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Journal of genetics and Genomics 2015年第8期42卷 455-458页

作者： Xiaofeng Jin Rui Fu Wanwan Zhu Zhengxin Liu Tiantian Gu Guanyi Jiao Hua Yang Qi Zhou Zhiqiang Gao Xiao-Yang Zhao department of Otolaryngology Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical College State Key Laboratory of Reproductive Biology Institute of Zoology Chinese Academy of Sciences

In all the connexin-associated human diseases, deafness is one of the most important diseases with high frequency. The mu- tations of GJB2 （gap junction protein β2, also called connexin 26, Cx26） gene link with nonsyndromic or syndromic senso- rineural hearing loss and were shown to account for a large proportion of congenital deaf cases in many studied populations （del Castillo and del Castillo, 201.）. For example, the 235de1. mutation in GJB2 shows the frequency of approximately 1. and is the most frequent mutation in East Asian population （Yan et al., 2003）. Many efforts have been put to study the function of Gjb2 gene in both mouse model and human. In mouse, extensive deletion of Gjb2 causes embryo lethal due to the decreased transplacental glucose uptake, which was not found in human （Takata and Hirano, 1.97; Gabriel et al., 1.98）. In human, GJB2 deficiency is not able to cause embryo lethal （D＇Andrea et al., 2002）. However, the study of GJB2-associated hearing loss is hampered by many difficulties, such as unobtainable human cochlea and acoustic nerve tissues, and therefore the GJB2-associated hearing loss are underlying mechanisms of still remaining unclear.

关键词： GJB In PSCs

Efficiency of microsatellite isolation from orchids via next generation sequencing

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Open Journal of genetics 2012年第4期2卷 167-172页

作者： Madhav Pandey Jyotsna Sharma department of Plant and Soil Science Texas Tech University Lubbock USA

Microsatellites, or simple sequence repeats (SSRs), are highly polymorphic, co-dominant genetic markers commonly used for population genetics analyses although de novo development of species specific microsatellites is cost-and time-intensive. Orchidaceae is one of the most species-rich families of angiosperms with more than 30,000 species estimated. Despite its high species-diversity, microsatellites are available only for a few species and all were developed by only using Sanger sequencing methods. For the first time in orchids, we used 454 GS-FLX sequencing to isolate microsatellites in two species (Cypripedium kentuckiense and Pogonia ophioglossoides), and report preliminary results of the study. From 1.1.th plate that was subjected to sequencing, 32,665 reads were generated, from which 1.,473 fragments contained at least one SSR. We selected 20,697 SSRs representing di-, tri-, and tetra-nucleotides. While 3,674 microsatellites had flanking regions on both sides, useable primer pairs could be designed for 255 SSRs. The mean numbers of reads, SSRs, and SSR-containing reads useful for primer design estimated for other 1. orchid species using Sanger sequencing method were 1.6, 78 and 31. respectively. Results demonstrate that the efficiency of microsatellite isolation in orchids is substantially higher with 454 GS-FLX sequencing technique in comparison to the Sanger sequencing methods.

关键词： 454 GS-FLX Sequencing Cypripedium kentuckiense Microsatellites Orchids Pogonia ophioglossoides Sanger Sequencing

Generation of rat blood vasculature and hematopoietic cells in rat-mouse chimeras by blastocyst complementation

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Journal of genetics and Genomics 2020年第5期47卷 249-261页

作者： Xiaomin Wang Hui Shi Juanjuan Zhou Qingjian Zou Quanjun Zhang Shixue Gou Pengfei Chen Lisha Mou Nana Fan Yangyang Suo Zhen Ouyang Chengdan Lai Quanmei Yan Liangxue Lai CAS Key Laboratory of Regenerative Biology Guangdong Provincial Key Laboratory of Stem Cell and Regenerative MedicineSouth China Institute for Stem Cell Biology and Regenerative MedicineGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou510530China University of Chinese Academy of Sciences Beijing100049China Guangzhou Regenerative Medicine and Health Guangdong Laboratory(GRMH-GDL) Guangzhou510005China Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijing100101China Research Unit of Generation of Large animal Disease Models Chinese Academy of Medical Sciences(2019RU015)Guangzhou510530China School of Biotechnology and Health Sciences Wuyi UniversityJiangmen529020China Shenzhen Xenotransplantation Medical Engineering Research and Development Center Institute of Translational MedicineShenzhen University Health Science CenterShenzhen University School of MedicineFirst Affiliated Hospital of Shenzhen UniversityShenzhen Second People's HospitalShenzhen518035China department of Central Laboratory Shenzhen Longhua District Central HospitalShenzhen518110China School of Life Sciences University of Science and Technology of ChinaHefei230027China Joint School of Life Sciences Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou Medical UniversityGuangzhou511436China

