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Pedosphere 2024年第2期34卷 361-373页

作者： Yixuan CHEN Zhonghua WEN Jun MENG Zunqi LIU Jialong WEI Xiyu LIU Ziyi GE Wanning DAI Li LIN Wenfu CHEN national Biochar Institute Shenyang Agricultural UniversityShenyang 110866China key laboratory of Biochar and Soil Improvement Ministry of Agriculture and Rural AffairsShenyang 110866China

Phosphorus(P)is an essential element for plant growth but is often limiting in ecosystems;therefore,improving the P fertilizer use efficiency is *** and arbuscular mycorrhizal fungi(AMF)may enhance P cycling in paddy soils that contain high content of total P but low content of available P(AP).In this study,the effects of biochar addition and Rhizophagus irregularis inoculation on the organic and inorganic P contents and phosphatase activities in paddy soils,rice seedling growth,and AMF colonization were *** with no biochar addition,biochar addition enhanced the percentage of spore germination at day 7,hyphal length,most probable number,and mycorrhizal colonization rate of *** by 32.,662.,70%,and 2.%on average,*** and *** altered soil P cycling and *** and ***,either individually or in combination,increased soil AP content by 2.–48%.Rice seedlings treated with biochar and *** produced greater biomass,improved root morphology,and increased nutrient uptake compared with those of the control without biochar and *** results suggest that combined application of biochar and *** is beneficial to rice cultivation in paddy soils with high content of total P but low content of AP.

关键词： arbuscular mycorrhizal fungi available P phosphorus fraction rice husk biochar seedling growth spore germination

Exploring biofilm-forming molecular determinants in Listeria monocytogenes by comparative genome-wide and transcriptomic analyses

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Food Science and Human Wellness 2024年

作者： Xinyi Zhang Changzheng Shi Zhaoxin Lu Fanqiang Meng Moutong Chen Xiaomei Bie College of Food Science and Technology， Nanjing agricultural University Guangdong Provincial key laboratory of Microbial Safety and Health， State key laboratory of Applied microbiology Southern China， Institute of microbiology， Guangdong Academy of Sciences

In the food industry， bacterial cells usually adhere to equipment surfaces， forming biofilms that may cause persistent contamination. This study aimed to identify the key genes responsible for the stronger biofilm-forming capability of the LMB 3342. strain compared to that of the CICC 2.662.strain through comparative genomics. Additionally， the expression of genes and related metabolic pathways of LMB 3342. and CICC 2.662.strains were analyzed at the transcriptional level by high-throughput sequencing technology to uncover key differentially expressed genes between planktonic and biofilm cells of those two strains. Subsequently， the key genes found to present differences that were uncovered by those genome-wide and transcriptomic analyses were used to construct gene deletion strains. The crystalline violet assay and motility assay showed that GL002.91， GL002.12.and lmo1438 genes were involved in the regulation of biofilm formation as well as motility. The hydrophobicity and auto-aggregation ability assay results demonstrated an association between the clpB， lmo1438， and lmo02.4 genes and bacterial adhesion. However， no significant differences were found regarding this association in the GL002.91 and GL002.12.genes. This study elucidates some potential regulatory genes associated with biofilm formation in L. monocytogenes， and laying a theoretical foundation for future research.

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Streamlined whole-genome genotyping through NGS-enhanced thermal asymmetric interlaced(TAIL)-PCR

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Plant Communications 2024年第9期5卷 25-37页

作者： Sheng Zhao Yue Wang Zhenghang Zhu Peng Chen Wuge Liu Chongrong Wang Hong Lu Yong Xiang Yuwen Liu Qian Qian Yuxiao Chang Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China 2 Guangdong Provincial key laboratory of New Technology in Rice Breeding Rice Research InstituteGuangdong Academy of Agricultural SciencesGuangzhou 510640China

