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piR-7472影响电压调控钾离子通道促进小鼠成骨细胞分化的机制

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中国组织工程研究 2025年第14期29卷 2868-2874页

作者：隆宇斌王湘斌樊基耿杨厚志杨洋李勇邵阳市中心医院脊柱外科湖南省邵阳市422000 天津医科大学研究生院天津市300070 邵阳市中心医院肿瘤内科湖南省邵阳市422000

背景:现有研究在PIWI相互作用RNA(piRNA)抗骨质疏松方面取得了重大进展,但piRNA发挥功能的具体靶点和相关机制仍有待探索。目的:探讨piR-7472对小鼠成骨细胞(MC3T3-E1细胞)分化的影响及下游机制。方法:①12只C57/BL6J小鼠随机分为假手... 详细信息

背景:现有研究在PIWI相互作用RNA(piRNA)抗骨质疏松方面取得了重大进展,但piRNA发挥功能的具体靶点和相关机制仍有待探索。目的:探讨piR-7472对小鼠成骨细胞(MC3T3-E1细胞)分化的影响及下游机制。方法:①12只C57/BL6J小鼠随机分为假手术组和卵巢切除组,每组6只,术后8周通过Micro-CT检测小鼠的骨量,RT-qPCR检测piR-7472的表达;②将MC3T3-E1细胞分为NC mimics组、piR-7472 mimics组、NC inhibitor组与piR-7472 inhibitor组,转染后成骨诱导7 d采用RT-qPCR检测piR-7472、骨桥蛋白、胶原蛋白Ⅰ、Runt相关转录因子2、钾电压门控通道调节因子亚家族F成员1 mRNA的表达;成骨诱导7 d采用Western blot检测骨桥蛋白、Runt相关转录因子2、骨形态发生蛋白2、钾电压门控通道调节因子亚家族F成员1(KCNF1)蛋白表达;成骨诱导14 d通过碱性磷酸酶染色检测碱性磷酸酶的表达;成骨诱导21 d通过茜素红染色检测矿化结节数量;③双荧光素酶报告基因实验观察piR-7472是否能与钾电压门控通道调节因子亚家族F成员1相结合。结果与结论:①与假手术组相比,卵巢切除组小鼠骨密度、骨体积分数、骨小梁厚度、骨小梁数量明显下降,骨小梁分离度明显升高,并且骨组织中piR-7472 mRNA表达明显下调;②与NC组相比,piR-7472 mimics组骨桥蛋白、胶原蛋白Ⅰ、Runt相关转录因子2的mRNA表达明显升高,骨桥蛋白、Runt相关转录因子2、骨形态发生蛋白2的蛋白表达明显升高,矿化沉积与碱性磷酸酶表达增多;与NC inhibitor组相比,piR-7472 inhibitor组骨桥蛋白、胶原蛋白Ⅰ、Runt相关转录因子2的mRNA表达明显降低,骨桥蛋白、Runt相关转录因子2、骨形态发生蛋白2的蛋白表达明显降低,矿化沉积与碱性磷酸酶表达降低;③通过miRanda数据库预测发现piR-7472能与KCNF1相互作用;双荧光素酶报告基因实验发现piR-7472 mimcis能与钾电压门控通道调节因子亚家族F成员1结合并促进其表达。结果表明:piR-7472能够促进成骨前体细胞MC3T3-E1成骨分化,其发挥作用的机制可能是通过促进KCNF1表达实现的。

关键词：成骨细胞MC3T3-E1 PIWI相互作用RNA 成骨分化骨质疏松症电压调控的钾离子通道 KCNF1

Global Attractivity of Positive Periodic Solutions for a Survival Model of Red Blood Cells

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Acta Mathematicae Applicatae Sinica 2007年第3期23卷 523-528页

作者： Xin-min Wu Jing-wen Li Department of Mathematics Shaoyang University Shaoyang 422000 China

In this paper, we deal with a model for the survival of red blood cells with periodic coefficients x＇（g）=-μ（t）x（t）＋P（t）e^-γ（t）x（t-τ（t））,t≥0.（＊）A new sufficient condition for global attractivity of positive periodic solutions of Eq. （＊） is obtained. Our criterion improves corresponding result obtained by Li and Wang in 2005.

