检索结果-南通市图书馆

维普期刊数据库

Construction of millimeter-scale vascularized engineered myocardial tissue using a mixed gel

在线全文

学校读者我要写书评

暂无评论

Regenerative Biomaterials 2024年第3期3卷 56-67页

作者： Ming Ke Wenhui Xu Yansha Hao Feiyang Zheng Guanyuan Yang Yonghong Fan Fangfang Wang Zhiqiang Nie Chuhong Zhu Department of Anatomy Third Military Medical UniversityChongqing 400038China State Key Laboratory of Trauma Burn and Combined InjuryChongqing 400038China Department of Plastic and Aesthetic Surgery Southwest HospitalThird Military Medical UniversityChongqing 400038China Engineering Research Center of Tissue and Organ Regeneration and Manufacturing Ministry of EducationChongqing 400038China

Engineering myocardium has shown great clinal potential for repairing permanent myocardial ***,the lack of perfusing blood vessels and difficulties in preparing a thick-engineered myocardium result in its limited clinical *** prepared a mixed gel containing fibrin(5 mg/ml)and collagen I(0.2 mg/ml)and verified that human umbilical vein endothelial cells(HUVECs)and human-induced pluripotent stem cell-derived cardiomyocytes(hiPSC-CMs)could fom microvascular lumens and myocardial cell clusters by harnessing the low-hardness and hyperelastic characteristics of ***-CMs and HUVECs in the mixed gel formed self-organized cell clusters,which were then cultured in different media using a three-phase *** successfully constructed vascularized engineered myocardial tissue had a spherical structure and final diameter of *** tissue exhibited autonomous beats that occured at a frequency similar to a normal human heart *** internal microvascular lumen could be maintained for 6 weeks and showed good results during preliminary surface re-vascularization in vitro and vascular remodeling in *** summary,we propose a simple method for constructing vascularized engineered myocardial tissue,through phased cultivation that does not rely on high-end manufacturing equipment and cutting-edge preparation *** constructed tissue has potential value for clinical use after preliminary evaluation.

关键词： engineered myocardium vascularized hiPSC-CM HUVEC fibrin

Rainfall-triggered waste dump instability analysis based on surface 3D deformation in physical model test

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Mountain Science 2024年第5期21卷 1549-1563页

作者： LI Hanlin JIN Xiaoguang HE Jie XUE Yunchuan YANG Zhongping School of Civil Engineering Chongqing UniversityChongqing 400038China

Landslide is the second largest natural disaster after earthquake. It is of significance to study the evolution laws and failure mechanism of landslides based on its surface 3D deformation information. Based on the rainfall-triggered waste dump instability model test, we studied the failure mechanisms of the waste dump by integrating surface deformation and internal slope stress and proposed novel parameters for identifying landslide stability. We developed a noncontact measurement device, which can obtain millimeter-level 3D deformation data for surface scene in physical model test;Then we developed the similar materials and established a test model for a waste dump. Based on the failure characteristics of slope surface, internal stress of slope body and displacement contours during the whole process, we divided the slope instability process in model test into four stages: rainfall infiltration and surface erosion, shallow sliding, deep sliding, and overall instability. Based on the obtained surface deformation data, we calculated the volume change during slope instability process and compared it with the point displacement on slope surface. The results showed that the volume change can not only reflect the slow-ultra acceleration process of slope failure, but also fully reflect the above four stages and reduce the fluctuations caused by random factors. Finally, this paper proposed two stability identification parameters: the volume change rate above the slip surface and the relative velocity of volume change rate. According to the calculation of these two parameters in model test, they can be used for study the deformation and failure mechanism of slope stability.

关键词： Waste dump stability Physical model test Surface 3D deformation Stability identification

维普期刊数据库

Recent progress in hair follicle stem cell markers and their regulatory roles

在线全文

学校读者我要写书评

暂无评论

World Journal of Stem Cells 2024年第2期16卷 126-136页

作者： Yi-Zhan Xing Hai-Ying Guo Fei Xiang Yu-Hong Li Department of Cell Biology Army Medical UniversityChongqing 400038China Institute of Burn Research Southwest HospitalArmy Medical UniversityChongqing 400038China

Hair follicle stem cells(HFSCs)in the bulge are a multipotent adult stem cell *** can periodically give rise to new HFs and even regenerate the epidermis and sebaceous glands during wound *** increasing number of biomarkers have been used to isolate,label,and trace HFSCs in recent *** more detailed data from single-cell transcriptomics technology,we mainly focus on the important HFSC molecular markers and their regulatory roles in this review.

