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Resistance index and browning mechanism of apple peel under high temperature stress

Horticultural Plant Journal 2024年第2期10卷 305-317页

作者： Hui Wang Shuhui Zhang Zidun Wang Dongmei Li Leiyu Yan Yifeng Feng Xiaojie Liu Rongxin Chen Wenmin Fan Lulong Sun Zhengyang Zhao college of Horticulture Northwest A&F UniversityYanglingShaanxi 712100China college of Horticultural Science and engineering Shandong Agricultural University State Key Laboratory of Crop BiologyTai'anShandong 271018China

Apples are one of the most important economic crops *** of global warming and an aggravation of environmental,abnormally high temperatures occur frequently in fruit-growing season and seriously affect normal fruit growth and reduce fruit quality and *** took five-year-old Ruixue’(Qinfu 1×Pink Lady;CNA20151469.1) fruits as test materials,and the ambient temperature during fruit development was *** results showed that during the fruit-growing season,especially during the rapid growth stage (July to August),the maximum daily temperature exceeded 30℃ and lasted for more than 40 *** determine the effects of high temperature stress on the apple fruit resistance,we treated expanding,veraison,and maturity-period fruits at different *** was found that the fruits of the expanding period showed strong resistance to high temperature stress,whereas during veraison and maturity,fruit resistance to high temperature stress decreased,and the fruit peel browning phenotype ***,the content of malonaldehyde (MDA),hydrogen peroxide (H_(2)O_(2)),and superoxide anion (O._(2)^(-)) in the peel gradually increased with increasing *** content of total phenols,flavanol,and flavonoids in the peel decreased substantially at 45℃.Moreover,it was found that polyphenol oxidase gene (MdPPO1) was most sensitive to high temperature stress in ***,transient and stable MdPPO1 overexpression significantly promoted peel *** transgenic materials were more sensitive to high temperatures,and browning was more severe compared to non-genetically modified organism (WT).Stable MdPPO1 knockout calli obtained via clustered regularly interspersed short palindromic repeats (CRISPR/Cas9) gene knockout technology reduced the browning phenotype,and the resultant fruits were not sensitive to the effects of high temperature ***,MdPPO1 expression may be a key factor of high temperature-related changes observed in the browni

关键词： Malus domestica Borkh High temperature stress Peel Browning MdPPO1

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One-Pot Stereoselective Synthesis of Different Fused Multicyclic Iminosugars Based on the Iminium-lon Intermediate

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Chinese Journal of Chemistry 2024年第2期42卷 142-150页

作者： Song Xie Jilai Wu Likai Zhou Chao Wei Xiaoliu Li Hua Chen Key Laboratoryof Chemical biology of Hebei Province College of Chemistry and Material ScienceHebei UniversityBaodingHebei 071002China college of Chemistry and Chemical engineering Xingtai UniversityXingtaiHebei 054001China

Different novel fused multicyclic iminosugars were synthesized from D-ribose tosylate,aniline and vinyl ethyl ether by one-pot three-component stereoselective[4+2]reaction at different *** iminium-ion is the key intermediate for the *** a result,several complex fused iminosugars 3a were obtained by aza-Diels-Alder mechanism at 60℃,while a series of aza-C-glycosides 5a were prepared by Mannich reaction at room temperature accompanied by another tetrahydroquinoline-fused iminosugars 4a(tricyclic derivatives)through aza-Diels-Alder *** strategy will help to construct structurally diverse and bioactive iminosugar analogues.

关键词： Fused multicyclic iminosugar Aza-Diels-Alder cycloaddition Mannich reaction Tetrahydroquinoline Multicomponent reactions Enantioselectivity Cyclization

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Oxygen heterocyclic compounds(OHCs) in lemon oils are important markers in targeted HPLC analyses for authentication

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food Science and Human Wellness 2024年

作者： Siyu Liu Shiming Li Chi-Tang Ho Department of food Science， Rutgers University New Brunswick college of biology and Agricultural Resources， Huanggang Normal University

Lemon oils are broadly used as flavoring agents in beverages， foods， cosmetics and pharmaceuticals， yet the adulteration of natural， particularly cold pressed lemon oils is very common in the industry due to its unmet demand and high cost. Nowadays， most quality control (QC) analysis of lemon oils is conducted by gas chromatography (GC) analysis， which is far from a reliable method. Oxygen heterocyclic compounds (OHCs) in non-volatile fraction are gaining increasing attention in authentication process because of the nearly finger-printing profiles of OHCs in cold pressed citrus essential oils. Our goal in this study was to identify OHCs using high performance liquid chromatography (HPLC) in lemon oils， establish OHC profiles， perform stepwise logistic regression analysis (SLRA) and build effective predicting model and further determine adulterated lemon oils by referencing the OHC profiles and established models. After HPLC analyses， profiling and SLRA modeling of 154 OHCs samples of industrial lemon oils， we found that the combination of isopimpinellin and total OHC concentration are essential and robust predictors to differentiate authentic samples from adulterated lemon oils with a success rate of 98% from the 5-fold cross validation. This study provided a reliable and efficient method in determining the authenticity of lemon oils.

