these two extensive databases, we focus here on about 100 enzymes involved in the synthesis of chloroplast-specific isoprenoids. Well known pathways （i.e. compartmentation of the methyl erythritol phosphate biosynthetic pathway, of tetrapyrroles and chlorophyll biosynthesis and breakdown within chloroplasts） validate the spectral counting-based strategy. The same strategy was then used to identify the precise localization of the biosynthesis of carotenoids and prenylquinones within chloroplasts （i.e. in envelope membranes, stroma, and/or thylakoids） that remains unclear until now.

关键词： Non-mevalonate pathway chlorophyll biosynthesis chlorophyll degradation carotenoid biosynthesis prenylquinone biosynthesis envelope stroma thylakoids.