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World Journal of Gastroenterology 2004年第10期10卷 1533-1536页

作者： JunCui Bao-WeiDong PingLiang Xiao-LingYu De-JiangYu DepartmentofUltrasound ChinesePLAGeneralHospitalBeijing100853China

AIM: To explore the effect of c-myc, Ki-67, MMP-2 and VEGF expression on prognosis of hepatocellular carcinoma (HCC) patients undergoing tumor ***: Primary HCC patients underwent tumor resection were retrospectively analysed. The maximum size of the tumor was less than 5 cm, there was only one nodule in each patient. No chemoembolization was performed before resection. They were followed up after resection, and the time of recurrence was recorded. They were divided into 2 groups: group A (15 cases): tumor recurrence within 1 year after tumor resection, and group B (15 cases):with or without tumor recurrence 2 years after tumor resection. Pathological slices were made with tumor waxsample. Immunohistochemistry staining was performed with c-myc, Ki-67, MMP-2 and VEGF monoclonal *** intensity was quantitatively analysed with a pathological diagram-writing analyzing system. The expressing intensity differences of stained molecules in cancer tissue and para-cancer were ***: c-myc, Ki-67, MMP-2 and VEGF expressing intensities in cancer tissue in group A were higher than those in group B (Pvalues were 0.010, 0.030, 0.022 and 0.004, respectively), but they were not significantly different in para-cancer tissue in groups A and B (Pvalues were 0.334, 0.343, 0.334 and 0.334, respectively).CONCLUSION: The expression of c-myc, Ki-67, MMP-2 and VEGF in cancer tissue is related to the recurrence of HCC after tumor resection.

关键词：肝细胞癌肿瘤切除术预后肿瘤标志物

Outcomes of loco-regional therapy for down-staging of hepatocellular carcinoma prior to liver transplantation

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Hepatobiliary & Pancreatic Diseases International 2011年第2期10卷 143-150页

作者： Xian-Jie Shi, Xin Jin, Mao-Qiang Wang, Li-Xin Wei, Hui-Yi Ye, Yu-Rong Liang, Ying Luo and Jia-Hong DongDepartment of Hepatobiliary Surgery Department of Intervention Radiology Department of Pathology and Department of Radiology General Hospital of PLA, Beijing 100853, China Department of Hepatobiliary Surgery General Hospital of PLA Beijing 100853 China Department of Intervention Radiology General Hospital of PLA Beijing 100853 China Department of Pathology General Hospital of PLA Beijing 100853 China Department of Radiology General Hospital of PLA Beijing 100853 China

BACKGROUND: The number of loco-regional therapies (LRTs) for hepatocellular carcinoma (HCC) has increased dramatically during the past decade. Many patients with HCC who were beyond the Milan criteria were allowed to receive a liver transplantation (LT) once the HCC was successfully down-staged. This retrospective study aimed to analyze the outcomes of LRTs prior to LT in patients with HCC beyond the Milan criteria. METHODS: We analyzed 56 patients treated from June 2006 to March 2010: 22 met the Milan criteria (T1+T2, 39.3%), 16 had T3 tumors (28.6%), and 11 had T4a tumors (19.6%), while 7 were suspected of tumor vascular invasion (T4b, 12.5%). All patients underwent preoperative LRTs, including transcatheter arterial chemoembolization, radiofrequency ablation, percutaneous ethanol injection, liver resection, and/or microwave coagulation therapy. The number of the patients who were successfully down-staged before LT, the types of LRTs used before LT, and their outcomes after LT were recorded. RESULTS: Eleven patients had necrotic tumors (pT0, 19.6%); 6 had pT1 tumors (10.7%), 22 had pT2 tumors (39.3%), 6 had pT3 tumors (10.7%), 5 had pT4a tumors (8.9%), and 6 had pT4b tumors (10.7%). The histopathologic tumors of 39 patients (69.6%) were down-staged and met the established Milan criteria (pT0-2). Imaging-proven under-staging was present in 5 HCC patients (8.9%) who had tumors involving the intrahepatic venous system. Twenty-three patients (41.1%) had stable HCC and 10 (17.9%) died. The 1-, 3- and 4-year survival rates were 96%, 73% and 61%, respectively, with a mean survival time of 22.29±1.63 months. Six patients died of tumorrecurrence. The 1-, 3- and 4-year recurrence-free survival (RFS) rates were 88%, 75% and 66%, respectively. The 3-year RFS of patients with pT0-2 tumors was 82%, which was markedly greater than that of patients with pT3 tumors (63%, P=0.018) or pT4 tumors (17%, P=0.000). Although the 3-year RFS of patients with pT3 tumors was greater than tha