Interspecies chimera through blastocyst complementation could be an alternative approach to create human organs in animals by using human pluripotent stem cells.A mismatch of the major histocompatibility complex of vascular endothelial cells between the human and host animal will cause graft rejection in the transplanted ***,to achieve a transplantable organ in animals without rejection,creation of vascular endothelial cells derived from humans within the organ is *** this study,to explore whether donor xeno-pluripotent stem cells can compensate for blood vasculature in host animals,we generated rat-mouse chimeras by injection of rat embryonic stem cells(rESCs)into mouse blastocysts with deficiency of Flk-1.protein,which is associated with endothelial and hematopoietic cell *** found that rESCs could differentiate into vascular endothelial and hematopoietic cells in the rat-mouse *** whole yolk sac(YS)of Flk-1.EGFP/ECFP rat-mouse chimera was full of rat blood *** genes related to vascular endothelial cells,arteries,and veins,blood vessels formation process,as well as hematopoietic cells,were highly expressed in the *** results suggested that rat vascular endothelial cells could undergo proliferation,migration,and self-assembly to form blood vasculature and that hematopoietic cells could differentiate into B cells,T cells,and myeloid cells in rat-mouse chimeras,which was able to rescue early embryonic lethality caused by Flk-1.deficiency in mouse.

关键词： Blastocyst complementation Interspecies chimera Intraspecies chimera Flk-1 Vascular endothelial cell Hematopoietic cell

Deciphering Starch Quality of Rice Kernels Using Metabolite Profiling and Pedigree Network Analysis

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Molecular Plant 2012年第2期5卷 442-451页

作者： Miyako Kusano Atsushi Fukushima Naoko Fujita Yozo Okazaki Makoto Kobayashi Naoko Fujita Oitome Kaworu Ebana Kazuki Saito RIKEN Plant Science Center Tsurumi Yokohama 230-0045 Japan KIHARA Institute for Biological Research Yokohama City University Totsuka Yokohama 244-0813 Japan department of Biological Production Akita Prefectural University Akita 010-0195 Japan National Institute of Agrobiological Sciences 2-1-2 Kannondai Tsukuba Ibaraki 305-8602 Japan Graduate School of Pharmaceutical Sciences Chiba University Chiba 260-8675 Japan

The physiological properties of rice grains are immediately obvious to consumers. High-coverage metabolomic characterization of the rice diversity research set predicted a negative correlation between fatty acid and lipid levels and amylose/total starch ratio （amylose ratio）, but the reason for this is unclear. To obtain new insight into the relationships among the visual phenotypes of rice kernels, starch granule structures, amylose ratios, and metabolite changes, we investigated the metabolite changes of five Japonica cultivars with various amylose ratios and two knockout mutants （e 1. a Starch synthase Ilia （SSIIla）-deficient mutant and the SSIIla/starch branching enzyme （BE） double-knockout mutant 401.） by using mass spectrometry-based metabolomics techniques. Scanning electron microscopy clearly showed that the two mutants had unusual starch granule structures. The metabolomic compositions of two cultivars with high amylose ratios （Hoshiyutaka and Yumetoiro） exhibited similar patterns, while that of the double-knockout mutant, which has an extremely high amylose ratio, differed. Rice pedigree network analysis of the cultivars and the mutants provided insight into the association between metabolic-trait properties and their underlying genetic basis in rice breeding in Japan. Multidimensional scaling analysis revealed that the Hoshiyutaka and Yumetoiro cultivars were Indica-like, yet they are classified as Japonica subpopulations. Exploring metabolomic traits is a powerful way to follow rice genetic traces and breeding history.