Whole-genome genotyping(WGG)stands as a pivotal element in genomic-assisted plant ***theless,sequencing-based approaches for WGG continue to be costly,primarily owing to the high expenses associated with library preparation and the laborious *** prior development of foreground and background integrated genotyping by sequencing(FBI-seq),we discovered that any sequence-specific primer(SP)inherently possesses the capability to amplify a massive array of stable and reproducible non-specific PCR products across the ***,we further improved FBI-seq by replacing the adapter ligated by Tn5 transposase with an arbitrary degenerate(AD)*** protocol for the enhanced FBI-seq unexpectedly mirrors a simplified thermal asymmetric interlaced(TAIL)-PCR,a technique that is widely used for isolation of flanking ***,the improved TAIL-PCR maximizes the primer-template mismatched annealing capabilities of both SP and AD *** addition,leveraging of next-generation sequencing enhances the ability of this technique to assay tens of thousands of genome-wide loci for any *** cost-effective,user-friendly,and powerful WGG tool,which we have named TAIL-PCR by sequencing(TAIL-peq),holds great potential for widespread application in breeding programs,thereby facilitating genome-assisted crop improvement.

关键词： whole-genome genotyping primer-template mismatched annealing specific primer arbitrary degenerate primer molecular breeding

Characterization of mulberry leaf instant tea and evaluation of its hypolipidemia effect via regulation of intestinal microbiota

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Food Science and Human Wellness 2024年第3期13卷 1348-1357页

作者： Xiaoyun Han Yunlong Bai Xiaoxin Feng Baochang Du Baojiang Zheng Qingshen Sun Engineering Research Center of agricultural microbiology Technology Ministry of Education&Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold RegionKey Laboratory of Molecular Biology&Key Laboratory of MicrobiologyCollege of Heilongjiang Province&School of Life SciencesHeilongjiang UniversityHarbin 150080China Gannan Forest Farm Qiqihaer 162100China College of Life Sciences Northeast Forestry UniversityHarbin 150040China

The purpose of this study was to characterize mulberry leaf instant tea(MLIT)powder prepared from the'Longsang No.1'(Morus abla *** 1)mulberry leaves in Heilongjiang Province(China)and assess its obesity-preventing/relieving effects.A total of 174 compounds including quercetin,chlorogenic acid,1-deoxyecomycin(1-DNJ)related to antihyperlipidemia effects were identified from the MLIT *** treatment reversed the Lee's index,fat coefficient,and serum biochemical parameters in both the obesity relieving and obesity preventing mice fed with high-fat *** the obesity relieving experiment,the relative abundance of Desulfovibrio in mouse feces decreased after both 0.5%and 1%MLIT *** obesity preventing experiments,mouse with different amount of MLIT treatments showed increased relative abundance of Akkermansia,Bifidobacterium and Lactobacillus,while Deferribacteres,Desulfobacterota *** beneficial bacteria in the intestinal tract of mice treated with MLIT *** study proved that MLIT had antihyperlipidemia potential via modulating intestinal microbiota in mice.

关键词： Mulberry leaf instant tea Liquid chromatography-mass spectrometry(LC-MS) Antihyperlipidemia Intestinal microbiota

OsMYB84,a transcriptional regulator of OsCOPT2.and OsHMA5,modulates copper uptake and transport and yield production in rice

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The Crop Journal 2024年第2期12卷 456-469页

作者： Jingli Ding Chenchen Ji Lu Yu Chuang Wang Guangda Ding Sheliang Wang Lei Shi Fangsen Xu Hongmei Cai Research Center of Microelement Huazhong Agricultural UniversityWuhan 430070HubeiChina College of Resources and Environment Huazhong Agricultural UniversityWuhan 430070HubeiChina national key {2. of Crop Genetics and Improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina

Transcription factors regulating crop uptake and translocation of the micronutrient Cu have not been *** isolated a novel R2.3-MYB transcription factor,OsMYB84,and showed that it was a positive regulator involved in uptake and transport of Cu via activation of OsCOPT2.and OsHMA ***84 was highly expressed in roots and anthers and induced by *** of OsMYB84 promoted uptake and root-to-shoot translocation of Cu in rice,facilitated Cu distribution into grain and increased grain *** contrast,mutation of OsMYB84 reduced Cu concentration in xylem ***84 bound to the promoter region of OsCOPT2.and OsHMA5 and upregulated their ***2.mutants showed reduced uptake of Cu and OsHMA5 overexpression lines showed increased root-to-shoot translocation of Cu.