关键词： hepatocellular carcinoma tumor down-staging loco-regional therapy liver transplantation

Protective Effects of Anthocyanins Extracted from Vaccinium Uliginosum on 661W Cells Against Microwave-Induced Retinal Damage

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Chinese Journal of Integrative Medicine 2022年第7期28卷 620-626页

作者： YIN Lan FAN Si-jun ZHANG Mao-nian Department of Ophthalmology Chinese PLA General HospitalBeijing 100853China Department of Ophthalmology PLA Rocket Characteristic Medical CenterBeijing 100853China

Objective: To study the protective effect of anthocyanins extracted from Vaccinium Uliginosum(VU)on retinal 661W cells against microwave radiation induced retinal injury. Methods: 661W cells were divided into 6 groups, including control, model [661W cells radiated by microwave(30 mW/cm2, 1 h)] and VU groups [661W cells pretreated with anthocyanins extracted from VU(25, 50, 100 and 200 μg/mL, respectively) for 48 h, and radiated by microwave 30 mW/cm2, 1 h]. After treatment with different interventions, the cell apoptosis index(AI)was determined using Heochst staining;contents of malonaldehyde(MDA), glutataione(GSH), and activity of superoxide dismutase(SOD) were measured. mRNA expressions of nuclear factor erythroid 2-related factor 2(Nrf2) and heme oxygenase 1(HO-1) were detected by real time quantitative polymerase chain reaction, and the expression of HO-1 protein was examined by Western blot analysis. Nucleus and cytoplasm were separated and Nrf2 protein expression was further verified by Western blot analysis. Results: There was significant difference in AI among the groups(F=322.83, P<0.05). Compared with the control group, AI was significantly higher in the model group and was lower in 4 VU-pretreated groups(P<0.05). Linear regression analysis showed the decline of AI was in a dose-dependent manner with VU treatment(r=0.8419, P<0.05). The MDA and GSH contents of 661W cells in VU-treated groups were significantly lower than the model group(P<0.05). Compared with the model group, the SOD activity in the VU-treated groups(50, 100 and 200 μg/mL) was significantly higher(all P<0.05). The Nrf2 and HO-1 mRNA expressions were slightly increased after irradiation, and obviously increased in 100 μg/mL VU-treated group. After irradiation, the relative expressions of HO-1 and Nrf2 proteins in nucleus were slightly increased(P<0.05), and the changes in cytoplasm were not obvious,whereas it was significantly increased in both nucleus and cytoplasm in the VU treatment group

关键词： Vaccinium Uliginosum anthocyaninsm photoreceptor microwave apoptosis nuclear factor erythroid 2-related factor 2/heme oxygenase 1

Detection of K-ras gene mutation in fecal samples from elderly large intestinal cancer patients and its diagnostic significance

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World Journal of Gastroenterology 2004年第5期10卷 743-746页

作者： JunWan Zi-QiZhang Wei-DiYou Hua-KuiSun Jian-PingZhang Ya-HongWang Yong-HeFu DepartmentofGeriatricGastroenterology GeneralHospitaloftheChinesePLABeijing100853China

AIM:To study the diagnostic significance of K-ras gene mutations in fecal samples from elderly patients with large intestinal ***: DNA was extracted in the fecal and tissue samples from 23 large intestinal cancer patients, 20 colonic adenomatoid polypus patients and 20 healthy subjects. The K-ras gene mutations at the first and second bases of codon 12 were detected by the allele specific mismatch ***: The K-ras gene mutation was 56.52%(13/23) in the large intestinal cancer patients, which was notably higher than that in the normal subjects whose K-ras gene mutation was 5%(1/20) (x^2=12.93, P0.05). The K-ras gene mutation at the second base of codon 12 was 92.13%(12/13) in the large intestinal cancer patients. There was no significant difference between the detection rate of K-ras gene mutation in the fecal and tissue samples (X^2=9.35, P<0.01).CONCLUSION:Our results indicate that detection of the K-ras gene mutations in fecal samples provides a non-invasive diagnostic method for the elderly large intestinal cancer patients. Its significance in the early diagnosis of large intestinal cancer awaits further studies.

