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Cochlear implants in genetic deafness

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Journal of Otology 2014年第4期9卷 156-162页

作者： Xuezhong Liu department of otolaryngology University of Miami Miller School of Medicine

Genetic defects are one of the most important etiologies of severe to profound sensorineural hearing loss and play an important role in determining cochlear implantation *** the pathogenic mutation types of a number of deafness genes have been cloned,the pathogenesis mechanisms and their relationship to the outcomes of cochlear implantation remain a hot research *** auditory performance is considered to be affected by the etiology of hearing loss and the number of surviving spiral ganglion cells,as well as *** research advances in cochlear implantation for hereditary deafness,especially the relationship among clinic-types,genotypes and outcomes of cochlear implantation,will be discussed in this review.

关键词： Connexin Cx26/Cx30 Usher syndrome Mitochondria genes Waardenburg syndrome Auditory neuropathy

EFFECT OF LIPOSOMAL TRANSFECTION OF HTERT ON APOPTOSIS OF NEWBORN RAT COCHLEAR BASILAR MEMBRANE CELLS

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Journal of Otology 2012年第2期7卷 105-108页

作者： XU YInglong LIU Hui WANG Junli XU Min department of otolaryngology the second Affiliated HospitalMedical School of Xi'an Jiaotong University department of otolaryngology Shaanxi Provincial People'sHospital

Objective The aim of this study is to investigate the effect of transfected hTERT gene on cell apoptosis of newborn rat cochlear basilar membrane cells (CBMCs). Methods CBMCs isolated from newborn rat cochlear were transfected using a plasmid containing human telomerase reverase transcriptase gene (pCI-neo-hTERT) , and were screened using G418 to obtain stable transfected cell lines. Cell apoptosis rate was analyzed by flow cytometry. hTERT and apoptosis related genes expression were detected by reverse transcriptase polymerase chain reaction (RT-PCR). Results hTERT gene expression was detected 72 hours after gene transfection in transfected cells. The apoptotic rate of transfected CBMCs significantly reduced. Expression of apoptosis related genes correspondingly changed. Conclusion Transfection of hTERT gene leads to reduced apoptosis rate in newborn rat *** lower expression of apaf1, Caspase3 and BCL2 in transfected cells as compared to that of normal CBMCs.

关键词： cochlear basilar membrane cel(lCBMC) hTERT rat apoptosis

GROWTH INHIBITION OF HUMAN LARYNGEAL CANCER CELL WITH THE ADENOVIRUS-MEDIATED p53 GENE

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Chinese Journal of Cancer Research 1999年第3期11卷 157-160页

作者：王琪韩德民王文革吴祖泽张伟 department of otolaryngology-Head and Neck Surgery Institute of Otolaryngology Beijing Tongren Hospital Capital University of Medical Sciences Beijing China

Objective: In most laryngeal cancers, the function of p53 gene is down regulated. To explore the potential use of p53 in gene therapy of laryngeal cancer, by introducing wild-type p53 into laryngeal cancer cell line via a recombinant adenoviral vector, Ad5CMV-p53 and analyzing its effects on cell and tumor growth. Methods: A human laryngeal cancer, cell line Hep-2 was used. Recombinant cytomegalovirus-promoted adenoviruses containing human wild-type p53 cDNA was transiently introduced into Hep-2 line. The growth suppression of the Hep-2 cells and established s.c. squamous, carcinoma model was examined. The p53 protein expression was detected using immunohistochemical analysis. Results: The transduction efficiencies of Hep-2 cell line were 100% at a multiplicity of 100 or greater. The p53 protein expression peaked on day 2 after infection and lasted far 5 days. In vitro growth assays revealed cell death following Ad5CMV-p53 infected. In vivo studies, Ad5CMV-p53 inhibited the tumorigenicity of Hep-2 cell, and in nude mice with established s.c. squamous, carcinoma, nodules showed that tumor volumes were significantly reduced in mice that received peritumoral infiltration of Ad5CMV-p53. Conclusion: Adenovirus-mediated antitumor therapy carrying the p53 gene is an efficient method to inhibit laryngeal cancer growth. Transfection of laryngeal cancer cells with the wild-type p53 gene via Ad5CMV-p53 is a potential novel approach to the therapy of laryngeal cancer.