关键词： OsMYB84 OsCOPT2 OsHMA5 Copper Rice

Fine-tuning the spatial allocation of phytohormones for smart canopy, smart breeding, and smart agriculture

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Science China Life Sciences 2024年

作者： Tao Zhou Lin Li national key {2. of Crop Genetic Improvement Huazhong Agricultural University HuBei HongShan laboratory

The dual challenges of global climate change and population growth have increased demand for higher crop yields per unit area. The innovative technology of smart agriculture aims to synergize breeding, cultivation, and production through the application of biological and informatics technologies. Historically, the“Green Revolution” introduced semidwarf varieties of certain key crops by utilizing mutations affecting gibberellin pathways, which significantly increased planting densities and yields.

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The miR159a-DUO1 module regulates pollen development by modulating auxin biosynthesis and starch metabolism in citrus

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Journal of Integrative Plant Biology 2024年第7期66卷 1351-1369页

作者： Yanhui Xu Wenxiu Tian Minqiang Yin Zhenmei Cai Li Zhang Deyi Yuan Hualin Yi Juxun Wu national key {2. for Germplasm Innovation&Utilization of Horticultural Crops Huazhong Agricultural UniversityWuhan 430070China key laboratory of Cultivation and Protection for Non‐Wood Forest Trees of the Ministry of Education and key laboratory of Non‐Wood Forest Products of the Forestry Ministry Central South University of Forestry and TechnologyChangsha 410004China

Achieving seedlessness in citrus varieties is one of the important objectives of citrus *** sterility associated with abnormal pollen development is an important factor in ***,our understanding of the regulatory mechanism underlying the seedlessness phenotype in citrus is still ***,we determined that the miR159a-DUO1 module played an important role in regulating pollen development in citrus,which further indirectly modulated seed development and fruit *** the overexpression of csi-miR159a and the knocking out of DUO1 in Hong Kong kumquat(Fortunella hindsii)resulted in small and seedless fruit ***,pollen was severely aborted in both transgenic lines,with arrested pollen mitotic I and abnormal pollen starch *** additional cross-pollination experiments,DUO1 was proven to be the key target gene for miR159a to regulate male sterility in *** on DNA affinity purification sequencing(DAP-seq),RNA-seq,and verified interaction assays,YUC2.YUC6,SS4 and STP8 were identified as downstream target genes of DUO1,those were all positively regulated by *** transgenic *** lines,the miR159a-DUO1 module down-regulated the expression of YUC2.YUC6,which decreased indoleacetic acid(IAA)levels and modulated auxin signaling to repress pollen mitotic *** miR159a-DUO1 module reduced the expression of the starch synthesis gene SS4 and sugar transport gene STP8 to disrupt starch metabolism in ***,this work reveals a new mechanism by which the miR159a-DUO1 module regulates pollen development and elucidates the molecular regulatory network underlying male sterility in citrus.

关键词： auxin citrus male sterility miRNA pollen development seedlessness starch metabolism

Cytochrome P450 CitCYP97B modulates carotenoid accumulation diversity by hydroxylating b-cryptoxanthin in Citrus

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Plant Communications 2024年第6期5卷 57-72页

作者： Yingzi Zhang Jiajing Jin Nan Wang Quan Sun Di Feng Shenchao Zhu Zexin Wang Shunxin Li Junli Ye Lijun Chai Zongzhou Xie Xiuxin Deng national key {2. for Germplasm Innovation&Utilization of Horticultural Crops College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhan 430070China Hubei Hongshan laboratory WuhanHubei 430070China