关键词： K-ras基因肠道癌肿瘤诊断学粪便标本基因错配聚合酶链反应

PET imaging of cerebral metabolic change in tinnitususing ^(18)F-FDG

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Nuclear Science and Techniques 2000年第4期11卷 247-252页

作者： TIAN Jia-He, WANG Hong-Tian, YIN Da-Yi, YAO Shu-Lin, SHAO Ming-Zhe, YANG Wei-Yan, JIANG Si-Chang (The PLA General Hospital, Beijing 100853) 解放军总医院北京 100853

Tinnitus is an auditory disorder hardly assessable by clinical technology. PET imaging of the brain in 13 cases with and 10 without tinnitus was undertaken at 40 min after injection of 280-440 MBq 18F-FDG. To ensure the quality of the PET study, all cases followed a normalized procedure with visual and auditory blockage. CT/MRI imaging and routine acoustic tests were carried out in all subjects. PET revealed that an increased uptake of 18F-FDG at left med-temporal lobe (primary auditory center, PAC) present exclusively in tinnitus, regardless the side of hearing hallucination. Significant asymmetry was noted between left and right PAC, but not at other cortex area. While control cases showed no asymmetric uptake between two hemispheres. The abnormal PAC uptake did not respond to external pure sound stimulus, nor did it relate to the seventy of hearing loss assessed by acoustic tests. No anatomical or morphological alteration could be proven on CT/MRI. In conclusion, PET/18F-FDG objectively revealed an increased metabolic change at left PAC in tinnitus, which is of diagnostic value; and there is evidence suggesting tinnitus is most likely induced by a functional change in the brain.

关键词： PET ^18F-FDC 氟18 放射性核素标记正电子发射X线断层摄影术放射性核素显像耳鸣诊断

Phase I study of chimeric antigen receptor modified T cells in treating HER2-positive advanced biliary tract cancers and pancreatic cancers

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Protein & Cell 2018年第10期9卷 838-847页

作者： Kaichao Feng Yang Liu Yelei Guo Jingdan Qiu Zhiqiang Wu Hanren Dai Qingming Yang Yao Wang Weidong Han1,3 Department of Bio-therapeutic Chinese PLA General Hospital Beijing 100853 China Department of Geriatric Hematology Chinese PLA General Hospital Beijing 100853 China Department of Molecular & Immunology Chinese PLA General Hospital Beijing 100853 China

This phase I clinical trial （NCT01935843） is to evaluate the safety, feasibility, and activity of chimeric antigen receptor-engineered T cell （CART） immunotherapy tar- geting human epidermal growth factor receptor 2 （HER2） in patients with advanced biliary tract cancers （BTCs） and pancreatic cancers （PCs）. Eligible patients with HER2-positive （〉50%） BTCs and PCs were enrolled in the trial. Well cultured CART-HER2 cells were infused following the conditioning treatment composed of nab- paclitaxel （100-200 mg/m2） and cyclophosphamide （15-35 mglkg）. CAR transgene copy number in the peripheral blood was serially measured to monitor the expansion and persistence of CART-HER2 cells in vivo. Eleven enrolled patients received 1 to 2-cycle CART- HER2 cell infusion （median CAR＋ T cell 2.1× 10^6/kg）. The conditioning treatment resulted in mild-to-moderate fatigue, nausea/vomiting, myalgialarthralgia, and lym- phopenia. Except one grade-3 acute febrile syndrome and one abnormal elevation of transaminase （〉9 ULN）, adverse events related to the infusion of CART-HER2 cells were mild-to-moderate. Post-infusion toxicities included one case of reversible severe upper gastroin- testinal hemorrhage which occurred in a patient with gastric antrum invaded by metastasis 11 days after the CART-HER2 cell infusion, and 2 cases of grade 1-2delayed fever, accompanied by the release of C-reactive protein and interleukin-6. All patients were evaluable for assessment of clinical response, among which 1 obtained a 4.5-months partial response and 5 achieved stable disease. The median progression free survival was 4.8 months （range, 1.5-8.3 months）. Finally, data from this study demonstrated the safety and feasibility of CART-HER2 immunotherapy, and showed encourag- ing signals of clinical activity.

