检索结果-南通市图书馆

MiR-139-5p inhibits migration and invasion of colorectal cancer by downregulating AMFR and NOTCH1

Protein & Cell 2014年第11期5卷 851-861页

作者： Mingxu Song Yuan Yin Jiwei Zhang Binbin Zhang Zehua Bian Chao Quan Leyuan Zhou Yaling Hu Qifeng Wang Shujuan Ni Bojian Fei Weili Wang Xiang Du Dong Hua Zhaohui Huang Wuxi Oncology Institute the Affiliated Hospital of Jiangnan University Wuxi 214062 China Department of Surgical Oncology the Affiliated Hospital of Jiangnan University Wuxi 214062 China Department of Radiation Oncology the Affiliated Hospital of Jiangnan University Wuxi 214062 China Department of Pathology Fudan University Shanghai Cancer Center Shanghai 200032 China

MicroRNAs （miRNAs） that exert function by posttran- scriptional suppression have recently brought insight in our understanding of the role of ***-coding RNAs in carcinogenesis and metastasis. In this study, we described the function and molecular mechanism of miR-139-5p in colorectal cancer （CRC） and its potential clinical application in CRC. We found that miR-139-5p was significantly downregulated in 73.8% CRC samples compared with adjacent noncancerous tissues （NCTs）, and decreased miR-139-5p was associated with poor prognosis. Functional analyses demonstrated that ectopic expression of miR-139-5p suppressed CRC cell migration and invasion in vitro and metastasis in vivo. Mechanistic investigations revealed that miR-139-5p suppress CRC cell invasion and metastasis by targeting AMFR and NOTCH1. Knockdown of the two genes phe- nocopied the inhibitory effect of miR-139-5p on CRC metastasis. Furthermore, the protein levels of the two genes were upregulated in CRC samples compared with NCTs, and inversely correlated with the miR-139-5p expression. Increased NOTCH1 protein expression was correlated with poor prognosis of CRC patients. Together, our data indicate that miR-139-5p is a potentialtumor suppressor and prognostic factor for CRC, and targeting miR-139-5p may repress the metastasis of CRC and improve survival.

关键词： miR-139-5p AMFR NOTCH1 colorectalcancer metastasis prognosis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Nucleolin promotes tumor growth in colorectal cancer by enhancing hnRNPA1-mediated PKM2 alternative splicing

引用

Genes & Diseases 2023年第6期10卷 2237-2240页

作者： Xue Wang Han Cheng Die Hu Ying Chen Waseem Hassan Jing Zhao Jiuming Li Zhaohui Huang Laboratory of Cancer Epigenetics Wuxi School of MedicineJiangnan UniversityWuxiJiangsu 214062China Wuxi Cancer Institute Affiliated Hospital of Jiangnan UniversityWuxiJiangsu 214062China Wuxi School of Medicine Jiangnan UniversityWuxiJiangsu 214062China Department of Pharmacy COMSATS University IslamabadLahore CampusLahore 54000Pakistan

Emerging evidence indicates that metabolism reprogramming plays an important role in cancer *** protein nucleolin(NCL)was reported to function as an important oncogenic factor in multiple cancer ***,the role and mechanism of NCL in cancer metabolism are *** this study,we found that NCL directly interacted with hnRNPA1 and promoted CRC cell proliferation by enhancing aerobic ***,NCL bound PKM pre-mRNA and increased hnRNPA1-mediated PKM alternative splicing,resulting in increased PKM2 expression and tumor growth.A seed-mediated growth approach was used to synthesize gold nanostars(GNS),which were further modified with aptamer AS1411(an NCL ligand),GE11(an EGFR ligand),and nuclear localization signal to obtain functionalized nanoparticles(GNSAS1411-GE11).GNS-AS1411-GE11 efficiently enter the nucleus of CRC cells and blocked the glycolysis-promoting effects of NCL,inhibiting the growth of CRC *** NCL is a promising strategy for treating CRC.

关键词： PKM2 metabolism mediated

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Clinical correlation of B7-H3 and B3GALT4 with the prognosis of colorectal cancer

引用

World Journal of Gastroenterology 2018年第31期24卷 3538-3546页

作者： Ting Zhang Fang Wang Jing-Yi Wu Zhi-Chao Qiu Yan Wang Fen Liu Xiao-Song Ge Xiao-Wei Qi Yong Mao Dong Hua Institute of Cancer Affiliated Hospital of Jiangnan UniversityWuxi 214062Jiangsu ProvinceChina Department of Oncology Affiliated Hospital of Jiangnan UniversityWuxi 214062Jiangsu ProvinceChina Wuxi School of Medicine Jiangnan UniversityWuxi 214122Jiangsu ProvinceChina Department of Pathology Affiliated Hospital of Jiangnan UniversityWuxi 214062Jiangsu ProvinceChina

