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体医融合促进体育产业可持续发展的策略研究

文体用品与科技 2025年第1期 61-63页

作者：贾月新江苏卫生健康职业学院江苏南京210029

体医融合是在健康中国建设背景下促进全民健康、助力体育消费升级的一个路径。体医融合高质量发展对于促进体育产业可持续发展具有重要促进意义。因此,本研究采用文献资料法、专家访谈法以及实地调研法等方法,对体医融合促进体育产业可... 详细信息

体医融合是在健康中国建设背景下促进全民健康、助力体育消费升级的一个路径。体医融合高质量发展对于促进体育产业可持续发展具有重要促进意义。因此,本研究采用文献资料法、专家访谈法以及实地调研法等方法,对体医融合促进体育产业可持续发展进行研究,重点包括存在问题及实施策略。问题:政策导向不足造成体医相关产业发展滞后、体医融合专业人才匮乏、体医融合基础设施保障不健全以及体医融合数字化建设不足。策略:构建系统完善的全民健身服务体系,培育新型体育消费增长点;出台地方性体医融合政策,提高政策制定合理性;大力发展体育用品制造业,促进体育消费;完善权责分明管理制度,构建协调运行机制。

关键词：体医融合健康中国全民健身体育产业发展

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Expression of a novel bHLH-Zip gene in human testis

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Asian Journal of Andrology 2003年第2期5卷 83-88页

作者： Jia-HaoSHA Zuo-MinZHOU Jian-MinLI MingLIN: HuiZHU HuZHU Ya-DongZHOU Li-LongWANG Yi-QuanWAIG Kai-YaZHOU Keylaboratoryofreproductivemedicine NanjingmedicalUniversityNanjing210029China Instituteofgeneticresources NanjingnormalUniversityNanjing210029China

Aim: To identify specifically expressed genes in the adult and fetal testes. Methods: A human testis cDNA microarray was established. Then the mRNA of adult and fetal testis was purified and probes were prepared by a reverse transcription reaction with the testis mRNA as template. The microarray was hybridized with probes of adult and fetal testes. The nucleic sequences of differentially expressed genes were determined and homologies were searched in the databases of the GenBank. Results: When hybridized with adult or fetal testis probes, the positive clones were 96.8 % and 95.4 %, respectively. Among these genes, one was a new testis-specific gene, which was named TSP1. TSP1 was highly expressed in human adult testis. The cDNA of TSP1 was 1,484 bp in length. The cDNA sequence of this clone was deposited in the Genbank (AF333098). TSP1 was also determined as Interim Gen Symbol (Unigene, No. Hs.98266). Protein analysis showed that TSP1 contained two functional domains: an N-terminal basic helix-loop-helix (bHLH) and a C-terminal leucine zipper (Zip). Homologous analysis showed that the 430 amino acid sequences deduced from the 1,293 bp open reading frame (ORF) had a homology with the human gene FLJ2509 (AK098575). TSP1 had also a sequence homology with Spz 1 protein of mouse. Expression profiles showed that TSP1 was specifically and strongly expressed in the testis. Conclusion: TSP1 is a gene highly expressed in adult testis. It may play an important role in spermatogenesis in the humans.

关键词： basic helix-loop-helix leucine zipper testis spermatogenesis DNA microarrays

Incipient Motion of Sediment Under Currents

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China Ocean Engineering 2000年第4期15卷 391-406页

作者：窦国仁 Chinese Academy of Sciences Nanjing 210029 China Chinese Society of Ocean Engineering Nanjing 210029 China Nanjing Hydraulic Research Institute Nanjing 210029 China

Summarized in the paper are the author's studies on incipient motion of sediment in recent 40 years. In addition to the forces of gravity, drag and lift, the cohesive force and the additional static pressure are important for fine particles. The relations between three stages of incipient motion are defined by use of instantaneous velocity. Formulas for initial velocity and critical shear stress are given and overall verified by the author's and others' experimental data.