关键词： HER2 CART biliary tract cancers pancreatic cancers clinical trial

Diagnosis and management of autoimmune pancreatitis:Experience from China

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World Journal of Gastroenterology 2008年第4期14卷 601-606页

作者： Yang Song Quan-Da Liu Ning-Xin Zhou Wen-Zhi Zhang Dian-Jun Wang Emergency Department Chinese PLA GeneralHospital 28^th Fuxing Road Beijing 100853 China Institute of Hepatobiliary Surgery Chinese'PLA General Hospital Beijing100853 China Department of Pathology Chinese PLA GeneralHospital Beijing 100853 China

AIM： To determine the clinical, radiographic and pathologic characteristics, diagnostic and treatment modalities in patients with autoimmune pancreatitis （AIP）. METHODS： In this retrospective study, the data of patients with diagnosed chronic pancreatitis （CP） between 1995 and 2006 in Chinese PLA General Hospital were included to screen for the cases with AIP, according to the following diagnostic criteria：（1） diagnostic histopathologic features, and abound IgG4-positive plasma cells on pancreatic tissues; （2） characteristic imaging on computed tomography and pancreatography, together with increased serum IgG, y-globulin levels or presence of autoantibodies; （3） response to steroid therapy. The clinical, radiographic and pathologic characteristics, diagnostic and treatment modalities, and outcome of AIP cases were reviewed. RESULTS： Twenty-five （22 male, 3 female; mean age 54 years, 36-76 years） out of 510 CP patients were diagnosed as AIP, which accounted for 49% （21/43） of CP population undergoing surgical treatment in the same period. The main clinical manifestations included intermittent or progressive jaundice in 18 cases （72%）, abdominal pain in 11 （44%）, weight loss in 10 （40%）, and 3 cases had no symptoms. The imaging features consisted of pancreatic enlargement, especially in the head of pancreas （18 cases）, strictures of main pancreatic duct and intrapancreatic bile duct. Massive lymphocytes and plasma cells infiltration in pancreatic tissues were showed on pathology, as well as parenchymal fibrosis. Twenty-three patients were misdiagnosed as pancreaticobiliary malignancy, and 21 patients underwent exploratory laparotomy, theremaining 4 patients dramatically responded to steroid therapy. No pancreatic cancer occurred during a mean 46-mo follow-up period. CONCLUSION： AIP patients always are subjected to mistaken diagnosis of pancreatic cancer and an unnecessary surgical exploration, due to its similarity in clinical features with pancreatic cancer. The dif

关键词： Autoimmune pancreatitis Pancreatic neoplasms Differential diagnosis Steroid therapy

Overproduction of nitrate and S-nitrosothiols in diabetic patients

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Journal of Geriatric Cardiology 2008年第1期5卷 25-27页

作者： Junping Zhao Chengbin Wang Hongli Tong Yuzhen Li Chunxi Zhou Yaping Tian Shiwei Liu General Hospital of Chinese PLA Beijing 100853China General Hospital of Chinese PLABeijing 100853China General Hospital of Chinese PLABeijing 100853China General Hospital of Chinese PLABeijing 100853China General Hospital of Chinese PLABeijing 100853China General Hospital of Chinese PLABeijing 100853China General Hospital of Chinese PLABeijing 100853China

Objective The present study was designed to investigate changes in serum or plasma concentrations of nitric oxide and its derivatives in diabetic *** Serum nitrate concentration of 84 diabetic patients was measured by using an enzyme kinetic method,and the plasma S-nitrosothiols concentration of 10 cases was measured by using HPLC *** Serum nitrate concentration and plasma S-nitrosothiols concentration in the diabetics were significantly higher than in control group (P＜0.01 andP＜0.05,respectively).The serum nitrate concentration in diabetics also had a significant positive correlation with the serum glucose concentration (R=0.7256,P＜0.05),but this correlation was not found in control *** These data showed that NO and its derivatives are overproduced in the diabetic patients.(J Geriatr Cardiol 2008;5:25-27)