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Unraveling the trans-epithelial absorption of novel dark blue pigment from Vaccinium bracteatum Thunb. leaves on the Caco-2 intestinal cell

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food Science and Human Wellness 2024年

作者： Mingcong Fan Weijia Lian Wenjun Yao Tingting Li Jiajia Zhao Qiang Li Yanming Guan Yan Li Haifeng Qian Zhiming Rao Li Wang School of food Science and Technology， State Key Laboratory of food Science and Technology， National engineering Research Center for Functional food， Jiangnan University college of Cooking Science and Technology， Jiangsu college of Tourism School of Biotechnology， Jiangnan University Department of food Science and engineering， college of Light Industry and food engineering， Nanjing Forestry University China National Research Institute of food and Fermentation Industries Co.Ltd China National Institute of Standardization

The dark blue pigment (DBP) is a health ingredient from Vaccinium bracteatum Thunb. leaves used as a functional food supplement. However， the details of transepithelial absorption on the intestinal epithelial cells are barely understood. This study aimed to clarify the absorption properties of DBP in the Caco-2 cell monolayer model and evaluate the effect on the endo-metabolism and barrier function of Caco-2 cells. The results showed that the DBP did not show the dose-dependent toxic effect to Caco-2 cells between 0.25 and 1.5 mg/mL， which did not cause disorder in the normal cell metabolism and absorption activity. The Caco-2 cells monolayer model could absorb DBP by passive and active transport， and the absorptive pattern was dose-dependent when the concentration was more than 0.25 mg/mL. During DBP absorption， an increase in mRNA and protein expressions of glucose transporters demonstrated that the glucose transporters were the potential transporter of DBP. But the glucose transport amounts were significantly lowered after 30 min of DBP treatment， indicating that DBP owned the inhibitory effect on glucose transportation. Furthermore， DBP also owned protective effects on the barrier function of intestinal epithelial cells.

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Weizmannia coagulans spores alleviates DSS-induced ulcerative colitis model by modulation of gut flora， metabolites and suppressing TLR4/MyD88/NF-κB pathway

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food Science and Human Wellness 2024年

作者： Ying Wu Pingping Tian Junfei Li Han Gao Shanshan Tie Shumiao Zhao Huayi Suo Shaobin Gu college of food and Bioengineering， Henan University of Science and Technology National Demonstration Center for Experimental food Processing and Safety Education Henan engineering Research Center of food Microbiology college of food Science， Southwest University college of Life Science and Technology， Huazhong Agricultural University

Ulcerative colitis (UC) is a chronic intestinal inflammatory disease characterized by a complex pathogenesis. Weizmannia coagulans has emerged as a potential probiotic for treating intestinal disorders. This study aimed to assess the therapeutic impact of Weizmannia coagulans BC99 on mice with DSS-induced UC and to elucidate its underlying mechanism of action. Our findings revealed that BC99 administration ameliorated symptoms associated with DSS-induced UC mice， as evidenced by reduced disease activity indexes， reversal of weight loss， and normalization of colon length. Furthermore， BC99 treatment also protected the integrity of the intestinal barrier through maintaining the antioxidant activity and the expression of tight junction proteins (ZO-1 and occluding)， and regulating the inflammatory cytokines in DSS-induced UC mice. Additionally， BC99 supplementation enhanced the production of short-chain fatty acids (SCFAs) through the proliferation of SCFA-producing bacteria， including Bidobacterium， Blautia and Faecallbaculum. Notably， the NF-κB signaling pathway was found to be closely related to BC99 treatment in DSS-induced UC mice. The positive protein expression the mRNA expression of TLR4， MyD88 and p65 in colon tissue were all detected in BC99-treated groups， which indicating that BC99 could alleviate UC symptoms by inhibiting TLR4/MyD88/NF-κB signaling pathway. Metabolomics further confirms the previous results. Collectively， these findings provide basic support for the Weizmannia coagulans as a functional food additive or a promising therapeutic agent for the effective management of UC.

