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Expression and localization of Wolfram syndrome 1 gene in the developing rat pancreas

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World Journal of Gastroenterology 2009年第43期15卷 5425-5431页

作者： Rong Xu Biao Xia Jie Geng Jing Shi Hui Shi Li Yuan Wei De Department of Biochemistry and Molecular Biology Nanjing Medical University Nanjing 210029 Jiangsu Province China First School-of Clinical Medicine Nanjing Medical University Nanjing 210029 Jiangsu Province China

AIM： To investigate the expression and function of Wolfram syndrome 1 gene （WFS1） during the development of normal pancreas. METHODS： Pancreas from Sprague-Dawley rat fetuses, embryos, young and adult animals were used in this study. Expression levels of WFS1 in pancreas of different development stages were detected by reverse transcription-polymerase chain reation （RT-PCR） and Western blotting. To identify the cell location of WFS1 in the developing rat pancreas, double-immunofluorescent staining was performed using antibodies to specific cell markers and WFS1, respectively. RESULTS： Compared to E15.5, the highest level of WFSl mRNA was detected at E18.5, the level of WFSl mRNA in E15.5 and P0 was less, and at a lowest at adult （P 〈 0.05 vs P0 and adult）, respectively. Compare to E15.5, the highest level of WFS1 was at P14 and lowest at P21 （P 〈 0.05 vs P14 and P21）, respectively. The WFSl positive staining is expressed in the normal developing rat pancreas mainly in the islet beta-cells and mesenchyme at each stage tested. CONCLUSION： These results indicate that WFSl may be involved in some aspects of pancreatic development and further research on WFS1 may provide new evidences to prove the interactions between mesenchyma and epithelia at the same time.

关键词： Wolfram syndrome 1 Pancreas Morphogenesis Mesenchyme Pancreatic beta cells

Antibiotics along an alpine river and in the receiving lake with a catchment dominated by grazing husbandry

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Journal of Environmental Sciences 2022年第5期34卷 374-382页

作者： Jianwei Dong Dandan Yan Kangle Mo Qiuwen Chen Jianyun Zhang Yuchen Chen Zhiyuan Wang State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering Nanjing Hydraulic Research InstituteNanjing 210029China Center for Eco-Environmental Research Nanjing Hydraulic Research InstituteNanjing 210029China Yangtze Institute for Conservation and Green Development Nanjing 210029China School of Marine Science and Engineering Nanjing Normal UniversityNanjing 210023China

The livestock breeding industries face overuse of antibiotics,which has been intensively studied in recent ***,the occurrence and fate of antibiotics as well as their potential threats to the aquatic environments in alpine and arid regions remain *** study investigated the relationship of the occurrence and concentrations of antibi-otics between the Kaidu River and Bosten Lake in a typical alpine basin in *** spots with antibiotic pollution source were *** antibiotic concentrations in river water and suspended sediment(SPS)were 2.20-99.4 ng/L and 1.03-176 ng/*** dominant antibi-otics were tetracyclines,sulphacetamide,and ofloxacin in river water and sulfonamides,clarithromycin,roxithromycin,and ofloxacin in *** apparent differences in pollution sources and landscapes in different reaches led to the obvious spatial patterns of antibiotics in the Kaidu *** partition coefficient of antibiotic between SPS and water phases for sulfonamides than tetracyclines was because that tetracyclines strongly responded to clay contents while sulfonamides significantly responded to organic carbon contents in *** were significant differences in detected antibiotic categories between the river and the ***(especially ciprofloxacin and enrofloxacin)were detected in the lake while sulphacetamide was only detected in the ***,the surrounding husbandry and aquaculture around the Bosten Lake was an important antibiotic pollution source in addition to inputs from the Kaidu *** research suggested that alpine lakes could be an important sink of antibiotics in alpine dry regions,and thus impose greater threats to the aquatic ecosystem.