关键词： Gene therapy Laryngeal cancer p53 gene Adenoviruses

Inflammatory responses to Hydroxyapatite implants in middle ear in rats

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Journal of Otology 2008年第1期3卷 24-28页

作者： YE Qing 1, 2, JIANG Yi 1, 2, WANG Xiao-yan1, 2, ZHENG Ke-fei 1, 2 1 department of otolaryngology, Fujian Provincial Hospital, Fuzhou 350001, China 2 department of otolaryngology, Fujian Medical University, Fuzhou 350001, China department of otolaryngology Fujian Provincial Hospital Fuzhou 350001 China department of otolaryngology Fujian Medical University Fuzhou 350001 China

Objective To study local inflammatory response after implantation of hydroxyapatite synthetic ossicular prosthesis. Methods Hydroxyapatite granules were implanted in the bulla in 32 rats. Sham surgical procedures were performed in 10 rats as the control. Animals were sacrificed at 1 to 300 days after surgery. Bulla sections, stained with HE and Mallory’s azan, were examined for numbers and percentages of various inflammatory cell types. Results Slightly more inflammatory reaction was seen in animals with the implant than in the controls, mostly during the early stage following the implantation procedure. Few inflammatory cells were observed at later times. There were satisfactory fibrosis in both implanted and control ears. Conclusion The results indicate that hydroxyapatite synthetic prosthesis is a biocompatible implantation material in the middle ear. Nonetheless, the presence of inflammatory reaction immediately following implantation implies that control of infection is important in the early times after the implantation procedure.

关键词： Middle ear Hydroxyapatite Synthetic auditory prosthesis inflammatory response

Safety and outcomes of cochlear implantation in the elderly:A review of recent literature

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Journal of Otology 2016年第1期11卷 1-6页

作者： Zao Yang Maura Cosetti department of otolaryngology-Head and Neck Surgery Louisiana State University Health

Global demographic changes related to longevity are leading to increasing numbers of the elderly, for whom hearing loss is a significant cause of morbidity and disability. Once met with reticence, severely hearing impaired older adults are increasingly being considered for cochlear implantation (CI). Significant data indicate that CI in the elderly population is safe, well-tolerated, and effective. Risks from CI surgery and anesthesia are low and generally comparable to rates in other age groups. Outcomes studies regarding CI in older adults have shown excellent improvements to speech perception, quality of life, and even cognition. Overall, currently available data suggests that advanced age should not, in itself, be considered a barrier to implantation. This review paper will highlight selected articles from recent medical literature regarding the safety and efficacy of CI in the elderly population. Copyright ? 2016, PLA General Hospital department of otolaryngology Head and Neck Surgery. Production and hosting by Elsevier (Singapore) Pte Ltd. This is an open access article under the CC BY-NC-ND license (http://***/licenses/by-nc-nd/4.0/).

关键词： Cochlear implants Elderly Hearing loss Safety Efficacy

Stathmin as a surrogate marker of phosphatidylinositol-3-kinase pathway activity:Towards precision medicine in HPV-negative head&neck squamous cell carcinoma

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Genes & Diseases 2022年第3期9卷 820-825页

作者： Andrew T.Turk Dario Garcia-Carracedok David T.Kent Elizabeth Philipone Juana Maria Garcia-Pedrero Salvatore M.Caruana Lanny G.Close Gloria H.Su department of Pathology and Cell Biology and Columbia UniversityNew YorkNY 10032USA department of otolaryngology Columbia UniversityNew YorkNY 10032USA department of otolaryngology Vanderbilt UniversityNashvilleTN 37232USA department of otolaryngology Hospital Universitario Central de Asturias and Instituto Universitario de Oncologia Del Principado de AsturiasOviedo 33006Spain

In order to assess Stathmin as an immunohistochemical(IHC)indicator of phosphatidylinositol 3-kinase(PI3K)pathway activity in HPV-negative head&neck squamous cell carcinoma(HNSCC),we compared Stathmin IHC to expression of other pathway *** also evaluated the relationship between Stathmin IHC and the mutational status of four key pathway ***,we ascertained whether Stathmin IHC correlates with tumor grade or primary *** exists between high Stathmin expression and high pAKT1 expression,indicating a role for Stathmin IHC as a marker of pathway *** analysis did not show correlation between Stathmin IHC and mutation of the four genes *** also observed an association between high Stathmin expression and oropharyngeal primary *** results suggest utility of Stathmin IHC as an indicator of PI3K pathway activity,and thereby demonstrate potential relevance of Stathmin IHC in the context,of HNSCC.

关键词： Cancer Head&neck squamous cell carcinoma Pathology Stathmin

The clinical association of programmed cell death protein 4(PDCD4) with solid tumors and its prognostic significance:a meta-analysis

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Chinese Journal of Cancer 2016年第12期35卷 683-698页

作者： John Zeng Hong Li Wei Gao Wai-Kuen Ho Wen Bin Lei William Ignace Wei Jimmy Yu-Wai Chan Thian-Sze Wong department of Surgery The University of Hong KongQueen Mary Hospital department of otolaryngology The First Affiliated Hospital of Sun Yat?sen University