关键词： starch quality traits amylose ratio metabolite profiling pedigree networks rice.

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湿度对田间自然热罩防治柑橘黄龙病的影响

福建农业学报 2014年第5期29卷 475-482页

作者：林雄杰范国成胡菡青王贤达 Yulu Xia 蔡子坚刘波福建省农业科学院果树研究所福建福州350013 The United States department of Agriculture Animal and Plant Health Inspection ServicePlant Protection and QuarantineCenter for Plant Health Science and Technology 福建省农业科学院福建福州350003

比较加湿处理后热罩和自然条件下热罩对黄龙病的防治效果,结果表明自然条件处理后30d叶片平均病菌浓度下降了85%以上,而加湿条件下叶片平均病菌浓度仅下降3.7%,与自然条件处理间差异达到显著水平;自然条件热罩处理后90d叶片平均病菌浓... 详细信息

比较加湿处理后热罩和自然条件下热罩对黄龙病的防治效果,结果表明自然条件处理后30d叶片平均病菌浓度下降了85%以上,而加湿条件下叶片平均病菌浓度仅下降3.7%,与自然条件处理间差异达到显著水平;自然条件热罩处理后90d叶片平均病菌浓度下降了97%以上,而加湿处理病树叶片平均病菌浓度下降了38%,与自然条件处理间的差异达到极显著水平。对照组叶片平均病菌浓度处理后30d和90d均有略微增加;加湿未热罩处理叶片平均病菌浓度在处理后30d和90d分别增加了4.5倍和8.7倍,但与对照组无显著差异。本研究结果表明加湿热罩处理病树显著减弱了其杀灭黄龙病菌效果,同时表明增加土壤湿度对黄龙病菌繁殖可能具有一定的促进作用。

关键词：柑橘黄龙病湿度自然热罩实时荧光定量PCR 影响

Development of Chromosome Segment Substitution Lines and Genetic Dissection of Grain Size Related Locus in Rice

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Rice science 2021年第4期28卷 322-324,I0012-I0017页

作者： Aamir Riaz Wang Huimin Zhang Zhenhua Workie Anley Zegeye Li Yanhui Wang Hong Xue Pao Peng Zequn Shen Xi hong Cheng Shihua Zhang Yingxin State Key Laboratory of Rice Biology/National Center for Rice Improvement China National Rice Research InstituteHangzhou 310006China department of Plant Science University of GondarGondar 196Ethiopia

To investigate the genetic bases of grain weight(GW),a set of 76 chromosome segment substitution lines(CSSLs,BC4F5)were developed from the cross between Xieqingzao B(XQZB)and Zhonghui 9308(ZH9308)using a marker-assisted selection(MAS).All substituted segments represented by 1.0 markers in those lines covered 96.7%of the donor parent(ZH9308).Consequently,two QTLs(qTGW1.and qTGW6)for 1.00-grain weight(TGW)were preliminarily mapped on chromosomes 1.and 6,*** line CSSL7 was selected for further mapping of qGL1.4/*** a result,qGL1.4 was validated and narrowed to a 1.4 Mb interval between markers InDel1. and RM1.872 using secondary F2,and narrowed to a 500 kb in F2:3 populations between markers RM1.824 and *** F4:5 secondary population,using homozygous recombinant strategy,qGL1.4 was finally fine-mapped to a 286.4 kb region between markers D-1. and *** addition,a near-isogenic line(NIL)harbouring qGL1.4 was developed using MAS approach,which showed enhanced grain length compared with ZH9308 without changing other *** summary,these results lay a foundation for the genetic isolation of qGL1.4 and molecular breeding in rice.

