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一期后路全脊椎整块切除术治疗胸椎症状性血管瘤合并脊髓功能障碍

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中华外科杂志 2012年第4期50卷 342-345页

作者：宋若先张永刚张雪松郑国权王岩解放军总医院骨科北京100853

目的探讨一期后路全脊椎整块切除术治疗胸椎症状性血管瘤合并脊髓功能障碍的疗效及其应用价值。方法2006年3月至2010年1月收治胸椎症状性血管瘤伴不同程度脊髓功能障碍患者9例，男性2例，女性7例，年龄14－77岁，平均33．6岁。患者均有... 详细信息

目的探讨一期后路全脊椎整块切除术治疗胸椎症状性血管瘤合并脊髓功能障碍的疗效及其应用价值。方法2006年3月至2010年1月收治胸椎症状性血管瘤伴不同程度脊髓功能障碍患者9例，男性2例，女性7例，年龄14－77岁，平均33．6岁。患者均有不同程度的胸背部疼痛与下肢无力、行走困难。病程3个月至2年，平均14．4个月。患者均为单椎体受累。术前脊髓功能Frankel分级：A级1例，B级3例，C级3例，D级2例，均行一期后路全脊椎（7例）或全椎体（2例）整块切除，同时采用钛网或人工椎体植骨前方重建，联合应用椎弓根螺钉系统内固定。观测术中出血量、术后局部疼痛和脊髓功能恢复情况，以及肿瘤复发、植骨融合、脊柱稳定性情况。结果9例患者均顺利完成手术，手术时间180－270min，平均210min；失血量1000－5000ml，平均1800ml。随访18个月至5年，平均21个月。术前视觉模拟评分平均8．3分，术后3个月平均1．1分，平均下降7．2分；神经症状不同程度恢复或改善，1例患者Frankel分级由术前A级恢复至C级，其余均恢复至E级。末次随访时所有病例无局部复发，植骨融合良好，无钛网移位及脊柱失稳，无内固定钉棒松动断裂迹象。结论一期后路全脊椎整块切除术治疗胸椎症状性血管瘤合并脊髓功能障碍疗效可靠，是一种可显著改善脊髓神经功能，预防肿瘤局部复发，改善患者生存质量的有效术式。

关键词：脊椎肿瘤血管瘤脊髓压迫症胸椎矫形外科手术

A gene encoding an apurinic/apyrimidinic endonuclease-like protein is up-regulated in human gastric cancer

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World Journal of Gastroenterology 2003年第6期9卷 1196-1201页

作者： Gang-ShiWang Meng-WeiWang Ben-YanWu Xiao-BingLiu Wei-You Xin-YanYang DepartmentofGerontalGastroenterology GeneralHospitalofChinesePLABeijing100853China DepartmentofPathology GeneralHospitalofChinesePLABeijing100853China

AIM: To identify the gene that may predispose to human gastric cancer and to analyze its expression in gastric cancer and non-tumorous gastric ***: Cancer, para-tumor, and non-tumor gastric tissues were studied for gene expression profile using fluorescent differential display reverse transcription polymerase chain reaction (DDRT-PCR). The differentially expressed bands of interest were analyzed by cloning,Northern blotting, and sequencing. The sequencing results were compared with the GenBank database for homologyand conserved domain analysis. In situ hybridization with DIG-labeled cRNA probes was used to detect the expression of gene in paraffin embedded gastric adenocarcinoma and non-cancerous ***: A gene expressed higher in tumor and para-tumor tissues than in their non-tumor counterparts of all 7 tested gastric adenocarcinoma patients was identified by means of DDRT-PCR analysis, it was named GCRG213(gastric cancer related gene 213). Northern blot confirmed the differential expression. GCRG213(GenBank No. AY053451) consisted of 1094 base pairs with an open reading frame (ORF) which encoded 142 amino acids. The deduced amino acid sequence contained a putative conserved domain, apurinic/apyrimidinic endonuclease (APE). In situ hybridization analysis showed that C-CRG213was expressed higher in gastric cancer tissues than in their corresponding non-tumor ones. Precancerous leisions of gastric adenocarcinoma showed a high GCRG213 expression, too. No difference of the expression pattems was found between the early and advanced gastric ***: A gene named GCRG213 was identified in human gastric adenocarcinoma. It encoded an APE-like protein which was probably a new member of the APE ***213 was over-expressed not only in gastric cancer,but also in its precancerous leisions. The role of GCRG213 expression in carcinogenesis needs further study.

