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Coupled strategy based on regulator manipulation and medium optimization empowers the biosynthetic overproduction of lincomycin

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Synthetic and Systems Biotechnology 2024年第1期9卷 134-143页

作者： Xinlu Cai Wanlian Xu Yang Zheng Sendi Wu Rundong Zhao Nian Wang Yaqian Tang Meilan Ke Qianjin Kang Linquan Bai Buchang Zhang Hang Wu school of {1. sciences Institute of Physical Science and Information TechnologyAnhui UniversityHefei230601China State Key Laboratory of Microbial Metabolism Shanghai Jiao Tong UniversityShanghai200240China

The biosynthesis of bioactive secondary metabolites,specifically antibiotics,is of great scientific and economic *** control of antibiotic production typically involves different processes and molecular *** numerous efforts to improve antibiotic yields,joint engineering strategies for combining genetic manipulation with fermentation optimization remain *** A(Lin-A),a lincosamide antibiotic,is industrially fermented by Streptomyces ***,the leucine-responsive regulatory protein(Lrp)-type regulator SLCG_4846 was confirmed to directly inhibit the lincomycin biosynthesis,whereas indirectly controlled the transcription of SLCG_291.,the first reported repressor in *** of SLCG_4846 in the high-yield *** LA21.X(LA21.XΔ4846)increases the Lin-A production and deletion of SLCG_291. in LA21.XΔ4846 exhibits superimposed yield *** the effect of the double deletion on cellular primary metabolism of ***,Plackett-Burman design,steepest ascent and response surface methodologies were utilized and employed to optimize the seed medium of this double mutant in shake flask,and Lin-A yield using optimal seed medium was significantly increased over the *** strategies were performed in a 1.-L *** maximal yield of Lin-A in LA21.XΔ4846-291. reached 6.56 g/L at 21. h,55.1.higher than that in LA21.X at the parental cultivation(4.23 g/L).This study not only showcases the potential of this strategy to boost lincomycin production,but also could empower the development of high-performance actinomycetes for other antibiotics.

关键词： Streptomyces Transcription factor Genetic engineering Fermentation optimization Lincomycin

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Structural engineering of antimicrobials for optimal broad-spectrum activity

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Green Chemical Engineering 2024年第4期5卷 473-482页

作者： Arunmozhiarasi Armugam Diane S.W.Lim Siti Nurhanna Riduan Shook Pui Chan Jerome Asugan Yugen Zhang Institute of Bioengineering and Bioimaging Institute of Sustainability for Chemicals Energy and Environment(ISCE2) Agency for Science Technology and Research (A*STAR) Diploma in Biomedical Science School of Chemical and Life SciencesSingapore Polytechnic

Antimicrobial materials are a crucial component in eradicating and managing the spread of infectious *** are expected to act on a broad-spectrum of microbes, including emerging pathogens which could cause the next Disease X. Herein, we reassessed a series of antimicrobial imidazolium polymers on our shelves and uncovered extended functionality through dual modes of action. By redesigning their structures, a truly broadspectrum antimicrobial material with optimized activity against bacteria(G +ve, G-ve) and fungi, as well as enveloped and non-enveloped viruses was developed. We demonstrated that the imidazolium polymer exhibits dual modes of function against microbes: targeting the microbial membrane and binding DNA. The latter DNA binding affinity was found to be key against non-enveloped viruses. With this insight, we designed small molecule compounds that exhibited optimum broad-spectrum antimicrobial activity and excellent efficacy against ESKAPE group of pathogens that are responsible for some of the deadliest nosocomial infections worldwide. Our results could also shed light on the design of broad-spectrum antimicrobial compounds against Disease X.