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Modeling method for SSC prediction in pomelo using Vis-NIRS with wavelength selection and latent variable updating

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International Journal of Agricultural and Biological engineering 2024年第1期17卷 251-260页

作者： Hao Tian Shuai Wang Huirong Xu Yibin Ying college of Biosystems engineering and food Science Zhejiang UniversityHangzhou 310058China Key Laboratory of On-Site Processing Equipment for Agricultural Products Ministry of Agriculture and Rural AffairsHangzhou 310058China college of food Science Shihezi UniversityShihezi 832003XinjiangChina

The aim of this study was in-line,rapid,and non-destructive detection for soluble solid content(SSC)in pomelos using visible and near-infrared spectroscopy(Vis-NIRS).However,the large size and thick rind of pomelo affect the stability of spectral acquisition and the biological variabilities affect the robustness of *** these issues,in this study,an efficient prototype in-line detection system in transmittance mode was designed and evaluated in comparison with an off-line detection *** from the years 2019 and 2020 were used for modeling and the external validation data were obtained by the inline detection system in *** wavelength selection methods of changeable size moving window(CSMW),random frog(RF),and competitive adaptive reweighted sampling(CARS)were used to improve the prediction accuracy of partial least squares regression(PLSR)*** best performance of internal prediction was obtained by CARS-PLSR and the determination coefficient of prediction(),root mean square error of prediction(RMSEP),and residual predictive deviation(RPD)were 0.958,0.204%,and 4.821,***,all models obtained large prediction biases in external *** latent variable updating(LVU)method was proposed to update models and improve the performance in external *** samples from the external validation set were randomly selected to update the *** with the recalibration method,LVU could effectively modify the original models which matched the SSC range of the external validation *** CSMW-PLSR models were more robust in external *** off-line model with LVU performed best with a root mean square error of validation(RMSEV)of 0.599%and the in-line model with recalibration obtained RMSEV of 0.864%.These results demonstrated the application potential of the transmittance Vis-NIRS for in-line rapid prediction of SSC in pomelos and the modeling and updating methods could be applied to samples with biological variabi

关键词： Vis-NIRS in-line detection external validation wavelength selection model updating pomelo SSC

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Multifunctional carbon materials from rugose rose for energy storage and water purification

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Frontiers of Chemical Science and engineering 2024年第9期18卷 1-13页

作者： Peng-Hui Li Hui Zhou Wen-Juan Wu Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources Nanjing Forestry UniversityNanjing 210037China college of Light Industry and food engineering Nanjing Forestry UniversityNanjing 210037China college of Chemical engineering East China University of Science and TechnologyShanghai 200237China State Key Laboratory of Chemical engineering Shanghai 200237China

With the advancement of social process,the resource problem is becoming more prominent,biomass materials come into being,and it is becoming more and more important to explore and prepare efficient and multifunctional biomass materials to alleviate the problems of energy storage and water *** this paper,nitrogen-doped hierarchical porous carbon materials(NRRC)were produced by one-step carbonization of withered rose as raw material and melamine as nitrogen source with KOH-activated *** resulting nitrogen-doped porous carbon material had the most abundant pores and the best microspherical graded pore structure,with a specific surface area of up to 1393 m2·g^(-1),a pore volume of 0.68 cm3·g^(-1),and a nitrogen-doped content of 5.52%.Electrochemical tests showed that the maximum specific capacitance of NRRC in the three-electrode system was 346.4 F·g^(-1)(0.5 A·g^(-1)),which was combined with favorable capacitance retention performance and cycling *** NRRC//NRRC symmetric supercapacitors were further assembled,and the maximum energy density of a single device was 23.88 Wh·kg^(-1),which still maintains excellent capacitance retention and cyclic charging/discharging *** example,the capacitance retention rate was always close to 96.27%with almost negligible capacitance loss after 10000 consecutive charge/discharge cycles(current density:10 A·g^(-1)).Regardless of the three-electrode or two-electrode system,the super capacitive performance of NRRC porous carbon materials was comparable to the electrochemical performance of many reported biomass porous carbon materials,which showed better energy storage advantages and practical application *** addition,NRRC porous carbon materials had excellent water purification *** dye adsorption test confirmed that NRRC had a high adsorption capacity(491.47 mg·g^(-1))for methylene *** undoubtedly also showed a potential and promising avenue for high value-added utilization

关键词： rose carbon adsorption electrode material electrochemical performance

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Understanding the mechanism underlying the anti-inflammatory effect of dietary flavonoids on IBD: a focus on gut microbiota and its metabolites involved in SCFA， bile acid， tryptophan and lipopolysaccharides

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food Science and Human Wellness 2024年

作者： Huihui Xiong Hui Chen Lingli Chen Kehui Ouyang Suyun Lin Wenjun Wang college of food Science and engineering， Jiangxi Agricultural University college of Animal Science and Technology， Jiangxi Agricultural University

Inflammatory bowel disease (IBD) is a chronic gastrointestinal disease with a high incidence. Treatment for IBD includes medications and diet， and common anti-inflammatory medications have limitations like drug resistance and serious adverse effects. Accumulating evidence has demonstrated that dietary flavonoids exhibit an alleviative effect on IBD by influencing gut microbiota. The microbiota-derived metabolites also regulate IBD and maintain intestinal homeostasis. In this review， we investigate the therapeutic effect of gut microbiota and metabolites on IBD by intestinal immune and intestinal barrier function. We demonstrate the underlying mechanism of dietary flavonoids as an anti-inflammatory molecule alleviating IBD by regulating gut microbiota， short chain fatty acid (SCFA)， bile acid (BA)， tryptophan (Trp) metabolism and lipopolysaccharides (LPS)-toll-like receptor 4 (TLR4) signaling pathway. Based on structural differences of flavonoids， we summarize the recent research progress on the role of different dietary flavonoids in alleviating IBD by gut microbiota and metabolites in animal and clinical trials. This review indicates that dietary flavonoids targeting gut microbiota and metabolites provide a promising strategy for the treatment of inflammation and novel insights into the management of IBD.