关键词：胃癌 GCRG213基因基因表达磷酸内切酶肿瘤发生学

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复杂大脑中动脉瘤个性化手术治疗

中华外科杂志 2014年第8期52卷 576-579页

作者：孙正辉武琛王芙昱薛哲许百男周定标解放军总医院神经外科北京100853

目的总结个性化手术治疗大脑中动脉复杂动脉瘤的方法.方法回顾性研究2009年12月至2012年11月解放军总医院神经外科治疗的20例大脑中动脉复杂动脉瘤患者资料.其中男性12例,女性8例;年龄14 - 58岁,平均43岁.6例为巨大动脉瘤（最大径＞2.... 详细信息

目的总结个性化手术治疗大脑中动脉复杂动脉瘤的方法.方法回顾性研究2009年12月至2012年11月解放军总医院神经外科治疗的20例大脑中动脉复杂动脉瘤患者资料.其中男性12例,女性8例;年龄14 - 58岁,平均43岁.6例为巨大动脉瘤（最大径＞2.5 em）,7例为宽颈,3例为梭形,2例有穿支起自瘤颈,6例有重要的分支起自瘤体,2例为介入栓塞后复发的动脉瘤.根据术前影像资料制定个性化手术策略.常规采用额颞入路,术中常规应用体感诱发电位、多普勒血流探测、荧光素血管造影等辅助设备进行监测.术后复查数字减影血管造影（DSA）或CT血管造影（CTA）判断动脉瘤夹闭和架桥血管通畅情况.结果 20例动脉瘤中,7例行多动脉瘤夹组合夹闭,同时载瘤动脉夹闭重建;3例大脑中动脉第一段动脉瘤行动脉瘤近端阻断-颅内外血管高流量架桥术;2例行动脉瘤切除-颞浅动脉-大脑中动脉分支低流量架桥;1例行动脉瘤切除-颞浅动脉-大脑中动脉分支低流量架桥-分支血管侧侧吻合术;2例行动脉瘤切除-远近端吻合术;1例行动脉瘤切除-远近端吻合-豆纹动脉再植术;3例行单侧入路夹闭双侧大脑中动脉瘤;1例行单纯动脉瘤孤立术.出院时格拉斯哥预后评分4-5分19例（良好）,1分1例（术后1周死于心肌梗死）.19例存活患者16例获得随访,随访时间6-39个月,平均20个月,随访期间无再次出血,均复查DSA或CTA,其中14例动脉瘤完全消失,2例有残颈.结论有经验医师采用个性化手术可安全有效地治疗大脑中动脉复杂动脉瘤,血管重建技术是重要的治疗手段.

关键词：大脑中动脉颅内动脉瘤脑血管重建术

Effective and persistent antitumor activity of HER2-directed CAR-T cells against gastric cancer cells in vitro and xenotransplanted tumors in vivo

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Protein & Cell 2018年第10期9卷 867-878页

作者： Yanjing Song Chuan Tong Yao Wang Yunhe Gao Hanren Dai Yelei Guo Xudong Zhao Yi Wang Zizheng Wang Weidong Han Lin Chen Department of General Surgery Chinese PLA General Hospital Beijing 100853 China Medical School of Chinese PLA Beijing 100853 China Bio-therapeutic Department Chinese PLA General Hospital Beijing 100853 China Molecular & Immunology Department Chinese PLA General Hospital Beijing 100853 China