关键词： A survival model of red blood cells positive periodic solution global attractirvity positiveequilibrium

Polygonatum sibiricum polysaccharides protect against obesity and non-alcoholic fatty liver disease in rats fed a high-fat diet

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Food Science and Human Wellness 2022年第4期11卷 1045-1052页

作者： Li Zeng Fangwei Zhong Zhiliang Chen Gengxi Li Qi Zhu School of Pharmacy Shaoyang UniversityShaoyang 422000China School of Laboratory Medicine Shaoyang UniversityShaoyang 422000China

Polygonatum sibiricum is a traditional medicinal and dietary plant of the family Liliaceae. The main functional macromolecules of P. sibiricum are polysaccharides, which function in antioxidation and regulating immunity. Previous studies have shown that insulin resistance(IR), oxidative stress, and inflammation are important factors in the induction of lipid metabolic diseases such as obesity. Therefore, in this study, we established a high-fat diet-induced rat model of obesity and nonalcoholic fatty liver disease(NAFLD) to explore the potential protective effect of P. sibiricum polysaccharides(PSPs) and the mechanisms behind it. After 4 weeks of high-fat diet feeding to induce obesity, the rats were treated with different doses of PSP solution or distilled water for 6 weeks. Compared with untreated obese rats, PSP-treated obese rats showed a decrease in body weight, serum total cholesterol, triglyceride, and low-density lipoprotein cholesterol levels, hepatic aspartate aminotransferase and alanine aminotransferase activity, hepatic malondialdehyde content, and hepatic levels of the pro-inflammatory factors tumor necrosis factor-α, interleukin-1β, and interleukin-6, as well as increased serum high-density lipoprotein cholesterol levels and hepatic superoxide dismutase, catalase, and glutathione peroxidase activity. Pathological analysis and immunoblotting of the liver tissues indicated that mechanistically, PSPs reduced obesity and NAFLD in rats by upregulating insulin receptor expression, increasing adenosine monophosphate-activated protein kinase phosphorylation, and downregulating sterol regulatory element-binding protein 2 and low-density lipoprotein receptor expression, thus promoting lipid metabolism, decreasing body weight, and reducing inflammation and oxidative stress caused by lipid accumulation. Based on these results, PSPs may have the potential to reduce obesity and NAFLD associated with a high-fat diet.

关键词： Polygonatum sibiricum polysaccharide High-fat diet Obesity Non-alcoholic fatty liver disease Lipid metabolism Rat

Manipulation of a temporal electron-spin splitter via aδ-potential in an embedded magnetic-electric-barrier microstructure

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Communications in Theoretical Physics 2021年第12期73卷 180-185页

作者： Gui-Xiang Liu Ge Tang Jian-Lin Liu Qing-Meng Guo Shuai-Quan Yang Shi-Shi Xie College of Science Shaoyang UniversityShaoyang 422000China

We theoretically explore the manipulation of a temporal electron-spin splitter by aδ-potential in an embedded magnetic-elec tric-barrier micro structure(EMEBM),which is constructed by patterning a ferromagnetic stripe and a Schottky-metal stripe on the top and bottom of an InAs/Al_(x)In_(1-x)As heterostructure,*** polarization of the dwell time remains,even though aδ-potential is inserted by atomic-layer *** the magnitude and sign of the spinpolarized dwell time can be manipulated by changing the weight or position of the ***,a structurally controllable temporal electron-spin splitter can be obtained for spintronics device applications.

关键词： embedded magnetic-electric-barrier microstructure(EMEBM) δ-potential dwell time spin polarization manipulable temporal spin splitter

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阿魏酸对阿尔茨海默病的神经保护作用

生命的化学 2025年第1期45卷 55-64页

作者：刘梦鸽陈斯亮冯玮琪罗静怡王欣欣蔡红艳韩维娜邵阳学院普爱医学院邵阳 422000 山西医科大学基础医学院太原 030000

阿尔茨海默病(Alzheimer’s disease，AD)是老年人最常见的疾病之一，发病率高，严重影响老年人生活质量，属于年龄相关的记忆和思维能力障碍的疾病。AD发病机制复杂，目前临床上尚无统一的结论和有效的治疗方法，因此找到新的治疗药物... 详细信息

阿尔茨海默病(Alzheimer’s disease，AD)是老年人最常见的疾病之一，发病率高，严重影响老年人生活质量，属于年龄相关的记忆和思维能力障碍的疾病。AD发病机制复杂，目前临床上尚无统一的结论和有效的治疗方法，因此找到新的治疗药物是目前有待解决的问题。研究发现，基于多酚饮食方式，可有效预防或减缓AD进展。阿魏酸(ferulic acid，FA)是一种羟基肉桂酸衍生物，属于多酚类化合物，广泛分布于自然界中，尤其是中草药、谷物麸皮和水果中。已知FA具有抗Aβ聚集、抗氧化和抗炎等多种生物作用。FA及其衍生物对AD动物和细胞模型都具有显著的治疗作用，可作为一种新的治疗AD的神经保护策略。本文主要对FA的各种生物作用进行综述，为进一步探寻FA治疗AD的机制提供参考。