关键词： breeding narrow bases

Overview of purple blotch disease and understanding its management through chemical, biological and genetic approaches

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Journal of Integrative Agriculture 2020年第12期19卷 3013-3024页

作者： Aejaz Ahmad DAR Susheel SHARMA Reetika MAHAJAN Muntazir MUSHTAQ Ankila SALATHIA Shahid AHAMAD Jag Paul SHARMA School of Biotechnology Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu(SKUAST-J)Jammu 180009India department of Research Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu(SKUAST-J)Jammu 180009India

Purple blotch disease of Allium spp. crops caused by Alternaria porri has remained a major concern in agriculture for both farmers and research fraternity as it severely damages the crops and drastically reduces the yield. The symptoms appear after 1.4 days of infection and bulb rot begin, and eventually turn into dark reddish-purple and then brownish/black lesions. Many factors like season, time of sowing, humidity and temperature, stage of crop, and plant architecture have a huge impact on the progression of purple blotch disease. Many genic markers based on amplification of an Alta1.gene sequence have been designed for identification and differentiation of different Alternaria species groups. Among the most commonly used fungicides, mancozeb, tebuconazole, difenaconazole and azoxystrobin were found to be the ideal for the management of purple blotch disease and increased garlic yield. Many biological approaches such as plant extracts and bio-control agents were found partially effective for controlling the disease. A report on QTL mapping for purple blotch resistance discovered that purple blotch resistance is controlled by a single dominant gene ApR1. To completely understand the purple blotch disease resistance for crop improvement, a study is required at transcriptome level for hunting purple blotch resistant genes by gene annotation and mining. Genetic engineering and genome editing are other approaches that can be done for engineering disease resistance in Allium crops for genetic improvement.

关键词： purple blotch Alternaria porri breeding QTL mapping genome editing genetic engineering transcriptome analysis

Factors Associated with Type 1.and Type 2 Diabetes in Infants in Kuwait

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Health 2018年第11期10卷 1474-1486页

作者： Nedaa A. Al-Khamees Curriculum and Instruction department College of Education Kuwait University Audaylia Kuwait

Purpose: The purpose of the study is to investigate the factors associated with diabetes and its influence on the prevalence of diabetes in Kuwait. Methods: A questionnaire was designed to explore the relationships between various factors and diabetes in children. The survey included 300 mothers of children up to five years of age in Kuwait and the results were subjected to a two-way contingency table analysis. Results: Variables showing at least a medium effect and a linear relationship are herein reported. Increasing maternal age was found to be related to the increasing prevalence of type 1.diabetes. An association between a higher prevalence of both type 1.and type 2 diabetes in the mother and the child was found. Risk of having a child with diabetes increases with the maternal age. The consumption of fried food or fast food by a child was associated with a higher prevalence of both type 1.and type 2 diabetes. Sugar consumption, both by the mother during pregnancy and by the child, was associated with a higher prevalence of type 2 diabetes. Heavy consumption of nuts by the child was associated with a higher prevalence of type 2. Conclusion: genetics and environmental factors including diet may play important roles in the causation of both type 1.and type 2 diabetes in Kuwait.

关键词： Type 1 Diabetes Type 2 Diabetes Children Kuwait

Radiation-Induced Lung Cancers in Murine Models

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Advances in Lung Cancer 2014年第2期3卷 38-44页

作者： Leena Rivina Michael Davoren Robert H. Schiestl department of Environmental Health Sciences University of California Los Angeles USA

Radiation therapy is a key weapon in the modern arsenal of cancer treatment. However, this effective treatment comes with risks of its own, and the sheer number of patients that undergo radiation as a part of their therapy regimen is only increasing. As this number increases, so does the incidence of secondary, radiation-induced neoplasias, creating a need for therapeutic agents targeted specifically towards reduction in the incidence of and treatment of these cancers. Development and efficacy testing of these agents requires not only extensive?in vitro?testing, but also a set of reliable animal models to accurately recreate the complex situations of radiation-induced carcinogenesis. The laboratory mouse?Mus musculus?remains the most relevant animal model in cancer research due to the molecular and physiological similarities it shares with man, its small size and high rate of breeding in captivity, and its fully sequenced genome. In this work, we review relevant?M. musculusinbred and?F1.hybrid animal models, as well as methods of induction of radiation-induced lung cancers. Associated molecular pathologies are also included.