关键词： S-nitrosothiols nitric oxide diabetics

减少术后尿失禁及尿道狭窄的前列腺癌根治术(附42例报告)

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中华泌尿外科杂志 2001年第11期22卷 681-682页

作者：洪宝发王晓雄肖序仁叶林阳张磊蔡伟李炎唐解放军总医院泌尿外科北京100853

目的　探讨减少前列腺癌根治术后尿道狭窄及尿失禁的术式。　方法　采用膀胱颈粘膜外翻固定再与尿道对端吻合及保留膀胱颈环状肌纤维的前列腺癌根治术治疗前列腺癌 42例。　结果　 42例术后均未发生膀胱尿道吻合口狭窄 ,尿失禁 2例。随... 详细信息

目的　探讨减少前列腺癌根治术后尿道狭窄及尿失禁的术式。　方法　采用膀胱颈粘膜外翻固定再与尿道对端吻合及保留膀胱颈环状肌纤维的前列腺癌根治术治疗前列腺癌 42例。　结果　 42例术后均未发生膀胱尿道吻合口狭窄 ,尿失禁 2例。随诊 4～ 70个月 ,最大尿流率 2 5 .0～30 .7ml/s。　结论　采用膀胱颈粘膜外翻固定再与尿道对端吻合及保留膀胱颈环状肌纤维的前列腺癌根治术 ,术后避免或减少了尿道狭窄 ,减低尿失禁的发生。

关键词：前列腺肿瘤合并症前列腺肿瘤尿失禁尿道狭窄手术疗法

Pancreatic encephalopathy and Wernicke encephalopathy in association with acute pancreatitis: A clinical study

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World Journal of Gastroenterology 2006年第26期12卷 4224-4227页

作者： Guo-Hui Sun Yun-Sheng Yang Qing-Sen Liu Liu-Fang Cheng Xu-Sheng Huang Department of Gastroenterology Chinese PLA General Hospital Beijing 100853 China Department of Neurology Chinese PLA General Hospital Beijing 100853 China

AIM： To investigate clinical characteristics and therapy of pancreatic encephalopathy （PE） and Wernicke encephalopathy （WE）. METHODS： In a retrospective study of 596 patients with acute pancreatitis （AP）, patients with PE were compared to those with WE in regards to history, clinical manifestation, diagnosis, treatment and outcome. RESULTS： There were 93 patients with severe acute pancreatitis （SAP）. Encephalopathies were discovered in 10 patients （1.7%）. Six patients with PE all developed in SAP （6.5%）, and three of them died （3% of SAP, 50% of PE）. Four patients with WE developed in AP （0.7%）, and two of them died （0.3% of AP, 50% of WE）. Two patients with WE were treated with parenteral thiamine and survived. Global confusions were seen in all patients with encephalopathy. Ocular abnormalities were found. Conjugate gaze palsies were seen in 1 of 6 （16.7%） patients with PE. Of 4 patients with WE, one （25%） had conjugate gaze palsies, two （50%） had horizontal nystagmus, three （75%） had diplopia, and one （25%） had myosis. Ataxia was not seen in all patients. None of patients with WE presented with the classic clinical triad. CSF examinations for 2 patients with WE showed lightlyincreased proteins and glucose. CT and MRI of the brain had no evidence of characteristic abnormalities. CONCLUSION： PE occurs in early or reiteration stage of SAP, and WE in restoration stage of SAP/AP. Ocular abnormalities are the hallmarks of WE, and horizontal nystagmus is common. It is difficult to diagnose earlier an encephalopathy as PE or WE, as well as differentiate one from the other. Long fasting, hyperemesis and total enteral nutrition （TPN） without thiamine are main causes of thiamine deficiency in the course of pancreatitis.

关键词： Pancreatic encephalopathy Wernicke encephalopathy Acute pancreatitis Thiamine Total parenteral nutrition