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Preparation and Targeting Evaluation of Recombined Intrakine Plasmid pEGFP/SDF-1.KDE/Folate-Liposome Complexes

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Chinese Journal of biomedical {1.(English Edition) 2010年第1期19卷 35-40页

作者： CHEN Hong-yuan RUI Wen XU Jun CAI Shao-hui Department of Microbiology and Immunology Guangdong Pharmaceutical University Guangzhou 510006 China Laboratory center of Guangdong Pharmaceutical University Guangzhou 510006 China Pharmacology Laboratory center Jinan University Guangzhou 510632 China

Objective:To prepare folate liposome complexes of recombinant plasmid pEGFP/SDF-1.KDEL wich contained intrakine SDF-1.KDEL gene and to observe its targeting for breast cancer ***:Reverse phase evaporation method was employed for preparing folate-liposome complexeses of pEGFP/SDF-1.KDEL,so that preparation condition was optimized;and fluorometric method was taken for testing encapsulation *** diameter of liposome was tested by transmission electron *** encapsulated with 0.05-0.25 mg/ml calcein were got and incubated with breast cancer cell line MDA-MB-231.cells for 2 to 1. h,then dissolved them with dimethyl sulfoxide,then detected absorbance with microplate ***:The optimized encapsulation condition are as follows:molecular ratio of lecithin and cholesterol was 3:1.rotary speed was 1.0 r/min under temperature of 43℃,and encapsulation efficiency reached 81.in this *** liposome particle diameter was 21. nm;8 h after incubation, the intake of MDA-MB-231.cells to 0.25 mg/ml compounds achieved saturation. Conclusion:The pEGFP/SDF-1.KDEL folic acid liposome complexes prepared has a high encapsulating rate,liposome particle diameters are homogeneous,which is available for targeting for breast cancer cells in vitro.

关键词： intrakine folate liposome targeting

SAXS and SANS Investigation of Synthetic Cholesteric Liquid-Crystal Polymers for biomedical Applications

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材料科学与工程（中英文B版） 2013年第2期3卷 104-116页

作者： Mercedes Perez Mendez Boualem Hammouda Institute of Polymer Science and Technology CSIC Juan de la Cierva Madrid 3 28006 Spain NIST center for Neutron Research National Institute of Standards and Technology Gaithersburg 20899-6102 MD USA

Multifunctional cholesteric liquid-crystal polymer, designated [PTOBEE]1.-NH2, has been synthesized, from precursor PTOBEE [C26H20O8]n previously reported, by functionalization with amine groups. Cholesteric PTOBEE has been shown to be able to entrap DNA, being tested as non-viral vector in gene therapy, including cationic monomeric surfactants in their formulation. The direct interaction between cationic cholesteric liquid-crystal polymer PTOBEE-NH2, with anionic commercial [Poly-C-Poly-G], is proposed as new formulation for biomedical applications. The interaction mechanism is studied in three different volume ratios: (1.2), (1.1. and (2:1., respectively. Their structures, studied by SAXS at ESRF, provide information about the complexes size and shape. Based on preliminary neutron scattering experiments, showing sufficient contrast (scattering length density difference) between cholesteric PTOBEE-NH2 (1.5 to1.9 ? 1.1. /cm2) and polynucleotide [PolyC-PolyG] (3.32 ? 1.1./cm2), contrast variation SANS experiments were performed at NIST, with a wavelength of λ = 8A, using different H2O: D2O mixtures (i. e., contrasts) to match the cholesteric polymer (44.5%:55.5%), the polynucleotide (65% or 70%):(35% or 30%) or the whole complex (30%:70%), being able to "observe separately" both component structures within the complexes. SANS results agree and complement the information obtained by SAXS. Different TWO SHELL molecular models are proposed for the interaction by using a combination of model-fitting (FISH).