关键词： Radiation Carcinogenesis animal Models Radiation Protectors Radiation Mitigators Secondary Cancers

Fungal diversity notes 1.1.252-taxonomic and phylogenetic contributions to fungal taxa

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Fungal Diversity 2015年第6期75卷 27-274页

作者： Hiran A.Ariyawansa Kevin D.Hyde Subashini C.Jayasiri Bart Buyck K.W.Thilini Chethana Dong Qin Dai Yu Cheng Dai Dinushani A.Daranagama Ruvishika S.Jayawardena Robert Lücking Masoomeh Ghobad-Nejhad Tuula Niskanen Kasun M.Thambugala Kerstin Voigt Rui Lin Zhao Guo-Jie Li Mingkwan Doilom Saranyaphat Boonmee Zhu L.Yang Qing Cai Yang-Yang Cui Ali H.Bahkali Jie Chen Bao Kai Cui Jia Jia Chen Monika C.Dayarathne Asha J.Dissanayake Anusha H.Ekanayaka Akira Hashimoto Sinang Hongsanan E.B.Gareth Jones Ellen Larsson Wen Jing Li Qi-Rui Li Jian Kui Liu Zong Long Luo Sajeewa S.N.Maharachchikumbura Ausana Mapook Eric H.C.McKenzie Chada Norphanphoun Sirinapa Konta Ka Lai Pang Rekhani H.Perera Rungtiwa Phookamsak Chayanard Phukhamsakda Umpava Pinruan Emile Randrianjohany Chonticha Singtripop Kazuaki Tanaka Cheng Ming Tian Saowaluck Tibpromma Mohamed A.Abdel-Wahab Dhanushka N.Wanasinghe Nalin N.Wijayawardene Jin-Feng Zhang Huang Zhang Faten A.Abdel-Aziz Mats Wedin Martin Westberg Joseph F.Ammirati Timur S.Bulgakov Diogo X.Lima Tony M.Callaghan Philipp Callac Cheng-Hao Chang Luis F.Coca Manuela Dal-Forno Veronika Dollhofer Kateřina Fliegerová Katrin Greiner Gareth W.Griffith Hsiao-Man Ho Valerie Hofstetter Rajesh Jeewon Ji Chuan Kang Ting-Chi Wen Paul M.Kirk Ilkka Kytövuori James D.Lawrey Jia Xing Hong Li Zou Yi Liu Xing Zhong Liu Kare Liimatainen H.Thorsten Lumbsch Misato Matsumura Bibiana Moncada Salilaporn Nuankaew Sittiporn Parnmen AndréL.C.M.de Azevedo Santiago Sujinda Sommai Yu Song Carlos A.F.de Souza Cristina M.de Souza-Motta Hong Yan Su Satinee Suetrong Yong Wang Syuan-Fong Wei Ting Chi Wen Hai Sheng Yuan Li Wei Zhou Martina Réblová Jacques Fournier Erio Camporesi J.Jennifer Luangsa-ard Kanoksri Tasanathai Artit Khonsanit Donnaya Thanakitpipattana Sayanh Somrithipol Paul Diederich Ana M.Millanes Ralph S.Common Marc Stadler Ji Ye Yan XingHong Li Hye Won Lee Thi T.T.Nguyen Hyang Burm Lee Eliseo Battistin Orlando Marsico Alfredo Vizzini Jordi Vila Enrico Ercole Ursula Eberhardt Giampaolo Simonini Hua-An Wen Xin-Hua Chen Otto Mie Guizhou Key Laboratory of Agricultural Biotechnology Guizhou Academy of Agricultural SciencesGuiyang 550006GuizhouChina Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of ScienceKunming 650201YunnanChina Centre of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailan Département Systématique et Evolution CP 39ISYEBUMR 7205 CNRS MNHN UPMC EPHEMuséum National d’Histoire Naturelle12 Rue BuffonF-75005 ParisFrance Institute of Plant and Environment Protection Beijing Academy of Agriculture and Forestry SciencesNo.9 of Shu Guang Hua Yuan Zhong LuHaidian DistrictBeijing 10097China Microbial Drugs Helmholtz-Centre for Infection Research Inhoffenstrasse 738124 BraunschweigGermany Staatliches Museum für Naturkunde Stuttgart Rosenstein 1D-70191StuttgartGermany Institute of Microbiology Beijing Forestry UniversityPO Box 61Beijing 100083China State Key Laboratory of Mycology Institute of MicrobiologyChinese Academy of SciencesNo 31st West Beichen RoadChaoyang DistrictBeijing 100101China The Herbarium(ANDA) Universitas AndalasPadangSumatera Barat 25163Indonesia Botanischer Garten&Botanisches Museum Berlin Freie Universität BerlinKönigin-Luise-Straße 6-814195 BerlinGermany department of Biotechnology Iranian Research Organization for Science and Technology(IROST)P.O.Box 3353-5111Tehran ***Iran Jodrell Laboratory Royal Botanic GardensKew TW93DSUK Leibniz Institute for Natural Product Research and Infection Biology Jena Microbial Resource CollectionAdolf-Reichwein-Strasse 2307745 JenaGermany department of Botany and Microbiology College of ScienceKing Saud UniversityP.O.Box:2455Riyadh 1145Saudi Arabia The United Graduate School of Agricultural Sciences Iwate University18-8 Ueda 3 ChomeMorioka 020-8550Japan Faculty of Agriculture and Life Sciences Hirosaki University3 Bunkyo-choHirosakiAomori 036-8561Japan department of Biological and Environmental Sciences University of GothenburgBox 461SE