关键词： Hair follicle stem cells Bulge Secondary hair germ Marker Single-cell RNA-sequencing

Development of a Decision Aid for Family Surrogate Decision Makers of Critically Ill Patients Requiring Renal Replacement Therapy in ICU:A User-Centered Design for Rapid Prototyping

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Medical Sciences Journal 2024年第2期39卷 91-101页

作者： Miao Zheng Yong-Hui Zhang Ying Cao Chang-Lin Yin Li-Hua Wang Department of Outpatient First Affiliated Hospital of Army Medical UniversityChongqing 400038China Department of Critical Care Medicine First Affiliated Hospital of Army Medical UniversityChongqing 400038China Administration Office First Affiliated Hospital of Army Medical UniversityChongqing 400038China

Objectives Renal replacement therapy(RRT)is increasingly adopted for critically ill patients diagnosed with acute kidney injury,but the optimal time for initiation remains unclear and prognosis is uncertain,leading to medical complexity,ethical conflicts,and decision dilemmas in intensive care unit(ICU)*** study aimed to develop a decision aid(DA)for the family surrogate of critically ill patients to support their engagement in shared decision-making process with *** Development of DA employed a systematic process with user-centered design(UCD)principle,which included:(i)competitive analysis:searched,screened,and assessed the existing DAs to gather insights for design strategies,developmental techniques,and functionalities;(ii)user needs assessment:interviewed family surrogates in our hospital to explore target user group's decision-making experience and identify their unmet needs;(iii)evidence syntheses:integrate latest clinical evidence and pertinent information to inform the content development of *** The competitive analysis included 16 relevant DAs,from which we derived valuable insights using existing *** decision needs were explored among a cohort of 15 family surrogates,revealing four thematic issues in decision-making,including stuck into dilemmas,sense of uncertainty,limited capacity,and delayed decision confirmation.A total of 27 articles were included for evidence *** decision making knowledge on disease and treatment,as delineated in the literature sourced from decision support system or clinical guidelines,were formatted as the foundational knowledge ***-one items of evidence were extracted and integrated into the content panels of benefits and risks of RRT,possible outcomes,and reasons to *** DA was drafted into a web-based phototype using the elements of *** platform could guide users in their preparation of decision-making through a sequential four-step process:identifying

关键词： decision aids renal replacement therapy intensive care units shared decision-making user-centered design surrogate

The lactylation index predicts the immune microenvironment and prognosis of pan-cancer patients

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

BIOCELL 2024年第8期48卷 1223-1239页

作者： XUEJIA ZHAI JIE LIU JINWEI XIAO TAO ZHANG JUN WANG JIANJUN LI SHICANG YU Department of Oncology Southwest HospitalThird Military Medical University(Army Medical University)Chongqing400038China Department of Stem Cell and Regenerative Medicine Institute of Pathology and Southwest Cancer CenterSouthwest HospitalThird Military Medical University(Army Medical University)Chongqing400038China Key Laboratory of Cancer Immunopathology Ministry of EducationChongqing400038China International Joint Research Center for Precision Biotherapy Ministry of Science and TechnologyChongqing400038China Jin-feng Laboratory Chongqing401329China

Background:Protein lactylation is a new way for the“metabolic waste”lactic acid to perform novel ***,our understanding of the contribution of protein lactylation to both tumor progression and therapeutic interventions remains *** construction of a scoring system for lactylation to predict the prognosis of pancancer patients and to evaluate the tumor immune microenvironment(TIME)would improve our understanding of the clinical significance of ***:Consensus clustering analysis of lactylation-related genes was used to cluster 177 pancreatic adenocarcinoma(PAAD)***,a scoring system was developed using the least absolute shrinkage and selection operator(LASSO)*** validation and external validation were both conducted to assess and confirm the predictive accuracy of the scoring ***,leucine rich repeat containing 1(LRRC1),a newly discovered lactylation-related gene,was analyzed in PAAD in ***:Utilizing the profiles of 332 lactylation-related genes,a total of 177 patients with PAAD were segregated into two distinct ***^(high) patients had a poorer prognosis than LacCluster^(low) *** the differential analysis between the LacCluster^(high) and LacCluster^(low) groups,we identified additional genes associated with *** genes were then integrated to construct the LacCluster-enhanced system,which enabled more accurate prognosis prediction for patients with ***,a lactylation index containing three genes(LacI-3)was constructed using LASSO *** was done to enhance the usability of the LacCluster-enhanced system in the *** to those in the LacI-3^(high) subgroup,patients in the LacI-3^(low) subgroup exhibited increased expression of immune checkpoint-related genes,more immune cell infiltration,lower tumor mutation burdens,and better prognoses,indicating a“hot tumor”***,knocking down the expression of LRRC1,

关键词： Pancreatic adenocarcinoma Pan-cancer Lactylation Prognosis Tumor immune microenvironment LRRC1

The role of lipid metabolism in osteoporosis:Clinical implication and cellular mechanism

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genes & Diseases 2024年第4期11卷 220-233页