Human epidermal growth factor receptor 2 （HER2） pro- teins are overexpressed in a high proportion of gastric cancer （GC） cases and affect the maintenance of cancer stem cell （CSC） subpopulations, which are used as tar- gets for the clinical treatment of patients with HER2- positive GC. Despite improvements in survival, numer- ous HER2-positive patients fail treatment with trastuzu- mab, highlighting the need for more effective therapies. In this study, we generated a novel type of genetically modified human T cells, expressing a chimeric antigen receptor （CAR）, and targeting the GC cell antigen HER2, which harbors the CD137 and CD3/; moieties. Our findings show that the expanded CART cells, expressing an increased central memory phenotype, were activated by the specific recognition of HER2 antigens in an MHC-in- dependent manner, and effectively killed patient-derived HER2-positive GC cells. In HER2-positive xenograft tumors, CART cells exhibited considerably enhanced tumor inhibition ability, long-term survival, and homing totargets, compared with those of non-transduced T cells. The sphere-forming ability and in vivo tumorigenicity of patient-derived gastric cancer stem-like cells, expressing HER2 and the CD44 protein, were also inhibited. Our results support the future development and clinical application of this adoptive immunotherapy in patients with HER2-positive advanced GC.

关键词： chimeric antigen receptor HER2 gastriccancer cancer stem cell CD137 immunotherapy

The Association between the C5263T Mutation in the Mitochondrial ND2 Gene and Coronary Heart Disease among Young Chinese Han People

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Biomedical and Environmental Sciences 2017年第4期30卷 280-287页

作者： HAN Guo Xin XIA Lei LI Shuo Shuo JIN Qin Hua SONG Yang SHEN Hong WANG Li Li KONG Ling Jie LI Tan Shi ZHU Hai Yan Emergency Department General Hospital of Chinese PLABeijing 100853China Medical Support Department General Hospital of Chinese PLABeijing 100853China Cardiology Department of Chinese PLA Beijing 100853China

Objective This study aimed to investigate the genetic background of mitochondrial genes in young patients with Coronary heart disease （CHD） to provide a foundation for the early prevention of young patients with CHD. Methods 115 cases of young （〈 45 years） CHD Chinese Han patients （case group）, 100 cases of older （〉 45 years） Chinese Hart CHD patients （experimental group） hospitalized and 100 cases of healthy people through physical examination （control group） at the General Hospital of PLA between January 2014 and December 2015 were selected. General information, clinical assessment, pedigree analysis, and mitochondrial full sequence scanning were performed. The pedigrees of one patient harbouring the C5263T mutation were recruited. Mitochondrial functional analysis including cellular reactive oxygen species （ROS） levels and mitochondrial membrane potential （MMP） were performed on pedigrees with the C5263T mutation （mutation group） and without the mutation （non-mutation group）. Results The differences in biochemical tests （P 〉 0.05） between the case group and experimental group were not significant. The C5263T single-nucleotide mutation of the mitochondrial ND2 gene was observed in 2 young CHD patients in the case group. The premature CHD of these 2 patients followed a pattern of maternal inheritance. The mutation group （11, 112） had higher ROS levels （4750.82±1045.55 vs. 3888.58 ± 487.60, P= 0.022） and lower MMP levels （P= 0.045） than the non-mutation group （II1, III1, III2）. Conclusion We speculated that the mitochondrial C5263T mutation might be associated with the occurrence CHD in Chinese Hart young people.

关键词： Mitochondrion Coronary heart disease Young

Inhalation of hydrogen gas reduces liver injury during major hepatotectomy in swine

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World Journal of Gastroenterology 2012年第37期18卷 5197-5204页

作者： Lei Xiang Jing-Wang Tan Li-Jie Huang Lin Jia Ya-Qian Liu Yu-Qiong Zhao Kai Wang Jia-Hong Dong Animal Experiment Centers PLA General Hospital Beijing 100853 China Institute of Hepatobiliary Surgery PLA General Hospital Beijing 100853 China