关键词： Antibiotics Lotic-lentic contamination Suspended sediment Partition

Bortezomib depended on PRDM1 and TP53 to exert therapeutic effect in activated B-cell-like diffuse large B-cell lymphoma

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Genes & Diseases 2024年第2期11卷 550-553页

作者： Jing Tang Yue Li Jiazhu Wu Haorui Shen Hua Yin Jinhua Liang Li Wang Jianyong Li Yi Xia Wei Xu Department of Hematology The First Affiliated Hospital of Nanjing Medical UniversityJiangsu Province HospitalNanjingJiangsu 210029China Key Laboratory of Hematology of Nanjing Medical University NanjingJiangsu 210029China Collaborative Innovation Center for Cancer Personalized Medicine NanjingJiangsu 210029China

PR/SET domain 1(PRDM1)gene is located on chromosome 6q21,encoding the B lymphocyte-induced maturation protein 1(BLIMP1).1 It is reported that loss of PRDM1 function is exacerbated in activated B-cell-like(ABC)-diffuse large B cell lymphoma(DLBCL)and associated with inferior ***,it remains unclear what leads to PRDM1 inactivation and the drug resistance mechanism caused by abnormal inactivation of *** investigated the contribution of PRDM1 gene as a prognosis and potential therapeutic target for ABC-DLBCL patients and further clarified the possible mechanism of PRDM1 abnormal *** first proposed that TP53 could regulate PRDM1 by histone ubiquitination modification at the post-transcriptional level.

关键词： PRDM1 TP53 therapeutic

Expression and location of α-fetoprotein during rat colon development

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World Journal of Gastroenterology 2009年第14期15卷 1738-1743页

作者： Xiao-Yan Liu Dan Dong Peng Sun Jun Du Luo Gu Ying-Bin Ge Department of Physiology Nanjing Medical University Nanjing 210029 Jiangsu Province China

AIM： To investigate the expression of α-fetoprotein （AFP）, a cancer-associated fetal glycoprotein, and its involvement during rat colon development. METHODS： Colons from Sprague-Dawley rat fetuses, young and adult （8 wk old） animals were used in this study. Expression levels of AFP in colons of different development stage were detected by reversetranscriptase PCR （RT-PCR） and Western blotting. To identify the cell location of AFP in the developing rat colons, double-immunofluorescent staining was performed using antibodies to specific cell markers and AFP, respectively. RESULTS： The highest levels of AFP mRNA were detected in colons of rats at embryonic day 18.5 （e18.5）. Compared to e18.5 d, the AFP expression was significantly decreased during rat development （85% for e20.5, P 〈 0.05, 58% for postnatal day 0.5 （P〈0.5）, P 〈 0.05, 37% for P7, P 〈 0.05, 24% for P14, P 〈 0.05, and 11% for P21, P 〈 0.05） and undetected in adult rats. Only the 72-kDa isoform of AFP was detected by Western blotting, the expression pattern was similar to AFP mRNA and conformed to the results of mRNA expression. The AFP positive staining was identical to different distribution patterns in fetuses, young and adult animals and positive staining for both AFP and vimentin was overlapped in mesenchymal cells at each stage tested. CONCLUSION： This study has for the first time demonstrated that AFP is localized in the mesenchyme of rat colon from the embryo to the weaning stage by immunofluorescence and presents 72-kDa isoform in the developing rat colons by Western blotting. The dynamic expression of AFP in the various developmental stages of the colon indicates that AFP might be involved in many aspects of colon development.