作者： Jing Zhang Wenhui Hu Zhi Zou Yuheng Li Fei Kang Jianmei Li Shiwu Dong College of Bioengineering Chongqing UniversityChongqing 400044China Department of Biomedical Materials Science College of Biomedical EngineeringArmy Medical University(Third Military Medical University)Chongqing 400038China State Key Laboratory of Trauma and Chemical Poisoning Army Medical University(Third Military Medical University)Chongqing 400038China

In recent years,researchers have become focused on the relationship between lipids and bone metabolism ***,many diseases related to lipid metabolism dis-orders,such as nonalcoholic fatty liver disease,atherosclerosis,obesity,and menopause,are associated with osteoporotic *** has been clinically observed in humans that these lipid metabolism disorders promote changes in osteoporosis-related indicators bone mineral density and bone ***,similar osteoporotic phenotype changes were observed in high-fat and high-cholesterol-induced animal *** lipid metabolism(such as increased oxidized lipids and elevated plasma cholesterol)affects bone microenvironment ho-meostasis via cross-organ communication,promoting differentiation of mesenchymal stem cells to adipocytes,and inhibiting commitment towards ***,disturbances in lipid metabolism affect the bone metabolism balance by promoting the secretion of cyto-kines such as receptor activator of nuclear factor-kappa B ligand by osteoblasts and stimulating the differentiation of ***,this review addresses the possible link be-tween lipid metabolism disorders and osteoporosis and elucidates the potential modulatory mechanisms and signaling pathways by which lipid metabolism affects bone metabolism *** also summarize the possible approaches and prospects of intervening lipid meta-bolismforosteoporosistreatment.

关键词： Bone homeostasis Cross-organ communication Lipid metabolism Osteoclast Osteoporosis

Seminoma characterized by thickening of the pituitary stalk: A case report

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences 2024年第6期49卷 863-869页

作者： Peng B. Wang X. Deng Y. Lian Y. Zheng Y. Kuang J. Qian J. Liang J. Zhang Y. Department of Endocrinology First Affiliated Hospital of Army Medical University 400038 Chongqing China Department of Radiology First Affiliated Hospital of Army Medical University 400038 Chongqing China Department of Endocrinology 958th Hospital of the Chinese People's Liberation Army 400020 Chongqing China Department of Neurosurgery First Affiliated Hospital of Army Medical University 400038 Chongqing China

颅内精原细胞瘤是一种罕见的起源于生殖细胞系的恶性肿瘤，通常发生在脑部的松果体或垂体等部位。陆军军医大学第一附属医院内分泌科于2020年6月收治１例颅内精原细胞瘤伴脊柱转移的20岁男性患者，临床表现为头痛、头晕及视力下降，头... 详细信息

颅内精原细胞瘤是一种罕见的起源于生殖细胞系的恶性肿瘤，通常发生在脑部的松果体或垂体等部位。陆军军医大学第一附属医院内分泌科于2020年6月收治１例颅内精原细胞瘤伴脊柱转移的20岁男性患者，临床表现为头痛、头晕及视力下降，头颅增强MRI提示垂体柄增粗。在多学科综合会诊后接受经鼻蝶鞍区肿瘤切除术，病理诊断为精原细胞瘤，术后规律放射治疗。1年后患者的肿瘤复发并转移，再次行胸椎椎管内肿瘤切除术，术后继续化学治疗。患者的临床症状如头痛和视力障碍得到改善，但持续全垂体功能减退，需长期激素替代。颅内精原细胞瘤的早期诊断颇具挑战性，且易发生转移，对放射治疗和化学治疗高度敏感，早期诊断和多学科综合治疗有助于改善患者的生活质量和预后。.; Intracranial seminoma is a rare malignant tumor originating from the germ cells, usually occurring in the pineal gland or pituitary gland. In June 2020, the Department of Endocrinology at the First Affiliated Hospital of Army Military Medical University admitted a 20-year-old male patient with an intracranial germ cell tumor and spinal metastases. The patient presented with headache, dizziness, and visual impairment. Enhanced magnetic resonance imaging (MRI) of the head indicated thickening of the pituitary stalk. After multidisciplinary consultation, the patient underwent endonasal transsphenoidal resection of the tumor, with the pathological diagnosis confirming germ cell tumor. The patient received regular radiotherapy postoperatively. One year later, the tumor recurred and metastasized, leading to a second surgery for tumor resection in the thoracic spinal canal, followed by continued chemotherapy. The patient's clinical symptoms, such as headache and visual disturbances, improved, but he continued to experience panhypopituitarism and required long-term hormone replacement therapy. Early diagnosis of intracranial germ cell tumors is challenging, and they are prone to metastasis and highly sensitive to radiotherapy and chemotherapy. Early diagnosis and multidisciplinary comprehensive treatment can help improve the quality of life and prognosis for patients.

