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HSV-2-encoded miRNA-H4 Regulates Cell Cycle Progression and Act-Dinduced Apoptosis in HeLa Cells by Targeting CDKL2 and CDKN2A

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Virologica Sinica 2019年第3期34卷 278-286页

作者： Yang Zhao Jingjing Yang Yan Liu Jianyong Fan Huilan Yang Guangzhou School of Clinical Medicine Southern Medical UniversityGuangzhou 510010China Department of Dermatology General Hospital of Southern Theatre Command of PLAGuangzhou 510010China

MicroRNAs(miRNAs)encoded by latency-associated transcript are associated with both latent and acute stages of herpes simplex virus 2(HSV-2)*** this study,miRNA-H4-5p and miRNA-H4-3p were ectopically expressed in HeLa cells to explore potential cellular targets of viral miRNAs and demonstrate their potential biological *** results showed that miRNA-H4-5p could reverse apoptosis induced by actinomycin D(Act-D)and promote cell cycle progression,but miRNA-H4-3p had no such obvious *** analysis,luciferase report assay,quantitative reverse transcription polymerase chain reaction(qRT-PCR),and Western blotting demonstrated that miRNA-H4-5p could bind to the 3-′untranslated region(UTR)of cyclin-dependent kinase inhibitor 2A(CDKN2A)and cyclin-dependent kinase-like 2(CDKL2)to negatively regulate their *** verified that these two targeted genes were associated with cell apoptosis and cell ***,in HeLa cells infected with HSV-2,we detected significantly reduced expression of CDKN2A and CDKL2 and demonstrated the negative regulation effect of miRNA-H4-5p on these two target *** findings show that viral miRNAs play a vital role in regulating the expression of the host's cellular genes that participate in cell apoptosis and progression to reshape the cellular environment in response to HSV-2 infection,providing further information on the roles of encoded herpesvirus miRNAs in pathogen-host interaction.

关键词： Herpes simplex virus 2(HSV-2) MicroRNAs(miRNAs) Anti-apoptosis-Cell cycle progression Pathogen-host interaction

Ethical issues in penile transplantation

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Asian Journal of Andrology 2010年第6期12卷 795-800页

作者： Li-Chao Zhang Yong-Bing Zhao Wei-Lie Hu Department of Urology Guangzhou General Hospital of Guangzhou Military Command Guangzhou 510010 China

This article provides an overview of the ethical issues associated with penile transplantation, a form of composite tissue allografting. There is only one reported case of human penile transplantation, and, as such, this technique is considered to be experimental. The ethical issues at stake involve both the graft donor and the graft recipient. With regard to the recipient, there are significant concerns relating to surgical risks and benefits, informed consent, body image （including surgical expectations and outcomes） and compliance. Donor issues may include family consent and privacy, as well as graft harvesting （leaving the donor cadaver without a penis）. Many of these ethical issues can be explored during the recipient＇s assessment and consent process. Because no medium-term or long-term outcome data for this procedure exist---only one such operation has ever been performed--the burdens and ethical issues concerning penile transplantation remain unknown.

关键词： ethics penis transplantation

枢椎支点螺钉在寰枢椎钉棒固定中复位能力的生物力学测试

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中国组织工程研究 2025年第15期29卷 3116-3120页

作者：陈树金马向阳邹小宝廖英强漆海如刘宝曾宪明中山火炬开发区人民医院脊柱外科广东省中山市528436 中国人民解放军南部战区总医院脊柱外科广东省广州市510010

背景:目前,寰枢椎脱位的手术治疗主要采取后路寰枢椎钉棒内固定系统进行提拉复位,术中常通过增大连接棒的弯曲度进而增加寰椎和枢椎间的落差来提升复位效果,但增加了手术难度及风险。枢椎支点螺钉直接增加了寰椎和枢椎间的复位落差,但... 详细信息