AIM To investigate the expression and clinical significance of B7 homolog 3(B7-H3) and β-1,3-galactosyltransferase-4(B3 GALT4) in colorectal cancer(CRC) *** Using tissue microarray, we identified the expression of B7-H3 and B3 GALT4 in 223 CRC patient samples by immunohistochemistry and evaluated the possible correlation between B7-H3 and B3 GALT4 and clinicaloutcomes. Further, the m RNA and protein expression were identified to establish the regulatory relationship of B7-H3 with B3 GALT4 in *** A significant positive correlation between B7-H3 and B3 GALT4 was observed in CRC specimens(r = 0.219, P = 0.001). High expression of B7-H3 was identified as a significant independent predictor of poor overall survival(OS) [hazard ratio(HR) = 1.781; 95%CI: 1.027-3.089; P = 0.040]. Moreover, high expression of B3 GALT4 was also recognized as an independent predictor of inferior OS(HR = 1.597; 95%CI: 1.007-2.533; P = 0.047). Additionally, CRC patients expressing both high B7-H3 and high B3 GALT4 contributed to a significant decrease in OS(HR = 2.283; 95%CI: 1.289-4.042; P = 0.005). In CRC cell lines with stable expression of high B7-H3, the m RNA and protein expressions of B3 GALT4 were significantly upregulated. Similarly, the expression of B3 GALT4 was significantly reduced when expression of B7-H3 was knocked *** The expression of B3 GALT4 in CRC is positively correlated with B7-H3 expression in vitro. B7-H3/B3 GLAT4 may be used as dual prognostic biomarkers for CRC.

关键词： B7 homolog 3 β-1,3-galactosyltransferase-4 Colorectal cancer Prognosis Correlation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Correction to:MiR-139-5p inhibits migration and invasion of colorectal cancer by downregulating AMFR and NOTCH1

引用

Protein & Cell 2021年第8期12卷 667-669页

作者： Mingxu Song Yuan Yin Jiwei Zhang Binbin Zhang Zehua Bian Chao Quan Leyuan Zhou Yaiing Hu Qifeng Wang Shujuan Ni Bojian Fei Weiii Wang Xiang Du Dong Hua Zhaohui Huang Wuxi Oncology Institute the Affiliated Hospital of Jiangnan UniversityWuxi 214062China Department of Surgical Oncology the Affiliated Hospital of Jiangnan UniversityWuxi 214062China Department of Radiation Oncology the Affiliated Hospital of Jiangnan UniversityWuxi 214062China Department of Pathology Fudan University Shanghai Cancer CenterShanghai 200032China

CORRECTION TO:PROTEIN CELL(2014)5(11):851-861 HTTPS://***/10.1007/S13238-014-0093-5 In the original publication the display of Fig.1 is in *** correct Fig.1 is available in this correction.

关键词： invasion colorectal cancer

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

MicroRNA-638 inhibits cell proliferation by targeting phospholipase D1 in human gastric carcinoma

引用

Protein & Cell 2015年第9期6卷 680-688页

作者： Jiwei Zhang Zehua Bian Jialiang Zhou Mingxu Song Zhihui Liu Yuyang Feng Li Zhe Binbin Zhang Yuan Yin Zhaohui Huang Wuxi Oncology Institute The Affiliated Hospital of Jiangnan University Wuxi 214062 China Department of Radiation Oncology The Affiliated Hospital of Jiangnan University Wuxi 214062 China Fudan University Shanghai Cancer Center and Institutes of Biomedical Sciences Shanghai Medical CollegeFudan University Shanghai 200032 China

MicroRNAs （miRNAs） are a type of small non-coding RNAs that are often play important roles in carcinogene- sis, but the carcinogenic mechanism of miRNAs is still unclear. This study will investigate the function and the mechanism of miR-638 in carcinoma （GC）. The expres- sion of miR-638 in GC and the DNA copy number of miR- 638 were detected by real-time PCR. The effect of miR-638 on cell proliferation was measured by counting kit-8 assay. Different assays, including bioinformatics algo- rithms （TargetScan and miRanda）, luciferase report assay and Western blotting, were used to identify the target gene of miR-638 in GC. The expression of miR-638 target gene in clinical CRC tissues was also validated by immunohistochemical assay. From this research, we found that miR-638 was downregulated in GC tissues compared with corresponding noncancerous tissues （NCTs）, and the DNA copy number of miR-638 was lower in GC than NCTs, which may induce the corresponding downregulation of miR-638 in GC. Ectopic expression of miR-638 inhibited GC cell growth in vitro. Subsequently, we identified that PLD1 is the target gene of miR-638 in GC, and silencing PLD1 expression phenocopied the inhibitory effect of miR-638 on GC cell proliferation. Fur- thermore, we observed that PLD1 was overexpressed inGC tissues, and high expression of PLDt in GC predicted poor overall survival. In summary, we revealed that miR- 638 functions as a tumor suppressor in GC through inhibiting PLDI.