Carotenoids in plant foods provide health benefits by functioning as provitamin *** ofthe vital provitamin A carotenoids,b-cryptoxanthin,is typically plentiful in citrus ***,little is known about the genetic basis of b-cryptoxanthin accumulation in ***,we performed a widely targeted metabolomic analysis of 65 major carotenoids and carotenoid derivatives to characterize carotenoid accumulation in Citrus and determine the taxonomic profile of *** used data from 81 newly sequenced representative accessions and 69 previously sequenced Citrus cultivars to reveal the genetic basis of b-cryptoxanthin accumulation through a genome-wide association *** identified a causal gene,CitCYP97B,which encodes a cytochrome P450 protein whose substrate and metabolic pathways in land plants were *** subsequently demonstrated that CitCYP97B functions as a novel monooxygenase that specifically hydroxylates the b-ring of b-cryptoxanthin in a heterologous expression *** planta experiments provided further evidence that CitCYP97B negatively regulates b-cryptoxanthin *** the sequenced Citrus accessions,we found that two critical structural cis-element variations contribute to increased expression of CitCYP97B,thereby altering b-cryptoxanthin accumulation in ***/introgression appear to have contributed to the prevalence of two cis-element variations in different Citrus types during citrus ***,these findings extend our understanding of the regulation and diversity of carotenoid metabolism in fruit crops and provide a genetic target for production of b-cryptoxanthin-biofortified products.

关键词： carotenoids b-cryptoxanthin cytochrome P450 CYP97B hydroxylation

Chronic social defeat stress‑induced depression reduces BCG efficacy by promoting regulatory T‑cell levels in mice

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Animal Diseases 2024年第2期4卷 115-126页

作者： Rohit Tyagi Xi Chen Atika Dhar Bing Yang Wei Zhou Aikebaier Reheman Yingying Lei Gang Cao national key {2. of agricultural microbiology College of Veterinary MedicineHuazhong Agricultural UniversityWuhan 430070China national Institute of Immunology New Delhi 110067India College of Animal Science and Technology Tarim UniversityAlar 843300China Bio‑Medical Center Huazhong Agricultural UniversityWuhan 430070China

Despite the initial successes of the Bacillus Calmette-Guerin(BCG)vaccine in children,its efficacy against tuberculosis is highly *** is a lack of understanding about how mental conditions influence BCG ***,we used the chronic social defeat stress(CSDS)model to explore the effects of depression on BCG vaccination *** observed higher lung and spleen bacterial loads and a lower organ index in depressed compared to BCG ***,a relatively lower T cell protective efficacy was observed in both compared to control and BCG mice via a mycobacterium growth inhibition assay(MGIA).Cytokine expression of IL-12.40,IL-1β,IL-17,TNF-αand IFN-γwas reduced,whereas the expression of IL-10 and IL-5 was increased in the spleen of both compared to BCG ***,the proportions of CD4^(+)IFN-γ^(+),CD8^(+)IFN-γ^(+)T lymphocytes and CD4^(+)effector/central memory T cells were reduced in the splenocytes of the depressed BCG *** promotes CD4^(+)regulatory T cells(Treg)and myeloid-derived suppressor cell(MDSC)generation in depressed mice,contributing to the reduced pro-inflammatory immune response upon BCG *** study provides insight into the decreased protective immunity by BCG vaccination attributable to depression in mice.

关键词： BCG Vaccine efficacy Treg Immune suppression Depression

Molecular Mechanism of Rice Necrotic Lesion for Optimized Yield and Disease Resistance

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Rice science 2024年第3期31卷 285-299页

作者： HOU Xinyue WANG Yuping QIAN Qian REN Deyong State key laboratory of Rice Biology and Breeding China National Rice Research InstituteHangzhou 310006China State key laboratory of Crop Gene Exploration and Utilization in Southwest China Rice Research InstituteSichuan Agricultural UniversityChengdu 611130China

How to balance rice resistance and yield is an important issue in rice *** with mutated necrotic lesion genes often have persistent broad-spectrum resistance,but this broad-spectrum resistance usually comes at the expense of ***,many necrotic lesion mutants in rice have been identified,and these genes are involved in disease resistance *** review provides a detailed introduction to the characteristics,classification,and molecular mechanisms of necrotic lesion ***,we review the molecular regulatory pathways of genes involved in rice disease ***,we summarize the relationship between resistance and yield in rice using newly developed gene editing *** discuss a rational and precise breeding strategy to better utilize molecular design technology for breeding disease-resistant and high-yield rice varieties.

关键词： rice necrotic lesion regulation mechanism high yield high resistance