AIM： To study the effect of H2 gas on liver injury in massive hepatectomy using the Intermittent Pringle maneuver in swine. METHODS： Male Bama pigs （n = 14） treated with ketamine hydrochloride and Sumianxin Ⅱ as induc- tion drugs followed by inhalation anesthesia with 2% isoflurane, underwent 70% hepatotectomy with loss of bleeding less than 50 mL, and with hepatic pedicle occlusion for 20 min, were divided into two groups： Hydrogen-group （n = 7）, the pigs with inhalation of 2% hydrogen by the tracheal intubation during major hepa- totectomy; Contrast-group （n= 7）, underwent 70% hepatotectomy without inhalation of hydrogen. Hemo- dynamic changes and plasma concentrations of alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, hyaluronic acid （HA）, tumor necrosis factor-α （TNF-α）, interleukin-6 （IL-6）, and malondialdehyde （MDA） in liver tissue were measured at pre-operation, post-hepatotectomy （PH） 1 h and 3 h. The apoptosis and proliferating cell nuclear antigen （PCNA） expres- sion in liver remnant were evaluated at PH 3 h. Then we compared the two groups by these marks to evalu- ate the effect of the hydrogen in the liver injury during major hepatotectomy with the Pringle Maneuver in the swine. RESULTS： There were no significant differences in body weight, blood loss and removal liver weight be- tween the two groups. There was no significant differ- ence in changes of portal vein pressure between two groups at pre-operation, PH 30 min, but in hydrogen gas treated-group it slightly decrease and lower than its in Contrast-group at PH 3 h, although there were no significant difference （P = 0.655）. ALT and AST in Hydrogen-group was significantly lower comparing to Contrast-group （P = 0.036, P = 0.011, vs P = 0.032, P = 0.013） at PH 1 h and 3 h, although the two groups all increased. The MDA level increased between the two group at PH i h and 3 h. In the hydrogen gas treated- group, the MDA level was not significantly significant at pre-operation and signif

关键词： Massive hepatotectomy Hydrogen gas, An-ti-oxidant Hyperperfusion Malondialdehyde Oxidativestress

Expression of tissue inhibitor of matrix metalloproteinases-1 during aging in rat liver

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World Journal of Gastroenterology 2005年第24期11卷 3696-3700页

作者： Yu-MeiZhang Xiang-MeiChen DiWu Suo-ZhuShi ZhongYin RuiDing YangLü DepartmentofNephrology GeneralHospitalofPLABeijing100853China

AIM: To investigate the expression and role of tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) during natural aging in rat liver and to detect the expression of matrix metalloproteinase-2 (MMP-2) and MMP-9. METHODS: The rats were divided into 3-mo-old group (n=5), 10-mo-old group (n=5) and 24-mo-old group (n=5). Histopathologic changes of liver were observed with HE and Masson stain. The location and protein expressions of TIMP-1 were determined by immunohistochemistry and Western blot; message RNA (mRNA) levels were measured in livers from rats of various ages by semi-quantitative reverse transcriptional polymerase chain reaction (RT PCR). In addition, the expression of MMP-2 and MMP-9 was assessed by RT-PCR and Western blot. RESULTS: Histologic examination showed that the aging liver had excessive fatty degeneration and collagen deposition. Immunohistochemical staining showed that TIMP-1 related antigen in livers was located in cytoplasm. The protein expression of TTMP-1 was significantly higher in the oldest animals and the mRNA expression was increased significantly in the 24-mo-old rats (t= 4.61, P= 0.002<0.05, 24-vs 10-mo-old rats; t=4.31, P=0.003<0.05, 24- vs 3-mo-old rats). The expression of MMP-2 and MMP-9 had no change during aging; the ratios TIMP-1/MMP-2 and TIMP-1/MMP-9 in aging liver were significantly higher than those in maturation and young livers. CONCLUSION: TIMP-1 may play an important role in the process of liver aging.