关键词：液晶聚合物生物医学应用 SANS 小角X射线散射胆甾型合成阳离子单体相互作用

活化蛋白C通过内皮细胞蛋白C受体抑制TNF-α介导的炎症反应(英文)

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生物医学工程学杂志 2009年第3期26卷 625-630页

作者：陈友琴彭军刘肖珩吴江李汝恒郑晓红 Department of Pediatrics University of Oklahoma Health Sciences Center 940 Stanton L. Young Boulevord Oklahoma City OK 73190 USA Department of Biological Sciences National University of Singapore 14 Science Drive 4 Singapore 117543 福建中医学院中西医结合研究院福州350108 四川大学华西医学中心生物医学工程研究室成都610041 大理学院物理与电子信息学院大理671003

活化蛋白C(APC)在抑制炎症反应中起着重要作用,内皮细胞蛋白C受体(EPCR)可以增加蛋白C的活化。已有的研究表明:APC通过抑制细胞因子和化学趋化因子的分泌以及黏附分子的表达来调节炎症反应,但其作用机制还不完全明了。本文研究了APC对T... 详细信息

活化蛋白C(APC)在抑制炎症反应中起着重要作用,内皮细胞蛋白C受体(EPCR)可以增加蛋白C的活化。已有的研究表明:APC通过抑制细胞因子和化学趋化因子的分泌以及黏附分子的表达来调节炎症反应,但其作用机制还不完全明了。本文研究了APC对TNF-α诱导的细胞因子分泌和黏附分子表达的影响,同时探讨了其可能的作用机制。结果显示:APC能显著抑制TNF-α诱导的细胞因子分泌(IL-1.和IL-8)和黏附分子表达(ICAM-1.VCAM-1.and E-selction);抗EPCR的单克隆抗体显著拮抗了APC抑制细胞因子分泌和黏附分子表达的功能。结论:APC通过抑制炎症因子的产生来调节炎症反应,这种作用可能是通过与内皮细胞EPCR的相互作用来实现。

关键词：活化蛋白C 内皮细胞蛋白C受体细胞因子黏附分子

吲哚-3-甲醇酸性代谢产物辐射防护作用的研究

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国际生物医学工程杂志 2016年第3期39卷 144-148页

作者：储小飞赵舒怡崔明路璐张俊玲孟庆慧樊赛军中国医学科学院北京协和医学院放射医学研究所天津市放射医学与分子核医学重点实验室天津300192 Washington DC Department of Oncology Lombardi Comprehensive Cancer Center Georgetown University20097

目的探讨吲哚-3-甲醇（1.C）酸性代谢产物的辐射损伤防护作用。方法采用细胞克隆形成实验检测细胞的存活分数；采用WesternBlot方法检测蛋白表达水平。结果采用正常纤维上皮细胞1.4A1.发现7种1.C酸性代谢产物表现出不同的辐射损伤防护... 详细信息

目的探讨吲哚-3-甲醇（1.C）酸性代谢产物的辐射损伤防护作用。方法采用细胞克隆形成实验检测细胞的存活分数；采用WesternBlot方法检测蛋白表达水平。结果采用正常纤维上皮细胞1.4A1.发现7种1.C酸性代谢产物表现出不同的辐射损伤防护效果，其中CTET（1.mol／L）、LTET（1.mol/L）、HI-IM（1.mol／L）和3，3＇-二吲哚甲烷（DIM）（0-3μmol／L）4种代谢产物在细胞γ-射线照射前24h预处理细胞均不同程度地提高了辐照细胞的存活分数，差异均有统计学意义（P〈0．05，P〈0．01.，而CT（1.mol／L）、ITr-1.1.mol／L）和ICZ（1.mo／／L）3种代谢产物则对辐照细胞存活分数无影响（P≥0．05）。进一步研究发现CTET、LTET、HI—IM和DIM可导致ATM、BRCA1.NBS1.白磷酸化水平的改变。同样，CT、LTr-1.ICZ对这些蛋白的磷酸化水平不产生影响。另外，HI-IM可明显降低辐射损伤诱导的细胞坏死和细胞凋亡。结论CTET、LTET、HI-IM和DIM4种1.C酸性代谢产物可明显降低1.4A1.胞的辐射敏感性，即具有辐射防护作用，其机制可能与其调节DNA损伤与修复蛋白磷酸化和细胞凋亡有关。