关键词： Alpha-fetoprotein Development Mesenchyme Colon Rat

Study on Wave Dissipation of the Structure Combined by Baffle and Submerged Breakwater

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China Ocean Engineering 2017年第6期31卷 674-682页

作者： JU Lie-hong zuo Qi-hua TENG Ling WANG Xing-gang Nanjing Hydraulic Research Institute Nanjing 210029 China College of Harbor Coastal and Offshore Engineering Hohai University Nanjing 210098 China Key Laboratory of Port Waterway and Sedimentation Engineering of the Ministry of Transport Nanjing 210029 China

This paper proposes a structure combined by baffle and submerged breakwater （abbreviated to SCBSB in the following texts）. Such a combined structure is conducive to the water exchange in the harbor, and has strong capability on wave dissipation. Our paper focuses on the discussion of two typical structures, i.e., the submerged baffle and rectangular breakwater combined with the upper baffle respectively, which are named as SCBSB 1 and SCBSB2 for short. The eigenfunction method corrected by experimental results is used to investigate the wave dissipation characteristics. It shows that the calculated results agree well with the experimental data and the minimum value of the wave transmission coefficient can be obtained when the distance between the front and rear structures is from 1/4 to 1/2 of the incident wave length.

关键词： combined wave dissipation structure eigenfunction method physical model experiment transmissioncoefficient correction wave dissipation effect

超大城市洪涝灾害成因综合解析——以重庆市为例

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土木与环境工程学报（中英文） 2025年第1期47卷 1-10页

作者：仉文岗王云浩胡庆芳孙伟鑫金君良重庆大学土木工程学院重庆400045 南京水利科学研究院水文水资源与水利工程科学国家重点实验室南京210029

近年来,极端天气事件呈现趋多趋强态势,变化环境下洪涝灾害频发给社会经济发展带来严峻挑战。重庆是中国西部超大城市和成渝城市群“双核”之一,主城区地貌以山地、丘陵为主,受上游江河来水和三峡库区回水的双重影响,洪涝灾害风险突出... 详细信息

近年来,极端天气事件呈现趋多趋强态势,变化环境下洪涝灾害频发给社会经济发展带来严峻挑战。重庆是中国西部超大城市和成渝城市群“双核”之一,主城区地貌以山地、丘陵为主,受上游江河来水和三峡库区回水的双重影响,洪涝灾害风险突出。以重庆市历年洪涝灾害事件为基础,梳理重庆市防涝减灾现状,分析水文、地理、地形、城市化、气象等因素对重庆市洪涝灾害发生频率和强度的影响,结合这些洪涝灾害致灾因子进一步对重庆市的孕灾环境和承灾体进行分析,提出针对重庆中心城区洪涝治理的对策建议。分析发现:重庆市洪涝灾害受多种因素综合影响,特殊的地理位置和山地城市的地形条件决定了重庆防洪治涝的艰巨性;重庆市河流纵横,山高谷深,完全具备发生洪涝灾害的孕灾条件;应充分考虑气象水文和经济社会发展的新情况,根据三峡水库等建成后的洪涝灾害特征和城区防洪防涝能力现状,及时优化城市洪涝灾害防御体系。

关键词：城市灾害洪涝灾害防灾减灾防洪规划

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Fertilization of in vitro matured human oocytes by intracytoplasmic sperm injection (ICSI) using ejaculated and testicular spermatozoa

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Asian Journal of Andrology 2005年第1期7卷 39-43页

作者： TingFengt JuanChen Ling-BoCai Jia-YinLiu Yun-DongMao WeiDing HumanClinicalReproductiveMedicineCenter DepartmentofObstetrics＆GynecologyTheFirstAffiliatedHospitalofNanjingMedicalUniversityNanjing210029China