关键词： TIMP-1 Aging Rat liver

Transformer-Based Deep Learning Network for Tooth Segmentation on Panoramic Radiographs

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Journal of Systems Science & Complexity 2023年第1期36卷 257-272页

作者： SHENG Chen WANG Lin HUANG Zhenhuan WANG Tian GUO Yalin HOU Wenjie XU Laiqing WANG Jiazhu YAN Xue Medical School of Chinese PLA Beijing 100853China Department of Stomatology the first Medical CentreChinese PLA General HospitalBeijing 100853China Department of Stomatology the First Medical CentreChinese PLA General HospitalBeijing 100853China Beihang University Beijing 100191China

Panoramic radiographs can assist dentist to quickly evaluate patients’overall oral health *** accurate detection and localization of tooth tissue on panoramic radiographs is the first step to identify pathology,and also plays a key role in an automatic diagnosis ***,the evaluation of panoramic radiographs depends on the clinical experience and knowledge of dentist,while the interpretation of panoramic radiographs might lead ***,it is of great significance to use artificial intelligence to segment teeth on panoramic *** this study,SWin-Unet,the transformer-based Ushaped encoder-decoder architecture with skip-connections,is introduced to perform panoramic radiograph *** well evaluate the tooth segmentation performance of SWin-Unet,the PLAGH-BH dataset is introduced for the research *** performance is evaluated by F1 score,mean intersection and Union(IoU)and Acc,Compared with U-Net,Link-Net and FPN baselines,SWin-Unet performs much better in PLAGH-BH tooth segmentation *** results indicate that SWin-Unet is more feasible on panoramic radiograph segmentation,and is valuable for the potential clinical application.

关键词： Deep convolutional neural network panoramic radiograph SWin-Unet Tooth segmentation

Diagnosis of gastric intraepithelial neoplasia by narrow-band imaging and confocal laser endomicroscopy

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World Journal of Gastroenterology 2012年第34期18卷 4771-4780页

作者： Shu-Fang Wang Yun-Sheng Yang Li-Xin Wei Zhong-Sheng Lu Ming-Zhou Guo Jin Huang Li-Hua Peng Gang Sun En-Qiang Ling-Hu Jiang-Yun Meng Departments of Gastroenterology and Hepatology Chinese PLA General Hospital Beijing 100853 China Departments of Pathology Chinese PLA GeneralHospital Beijing 100853 China

AIM:To evaluate the diagnosis of different differentiated gastric intraepithelial neoplasia (IN) by magnifica-tion endoscopy combined with narrow-band imaging (ME-NBI) and confocal laser endomicroscopy (CLE). METHODS:Eligible patients with suspected gastric IN lesions previously diagnosed by endoscopy in secondary hospitals and scheduled for further diagnosis and tratment were recruited for this study. Excluded from the study were patients who had liver cirrhosis, impaired renal function, acute gastrointestinal (GI) bleeding, coagulopathy, esophageal varices, jaundice, and GI post-surgery. Also excluded were those who were pregnant, breastfeeding, were younger than 18 years old, or were unable to provide informed consent. All patients had all mucus and bile cleared from their stom-achs. They then received upper GI endoscopy. When a mucosal lesion is found during observation with whitelight imaging, the lesion is visualized using maximal magnification, employing gradual movement of the tip of the endoscope to bring the image into focus. Saved images are analyzed. Confocal images were evaluated by two endoscopists (Huang J and Li MY), who were familiar with CLE, blinded to the related information about the lesions, and asked to classify each lesion as either a low grade dysplasia (LGD) or high grade dysplasia (HGD) according to given criteria. The results were compared with the final histopathologic diagnosis. ME-NBI images were evaluated by two endoscopists (Lu ZS and Ling-Hu EQ) who were familiar with NBI, blinded to the related information about the lesions and CLE images, and were asked to classify each lesion as a LGD or HGD according to the "microvascular pattern and surface pattern" classification system. The results were compared with the final histopathologic diagnosis. RESULTS: The study included 32 pathology-proven low grade gastric IN and 26 pathology-proven high grade gastric IN that were detected with any of the modalities. CLE and ME-NBI enabled clear v

关键词： Gastric intraepithelial neoplasia Histologicaldiagnosis Confocal laser endomicroscopy Magnifica-tion endoscopy Narrow-band imaging Gastric intraepi-thelial neoplasia lesion