关键词： immunohistochemistry intracranial seminoma magnetic resonance imaging surgery

FOXO1 reshapes neutrophils to aggravate acute brain damage and promote late depression after traumatic brain injury

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Military Medical Research 2024年第4期11卷 521-542页

作者： Mi Zhou Yang-Wu-Yue Liu Yu-Hang He Jing-Yu Zhang Hao Guo Hao Wang Jia-Kui Ren Yi-Xun Su Teng Yang Jia-Bo Li Wen-Hui He Peng-Jiao Ma Man-Tian Mi Shuang-Shuang Dai Department of Biochemistry and Molecular Biology School of Basic MedicineArmy Medical UniversityChongqing 400038China Research Center for Nutrition and Food Safety Chongqing Key Laboratory of Nutrition and HealthInstitute of Military Preventive MedicineArmy Medical UniversityChongqing 400038China Department of Neurosurgery Daping HospitalArmy Medical UniversityChongqing 400042China Department of Trauma and Emergency Southwest HospitalArmy Medical UniversityChongqing 400038China Department of Histology and Embryology Chongqing Key Laboratory of NeurobiologyBrain and Intelligence Research Key Suyixun Laboratory of Chongqing Education CommissionArmy Medical UniversityChongqing 400038China Research Center Seventh Affiliated Hospital of Sun Yat-Sen UniversityShenzhen 518107GuangdongChina

Background:Neutrophils are traditionally viewed as first responders but have a short onset of action in response to traumatic brain injury(TBI).However,the heterogeneity,multifunctionality,and time-dependent modulation of brain damage and outcome mediated by neutrophils after TBI remain poorly ***:Using the combined single-cell transcriptomics,metabolomics,and proteomics analysis from TBI patients and the TBI mouse model,we investigate a novel neutrophil phenotype and its associated effects on TBI outcome by neurological deficit scoring and behavioral *** also characterized the underlying mechanisms both invitro and invivo through molecular simulations,signaling detections,gene expression regulation assessments[including dual-luciferase reporter and chromatin immunoprecipitation(ChIP)assays],primary cultures or co-cultures of neutrophils and oligodendrocytes,intracellular iron,and lipid hydroperoxide concentration measurements,as well as forkhead box protein O1(FOXO1)conditional knockout ***:We identified that high expression of the FOXO1 protein was induced in neutrophils after TBI both in TBI patients and the TBI mouse *** of these FOXO1high neutrophils in the brain was detected not only in the acute phase but also in the chronic phase post-TBI,aggravating acute brain inflammatory damage and promoting late TBI-induced *** the acute stage,FOXO1 upregulated cytoplasmic Versican(VCAN)to interact with the apoptosis regulator B-cell lymphoma-2(BCL-2)-associated X protein(BAX),suppressing the mitochondrial translocation of BAX,which mediated the antiapoptotic effect companied with enhancing interleukin-6(IL-6)production of FOXO1high *** the chronic stage,the“FOXO1-transferrin receptor(TFRC)”mechanism contributes to FOXO1high neutrophil ferroptosis,disturbing the iron homeostasis of oligodendrocytes and inducing a reduction in myelin basic protein,which contributes to the progression of late depression afte

关键词： Traumatic brain injury(TBI) Neutrophil Forkhead box protein O1(FOXO1) Acute stage Chronic stage

维普期刊数据库

生物节律因素对体外循环心脏手术后AKI发生的影响：回顾性队列研究

在线全文

学校读者我要写书评

暂无评论

中华麻醉学杂志 2024年第9期44卷 1093-1096页

作者：李小东文静徐小廖先建宋雨汐段家翔鲁开智易斌甯交琳 400038

目的评价生物节律因素对体外循环心脏手术后急性肾损伤(AKI)发生的影响。方法本研究为回顾性队列研究。收集2018年6月至2019年12月体外循环心脏手术患者的病例资料，按照实施麻醉手术的时间分为2组( n ＝125)：上午节律组(Ⅰ组)8：00... 详细信息

目的评价生物节律因素对体外循环心脏手术后急性肾损伤(AKI)发生的影响。方法本研究为回顾性队列研究。收集2018年6月至2019年12月体外循环心脏手术患者的病例资料，按照实施麻醉手术的时间分为2组( n ＝125)：上午节律组(Ⅰ组)8：00至12：00实施麻醉手术；下午节律组(Ⅱ组)14：00至18：00实施麻醉手术。记录患者术后AKI及术后其他相关并发症(肺部感染、脓毒症、脑梗死)发生情况。结果与Ⅱ组相比，Ⅰ组术后AKI发生率升高( P 0.05)。结论生物节律因素影响体外循环心脏手术后AKI发生，下午较上午实施手术有助于降低术后AKI发生。

关键词：生物节律体外循环急性肾损伤队列研究心脏手术