背景:目前,寰枢椎脱位的手术治疗主要采取后路寰枢椎钉棒内固定系统进行提拉复位,术中常通过增大连接棒的弯曲度进而增加寰椎和枢椎间的落差来提升复位效果,但增加了手术难度及风险。枢椎支点螺钉直接增加了寰椎和枢椎间的复位落差,但其增加复位能力的程度尚不清楚。目的:测试枢椎支点螺钉的复位能力,并与普通螺钉进行对比。方法:选取6具新鲜人体颅颈标本,切除双侧侧块关节囊、寰齿关节囊及横韧带制成寰枢椎失稳模型,每具标本枢椎双侧均依次进行3种内固定:植入单轴枢椎支点螺钉(A组)、多轴枢椎支点螺钉(B组)及普通螺钉(C组),将柔性超薄式薄膜压力传感器置于寰齿前间隙,置入2根相同曲度连接棒,模拟提拉复位,获取寰齿前间隙压力,3组间进行对比分析。结果与结论:①A-C组标本寰齿前间隙压力分别为(97.59±8.58),(60.43±5.09),(22.74±0.81)N,3组间寰齿前间隙压力比较差异有显著性意义(F=251.603,P=0.000),3组间两两比较差异有显著性意义(P=0.000);②结果显示相较于普通颈椎后路螺钉,枢椎支点螺钉应用于后路寰枢椎钉棒内固定系统可提升复位能力,且单轴枢椎支点螺钉相较于多轴枢椎支点螺钉提升后路寰枢椎钉棒内固定系统的复位能力更佳。

关键词：枢椎支点螺钉寰枢椎钉棒内固定系统寰枢椎脱位复位能力生物力学

尖锐湿疣患者皮损中人乳头瘤病毒核酸检测分析

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中华皮肤科杂志 1992年第2期25卷 77-79页

作者：赖晃文王达富 510010 510010

应用HPV6b,HPV11,HPV16和HPV18型DNA探针,对48例生殖器尖锐湿疣和15例正常宫颈组织进行核酸序列检测.结果:尖锐湿疣HPY各型总阳性例数为42例(87.5%),而正常宫颈组织HPV各型均阴性,尖锐湿疣HPV6b,11,16,18型探针检出率分别为68.8%,79.2%,... 详细信息

应用HPV6b,HPV11,HPV16和HPV18型DNA探针,对48例生殖器尖锐湿疣和15例正常宫颈组织进行核酸序列检测.结果:尖锐湿疣HPY各型总阳性例数为42例(87.5%),而正常宫颈组织HPV各型均阴性,尖锐湿疣HPV6b,11,16,18型探针检出率分别为68.8%,79.2%,43.8%,14.6%,提示本组病例以HPV11和HPV6b型感染为主.男女性患者各型探针阳性率基本相近.并发现本组病例以二型以上混合感染多见.

关键词：乳头状瘤病毒尖锐湿疣 DNA探针

Study of Benign and Malignant Intraductal Lesions of the Breast by Fiberoptic Ductoscopy

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Chinese Journal of Clinical Oncology 2005年第1期2卷 529-532页

作者： JuanXu QiWang AnqinZhang WenpingLi JuntaoShi ZhongyangChen BreastCenteroftheGuangdongMatemalandChildren＇sHospital GuangzhouMedicalCollegeGuangzhou510010China BreastCenteroftheGuangdongMatemalandChildren＇sHospital GuangzhouMedicalCollegeGuangzhou510010China

OBJECTIVE To observe and subtype the appearance of intraductal papilloma (lesions) and of infiltrating ductal carcinoma or early infiltrating ductal carcinoma using a fiberoptic ductoscope (FDS) examination, and to discuss the differentiation and diagnosis of benign and malignant tumors,by *** The characteristics of FDS images and diagnostic data for 229 patients with intraductal papilloma (lesions) and 50 patients with ductal carcinoma, who were confirmed by surgical pathology from October 1998 to December 2003, were analyzed *** The appearance of the lesions observed by FDS were grouped into 4types: a monothelia (type Ⅰ), polythelia (type Ⅱ), superficies (type Ⅲ) and a mixture (type Ⅳ). Intraductal papillomas (lesions) were more commonly seen in type Ⅰ and Ⅱ, and intraductal carcinomas, or early infiltrating ductal carcinomas were more commonly seen in type Ⅲ and Ⅳ;there was a statistically significant difference in the distribution of the ductoscopic types, except in type Ⅱ, between the two types of lesions, P<0.001. The focal detection rate by FDS for intraductal papilloma and papillomatosis was 99.6% (228/229) and for breast cancer was 96.0% (48/50). The diagnostic accuracy was 97.8% (224/229) and 82.0% (41/50),*** FDS can be a guide for the treament of benign and malignant intraductal tumors, with early discovery and accurate diagnosis.

