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Recombinant chitinase-3-like protein 1 alleviates learning and memory impairments via M2 microglia polarization in postoperative cognitive dysfunction mice

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Neural Regeneration Research 2025年第9期20卷 2727-2736页

作者： Yujia Liu Xue Han Yan Su Yiming Zhou Minhui Xu Jiyan Xu Zhengliang Ma Xiaoping Gu Tianjiao Xia Department of Anesthesiology Nanjing Drum Tower HospitalAffiliated Hospital of Medical SchoolNanjing UniversityNanjingJiangsu ProvinceChina Medical school Nanjing UniversityNanjingJiangsu ProvinceChina Jiangsu Key Laboratory of Molecular Medicine Nanjing UniversityNanjingJiangsu ProvinceChina State Key Laboratory of Pharmaceutical biotechnology Nanjing UniversityNanjingJiangsu ProvinceChina Department of Anesthesiology Zhongshan HospitalFudan UniversityShanghaiChina

Postoperative cognitive dysfunction is a seve re complication of the central nervous system that occurs after anesthesia and surgery,and has received attention for its high incidence and effect on the quality of life of *** date,there are no viable treatment options for postoperative cognitive *** identification of postoperative cognitive dysfunction hub genes could provide new research directions and therapeutic targets for future *** identify the signaling mechanisms contributing to postoperative cognitive dysfunction,we first conducted Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses of the Gene Expression Omnibus GSE95426 dataset,which consists of mRNAs and long non-coding RNAs differentially expressed in mouse hippocampus3 days after tibial *** dataset was enriched in genes associated with the biological process"regulation of immune cells,"of which Chill was identified as a hub ***,we investigated the contribution of chitinase-3-like protein 1 protein expression changes to postoperative cognitive dysfunction in the mouse model of tibial fractu re *** were intraperitoneally injected with vehicle or recombinant chitinase-3-like protein 124 hours post-surgery,and the injection groups were compared with untreated control mice for learning and memory capacities using the Y-maze and fear conditioning *** addition,protein expression levels of proinflammatory factors(interleukin-1βand inducible nitric oxide synthase),M2-type macrophage markers(CD206 and arginase-1),and cognition-related proteins(brain-derived neurotropic factor and phosphorylated NMDA receptor subunit NR2B)were measured in hippocampus by western *** with recombinant chitinase-3-like protein 1 prevented surgery-induced cognitive impairment,downregulated interleukin-1βand nducible nitric oxide synthase expression,and upregulated CD206,arginase-1,pNR2B,and brain-derived neurotropic factor expr

关键词： Chil1 hippocampus learning and memory M2 microglia neuroinflammation postoperative cognitive dysfunction(POCD) recombinant CHI3L1

Evaluation of Bacillus sp. MZS10 for decolorizing Azure B dye and its decolorization mechanism

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Journal of Environmental Sciences 2014年第5期26卷 1125-1134页

作者： Huixing Li Ruijing Zhang Lei Tang Jianhua Zhang Zhonggui Mao The Key Laboratory of Industrial biotechnology Ministry of Education School of Biotechnology Jiangnan University school of Biological and Chemical Engineering Nanyang Institute of Technology

To evaluate decolorization and detoxification of Azure B dye by a newly isolated Bacillus sp. MZS 10 strain, the cultivation medium and decolorization mechanism of the isolate were investigated. The decolorization was discovered to be dependent on cell density of the isolate and reached 93.55% （0.04 g/L） after 14 hr of cultivation in a 5 L stirred-tank fermenter at 2.0 g/L yeast extract and 6.0 g/L soluble starch and a small amount of mineral salts. The decolorization metabolites were identified with ultra performance liquid chromatography-tandem mass spectroscopy （UPLC-MS）. A mechanism for decolorization of Azure B was proposed as follows： the C=N in Azure B was initially reduced to -NH by nicotinamide adenine dinucleotide phosphate （NADPH）-dependent quinone dehydrogenase, and then the -NH further combined with -OH derived from glucose to form a stable and colorless compound through a dehydration reaction. The phytotoxicity was evaluated for both Azure B and its related derivatives produced by Bacillus sp. MZS 10 decolorization, indicating that the decolorization metabolites were less toxic than original dye. The decolorization efficiency and mechanism shown by Bacillus sp. MZS10 provided insight on its potential application for the bioremediation of the dye Azure B.