关键词： Broad-spectrum Antimicrobial Polyimidazolium Structure-function Membrane active

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Effects of triaxial deformation on the fission barrier in the Z=1.8−1.0 nuclei

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Communications in Theoretical Physics 2024年第6期76卷 92-101页

作者：柴清祯王佳利刘星汉 Institute of Theoretical Physics School of SciencesHenan University of TechnologyZhengzhou 450001China

By using potential energy surface(PES)calculations in the three-dimensional space(β_(2),γ,β_(4))within the framework of the macroscopic-microscopic model,the fission trajectory and fission barrier for Z=1.8(Og),1.9,1.0 nuclei has been systematically *** calculated PES includes macroscopic liquid-drop energy,microscopic shell correction and pairing *** the ^(294)Og1.6 nucleus as an example,we discuss the next closed shell after Z=82 and N=1.6 with the calculated Woods-Saxon single-particle ***,the results of PES in ^(294)Og is illustrated from the(X,Y)scale to the(β_(2),γ)***γdegree of freedom reveals the shape evolution clearly during the fission *** structure near the minimum and saddle point of the PES in the Z=1.8,1.9,1.0 nuclei is demonstrated *** on the potential energy curves,general trends of the evolution of the fission barrier heights and widths are also *** triaxial deformation in these superheavy mass regions plays a vital role in the first fission barrier,showing a significant reduction in both triaxial *** addition,the model-dependent fission barriers of proton-rich nuclei ^(295)Og,2961.9,and 2971.0 are analyzed *** studies could be valuable for synthesizing the superheavy new elements in the forthcoming HIAF and other facilities.

关键词： fission barrier potential energy surface calculation triaxiality Woods-Saxon potential superheavy new elements

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MEAN SENSITIVITY AND BANACH MEAN SENSITIVITY FOR LINEAR OPERATORS

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Acta Mathematica Scientia 2024年第4期44卷 1200-1228页

作者： Quanquan YAO Peiyong ZHU school of Mathematical {1. University of Electronic Science and Technology of ChinaChengdu 611731China

Let(X,T)be a linear dynamical system,where X is a Banach space and T:X→X is a bounded linear *** paper obtains that(X,T)is sensitive(Li-Yorke sensitive,mean sensitive,syndetically mean sensitive,respectively)if and only if(X,T)is Banach mean sensitive(Banach mean Li-Yorke sensitive,thickly multi-mean sensitive,thickly syndetically mean sensitive,respectively).Several examples are provided to distinguish between different notions of mean sensitivity,syndetic mean sensitivi`ty and mean Li-Yorke sensitivity.

关键词： mean sensitive Banach mean sensitive syndetically mean sensitive Li-Yorke sensitive mean Li-Yorke sensitive

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Recent progress of two-dimensional metal-base catalysts in urea oxidation reaction

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Rare Metals 2024年第8期43卷 3607-3633页

作者： Qi-Xiang Huang Fang Wang Yong Liu Bi-Ying Zhang Fang-Ya Guo Zhong-Qiu Jia Hao Wang Tian-Xiang Yang Hai-Tao Wu Feng-Zhang Ren Ting-Feng Yi school of Materials Science and Engineering Henan University of Science and TechnologyLuoyang 471000China school of Environmental Engineering and Chemistry Luoyang Institute of Science and TechnologyLuoyang 471023China Henan Province International Joint Laboratory of Materials for Solar Energy Conversion and Lithium Sodium Based Battery Luoyang 471023China school of Chemistry and {1. sciences Suzhou University of Science and TechnologySuzhou 215009China school of Materials Science and Engineering Northeastern UniversityShenyang110819China

Urea oxidation reaction(UOR)is an auxiliary water electrolysis hydrogen production technology developed in recent years to replace oxygen evolution reaction and reduce energy consumption,which can produce hydrogen more efficiently by low theoretical potential,reduce the average cost of electrochemical hydrogen production,and is a frontier research hotspot for renewable hydrogen ***-dimensional(2D)nanomaterials as electrocatalysts have many favorable potential,such as it can effectively reduce the resistivity of materials and increase the specific surface area with *** paper reviews the application of 2D materials in UOR in alkaline *** a cross-sectional comparison of various material performance data including overpotential,Tafel slope,electrochemical active surface area(ECSA)and it stability test was conducted,which could illustrate the differences between materials composed of different *** addition,the main challenges hindering the progress of research on 2D materials in urea electrocatalysis processes and promising materials in this field in future are summarized and *** is believed that this review will contribute to designing and analyzing highperformance 2D urea electrocatalysts for water splitting.