This paper is a compilation of notes on 1.2 fungal taxa,including five new families,20 new genera,and 1.0 new species,representing a wide taxonomic and geographic *** new families,Ascocylindricaceae,Caryosporaceae and Wicklowiaceae(Ascomycota)are introduced based on their distinct lineages and unique *** new Dothideomycete genera Pseudomassariosphaeria(Amniculicolaceae),Heracleicola,Neodidymella and Pseudomicrosphaeriopsis(Didymellaceae),Pseudopithomyces(Didymosphaeriaceae),Brunneoclavispora,Neolophiostoma and Sulcosporium(Halotthiaceae),Lophiohelichrysum(Lophiostomataceae),Galliicola,Populocrescentia and Vagicola(Phaeosphaeriaceae),Ascocylindrica(Ascocylindricaceae),Elongatopedicellata(Roussoellaceae),Pseudoasteromassaria(Latoruaceae)and Pseudomonodictys(Macrodiplodiopsidaceae)are *** newly described species of Dothideomycetes(Ascomycota)are Pseudomassariosphaeria bromicola(Amniculicolaceae),Flammeascoma lignicola(Anteagloniaceae),Ascocylindrica marina(Ascocylindricaceae),Lembosia xyliae(Asterinaceae),Diplodia crataegicola and Diplodia galiicola(Botryosphaeriaceae),Caryospora aquatica(Caryosporaceae),Heracleicola premilcurensis and Neodidymella thailandicum(Didymellaceae),Pseudopithomyces palmicola(Didymosphaeriaceae),Floricola viticola(Floricolaceae),Brunneoclavispora bambusae,Neolophiostoma pigmentatum and Sulcosporium thailandica(Halotthiaceae),Pseudoasteromassaria fagi(Latoruaceae),Keissleriella dactylidicola(Lentitheciaceae),Lophiohelichrysum helichrysi(Lophiostomataceae),Aquasubmersa japonica(Lophiotremataceae),Pseudomonodictys tectonae(Macrodiplodiopsidaceae),Microthyrium buxicola and Tumidispora shoreae(Microthyriaceae),Alloleptosphaeria clematidis,Allophaeosphaeria cytisi,Allophaeosphaeria subcylindrospora,Dematiopleospora luzulae,Entodesmium artemisiae,Galiicola pseudophaeosphaeria,Loratospora luzulae,Nodulosphaeria senecionis,Ophiosphaerella aquaticus,Populocrescentia forlicesenensis and Vagicola vagans(Phaeosphaeriaceae),Elon

关键词： Fungi Taxonomy New genus New species Phylogeny