关键词： gastric cancer miR-638 cell proliferation,PLD1

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

microRNA-18a Promotes Cell Migration and Invasion Through Inhibiting Dicer l Expression in Hepatocellular Carcinoma In Vitro

引用

Chinese Medical Sciences Journal 2017年第1期32卷 34-43页

作者： Xiufen Zhang Bo Yu Fuzheng Zhang Zijian Guo Lihua Li Institute of Oncology The Affiliated Hospital of Jiangnan University Wuxi Jiangsu 214062 China Department of Oncology People’s Hospital of TaixingTaixing Jiangsu 225400 China Department of Oncology The Affiliated Hospital of Jiangnan University Wuxi Jiangsu 214062 China Department of Surgerical Oncology The Affiliated Hospital of Jiangnan University Wuxi Jiangsu 214062 China

Objective To investigate the effects of microRNA-18a(miR-18a) on migration and invasion of hepatocellular carcinoma(HCC) cells,and its possible mechanism associated with Dicer l. Methods HepG 2 and HepG 2.2.15 cells were transfected with miR-18 a inhibitor using Lipofectamine. Cell invasion was evaluated by transwell invasion assay,and cell migration was detected by transwell migration and wound-healing assays. Moreover,luciferase reporter assay was used to identify whether Dicer expression was regulated by miR-18 a. Real-time RT-PCR and western blot were performed to analyze Dicer 1 expression. In addition,a functional restoration assay was performed to investigate whether miR-18 a promotes HCC cell migration and invasion by directly targeting Dicer 1. Results miR-18 a inhibitor can suppress the migration and invasion of HCC cells. Furthermore,suppression of Dicer l expression by small interfering RNA essentially abolished the inhibition of cell migration and invasion induced by miR-18 a inhibitor,restorating these activities to levels similar to the parental HCC cells. Interestingly,suppression of miR-18 a in HCC cells resulted in enhanced expression of Dicer l. In addition,the results of a luciferase assay demonstrated targeted regulation of Dicer l by miR-18 a. Conclusion Our findings suggest that miR-18 a promotes migration and invasion of HCC cells by inhibiting Dicer l expression.

关键词： hepatocellular carcinoma microRNA-18a, Dicer l migration invasion

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Prevalence and risk factors on age-related cataract and surgery in adults over 50 years old in Binhu District, Wuxi, China

引用

International Journal of Ophthalmology(English edition) 2020年第3期13卷 445-451页

作者： Xing Chen Dan-Ying Zhou Jian Shen Yan-Bing Wu Qing-Zhu Sun Jian-Min Dong Jian-Chun Yu Department of Ophthalmology Wuxi 9th Affiliated Hospital of Soochow UniversityWuxi 214062Jiangsu ProvinceChina

AIM:To investigate the prevalence and risk factors of age-related cataract(ARC),ARC surgery procedures,and postoperative vision results among adults over 50 years old in the Binhu District of Wuxi City,***:Thirty basic sampling units were analyzed via a cluster random sampling *** medical histories were collected and eye examinations were *** prevalence and surgical procedures were ***:Among the 6150 participants,1421 cataract cases were diagnosed and prevalence was 23.1%.The prevalence of cortical,nuclear,and posterior subcapsular cataracts increased with age(P0.05).The odds ratio of cataract patients who had or did not have cataract surgery was 3.15(87/28)and the frequency of cataract blindness surgery was 75.7%(87/115).Poor visual outcomes was in 107 eyes(40.7%)after cataract *** vision was mostly caused by uncorrected reflective errors(30.9%)and ocular comorbidities(41.1%).The prevalence of cataract surgery complications was 5.7%(15/263).Surgical complications and posterior capsular opacification were avoidable factors facilitating poor ***:ARC,especially in females and illiterateindividuals,presents a public health problem in this *** visual outcomes after cataract surgery are ***-quality cataract surgeries and treatment of ocular comorbidities are vital.

