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生物钟蛋白Bmal1对实验性牙周炎相关肾损伤的影响

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华西口腔医学杂志 2024年第2期42卷 163-171页

作者：马浩楠李琼商雅琦辛禧瑞刘歆婵武洲于维先吉林大学口腔医院牙周病科长春130021 吉林大学口腔医院种植科长春130021 日本九州大学齿学研究院日本福冈812-8582 吉林大学口腔医院老年口腔科长春130021 吉林省口腔生物医学国际联合研究中心长春130021

目的通过建立大鼠牙周炎模型,探讨生物钟蛋白Bmal1对慢性牙周炎相关肾损伤的影响。方法将12只雄性Wistar大鼠随机分为对照组和牙周炎组,每组6只。采用正畸结扎丝对牙周炎组大鼠双侧上颌第一磨牙进行结扎处理,对照组大鼠不进行任何干预... 详细信息

目的通过建立大鼠牙周炎模型,探讨生物钟蛋白Bmal1对慢性牙周炎相关肾损伤的影响。方法将12只雄性Wistar大鼠随机分为对照组和牙周炎组,每组6只。采用正畸结扎丝对牙周炎组大鼠双侧上颌第一磨牙进行结扎处理,对照组大鼠不进行任何干预措施。8周后,检测2组大鼠的牙周临床指标,包括牙周探诊深度、牙龈出血指数和牙齿松动度。采用Micro-CT对大鼠上颌骨进行扫描和三维图像重建,评估牙槽骨吸收情况。采用苏木精-伊红(HE)和过碘酸雪夫(PAS)染色对牙周组织和肾组织进行病理学观察。采用生化试剂盒检测肾脏功能指标肌酐、白蛋白、血尿素氮的水平,以及氧化应激指标超氧化物歧化酶、谷胱甘肽和丙二醛的水平。MitoSOX red染色检测肾组织内活性氧(ROS)含量。实时荧光定量聚合酶链反应(RT-qPCR)和免疫组织化学染色检测大鼠肾组织中Bmal1、核因子-E2相关因子2(Nrf2)和血红素加氧酶1(HO-1)的基因及蛋白表达水平。结果与对照组相比,牙周组织Micro-CT及HE染色结果显示,牙周炎组上颌第一磨牙区出现明显的骨吸收和附着丧失;肾组织HE及PAS染色结果表明,牙周炎组大鼠肾组织有显著的组织病理损伤;肾功能和氧化应激指标结果显示,牙周炎组的氧化应激水平出现异常而肾功能指标无明显异常;MitoSOX red结果显示,牙周炎组肾组织内ROS含量升高;RT-qPCR和免疫组织化学结果显示,牙周炎组肾组织内Bmal1、Nrf2和HO-1表达水平降低。结论生物钟蛋白Bmal1在牙周炎大鼠肾脏的氧化损伤过程中发挥重要作用。

关键词：牙周炎肾损伤昼夜节律氧化应激生物钟蛋白Bmal1

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线粒体解偶联蛋白2在大鼠实验性牙周炎相关肾损伤中的作用研究

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华西口腔医学杂志 2024年第4期42卷 502-511页

作者：李琼马浩楠商雅琦辛禧瑞刘歆婵武洲于维先吉林大学口腔医院牙周病科长春130021 吉林大学口腔医院种植科长春130021 日本九州大学齿学研究院日本福冈812-8582 吉林大学口腔医院老年口腔科长春130021 吉林省牙发育及颌骨重塑与再生重点实验室长春130021

目的探索线粒体解偶联蛋白2(UCP2)在结扎诱导的实验性牙周炎相关肾损伤中的变化,研究UCP2对牙周炎诱导肾损伤的影响。方法选取12只Wistar雄性大鼠随机分为2组:对照组和牙周炎组。使用0.2 mm正畸结扎丝结扎在大鼠上颌第一磨牙的牙颈部,... 详细信息