Aim: To evaluate the fertilization competence of spermatozoa from ejaculates and testicle when the oocytes were matured in vitro following intracytoplasmic sperm injection (ICSI). Methods: Fifty-six completed cycles in 46 women with polycystic ovarian syndrome were grouped according to the semen parameters of their male partners. Group 1 was 47 cycles that presented motile and normal morphology spermatozoa in ejaculates and Group 2 was the other nine cycles where male partners were diagnosed as obstructive azoospermia and spermatozoa could only be found in testicular tissue fragment. All female patients received minimal stimulation with gonadotropin. Immature oocytes were matured in vitro and inseminated by ICSI. The spermatozoa from testes were retrieved by testicular fine needle aspiration. Results: A total of 449 and 78 immature oocytes were collected and cultured for 48 hours, 75.5 % (339/449) and 84.6 % (66/78) oocytes were matured in Groups 1 and 2, respectively. The percentage of oocytes achieving normal fertilization was significantly higher in Group 1 than that in Group 2 (72.9 % vs. 54.5 %, P < 0.05). There were no significant differences in the rates of oocytes cleavage and clinical pregnancies in these two groups [87.4 % (216/247) vs. 88.9 % (32/36); 21.3 % (10/47) vs. 44.4 % (4/9)]. A total of 15 babies in the two groups were healthy delivered at term. Conclusion: It appears that IVM combined with ICSI using testicular spermatozoa can produce healthy infants, while the normal fertilization rate of in vitro matured oocytes after ICSI using testicular spermatozoa was significantly lower than using the ejaculated spermatozoa.

关键词： intracytoplasmic sperm injection immature human oocytes in vitro maturation testicular fine needle sperm aspirations

Timing of brain computed tomography for predicting neurological prognosis in comatose cardiac arrest survivors: a retrospective observational study

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World Journal of Emergency Medicine 2022年第5期13卷 349-354页

作者： Gan-nan Wang Zhong-man Zhang Wen Chen Xiao-quan Xu Jin-song Zhang Department of Emergency the First Affi liated Hospital of Nanjing Medical UniversityNanjing 210029China Department of Radiology the First Affi liated Hospital of Nanjing Medical UniversityNanjing 210029China

BACKGROUND:To assess the association between relevant brain computed tomography(CT)parameters at different time and neurological prognosis in adult comatose survivors after cardiac arrest(CA).METHODS:A total of 94 CA patients who underwent early and late CT scans(within 24 h and 24 h to 7 d respectively after CA)between January 2018 and April 2020 were enrolled in this retrospective *** to the Cerebral Performance Category(CPC)score at hospital discharge,the patients were divided into either a good outcome(CPC 1-2)group or a poor-outcome group(CPC 3-5).The grey-to-white matter ratio(GWR)and the proportion of cerebrospinal fluid volume(pCSFV)were *** predicting poor outcomes,the prognostic performance of relevant CT parameters was evaluated,and the comparison analysis(expressed as the ratio of parameters in late CT to those in the early CT)of diff erent CT time was ***:Totally 26 patients were in the good-outcome group,while 68 patients were in the poor-outcome *** putamen density,GWR,and pCSFV in late CT were significantly lower in the poor-outcome group(P24 h after CA may be a good choice as a neuroimaging approach to evaluating *** predict neurological prognosis,comparison analysis of diff erent CT time can be used as another promising tool in comatose CA survivors.

关键词： Cardiac arrest Brain computed tomography Cerebral edema Coma Prognosis

Serum microRNA-155 as a potential biomarker for breast cancer screening

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Chinese Science Bulletin 2012年第26期57卷 3466-3468页

作者： ZHAO SuYing WU Qian GAO Fen ZHANG ChunBing YANG XueWen Department of Laboratory Medicine Jiangsu Provincial Hospital of TCMNanjing 210029China School of Public Health Nanjing Medical UniversityNanjing 210029China

MicroRNAs(miRs) have been shown to be differentially expressed in the serum of cancer patients and controls,and can thus be used as biomarkers for cancer *** detected the expression level of miR-155 in the serum of female breast cancer patients and healthy controls to investigate whether serum miR-155 could discriminate patients with early-stage breast *** samples were collected from 20 female patients with newly diagnosed breast cancer and 10 healthy ***-time quantitative PCR was used to detect the expression level of *** expression level of miR-155 was significantly increased in the serum of breast cancer patients compared with in the serum of normal ***-155 may be useful as a blood-based biomarker for breast cancer screening.

关键词：血清样品生物标志物乳腺癌筛查癌症患者小分子RNA PCR方法对照组