关键词：吲哚-3-甲醇酸性代谢产物辐射损伤 DNA损伤修复细胞凋亡

唇腭裂跨膜蛋白1.蛋白对肺癌细胞放射敏感性影响的研究

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国际生物医学工程杂志 2016年第3期39卷 149-152,162页

作者：赵舒怡储小飞刘伟利孟庆慧崔明樊赛军中国医学科学院北京协和医学院放射医学研究所天津市放射医学与分子核医学重点实验室天津300192 Washington DC Depart-ment of Oncology Lombardi Comprehensive Cancer Center Georgetown University20097

目的对唇腭裂跨膜蛋白1.蛋白（CLPTM1.）的表达与肺癌细胞放射敏感性改变的相关性进行研究。方法采用噻唑蓝（M1.r）方法和细胞克隆形成实验检测细胞的生长和生存率；采用蛋白质印迹（WesternBlot）方法检测蛋白表达水平。结果不同肺... 详细信息

目的对唇腭裂跨膜蛋白1.蛋白（CLPTM1.）的表达与肺癌细胞放射敏感性改变的相关性进行研究。方法采用噻唑蓝（M1.r）方法和细胞克隆形成实验检测细胞的生长和生存率；采用蛋白质印迹（WesternBlot）方法检测蛋白表达水平。结果不同肺癌细胞的放射敏感性与其细胞中CLPTM1.的蛋白表达水平呈负相关，CLPTM1.高表达的肺癌细胞表现出低放射敏感性；提高肺癌细胞中CLPTM1.的表达水平可明显降低γ射线辐照后细胞的放射敏感性，相反转染CLPTM1.小干扰RNA（siRNA）降低CLPTM1.表达水平则大大提高了肺癌细胞的放射治疗敏感性。结论CLPTM1.可能是调节肺癌放射治疗敏感性的一种新靶向基因。

关键词：唇腭裂跨膜蛋白1.蛋白辐射耐受性肺肿瘤 γ射线

Differential regulation of JAK1.expression by ETS1.associated with predisposition to primary biliary cholangitis

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Journal of Genetics and Genomics 2023年第10期50卷 807-812页

作者： Peng Jiang Chan Wang Mingming Zhang Ye Tian Weifeng Zhao Junyi Xin Yexi Huang Zhibin Zhao Wenjuan Sun Jie Long Ruqi Tang Fang Qiu Xingjuan Shi Yi Zhao Li Zhu Na Dai Lei Liu Xudong Wu Jinshan Nie Bo Jiang Youlin Shao Yueqiu Gao Jianjiang Yu Zhigang Hu Zhidong Zang Yuhua Gong Yaping Dai Lan Wang Ningling Ding Ping Xu Sufang Chen Lu Wang Jing Xu Luyao Zhang Junyan Hong Ruonan Qian Hu Li Xuan Jiang Congwei Chen Wenyan Tian Jian Wu Yuzhang Jiang Chongxu Han Kui Zhang Hong Qiu Li Li Hong Fan Liming Chen Jianqiong Zhang Zhongsheng Sun Xiao Han Zhenhua Dai Erguang Li M.Eric Gershwin Zhexiong Lian Xiong Ma Michael F.Seldin Weichang Chen Meilin Wang Xiangdong Liu Key Laboratory of Developmental Genes and Human Diseases School of Life Science and TechnologySoutheast UniversityNanjingJiangsu 210096China Institute of Translational Medicine Yangzhou University Medical CollegeYangzhouJiangsu 225009China Department of Radiology&Oncology The Second Affiliated Hospital of Soochow UniversitySuzhouJiangsu 215004China The First Affiliated Hospital of Soochow University SuzhouJiangsu 215006China Department of Bioinformatics School of Biomedical Engineering and InformaticsNanjing Medical UniversityNanjingJiangsu 210029China Author links open overlay panelYexi Huang Guangdong Provincial People's Hospital Guangdong Academy of Medical SciencesGuangzhouGuangdong 510080China Department of Gastroenterology and Hepatology Shanghai Institute of Digestive DiseasesShanghai Jiao Tong University School of Medicine Affiliated Renji HospitalShanghai 200001China Department of Laboratory Medicine The Fourth Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210031China Department of Gastrointestinal Endoscopy Eastern Hepatobiliary Surgery HospitalShanghai 201800China The Fifth People's Hospital Soochow UniversitySuzhouJiangsu 215007China Department of Gastroenterology Jiangsu University Affiliated Kunshan HospitalKunshanJiangsu 215300China Department of Gastroenterology Yixing People's HospitalYixinJiangsu 214200China Department of Gastroenterology Yancheng First People's HospitalYanchengJiangsu 224005China Department of Gastroenterology Taicang First People's HospitalSoochow UniversityTaicangJiangsu 215400China Department of Hepatology Jingjiang Second People's HospitalJingjiangJiangsu 214500China Department of Hepatology The Third People's Hospital of ChangzhouChangzhouJiangsu 213001China Department of Liver Diseases Shuguang HospitalShanghai University of Traditional Chinese MedicineShanghai 201203China Department of Laboratory Medicine Jiangyin People's HospitalSoutheast UniversityJiangyinJiangsu 214400China Department of Labora