关键词：管内乳突淋瘤胸腺检查诊断方法纤维支气管镜

Effects of Different Surfactant Administrations on Cerebral Autoregulation in Preterm Infants with Respiratory Distress Syndrome

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2016年第6期36卷 801-805页

作者： Xu-fang LI Ting-ting CHENG Rui-lian GUAN Hong LIANG Wei-neng LU Jing-hua ZHANG Mei-yi LIU Xin YU Jun LIANG Li SUN Lian ZHANG Department of Neonatology Guangzhou Women and Children k Medical Center Guangzhou 510010 China

To treat respiratory distress syndrome,surfactant is currently delivered via less invasive surfactant administration（LISA） or INtubation SURfactant Extubation（INSURE）.The aim of this study was to compare the effect of the two delivery methods of surfactant on cerebral *** infrared spectroscopy monitoring was carried out to detect cerebral oxygen saturation（Sc O2）,and the mean arterial blood pressure（MABP） was simultaneously *** 44 preterm infants included,the surfactant was administrated to 22 via LISA and 22 via *** clinical characteristics,treatments and outcomes of the infants showed no significant differences between the two *** correlation coefficient of Sc O2 and MABP（rSc O2-MABP） 5 min before administration was similar in the two *** surfactant administration,rSc O2-MABP increased in both groups（0.44±0.10 to 0.54±0.12 in LISA,0.45±0.11 to 0.69±0.09 in INSURE）.In the first and second 5 min after instillation,rSc O2-MABP was not significantly different from baseline in the LISA group,but increased in the first 5 min after instillation（0.59±0.13,P=0.000 compared with the baseline in the same group） and recovered in the second 5 min after instillation（0.48±0.10,P=0.321） in the INSURE *** were significant differences in the change rates of rSc O2-MABP between the two groups during and after surfactant *** results suggest that cerebral autoregulation may be affected transiently by surfactant *** effect duration of LISA is shorter than that of INSURE（〈5 min in LISA vs.5–10 min in INSURE）.

关键词： preterm infant respiratory distress syndrome surfactant cerebral autoreguiation pulmonary surfactant

Design and application of 60mer oligonucleotide microarray in SARS coronavirus detection

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Chinese Science Bulletin 2003年第12期48卷 1165-1169页

作者： SHIRong MAWenli WUQinghua ZHANGBao SONGYanbin GUOQiuye XIAOWeiwei WANGYan ZHENGWenling InstituteofMolecularBiology FirstMilitaryMedicalUniversityGuangzhou510515China DepartmentofMedicalResearch GuangzhouLiuHuaQiaoHospitalGuangzhou510010China

The 60mer oligonucleotide microarray was designed and applied to detecting of SARS (severe acute respiratory syndrome) coronavirus. Thirty 60mer specific oligos were designed to cover the whole genome of the first submitted coronavirus strain, according to the sequence of TOR2 (GENEBANK Accession: AY274119). These primers were synthesized and printed into a microarray with 12×12 spots. RNAs were extracted from the throat swab and gargling fluid of SARS patients and reverse-transcripted into the double strand cDNAs. The cDNAs were prepared as restricted cDNA fragments by the restriction display (RD) technique and labeled by PCR with the Cy5-universal primer. The labeled samples were then applied to the oligo microarray for hybridization. The diagnostic capability of the microarray was evaluated after the washing and scanning steps. The scanning result showed that samples of SARS patients were hybridized with multiple SARS probes on the microarray, and there is no signal on the negative and blank controls. These results indicate that the genome of SARS coronavirus can be detected in parallel by the 60mer oligonucleotide microarray, which can improve the positive ratio of the diagnosis. The oligo microarray can also be used for monitoring the behavior of the virus genes in different stages of the disease status.