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Efficient and Fast X-Ray Luminescence in Organic Phosphors Through High-Level Triplet-Singlet Reverse Intersystem Crossing

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CCS Chemistry 2024年第2期6卷 334-341页

作者： Yang Zhang Minghong Chen Xiaoze Wang Miao Lin Hongyu Wang Weihong Li Fuhai Chen Qing Liao Hongming Chen Qiushui Chen Meijin Lin Huanghao Yang Key Laboratory of Molecule Synthesis and Function Discovery College of ChemistryFuzhou UniversityFuzhouFujian 350116 New Cornerstone Science Laboratory MOE Key Laboratory for Analytical Science of Food Safety and BiologyCollege of ChemistryFuzhou UniversityFuzhouFujian 350108 college of Materials Science and engineering Fuzhou UniversityFuzhouFujian 350116

Organic scintillators that efficiently generate bright triplet excitons are of critical importance for highperformance X-ray-excited luminescence in radiation ***,the nature of triplet-singlet spinforbidden transitions in these materials often result in long-lived phosphorescence,which is undesirable for ultrafast X-ray detection and *** we demonstrate that the effect of hybridized local and charge-transfer(HLCT)excited states enables organic scintillators to exhibit highly efficient and fast radioluminescence(RL)in response to X-ray *** experimental and theoretical investigation shows that the oxidized 1,8-naphthalimide-phenothiazine dyad(OMNI-PTZ 2)with HLCT-excited states has an enhanced overlap integral of the highest occupied molecular orbital(HOMO)and lowest unoccupied molecular orbital(LUMO)on MNIπ-orbitals,and moderate donor–acceptor electron *** a result,the RL of these crystals exhibits a 61-fold increase and its monoexponential decay lifetime is three orders of magnitude faster compared to its corresponding thermally activated delayed fluorescence(TADF)molecule MNI-PTZ *** further demonstrate the practical utility of the OMNI-PTZ 2(G)in high-performance X-ray detection and imaging,achieving an X-ray dose sensitivity of 97 nGy s−1 and an exceptional spatial resolution of 20 lp/*** study provides a promising molecular design principle for utilizing triplet excitons to develop high-efficiency and fast X-ray scintillators for the development of next-generation flexible and stretchable X-ray imaging detectors.

关键词： organic scintillators X-ray imaging hybridized local and charge transfer excited state triplet-singlet reverse intersystem crossing high exciton utilization

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Effect of length-width ratio of rounded rectangle aquaculture tank in dual-diagonal-inlet layout on hydrodynamics

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Journal of Oceanology and Limnology 2024年第5期42卷 1695-1709页

作者： Meng LI Xiaozhong REN Shupeng DU Wei SUN Chenxu ZHAO Hangfei LIU Xianying SHI Key Laboratory of Environment Controlled Aquaculture(Dalian Ocean University) Ministry of EducationDalian 116023China college of Biosystems engineering and food Science(BEFS) Zhejiang UniversityHangzhou 310058China college of Ocean and Civil engineering Dalian Ocean UniversityDalian 116023China

To adapt to the change of aquaculture workshop site,optimize the shape of aquaculture tanks and improve the utilization rate of breeding space,it is necessary to determine the appropriate length width ratio parameters of aquaculture *** this paper,computational fluid dynamics(CFD)technology is adopted to study the flow field performance of aquaculture tanks with different L/B ratios(L:the length;B:the width,of aquaculture tank)and different jet direction conditions(lengthways jet and widthways jet).A three-dimensional numerical calculation model of turbulence in rounded rectangle aquaculture tanks in dual-diagonal-inlet layout was *** directions are arranged lengthways and widthways,and the water flow velocity,resistance coefficient change,vorticity,*** analyzed under two working *** show that the flow field performance in aquaculture tank decreases with the increase of the L/B *** flow field performed well when L/B was 1.0-1.3,sharply dropped at 1.4-1.6,and poor at *** results provided a theoretical basis for the design and optimization in flow field performance of the industrialized circulating aquaculture tanks.

关键词： aquaculture tank length-width ratio computational fluid dynamic hydrodynamic

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