Primary biliary cholangitis(PBC)is an autoimmune liver disease characterized by the destruction of intrahepatic small bile ducts and progressive cholestasis,eventually leading to liver cirrhosis and hepatic failure wi... 详细信息

关键词： cholangitis biliary

Label-Free Virtual HER2 Immunohistochemical Staining of Breast Tissue using Deep Learning

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biomedical {1. Frontiers 2022年第1期3卷 422-436页

作者： Bijie Bai Hongda Wang Yuzhu Li Kevin de Haan Francesco Colonnese Yujie Wan Jingyi Zuo Ngan B.Doan Xiaoran Zhang Yijie Zhang Jingxi Li Xilin Yang Wenjie Dong Morgan Angus Darrow Elham Kamangar Han Sung Lee Yair Rivenson Aydogan Ozcan Electrical and Computer engineering Department University of CaliforniaLos AngelesCA 90095USA Bioengineering Department University of CaliforniaLos Angeles 90095USA California NanoSystems Institute(CNSI) University of CaliforniaLos AngelesCAUSA Computer Science Department University of CaliforniaLos AngelesCAUSA Physics and Astronomy Department University of CaliforniaLos AngelesCA 90095USA Translational Pathology Core Laboratory University of CaliforniaLos AngelesCA 90095USA Statistics Department University of CaliforniaLos AngelesCA 90095USA Department of Pathology and Laboratory Medicine University of California at DavisSacramentoCA 95817USA Department of Surgery University of CaliforniaLos AngelesCA 90095USA

The immunohistochemical(IHC)staining of the human epidermal growth factor receptor 2(HER2)biomarker is widely practiced in breast tissue analysis,preclinical studies,and diagnostic decisions,guiding cancer treatment and investigation of ***2 staining demands laborious tissue treatment and chemical processing performed by a histotechnologist,which typically takes one day to prepare in a laboratory,increasing analysis time and associated ***,we describe a deep learning-based virtual HER2 IHC staining method using a conditional generative adversarial network that is trained to rapidly transform autofluorescence microscopic images of unlabeled/label-free breast tissue sections into bright-field equivalent microscopic images,matching the standard HER2 IHC staining that is chemically performed on the same tissue *** efficacy of this virtual HER2 staining framework was demonstrated by quantitative analysis,in which three board-certified breast pathologists blindly graded the HER2 scores of virtually stained and immunohistochemically stained HER2 whole slide images(WSIs)to reveal that the HER2 scores determined by inspecting virtual IHC images are as accurate as their immunohistochemically stained counterparts.A second quantitative blinded study performed by the same diagnosticians further revealed that the virtually stained HER2 images exhibit a comparable staining quality in the level of nuclear detail,membrane clearness,and absence of staining artifacts with respect to their immunohistochemically stained *** virtual HER2 staining framework bypasses the costly,laborious,and time-consuming IHC staining procedures in laboratory and can be extended to other types of biomarkers to accelerate the IHC tissue staining used in life sciences and biomedical workflow.