目的探索线粒体解偶联蛋白2(UCP2)在结扎诱导的实验性牙周炎相关肾损伤中的变化,研究UCP2对牙周炎诱导肾损伤的影响。方法选取12只Wistar雄性大鼠随机分为2组:对照组和牙周炎组。使用0.2 mm正畸结扎丝结扎在大鼠上颌第一磨牙的牙颈部,建立牙周炎模型。8周后观察2组大鼠口内情况,检测牙周临床指标牙龈出血指数(BI)、牙周探诊深度(PD)、牙齿松动度(TM)。收集大鼠上颌骨并扫描Micro CT,观察牙槽骨吸收情况,记录组织矿物质密度(TMD)、骨矿物质密度(BMD)、骨体积分数(BV/TV)、骨小梁厚度(***)、骨小梁骨分离度(***)等参数,测量上颌第一磨牙釉牙骨质界到牙槽嵴顶的距离(CEJ-ABC)。冻存大鼠肾组织以检测氧化应激指标丙二醛(MDA)、谷胱甘肽(GSH)、超氧化物歧化酶(SOD),并进行实时定量聚合酶链反应(qRT-PCR)观察UCP2、核因子红系2相关因子2(Nrf2)抗体、过氧化物酶体增殖物激活受体γ辅激活因子-1α(PGC-1α)基因表达情况。使用大鼠牙龈组织进行免疫组织化学染色,观察牙龈组织UCP2表达情况。使用大鼠固定的肾组织进行苏木精-伊红(HE)、过碘酸雪夫(PAS)、MitoSOX Red、JC-1、免疫组织化学染色,观察大鼠肾组织病理学表现、活性氧(ROS)水平、线粒体膜电位水平及UCP2、Nrf2、PGC-1α蛋白表达情况。收集大鼠血清检测肾脏功能指标血尿素氮(BUN)、肌酐(Cre)、白蛋白(Alb)的表达。结果相较于对照组,牙周炎组大鼠第一磨牙周围的牙龈组织发红肿胀,质地松软,临床检查探诊出血且PD增加,牙齿松动,牙槽骨吸收,TMD、BMD、BV/TV、***指数降低,***指数及CEJ-ABC增加,牙龈UCP2蛋白表达升高。相较于对照组,牙周炎组大鼠肾组织内MDA、ROS水平上升,MMP、GSH和SOD水平下降,UCP2基因及蛋白表达升高,Nrf2、PGC-1α基因及蛋白表达下降。肾脏功能指标BUN、Cre、Alb两组差异无统计学意义。结论UCP2可通过氧化应激在牙周炎诱导的肾损伤中发挥作用。

关键词：牙周炎肾损伤线粒体解偶联蛋白2

三维DDA方法的验证及其在隧道围岩垮塌控制中的应用

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东南大学学报（自然科学版） 2024年第3期54卷 531-538页

作者：李利平范宏运刘洪亮周申高靖遥张延欢山东大学齐鲁交通学院济南250001 九州大学土木与结构工程系日本福冈812-0044

为准确模拟隧道围岩垮塌灾害演化过程与锚杆加固处置,通过嵌入锚杆支护算法实现了三维非连续变形分析方法(DDA)中的锚杆支护模拟.首先,介绍了三维DDA方法模拟块体运动的基本原理,提出了三维DDA方法中锚杆支护算法;其次,针对隧道围岩垮... 详细信息

为准确模拟隧道围岩垮塌灾害演化过程与锚杆加固处置,通过嵌入锚杆支护算法实现了三维非连续变形分析方法(DDA)中的锚杆支护模拟.首先,介绍了三维DDA方法模拟块体运动的基本原理,提出了三维DDA方法中锚杆支护算法;其次,针对隧道围岩垮塌灾害中的不同运动模式,采用三维DDA方法模拟了块体滑移、转动以及碰撞过程,验证了三维DDA方法模拟块体运动的准确性;最后,采用三维DDA方法模拟了工程尺度的节理岩体隧道围岩垮塌过程.结果表明:无支护条件下隧道洞周块体垮塌高度为3.9 m,洞周块体位移呈现出明显的各向异性特征;有支护条件下隧道洞周块体保持稳定状态,隧道左拱肩部位锚杆轴力较大,实际施工过程中可增加该区域锚杆长度.

关键词：三维非连续变形分析方法(DDA) 围岩垮塌稳定性分析锚杆支护

Myocardial metastasis from ZEB1-and TWIST-positive spindle cell carcinoma of the esophagus:A case report

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World Journal of Gastroenterology 2024年第11期30卷 1636-1643页

作者： Yoshihiro Shibata Hirofumi Ohmura Kazuki Komatsu Kosuke Sagara Atsuji Matsuyama Ryuji Nakano Eishi Baba Department of Medical Oncology Fukuoka Wajiro HospitalFukuoka 811-0213Japan Department of Oncology and Social Medicine Kyushu University Graduate School of Medical SciencesFukuoka 812-8582Japan Department of Pathology and Oncology University of Occupational and Environmental HealthKitakyushu 807-8555Japan Department of Diagnostic Pathology Fukuoka Wajiro HospitalFukuoka 811-0213Japan