关键词： age-related cataract cataract surgery prevalence blindness

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

同指尺侧岛状皮瓣远侧V-Y推进修复拇指指端缺损

引用

中华整形外科杂志 2010年第6期26卷 414-416页

作者：周晓芮永军许亚军寿奎水姚群无锡市手外科医院 214062

目的探讨一种修复拇指指端缺损的手术方法. 方法对拇指指端缺损的患者采用同指尺侧岛状皮瓣远侧V-Y推进进行修复,术中切取皮瓣范围1.4 cm×2.0 cm～1.4 cm×2.5 cm,供区取前臂全厚皮片移植修复. 结果 2007年3月至2009年10月于临床应... 详细信息

目的探讨一种修复拇指指端缺损的手术方法. 方法对拇指指端缺损的患者采用同指尺侧岛状皮瓣远侧V-Y推进进行修复,术中切取皮瓣范围1.4 cm×2.0 cm～1.4 cm×2.5 cm,供区取前臂全厚皮片移植修复. 结果 2007年3月至2009年10月于临床应用10例,术后所有皮瓣和移植皮片均成活,伤口一期愈合.术后随访6～18个月,皮瓣质地柔软,无明显挛缩,末节指腹指纹重新建立,指间关节活动正常,无钩甲畸形发生,末节指腹两点辨别觉为5～6 mm. 结论应用同指尺侧岛状皮瓣远侧V-Y推进的方法可以较好地修复拇指指端缺损.

关键词：外科皮瓣拇指缺损

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

以膝上外侧穿支为蒂的V—Y皮瓣修复股前外侧皮瓣供区

引用

中华整形外科杂志 2014年第1期30卷 26-29页

作者：周晓薛明宇芮永军许亚军寿奎水卜凡玉无锡市手外科医院214062

【摘要】目的探讨应用膝上外侧穿支为蒂的V．Y皮瓣修复股前外侧皮瓣供区的疗效及适应证。方法2011年6月至2012年6月，对9例患者应用股前外侧皮瓣修复手、足创面的同时，采用以膝上外侧穿支为蒂的V—Y皮瓣修复股前外侧皮瓣供区，皮瓣与... 详细信息

【摘要】目的探讨应用膝上外侧穿支为蒂的V．Y皮瓣修复股前外侧皮瓣供区的疗效及适应证。方法2011年6月至2012年6月，对9例患者应用股前外侧皮瓣修复手、足创面的同时，采用以膝上外侧穿支为蒂的V—Y皮瓣修复股前外侧皮瓣供区，皮瓣与股前外侧皮瓣遗留创面等宽，而V—Y皮瓣供区直接拉拢缝合。结果术后9例股前外侧皮瓣全部成活，5例于术后6—8个月行皮瓣削薄术。术后随访5～18个月，平均8个月，皮瓣外观、质地、色泽及弹性良好。膝上外侧穿支皮瓣8例完全成活，1例皮瓣近端尖角发生部分坏死，经换药后愈合，伤口愈合时间平均为17d。大腿伤口愈合较平整，无明显凹陷，大腿股四头肌肌力正常，膝关节活动正常。结论该术式操作简单，术后疗效满意，是对传统股前外侧皮瓣供区修复方法的改进。

关键词：【关键词】外科皮瓣缺损供区

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

大鱼际部穿支皮瓣在拇指及虎口狭长瘢痕挛缩中的应用

引用

中华整形外科杂志 2013年第3期29卷 181-183页

作者：周晓许亚军芮永军寿奎水包岳丰无锡市手外科医院 214062

目的探讨大鱼际部穿支皮瓣在拇指及虎口局部狭长瘢痕挛缩中的应用及疗效。方法对2010年8月至2012年1月收治的9例拇指近节及虎口狭长瘢痕挛缩患者，行挛缩瘢痕切除松解后形成的8mm×20mm-15mm×30mm皮肤缺损，于近创面大鱼际两侧设计10mm... 详细信息

目的探讨大鱼际部穿支皮瓣在拇指及虎口局部狭长瘢痕挛缩中的应用及疗效。方法对2010年8月至2012年1月收治的9例拇指近节及虎口狭长瘢痕挛缩患者，行挛缩瘢痕切除松解后形成的8mm×20mm-15mm×30mm皮肤缺损，于近创面大鱼际两侧设计10mm×25mm～15mm×35mm的穿支皮瓣转移修复，供区直接拉拢缝合。结果术后皮瓣完全成活，创面、供区切口均一期愈合。患者均获随访6—18个月，皮瓣外形良好，质地柔软。拇指背伸及外展接近最大外展及背伸，虎口外展70°一90°，大鱼际两侧切口无瘢痕挛缩，供区无并发症。结论大鱼际部穿支皮瓣具有不损伤主干动脉的优点，是修复拇指及虎口局部狭长瘢痕挛缩的理想方法之一。

关键词：穿支皮瓣瘢痕挛缩

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：