关键词： Water splitting Urea oxidation reaction Two-dimensional Transition metal compounds Noble/non-noble metals

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METTL9-catalyzed histidine methylation of S1.0A9 suppresses the anti-Staphylococcus aureus activity of neutrophils

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Protein & Cell 2024年第3期15卷 223-229页

作者： Dan Cao Mengyue Lv Chi Hu Shukai Li Siwen Wang Chao Xu Wen Pan Department of Digestive Disease The First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technologyof ChinaHefei230001China Division of life {1. and Medicine The CAS Key Laboratory of Innate Immunity and Chronic DiseaseSchool of Basic Medical SciencesInstitute of ImmunologyUniversity of Science and Technology of ChinaHefei 230027China Division of life {1. and Medicine MOE Key Laboratory for Cellular DynamicsHefei National Center for Cross-Disciplinary SciencesUniversity of Science and Technology of ChinaHefei 230027China

Dear Editor,Protein histidine methylation has been found to be a widespread post-translational modification(PTM)in mammalian cells and represents more than 1.%of all protein methylation events in the human methylome(Ning et al.,201.;Kapell and Jakobsson,2021..Recently,we and other groups independently identified METTL9 as the first broad-specificity histidine N1.methyltransferase in mammals(Davydova et al.,2021.Lv et al.,2021..METTL9 methylates a variety of substrates with a common x-His-x-His motif(His is for histidine;the second x is preferably small amino acids:A/C/G/S)and catalyzes methyl transfer to the last histidine.

关键词： S1.0A9 specificity

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An endophytic fungus interacts with the defensinlike protein OsCAL1.to regulate cadmium allocation in rice

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Molecular Plant 2024年第2期17卷 312-324页

作者： Tianyu Gu Ziai Qi Yating Wang Siying Chen Jing Yan Huapeng Qiu Yanxuan Yu Zjun Fang Junmin Wang Jiming Gong National Key Laboratory of Plant Molecular Genetics Shanghai Center for Plant Stress BiologyCAS Center for Excellence in Molecular Plant SciencesChinese AcademyofSciencesShanghai200032China school of {1. Science and technology ShanghaiTech UniversityShanghai 201210China University of the Chinese Academy of sciences Beijing 100049China Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization College of Life ScienceHunan University of Science and TechnologyXiangtan411201China State Key Laboratory of Crop Stress Adaptation and Improvement School of Life SciencesHenan UniversityKaifeng 475004China Institute of Crops and Nuclear technology Utilization Zhejiang Academy of Agricultural SciencesHangzhou 310021China

Defensin-like proteins are conserved in multicellular organisms and contribute to innate immune re-sponses against fungal *** rice,defensins play a novel role in regulating cadmium(Cd)efflux from the ***,whether the antifungal activity of defensins correlates with Cd-efflux function remains *** this study,we isolated an endophytic Fusarium,designed Fo1.,by a comparative mi-crobiome analysis of rice plants grown in a paddy contaminated with ***1. is tolerant to high levels of Cd,but is sensitive to the defensin-like protein OsCAL1.which mediates Cd efflux to the *** found that Fo1. symbiosis in rice is regulated by OsCAL1.dynamics,and Fo1. coordinates multiple plant pro-cesses,including Cd uptake,vacuolar sequestration,efflux to the environment,and formation of Fe pla-ques in the *** processes are dependent on the salicylic acid signaling pathway to keep Cd levels low in the cytosol of rice cells and to decrease Cd levels in rice grains without any yield ***1. also plays a role in Cd tolerance in the poaceous crop maize and wheat,but has no observed effects in the eudicot plants Arabidopsis and *** together,these findings provide insights into the mech-anistic basis underlying how afungal endophyte and host plant interact to control Cd accumulation in host plants by adapting defense responses to promotethe establishment ofa symbiosis that permits adaptation to high-Cd environments.