关键词： sediment incipient motion initial velocity critical shear stress

Expression of liver insulin-like growth factor 1 gene and its serum level in patients with diabetes

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World Journal of Gastroenterology 2004年第2期10卷 255-259页

作者： Jian-BoLi Cheng-YaWang Jia-WeiChen Zhen-QingFeng Hong-TaiMa DepartmentofEndocrinology FirstAffiliatedHospitalofNanjingMedicalUniversityNanjing210029JiangsuProvinceChina MolecularLaboratory FirstAffiliatedHospitalofNanjingMedicalUniversityNanjing210029JiangsuProvinceChina DepartmentofPathology NanjingMedicalUniversityNanjing210029JiangsuProvinceChina

AIM:To explore the effect of diabetic duration and blood glucose level on insulin like growth factor 1(IGF-1) gene expression and serum IGF-1 ***: Diabetes was induced into Sprague Dawley rats by alloxan and then the rats were subdivided into different groups with varying blood glucose level and diabetic *** parameters were measured as follows:IGF-1 mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR),IGF-1 peptide and serum IGF-1 concentration by enzymelinked immunosorbent assay (ELISA).RESULTS:During early diabetic stage (week 2),in comparison with normal control group (NC), IGF-1 mRNA(1.17±0.069 vs 0.79±0.048, P<0.001;1.17±0.069 vs 0.53±0.023, P<0.0005, respectively), IGF-1 peptide contents[(196.66±14.9)ng·mg^-1 vs(128.2±11.25)ng·mg^-1,P<0.0005;(196.66±14.9)ng·mg^-1 vs(74.43±5.33)ng·mg^-1, P<0.0001,respectively] were reduced in liver tissues of diabetic *** IGF-1 gene downregulation varied with glucose control level of the diabetic state,and deteriorated gradually further with duration of *** month 6, hepatic tissue IGF-1mRNA was 0.71±0.024 vs 1.12±0.056,P<0.001;0.47±0.021 vs 1.12±0.056, P<0.0005,***-1 peptide was (114.35±8.09) ng·mg^-1 vs (202.05±15.73)ng·mg^-1,P<0.0005; (64.58±3.89) ng·mg^-1 vs(202.05±15.73)ng·mg^-1,P<0.0001 respectively. Serum IGF-1 was also lowered in diabetic group with poor control of blood *** week 2, serum IGF-1 concentrations were (371.0±12.5)ng·mg^-1 vs(511.2±24.7)ng·mg^-1,P<0.0005, (223.2±9.39)ng·mg^-1 vs(511.2±24.7)ng·mg^-1, P<0.0001 respectively. By month 6, (349.6±18.62)ng·mg^-1 vs(520.7±26.32)ng·mg^-1,P<0.0005, (188.5±17.35 vs520.7±26.32)ng·mg^-1,P<0.0001,respectively. Serum IGF-1 peptide change was significantly correlated with that in liver tissue (r=-0.99, P<0.001).Furthermore,No difference was found in the above parameters between diabetic rats with euglycemia and non-diabetic control ***: The influence of diabetic status on IGF-1 gene expression in li

关键词：胰岛素样生长因子1 基因表达血清学检查糖尿病逆转录聚合酶链反应 ELISA 动物模型

CHARACTERISTICS OF FLOW RESISTANCE IN OPEN CHANNELS WITH NON-SUBMERGED RIGID VEGETATION

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Journal of Hydrodynamics 2008年第2期20卷 239-245页

作者： WU Fu-sheng Nanjing Hydraulic Research Institute Nanjing 210029 China State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering Nanjing 210029 China

The flow resistance factors of non-submerged rigid vegetation in open channels were analyzed. The formulas of drag coefficient CD and equivalent Manning＇s roughness coefficient na were derived by analyzing the force of the flow of non-submerged rigid vegetation in open channel. The flow characteristics and mechanism of non-submerged rigid vegetation in open channel were studied through flume experiments.