Background: Programmed cell death protein 4(PDCD4) is a novel tumor suppressor protein involved in pro?grammed cell death. Its association with cancer progression has been observed in multiple tumor models, but evidence supporting its association with solid tumors in humans remains controversial. This study aimed to determine the clinical signiicance and prognostic value of PDCD4 in solid ***: A systematic literature review was performed to retrieve publications with available clinical informa?tion and survival data. The eligibility of the selected articles was based on the criteria of the Dutch Cochrane Centre proposed by the Meta?analysis Of Observational Studies in Epidemiology group. Pooled odds ratios(ORs), hazard ratios(HRs), and 95% conidence intervals(CIs) for survival analysis were calculated. Publication bias was examined by Begg's and Egger's ***: Clinical data of 2227 cancer patients with solid tumors from 23 studies were evaluated. PDCD4 expression was signiicantly associated with the diferentiation status of head and neck cancer(OR 4.25, 95% CI 1.87–9.66) and digestive system cancer(OR 2.87, 95% CI 1.84–4.48). Down?regulation of PDCD4 was signiicantly associated with short overall survival of patients with head and neck(HR: 3.44, 95% CI 2.38–4.98), breast(HR: 1.86, 95% CI 1.36–2.54), digestive system(HR: 2.12, 95% CI 1.75–2.56), and urinary system cancers(HR: 3.16, 95% CI 1.06–9.41).Conclusions: The current evidence suggests that PDCD4 down?regulation is involved in the progression of several types of solid tumor and is a potential marker for solid tumor prognoses. Its clinical usefulness should be conirmed by large?scale prospective studies.

关键词： Programmed cell death protein 4(PDCD4) Solid tumor Meta-analysis Prognosis Overall survival Disease-free survival Recurrence-free survival

Dual Convergence of Facial Nerve Branches Innervating Whisker Pad in Rats

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Current Medical Science 2018年第6期38卷 982-988页

作者： Lin-li YAO E ZHANG Chun-li LI Qi YAO Chang HUANG Xi HUANG Pei CHEN department of otolaryngology Wuhan Hospital of Traditional Chinese and Western Medicine (Wuhan No.1 Hospital)Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China

The precise anatomy of the facial nerve branches innervating rat whisker pad and the distribution of their corresponding motor neurons in facial nucleus area were *** extratemporal facial nerves of 6 rats were anatomically observed under a surgical microscope,and then the nerve specimens of facial nerve branches at 7 anatomical sites were taken and examined for the axons and myelin sheath using Luxol fast blue *** distribution of facial motor neurons innervating the facial branches was observed in 12 rats by retrograde *** distal pes,a fusing architecture of the buccal and marginal mandibular branches,was found to furcate into superior,middle and inferior branches to innervate whisker ***,the myelin sheath of each branch was morphologically consistent,and the nerve fiber bundles of facial nerve branches became increasingly thinner and scattered,particularly after crossing the distal pes site and innervating the whisker *** facial motor neurons innervating the buccal and marginal mandibular branches were clearly distributed in similar regions in facial *** study confirmed the highly spatial synergy between the buccal and marginal mandibular branches innervating the whisker pad from extratemporal anatomy and distribution of facial motor neurons.

关键词： facial nerve whisker pad anatomy facial nucleus rats

Autoinflammatory characteristics and short-term effects of delivering high-dose steroids to the surface of the intact endolymphatic sac and incus in refractory Ménière’s disease

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Journal of Otology 2019年第2期14卷 40-50页

作者： Jing Zou department of otolaryngology-Head and Neck Surgery Center for Otolaryngology-Head & Neck Surgery of Chinese PLA Changhai Hospital Second Military Medical University

Objective:To investigate immune-related genetic background in intractable Meniere’s disease(MD)and the immediate results of a novel therapy by delivering steroids to the surface of the intact endolymphatic sac(ES)and incus in a sustainable *** report and methods:Candidate genes involved in immune regulation were sequenced using a nextgeneration sequencing method in a patient with intractable *** were confirmed using the Sanger sequencing *** ES was exposed,and gelatin sponge particles were immersed in highdose methylprednisolone solution and placed onto the surface of ES.“L”-shaped gelatin sponge strips were immersed in dexamethasone solution and served as a guiding device for the steroids by touching the incus and gelatin sponge particles on the surface of the *** sponge particles immersed in dexamethasone solution were placed around the gelatin sponge strips and sealed using fibrin ***:Autoinflammation in the refractory MD case was indicated by genotype,including novel heterozygous mutations of PRF1,UNC13D,SLC29A3,ITCH,and JAK3,as well as *** vertigo was fully relieved immediately after *** and aural fullness were resolved 3 weeks after operation,whereas hearing improved in 2 mon *** recurrence was noted during the 5-monfollow-up,and the final MRI supported the novel therapeutic ***:Autoinflammation was involved in a refractory *** novel therapy,which involves the delivery of steroids to the surface of the intact ES and incus,is effective in relieving vertigo and tinnitus and improves hearing function of refractory MD.

关键词： Vestibular function Tinnitus Genetic disorders Hearing