关键词： HER2 consuming Deep

枸杞总黄酮提取工艺优化及不同年份枸杞总黄酮含量测定

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西北民族大学学报（自然科学版） 2019年第3期40卷 70-75页

作者：刘元林龙鸣田晓静张福梅陈士恩马忠仁 NuruI Izza Nordin 西北民族大学生命科学与工程学院甘肃兰州730124 西北民族大学生物医学研究中心中国-马来西亚国家联合实验室甘肃兰州730030 Industrial Biotechnology Research Centre SIRIM Berhad1 Persiaran Dato' MenteriSection2 40700 Shah AlamSelangorMalaysia

为探究枸杞贮藏过程中总黄酮含量变化规律,以枸杞总黄酮提取率为指标,采用单因素实验研究浸提温度、乙醇体积分数、料液比和提取时间4因素对枸杞总黄酮提取率的影响,设计正交试验优化提取工艺,并在优化条件下研究不同年份枸杞总黄酮含... 详细信息

为探究枸杞贮藏过程中总黄酮含量变化规律,以枸杞总黄酮提取率为指标,采用单因素实验研究浸提温度、乙醇体积分数、料液比和提取时间4因素对枸杞总黄酮提取率的影响,设计正交试验优化提取工艺,并在优化条件下研究不同年份枸杞总黄酮含量及理化指标间的差异.结果发现,最佳提取工艺参数即浸提温度70℃、乙醇体积分数80%、料液比1.1.g/mL、提取时间2.5h、提取次数为2次.在此条件下,发现枸杞总黄酮含量随贮藏时间延长而呈现下降的变化趋势,这为枸杞总黄酮的开发利用和快速鉴别枸杞新陈提供理论依据.

关键词：枸杞黄酮工艺优化生产年份

Convalescent Plasma Antibody Levels and the Risk of Death from Covid-1.

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四川生理科学杂志 2021年第1期43卷 94-94页

作者： Michael J Joyner From the Departments of Anesthesiology and Perioperative Medicine (M.J.J. J.W.S. S.A.K. C.C.W. A.M.K. M.A.S. J.C.D.S. S.E.B. J.R.A.S. V.H. A.J.C. J.G.R. K.J.A. M.N.P.V. J.J.D. R.J.R.) Laboratory Medicine and Pathology (J.R.M. E.S.T. C.M.B. J.L.W. J.R.S.) and Cardiovascular Medicine (R.F.R. K.F.L. R.S.W.) the Human Research Protection Program (R.S.W.) and the Department of Internal Medicine Division of Pulmonary and Critical Care Medicine (P.R.B.) Mayo Clinic Rochester MN the Departments of Health Sciences Research (R.E.C. P.W.J. E.R.L. D.O.H.) and Cardiovascular Medicine (K.A.B. E.R.W. D.F.) Mayo Clinic Jacksonville FL the Department of Health Sciences Research (K.L.K. M.R.B.) and the Department of Internal Medicine Division of Infectious Diseases (J.E.B.) Mayo Clinic Phoenix AZ the Department of Anesthesiology Cooper Medical School of Rowan University Cooper University Health Care Camden NJ (N.H.) the center for Biologics Evaluation and Research Food and Drug Administration Silver Spring (N.C.V. P.M.) and the Department of Molecular Microbiology and Immunology Johns Hopkins Bloomberg School of Public Health Baltimore (A.C.) — both in Maryland and the Departments of Epidemiology and Biostatistics and of Pediatrics and Human Development College of Human Medicine Michigan State University East Lansing (N.S.P.).

Background:Convalescent plasma has been widely used to treat coronavirus disease 201.(Covid-1.)under the presumption that such plasma contains potentially therapeutic antibodies to severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)that can be passively transferred to the plasma *** convalescent plasma with high antibody levels rather than low antibody levels is associated with a lower risk of death is ***:In a retrospective study based on a *** registry,we determined the anti-SARS-CoV-2 IgG antibody levels in convalescent plasma used to treat hospitalized adults with Covid-1..

关键词： Plasma transferred retrospective