关键词： characteristics of flow resistance non-submerged rigid vegetation drag coefficient equivalent Manning's roughness coefficient

Correlation of tumor-positive ratio and number of perigastric lymph nodes with prognosis of patients with surgically-removed gastric carcinoma

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World Journal of Gastroenterology 2004年第2期10卷 182-185页

作者： Yong-BinDing Guo-YuChen Jian-GuoXia Xi-WeiZang Hong-YuYang LiYang Xue-XianLiu DepartmentofGeneralSurgery FirstAffiliatedHospitalofNanjingMedicalUniversityNanjing210029JiangsuProvinceChina NanjingUniversityofTraditionalChineseMedicine Nanjing210029JiangsuProvinceChina

AIM:To evaluate the tumor-positive ratio and number of perigastric lymph nodes as prognostic factors of gastric carcinoma in surgically-treated ***:The postoperative survival of 169 patients with gastric cancer who were performed D2 curative gastrectomy was analyzed with regard to its lymph node metastasis ratio and *** correlation of tumor-positive ratio and number of perigastric lymph nodes with pathological parameters of these patients was ***:The overall 5-year survival rate of all the patients studied was 29.6%.The 5-year cumulative survival rate in patients with 1%-20% and more than 20% of tumor-positive lymph nodes was 70.6% and 12.0% respectively,and 46.6% and 17.4% in those with 1-5 and more than 5 of tumorpositive lymph nodes respectively, which were significantly decreased with the increment of involved lymph nodes assessed by either numbers or ratio (P<0.05).Multiple stepwise regression analysis showed that both the positive ratio and number of tumor-involved lymph nodes were sensitive prognostic factors in these surgically-treated patients, which were also significantly correlated with tumor size and depth of submucosal invasion (P<0.05).CONCLUSION:Tumor-positive ratio and number of perigastric lymph nodes are associated with cancer progression and five-year survival rate, and may serve as valuable prognostic factors of gastric cancer in surgically-treated patients.

关键词：胃癌外科治疗胃周淋巴结点预后生存质量

Ultimate Lateral Capacity of Rigid Pile in c–φ Soil

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China Ocean Engineering 2018年第1期32卷 41-50页

作者： ZHANG Wei-min Nanjing Hydraulic Research Institute Nanjing 210029China Key Laboratory of Water Scienceand Engineering Ministry of Water ResourcesNanjing 210029China National Energy Hydropower Engineering Safetyand Environmental Research and Development Center Nanjing 210029China

To date no analytical solution of the pile ultimate lateral capacity for the general c–φ soil has been obtained. In the present study, a new dimensionless embedded ratio was proposed and the analytical solutions of ultimate lateral capacity and rotation center of rigid pile in c–φ soils were obtained. The results showed that both the dimensionless ultimate lateral capacity and dimensionless rotation center were the univariate functions of the embedded ratio. Also,the ultimate lateral capacity in the c–φ soil was the combination of the ultimate lateral capacity（f;） in the clay, and the ultimate lateral capacity（f;） in the sand. Therefore, the Broms chart for clay, solution for clay（φ=0） put forward by Poulos and Davis, solution for sand（c=0） obtained by Petrasovits and Awad, and Kondner’s ultimate bending moment were all proven to be the special cases of the general solution in the present study. A comparison of the field and laboratory tests in 93 cases showed that the average ratios of the theoretical values to the experimental value ranged from 0.85 to 1.15. Also, the theoretical values displayed a good agreement with the test values.