BACKGROUND Metastatic cardiac tumors are known to occur more frequently than primary cardiac tumors,however,they often remain asymptomatic and are commonly dis-covered on *** tumors with a relatively high frequency of cardiac metastasis include mesothelioma,melanoma,lung cancer,and breast cancer,whereas reports of esophageal cancer with cardiac metastasis are *** SUMMARY The case of a 60-year-old man who complained of dysphagia is *** gastrointestinal endoscopy showed a submucosal tumor-like elevated lesion in the esophagus causing ***-enhanced computed tomography showed left atrial compression due to the esophageal tumor,multiple liver and lung metastases,and a left pleural *** examination of a biopsy speci-men from the esophageal tumor showed spindle-shaped cells,raising suspicion of esophageal *** disease progressed rapidly,and systemic chemotherapy was deemed necessary,however,due to his poor general condition,adminis-tration of cytotoxic agents was considered *** his high Combined Positive Score,nivolumab was administered,however,the patient soon died from the *** autopsy confirmed spindle cell carcinoma(SCC)of the esophagus and cardiac metastasis with similar histological *** stem cell markers,ZEB1 and TWIST,were positive in both the primary tumor and the cardiac *** To the best of our knowledge,there have been no prior reports of cardiac metastasis of esophageal *** case highlights our experience with a patient with esophageal SCC who progressed rapidly and died from the disease,with the autopsy examination showing cardiac metastasis.

关键词： Spindle cell carcinoma Esophagus Myocardial metastasis Epithelial-mesenchymal transition Case report

Two-Stream Approximation to the Radiative Transfer Equation:A New Improvement and Comparative Accuracy with Existing Methods

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Advances in Atmospheric Sciences 2024年第2期41卷 278-292页

作者： F.Momo TEMGOUA L.Akana NGUIMDO DNJOMO Environmental Energy Technologies Laboratory(EETL) Faculty of SciencesUniversity of YaoundéPO Box 812 YaoundéCameroon Department of Electrical and Electronic Engineering Faculty of Engineering and TechnologyUniversity of BueaPO Box 63 BueaCameroon

Mathematical modeling of the interaction between solar radiation and the Earth's atmosphere is formalized by the radiative transfer equation(RTE), whose resolution calls for two-stream approximations among other methods. This paper proposes a new two-stream approximation of the RTE with the development of the phase function and the intensity into a third-order series of Legendre polynomials. This new approach, which adds one more term in the expression of the intensity and the phase function, allows in the conditions of a plane parallel atmosphere a new mathematical formulation of γparameters. It is then compared to the Eddington, Hemispheric Constant, Quadrature, Combined Delta Function and Modified Eddington, and second-order approximation methods with reference to the Discrete Ordinate(Disort) method(δ –128 streams), considered as the most precise. This work also determines the conversion function of the proposed New Method using the fundamental definition of two-stream approximation(F-TSA) developed in a previous work. Notably,New Method has generally better precision compared to the second-order approximation and Hemispheric Constant methods. Compared to the Quadrature and Eddington methods, New Method shows very good precision for wide domains of the zenith angle μ 0, but tends to deviate from the Disort method with the zenith angle, especially for high values of optical thickness. In spite of this divergence in reflectance for high values of optical thickness, very strong correlation with the Disort method(R ≈ 1) was obtained for most cases of optical thickness in this study. An analysis of the Legendre polynomial series for simple functions shows that the high precision is due to the fact that the approximated functions ameliorate the accuracy when the order of approximation increases, although it has been proven that there is a limit order depending on the function from which the precision is lost. This observation indicates that increasing the order of app

关键词： Radiative Transfer Equation two-stream method Legendre polynomial optical thickness moments of specific intensity conversion function transmittance reflectance

Effects of pore interconnectivity on bone regeneration in carbonate apatite blocks

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Regenerative Biomaterials 2022年第1期9卷 30-40页

作者： Maab Elsheikh Ryo Kishida Koichiro Hayashi Akira Tsuchiya Masaya Shimabukuro and Kunio Ishikawa Department of Biomaterials Faculty of Dental ScienceKyushu University3-1-1 Maidashi Higashi-kuFukuoka 812-8582Japan