关键词： Azure B Bacillus sp. decolorization ultra performance liquid chromato graphy-tandem mass spectroscopy phytotoxicity

DESIGN AND PHOTOFUNCTIONS OF DENDRIMER-ENCAPSULATED POLY(PHENYLENEETHYNYLENE)S

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Chinese Journal of Polymer Science 2001年第2期19卷 161-166页

作者： Takafumi Sato Takuzo Aida Department of Chemistry and biotechnology Graduate School of Engineering The University of Tokyo 7-3-1 Hongo Bunkyo-ku Tokyo 113-8656 JapanDepartment of Chemistry and Biotechnology Graduate School of Engineering The University of Tokyo 7-3-1 Hongo Bunkyo-ku Tokyo 113-8656 Japan

A series of dendrimer-encapsulated poly(phenyleneethynylene)s 4～6 were Synthesize d. The light-harvesting antenna functions of dendrimer frameworks together with the blue-light emitting activities of 4～6 were highli... 详细信息

关键词： Dendrimer Poly(phenyleneethynylene) Molecular wire Light-harvesting antenna Energy transfer

De novo synthesis of atropisomeric benzofurans via Cu/SPDO complex catalyzed asymmetric formal [3 + 2] annulation

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Science China Chemistry 2025年第1期 174-180页

作者： Fan-Xiao Meng Xing-Yu Wang Ka Lu Ju-Song Yang Fu-Min Zhang Ai-Jun Ma Bao Du Yong-Qiang Tu State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical Engineering Lanzhou University school of Chemistry and Chemical Engineering Shanghai Jiao Tong University school of biotechnology and Health Sciences Wuyi University

Herein, the de novo synthesis of atropisomeric benzofurans through the Cu/spirocyclic-pyrrolidine-oxazoline(SPDO) complex catalyze asymmetric formal [3 + 2] cycloaddition between quinones and ynamides is explored, producing a range of structurally diverse axially chiral benzofurans in satisfactory yields with good to excellent enantioselectivities. Furthermore, density functional theory calculations were applied to explain the possible reaction mechanism.

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Reconstruction of tyrosol synthetic pathways in Escherichia coli

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Chinese Journal of Chemical Engineering 2018年第12期26卷 2615-2621页

作者： Cui Yang Xianzhong Chen Junzhuang Chang Lihua Zhang Wei Xu Wei Shen You Fan Key Laboratory of Carbohydrate Chemistry & biotechnology Ministry of Education Jiangnan University school of biotechnology Jiangnan University

Tyrosol is a pharmacologically active phenolic compound widely used in the medicine and chemical *** methods of plant extraction are complicated and chemical synthesis of tyrosol is not commercially viable. In this study, a recombinant Escherichia coli strain was constructed by overexpressing the phenylpyruvate decarboxylase ARO10 from Saccharomyces cerevisiae, which could produce tyrosol from glucose. Furthermore,genes encoding key enzymes from the competing phenylalanine and tyrosine synthesis pathways and the repression protein TyrR were eliminated, and the resulting engineered strain generated 3.57 mmol·L^(-1) tyrosol from glucose. More significantly, codon optimization of ARO10 increased expression and tyrosol titer. Using the novel engineered strain expressing codon-optimized AR10 in shake-flask culture, 8.72 mmol·L^(-1) tyrosol was obtained after 48 h. Optimization of the induction conditions improved tyrosol production to 9.53 mmol·L^(-1)(1316.3 mg·L^(-1)). A higher titer of tyrosol was achieved by reconstruction of tyrosol synthetic pathway in E. coli.

关键词： Tyrosol Escherichia coli Phenylpyruvate decarboxylase Gene knockout Codon optimization

Changes in endogenous hormone levels and redox status during enhanced adventitious rooting by rare earth element neodymium of Dendrobium densiflorum shoot cuttings

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Journal of Rare Earths 2008年第6期26卷 869-874页

作者：罗建平张竞成王瑛 school of biotechnology and Food Engineering Hefei University of Technology

The effects of neodymium nitrate （Nd^3＋） on the adventitious rooting of Dendrobium densiflorum shoot cuttings were studied. The addition of Nd^3＋（5 μmol/L） to culture medium significantly increased rooting frequency. Histological investigation showed that Nd^3＋ did not change the process of root initiation. Nd^3＋ did not influence total endogenous cytokinin levels, but significantly increased the level of endogenous indole-3-acetic acid （IAA） in the base of shoot cuttings. Compared to the control, the ratio of IAA/cytokinins was very high in the Nd^3＋ treatment. These results suggested that the enhanced rooting frequency may be related to the increase in endogenous IAA level in Nd^3＋ treatment. Analysis of enzyme activities showed that the enhanced accumulation of the endogenous IAA by Nd^3＋ should not be attributed to inhibition of IAA decomposition by IAA oxidase or promotion of cytokinin decomposition by cytokinin oxidase. Besides, Nd^3＋ increased the ratio of reduced glutathione （GSH） and oxidized glutathione （GSSG） in the process of adventitious rooting while the ratio of ascorbate （ASC） to dehydroascorbate （DHA） was not affected.