关键词： defensin-like protein Fusarium oxysporum endophyte cadmium allocation

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Reduction of unactivated alkyl chlorides enabled by light-induced single electron transfer

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Science China Chemistry 2024年第2期67卷 471-481页

作者： Lei Dai Zhao-Fei Zhang Xiang-Yu Chen school of Chemical {1. University of the Chinese Academy of SciencesBeijing100049China Institute of Chemistry Chinese Academy of SciencesBeijing100190China Binzhou Institute of technology Weiqiao-UCAS Science and Technology ParkBinzhou256606China

Alkyl chlorides are abundant and easily accessible starting ***,due to the high reduction potentials associated with unactivated alkyl chlorides,achieving their single electron reduction remains a persistent *** challenge has spurred the exploration of efficient activation methods to overcome this *** recent years,photocatalysis has emerged as a mild and potent tool for the single electron reduction of unactivated alkyl chlorides,opening up new possibilities in this *** the rapid advancements in this area,a comprehensive review that provides a conceptual understanding of this emerging field,with a specific focus on reaction design and catalytic mechanisms,would be timely and highly ***,we present an overview of various synthetic techniques for photoinduced single electron reduction of unactivated alkyl ***,we also discuss the limitations of the present methods and future directions that lie ahead in this field.

关键词： photocatalysis unactivated alkyl chlorides single electron transfer radical reactions

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Swarm intention identification via dynamic distribution probability image

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Chinese Journal of Aeronautics 2024年第10期37卷 380-392页

作者： Yinhan WANG Jiang WANG Shaoming HE Fei WANG Qi WANG school of Aerospace Engineering Beijing Institute of TechnologyBeijing 100081China Institute of Computing technology Chinese Academy of SciencesBeijing 100190China

In the realm of decision-making for defense and security applications,it is paramount to swiftly and accurately identify the intentions of incoming *** identification methods predominantly focus on single-target applications and overlook the perturbations introduced by measurement *** this study,we propose a novel concept:the Dynamic Distribution Probability(DDP)image,which is constructed using the estimated state and its covariance *** grayscale pixel value within the image signifies the probability of the presence of the agent within the *** proposed identification scheme integrates the use of Extended Kalman Filter(EKF),Convolutional Neural Network(CNN),Back Propagation(BP)network,and Gated Recurrent Unit(GRU)***,the DDP image is processed through a CNN to distill the formation characteristics,and the estimated swarm state from EKF is inputted into a BP network to deduce the kinematic *** outputs from both networks are summed and subsequently channeled into a GRU network to capture temporal *** numerical simulations and flight experiments are presented to demonstrate the robust anti-noise capability of the proposed scheme compared with conventional methods,as well as its superior training efficiency.

关键词： Intention Identification Kalman filters Convolutional neural networks Swarm intelligence

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Identification and functional analysis of CG3526 in spermatogenesis of Drosophila melanogaster

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Insect Science 2024年第1期31卷 79-90页

作者： Qihao Hu Yanhong Xiao Runnan Wei Ting Tang Liang Wen Yuzhen Lu Xiao-Qiang Yu Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied technology Guangzhou Key Laboratory of Insect Development Regulation and Application ResearchInstitute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhou510631 China

Spermatogenesis is a critical part of reproduction in insects;however,its molecular mechanism is still largely *** this study,we identified a testis-specific gene CG3526 in Drosophila *** analysis showed that CG3526 contains a zinc binding domain and 2 C2H2 type zinc fingers,and it is clustered to the vertebrate really interesting new gene(RING)family E3 ubiquitin-protein *** CG3526 was knocked down by RNA interference(RNAi),the testis became much smaller in size,and the apical tip exhibited a sharp and thin end instead of the blunt and round shape in the control *** importantly,compared to the control flies,only a few mature sperm were present in the seminal vesicle of C587-Gal4>CG3526 RNAi *** staining of the testis from CG3526 RNAi flies showed that the homeostasis of testis stem cell niche was disrupted,cell distribution in the apical tip was scattered,and the process of spermatogenesis was not ***,we found that the phenotype of CG3526 RNAi flies’testis was similar to that of testis of Stat92E RNAi flies,the expression level of CG3526 was significantly downregulated in the Stat92EF06346 mutant flies,and the promoter activity of CG3526 was upregulated by *** together,our results indicated that CG3526 is a downstream effector gene in the JAK-STAT signaling pathway that plays a key role in the spermatogenesis of Drosophila.

关键词： CG3526 JAK-STAT spermatogenesis STAT92E testis

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