关键词：寡核苷酸微队列 SARS 冠状病毒检测 RD技术荧光标记分子杂交

Establishment of an in vitro cell culture system transfected by full-length HCV cDNA genome

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Chinese Science Bulletin 2004年第13期49卷 1358-1363页

作者： YAOXiangjie GUOJia ZHENGCongyi FANGChengxiang QUSanfu CHENZhenqiu LIZhonghong LIXinqiang LIWeiyun MinistryofEducationKeyLaboratoryofVirology CollegeofLifeSciencesWuhanUniversityWuhan430072China ChinaWaveBiotechnologyCo.Ltd. Guangzhou510010China

A new cell culture system expressing the entire HCV geuome has been established in vitro. To initiate transcription of HCV RNA, HeLa cells were trausfected with arecombinant plasmid containing full-length HCV cDNA geuome by using lipofectamiue 2000, followed by infection with recombinant vacciuia virus vTF7-3 containing the T7 RNA polymerase geue. Synthesis of positive-strand HCV RNA could be detected in the trausfected cells by RT-PCR. Western blot analysis revealed that HCV structural and nonstructural proteins were correctly processed. In trausfected HeLa cells 47 um virus-like particles were assembled, whichcould be recognized by auti-HCV E2 antibodies. The titer of HCV was 107 copies/mL in our cell culture system, which was significantly higher than that of infected patients' sera and that from all reported cell culture systems. Superuataut from trausfected HeLa cells were infectious to Huh7 cells and thetiter of HCV was 106 copies/mL. Moreover, negative-strand RNA of HCV in Huh7 cells could be detected by using strand-specific RT-PCR, which demonstrated that replication of HCV occurred in the permissive cell lines.

关键词： HCV 体外细胞培养系统 cDNA 肝炎病毒传染病重组细胞种痘

Three core techniques in surgery of neuroepithelial tumors in eloquent areas： awake anaesthesia, intraoperative direct electrical stimulation and ultrasonography

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Chinese Medical Journal 2011年第19期000卷 3035-3041页

作者： BAI Hong-min WANG Wei-min LI Tian-dong HE Huan SHI Chong GUO Xiao-fei LIU Yan WANG Li-min WANG Sha-sha Department of Neurosurgery Liuhuaqiao Hospital Guangzhou Guangdong 510010 China Department of Anaesthesiology Liuhuaqiao Hospital Guangzhou Guangdong 510010 China Department of Ultrasound Imaging Liuhuaqiao Hospital Guangzhou Guangdong 510010 China

Background The goal of surgery in the treatment of intrinsic cerebral tumors is to resect the maximum tumor volume,and to spare the eloquent areas. However, it is difficult to discover the eloquent areas intraoperatively due to individual anatomo-functional variability both for sensori-motor and language functions. Consequently, the surgery of intrinsic cerebral tumors frequently results in poor extent of resection or permanent postoperative deficits, or both, and remains a difficult problem for *** From January 2003 to January 2010, 112 patients with neuroepithelial tumors in/close to the eloquent areas were operated on under awake anesthesia with the intraoperative direct electrical stimulation for functional mapping of the eloquent areas. The extent of the tumors was verified by intraoperative ultrasonography. The maximal resection of the tumors and minimal damage of the eloquent areas were the surgical goal of all *** Totally 356 cortical sites in 99 patients were detected for motor response by intraoperative direct electrical stimulation, 50 sites in 16 patients for sensory, 72 sites in 48 patients for language. Sixty-six patients （58.9%） achieved total resection, 34 （30.4%） subtotal and 12 （10.7%） partial. Fifty-eight patients （51.8%） had no postoperative deficit, while 37 patients （33.0%） had transitory postoperative paralysis, 26 patients （23.2%） with transitory postoperative language disturbance and 3 patients （2.7%） with permanent neurological deficits. No patient complained of pain recollection following *** Awake anesthesia, intraoperative direct electrical stimulation and ultrasonography are three core techniques for the resection of intrinsic cerebral tumors near the eloquent areas. This new concept allows an improvement in the quality of surgery for neuroepithelial tumors in/adjacent to eloquent areas.

关键词： neuroepithelial tumors surgery direct electrical stimulation awake anaesthesia ultrasonography