关键词： pile ultimate lateral capacity dimensionless analytical solution c–φ soils hyperbolic function method

Isolation of Nanjing Local Strains of HHV-7 and Their Biological and Immunological Characteristics

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Cellular & Molecular Immunology 2004年第5期1卷 367-372页

作者： KunYao JieDou JianXu HuaSun GuangyongPeng QiangRen XiaohuiJi DepartmentofMicrobiologyandImmunology NanjingMedicalUniversityNanjing210029China.／／Correspondingto：Dr.KunYaoDepartmentofMicrobiologyandImmunologyNanjingMedicalUniversityNanjing210029China. DepartmentofMicrobiologyandImmunology NanjingMedicalUniversityNanjing210029China.

To investigate the biological and immunological characteristics of the Nanjing local strains of HHV-7,four strains of herpesvirus were isolated from saliva specimens of one healthy individual and three children suffering from a kidney disease in *** viruses were identified by transmission electron microscopy (EM),indirect immunofluorescence assay(IFA) with a specific monoclonal antibody;nested polymerase chain reaction,restriction mapping and DNA *** virus-infected cells showed the typical cytophathic effect(CPE) under microscopy and could be detected by IFA with the human herpesvirus-7(HHV-7) specific *** EM,herpesvirus-like and virions capsids could be found in their cytoplasm or nucleoplasm. HHV-7 DNA fragments amplified from infected cells by nested PCR were confirmed by restriction mapping and DNA *** to DC strain,an known HHV-7 strain used in the present study as the positive control,the virus could be inactivated by ultraviolet irradiation for 10 min,heated at 45℃ for 30 min,pH9 at 4℃ for 2 h and ether or chloroform for 10 *** virus induced the production of TNF-α,IL-10 and IL-12p70 while inhibited IFN-γ secretion,increased the percentage of CD2^+ cells while decreased that of CD4^+ or CD45RA^+ *** results indicate that the viruses isolated in Nanjing are HHV-7,which has similar biological characteristic to the known HHV-7 strain,*** with HHV-7 in vitro could affect immune function of lymphocytes by disturbing cytokine production and CD antigen *** & Molecular Immunology.2004;1(5):367-372.

关键词： HHV-7 DC YY

Potential Role of NO in Modulation of COX-2 Expression and PGE2 Production in Pancreatic β-cells

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Acta Biochimica et Biophysica Sinica 2005年第2期37卷 139-146页

作者： Jia-Jian LING Yu-Jie SUN Dong-Ya ZHU Qi CHEN Xiao HAN The Key Laboratory of Human Functional Genomics of Jiangsu Province,Nanjing Medical University,Nanjing 210029,China School of Pharmacy,Nanjing Medical University,Nanjing 210029,China The Key Laboratory of Human Functional Genomics of Jiangsu Province Nanjing Medical UniversityNanjing 210029 China School of Pharmacy Nanjing Medical UniversityNanjing 210029 China

Cytokines have been implicated in pancreatic β-cell destruction leading to type 1 *** to interleuken-1β（IL-1β）of pancreatic β-cells induces expression of inducible nitric oxide syn-thase（iNOS）and cyclooxygenase-2（COX-2）.Subsequent formation of nitric oxide（NO）and prostaglandinE2（PGE2）may impair β-cell *** NOS inhibitor NG-monomethyl-L-arginine（L-NMMA）,wehave further investigated the relation between NO formation and COX-2 ***-1β stimulated theformation of NO and PGE2 by pancreatic β-cells.L-NMMA completely inhibited IL-1β-induced NO forma-tion and attenuated PGE2 ***-2 gene transcription level and protein expression were deter-mined by real-time PCR,Western blot and luciferase analysis.L-NMMA inhibited IL-1β-induced promoteractivity,gene transcription and protein expression of COX-2 in pancreatic β-***,we concludedthat NO-affected COX-2 activity is directly linked to COX-2 gene transcription and protein expression inpancreatic β-*** identification of a novel interaction of NO on the COX signaling pathway in β-cellsprovides a strategy of intervention for further evaluating the role of NO and PGE2 in autoimmune diabetes.

关键词： nitric oxide cyclooxygenase-2 pancreatic β-cell prostaglandin E2