Porous architecture in bone substitutes,notably the interconnectivity of pores,is a critical factor for bone ***,controlling the pore interconnectivity while maintaining the microarchitecture has not yet been achieved using conventional methods,such as ***,we fabricated a porous block using the crystal growth of calcium sulfate dihydrate,and controlled the pore interconnectivity by limiting the region of crystal *** calcium sulfate dihydrate blocks were transformed to bone apatite,carbonate apatite(CO_(3)Ap)through dissolution–precipitation ***,CO_(3)Ap blocks with 15%and 30%interconnected pore volumes were obtained while maintaining the microarchitecture:they were designated as CO_(3)Ap-15 and CO_(3)Ap-30,*** 4 weeks after implantation in a rabbit femur defect,new bone formed throughout CO_(3)Ap-30,whereas little bone was formed in the center region of CO_(3)*** 12 weeks after implantation,a large portion of CO_(3)Ap-30 was replaced with new bone and the boundary with the host bone became *** contrast,CO_(3)Ap-15 remained in the defect and the boundary with the host bone was still ***,the interconnected pores promote bone ingrowth,followed by replacement of the material with new *** findings provide a useful guide for designing bone substitutes for rapid bone regeneration.

关键词： bone substitutes carbonate apatite pore interconnectivity bone regeneration

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如果当时没犹豫就好了

作文新天地 2024年第11期 8-8页

作者：杨佳婧浙江省金华市磐安县新城中学812班

“如果当时没犹豫就好了。”你躺在床上，不断回想着。你仍记得，犹豫是否扶起摔倒的老奶奶，你看她独自爬起，默默理好散落的东西。你仍记得，犹豫是否为遭受校园暴力的同学发声，你看她从活泼变得沉默。你仍记得，犹豫是否做志愿者，...

“如果当时没犹豫就好了。”你躺在床上，不断回想着。你仍记得，犹豫是否扶起摔倒的老奶奶，你看她独自爬起，默默理好散落的东西。你仍记得，犹豫是否为遭受校园暴力的同学发声，你看她从活泼变得沉默。你仍记得，犹豫是否做志愿者，你看朋友圈的照片里洋溢着笑容的全是别人。

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小鼠胎肝激酶-1阳性细胞向胆管细胞和肝细胞分化

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中华肝脏病杂志 2010年第7期18卷 544-545页

作者：金成豪米满吉和居石克夫时军江西省人民医院血液内科南昌330006 Division of Pathophysiological and ExperimentaJ Pathology Department of Pathology Graduate School of Medical Sciences Kyushu University Fukuoka 812 8582 JapanDepartmentofPathologyGraduateSchoolofMedicalSciencesKyushuUniversityFukuoka812-8582Japan

干细胞对再生医学和人工器官的发展具有巨大潜在应用价值.肝脏可能是再生医学的一个很有吸引力的目标,理由是：（1）肝脏是一种多种遗传性和获得性代谢性疾病的易损器官;（2）增殖肝可接受来自同基因供者的肝细胞.

关键词：干细胞小鼠胎肝激酶-1

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日本人多发性硬化的流行病学和免疫学特征

中华神经科杂志 2009年第6期42卷 422-424页

作者：吉良潤一王朝东(译) 亍812-8582 福岡市東区馬出3-1-1九州大学大学院医学研究院神经内科江西省人民医院神经内科南昌330006

多发性硬化（MS）是一种机制未明的中枢神经系统自身免疫性脱髓鞘性疾病，欧美人群多见，亚洲一直被认为是MS的低发区，但随着居住环境、生活习惯等的变化，以及诊断手段的提高，越来越多的亚洲病例被报道。近年来，国际上有关MS的发病... 详细信息

多发性硬化（MS）是一种机制未明的中枢神经系统自身免疫性脱髓鞘性疾病，欧美人群多见，亚洲一直被认为是MS的低发区，但随着居住环境、生活习惯等的变化，以及诊断手段的提高，越来越多的亚洲病例被报道。近年来，国际上有关MS的发病机制和治疗研究取得了不少新的进展，呈现出一些新的趋势。我们及日本其他研究小组对本国MS的流行病学、临床特征、免疫遗传学基础和免疫学机制等进行了广泛研究，得到了一些新的结果。现就有关流行病学和免疫学特征的部分研究结果汇报如下。

关键词：免疫学特征多发性硬化流行病学日本人脱髓鞘性疾病中枢神经系统自身免疫性免疫学机制