关键词：阿尔茨海默病阿魏酸 β-淀粉样蛋白神经保护作用

Generation of Entangled Coherent States in Raman-Coupled Bose-Einstein Condensates

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Chinese Physics Letters 2004年第6期21卷 999-1002页

作者： KUANGLe-Man ZENGAi-Hua KUANGZhen-Hua DepartmentofPhysics HunanNormalUniversityChangsha410081 DepartmentofPhysics ShaoyangUniversityShaoyang422000

A method for producing entangled coherent states (ECSs) for atomic Bose–Einstein condensates (BECs) is presented. The proposed method involves a BEC with three internal states and two classical laser beams in a three-level Lambda configuration. We show how to generate multi-state ECSs through properly manipulating strengths of these interactions and laser detunings. A maximally entangled coherent state is obtained explicitly.

关键词： Laser beams

Dynamic Simulation Analysis of the Working Device of a ZL50 Loader

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Fluid Dynamics & Materials Processing 2020年第4期16卷 699-707页

作者： Guiju Zhang Caiyuan Xiao Key Laboratory of Hunan Province for Efficient Power System and Intelligent Manufacturing Shaoyang422000China College of Mechanical and Energy Engineering Shaoyang UniversityShaoyang422000China

In this study,assuming a certain type of wheel loader as the main objective of the research,the performances of the working device of the loader are investigated on the basis of an in-house *** creating a three-dimensional model of the working device using Solidworks,this model has been imported into the dynamic simulation software ADAMS,and the simulation problem has been completed by adding the relevant constraints and *** load stress curve relating to the main connecting point of the working device has been obtained in the frame work of this approach and it has been shown that the movement characteristics are compatible with(i.e.,they match)the actual working *** present study may be regarded as a theoretical basis for the design and improvement of the working device of a vast category of wheel loaders.

关键词： ADAMS working device solidworks wheel loader dynamic simulation

Full Quantum Theory of Transient-State Electromagnetically Induced Transparency

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Communications in Theoretical Physics 2004年第3X期42卷 407-412页

作者： KUANGLe-Man ZENGAi-Hua KUANGZhen-Hua DepartmentofPhysics HunanNormalUniversityChangsha410081China DepartmentofPhysics ShaoyangUniversityShaoyang422000China

We develop a full quantum theory of transient-state electromagnetically induced transparency (EIT) in thevapor of three-level A-type atoms interacting with probe and coupling lasers. As applications of the full quantum theory,we show that transient-state EIT medium exhibits normal dispersion and find that group velocities of both coupling andprobe lasers are greatly reduced. It is shown that the group velocity of the probe laser in the transient-state EIT case isequal to that in the adiabatic EIT case and that the coupling laser group velocity in the transient-state EIT is generallyless than that in the adiabatic EIT.

关键词：耦合激光探测激光电磁诱导透射全量子理论三层∧型原子相互作用群速度激光物理

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Defence Technology（防务技术） 2021年第2期17卷 531-544页

作者： Gui-ju Zhang Cai-yuan Xiao Olatunj Oladimeji Ojo Key Laboratory of Hunan Province for Efficient Power System and Intelligent Manufacturing Shaoyang422000HunanChina College of Mechanical and Energy Engineering Shaoyang UniversityShaoyang422000HunanChina Department of Industrial and Production Engineering Federal University of Technology AkureAkureNigeria

This paper studies the friction stir spot welding of AA2024-T3/AA7075-T6 Al alloys in the ambient and underwater environments by clarifying the nugget features,microstructure,fracture and mechanical properties of the *** results show that the water-cooling medium exhibits a significant heat absorption capacity in the AA2024-T3/AA7075-T6 welded *** features such as stir zone width,circular imprints,average grain sizes,and angular inter-material hooking are reduced by the watercooling effect in the *** whitish(intercalated structures)bands are formed in the underwater joints while Mg2Si and Al2CuMg precipitates are formed in the ambient and the underwater welded joints *** increase in tool rotational speed(600e1400 rpm)and plunge depth(0.1 e0.5 mm)increases the tensile-shear force of the welded AA2024-T3/AA7075-T6 joints in both the ambient and underwater *** maximum tensile-shear forces of 5900 N and 6700 N were obtained in the ambient and the underwater welds respectively.

关键词： Friction stir spot welding Underwater friction stir spot welding Nugget characterization Microstructure Mechanical properties Fracture