关键词： Dendrobium densiflorum neodymium nitrate adventitious rooting indole-3-acetic acid cytokinins rare earths

Recent progress of on-resin cyclization for the synthesis of clycopeptidomimetics

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Chinese Chemical Letters 2016年第12期27卷 1731-1739页

作者： Zhi-Meng Wu Shao-Zhong Liu Xiao-Zhong Cheng Wen-Zhang Ding Tao Zhu Bing Chen The Key Laboratory of Carbohydrate Chemistry & biotechnology Ministry of Education School of Biotechnology Jiangnan University

Cyclopeptidomimetics are class of cyclopeptides with unnatural linkage. They usually displayed unique constrained structure, enhanced proteolytic stability, and other drug-like character; and have been widely used in medicinal chemistry. Therefore, development of efficient strategies for the synthesis of cyclopeptidomimetics has received many attentions. On-resin cyclization strategy is one of the effective approaches developed to overcome the competing side reaction such as oligomerization and cyclooligomers occurred in solution cyclization. This approach took advantage of the ＂pseudo-dilution＂ effect to avoid these undesired by-products and greatly simplified the downstream product purification process. This review summarized the recent on-resin peptide cyclization strategies for the synthesis of cvclooeotidomimetics.

关键词： Cyclopeptidomimetics On-resin Cyclization Synthesis

Insight into the distribution of metallic elements in membrane bioreactor: Influence of operational temperature and role of extracellular polymeric substances

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Journal of Environmental Sciences 2019年第2期31卷 111-120页

作者： He Wang Xiufen Li Xinhua Wang Yueping Ren Laboratory of Environmental biotechnology School of Environmental and Civil EngineeringJiangnan University Jiangsu Key Laboratory of Anaerobic biotechnology

The distribution of metallic elements in a submerged membrane bioreactor(MBR) was revealed at different temperatures using inductively coupled plasma-optical emission spectrometry(ICP-OES), and the role of extracellular polymeric substances(EPS) was probed by integrating scanning electron microscopy(SEM) with confocal laser scanning microscopy(CLSM) over long-term operation. More metallic elements in the influent were captured by suspended sludge and built up in the fouling layer at lower temperature. The concentration of metallic elements in the effluent was 5.60 mg/L at 10°C operational temperature, far lower than that in the influent(51.35 mg/L). The total contents of metallic elements in suspended sludge and the membrane fouling layer increased to 40.20 and 52.19 mg/g at 10°C compared to 35.14 and 32.45 mg/g at 30°C, and were dominated by the organically bound fraction. The EPS contents in suspended sludge and membrane fouling layer sharply increased to 37.88 and 101.51 mg/g at 10°C, compared to 16.87 and 30.03 mg/g at 30°C. The increase in EPS content at lower temperature was responsible for the deposition of more metallic ions. The strong bridging between EPS and metallic elements at lower temperature enhanced the compactness of the fouling layer and further decreased membrane flux. This was helpful for understanding the mechanism of membrane fouling at different operational temperatures and the role of EPS, and also of significance for the design of cleaning strategies for fouled membranes after long-term operation.

关键词： Membrane bioreactor Metallic elements Extracellular polymeric substances

Recent advances in synthetic carbohydrate-based human immunodeficiency virus vaccines

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Virologica Sinica 2016年第2期31卷 110-117页

作者： Zhenyuan Wang Chunjun Qin Jing Hu Xiaoqiang Guo Jian Yin Key Laboratory of Carbohydrate Chemistry and biotechnology Ministry of Education School of Biotechnology Jiangnan University Wuxi Medical school Jiangnan University

An effective vaccine for human immunodeficiency virus(HIV) is urgently needed to prevent HIV infection and progression to acquired immune deficiency syndrome(AIDS). As glycosylation of viral proteins becomes better understood, carbohydrate-based antiviral vaccines against special viruses have attracted much attention. Significant efforts in carbohydrate synthesis and immunogenicity research have resulted in the development of multiple carbohydrate-based HIV vaccines. This review summarizes recent advances in synthetic carbohydrate-based vaccines design strategies and the applications of these vaccines in the prevention of HIV.

关键词： vaccines carbohydrate advances antiviral epitope vaccination antiretroviral antigenic prevention understood