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American Journal of Plant Sciences 2018年第11期9卷 2205-2216页

作者： Haiko Enok Sawazaki Valdir Atsushi Yuki Marlon Ricardo Alvez Ortiz Walter Hissao Banja Julio Massaharu Marubayashi Karoline S. Fonseca Geovanni A. Luchini Renan G. Perroni Wallison M. O. Andrade Instituto Agronô mico-APTA Campinas Brazil Hortec Sementes Ltda Bragan&ccedil a Paulista Brazil CNPq/PIBITI Scholarship Training Campinas Brazil

Screening for the source of virus resistance in horticultural plants or specific characterization as hybridization, through symptoms, requires time and depends on the weather and knowledge of plant characteristics. So, it is important to develop specific gene markers to allow rapid diagnosis by PCR. Markers were developed based on sequences homology comparison of susceptible and resistant plants provided by HORTEC SEEDS in tomato for Tomato yellow leaf curl virus (TYLCV) by the resistance gene Ty-1, in zucchini for zucchini yellow mosaic virus (ZYMV) and Papaya ringspot virus estirpe watermelon (PRSV-W), and in lettuce for Lettuce mosaic virus (LMV). Fragments of 249 bp were amplified only by resistant plants to TYLCV as the hybrids 2648 and Aguamiel, and not for varieties as Santa Cruz or Carina. It were observed for ZYMV the amplification of 791 bp by the resistant hybrid Px7051 and not for the susceptible cultivar La Belle;for PRSV-W using the same zucchini plants the amplification of 650 bp for susceptible and 750 bp for resistant;for LMV the 421 bp amplification only for the resistant cultivar Brasil 303 and not for susceptible Babá de Verão. Finally, it was observed that primers PK47F/R were able to check the Cabotiá seed hybrids of pumpkin Jabras.

关键词： Tomato Yellow Leaf Curl Virus zucchini Yellow Mosaic Virus Papaya Ringspot Virus Strain Watermelon Lettuce Mosaic Virus Jabras Hybridization

Study the Effect of Different Levels of zucchini (<i>Cucurbita pepo</i>L.) on the Biological Indicators for the Prevention of Cardiovascular Disease in Rats Fed High-Fat Diets

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Food and Nutrition Sciences 2020年第2期11卷 63-81页

作者： El-Sayeda G. E. El-Sahar Hala R. A. Sopeah Mona S. Almujaydil Home Economic Department Faculty of Specific Education Ain Shams University Cairo Egypt Food Sciences and Human Nutrition College of Agriculture and Veterinary Medicine Qassim University Al-Mulida KSA

The effects of fresh zucchini on nutritional status, and biological indicators for the prevention of cardiovascular disease in rats fed high-fat diets investigated in this study. Thirty Sprague Dawley rats were randomly divided into two main groups the first, negative control group Co (-) (n = 6), fed basal diet, The second group (n = 24) fed high-fat diet (containing basal diet + 5% tallow + 1% cholesterol + 0.02% bile salt). This group was divided into four subgroups each group 6 rats: group positive control co (+) fed high-fat diet only, group 1 (G (1)) fed high-fat diet plus 10% zucchini, group 2 (G (2) fed high-fat diet plus 15% zucchini and group 3 (G (3)) fed high-fat diet plus 20% zucchini. The levels of serum total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) were measured after eight wk. of experimental treatment. The pathologic changes of the heart, spleen and kidney were evaluated. SPSS, one way ANOVA was used to analyze the results. The results indicated that the mean values of body weight gain (BWG) and feed efficiency ratio (FIR) in G (2) and G (3) showed significant decrease compared to co (-), co (+) and G 1. The results showed that in groups (G1, G2 and G3) the relative weight of heart had significant increase when compared with control negative group. But liver and spleen weight had no significant difference when compared with control negative and positive group, the levels of TC, TG and LDL-C were significantly increased in co (+) (P zucchini (10%, 15% and 20%) had significant decrease in TC and LDL-C compared with co (+), also G2 and G3 had improve significant in HDL-C when compared with co. (+). The groups fed on zucchini give results similar to group co. (-). The best treatment was zucchini (15% and 20%) which had lowest values of total lipid cholesterol and LDL-C, and t

关键词： Atherogenic Index Cardio Protective Cucurbita pepo L. High-Fat Diet HDL-C LDL-C Lipid Profile zucchini

Required amounts of medium and fertilizer for potted culture of zucchini

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Agricultural Sciences 2012年第6期3卷 816-821页

作者： Hiromi Ikeura Takahiko Tokuda Yasuyoshi Hayata School of Agriculture Meiji University 1-1-1 Higashimita Tama-ku Kawasaki Kanagawa 214-8571 Japan

In Japan, zucchini culture has yet to get underway, and the current costs of zucchini can be attributed to damage from soil-borne disease and the unstable yields due to seasonal change of female flowers. Eradication of these problems will lead to stable supply and a consequent price reduction of zucchini fruits. We previously clarified the efficacy of potted culture as a new culture method for zucchini, but potted culture can be burdensome as the weak water and nutrient retention capacity of the medium warrants its regular replacement. To solve this problem, in this study, we investigated the blend ratio for mixing rice husk charcoal with peat and the amount of fertilizer required for potted culture of zucchini. Results revealed no significant differences in the length of the largest leaf, total number of flowers, number of female flowers, and the ratio of female flowers to total flowers with different blend ratios of rice husk charcoal to peat. However, the number of harvested fruits increased with higher ratios of rice husk charcoal to peat and was highest at 80:20. The length of the largest leaf increased with increased amounts of fertilizer, with the best response was at 200 g. No significant differences were noted between the ratio of female flowers to total flowers in any treatments. In addition, the number of harvested fruits was highest with 160 g and 200 g of fertilizer. Taken together, the optimal blend ratio of rice husk charcoal to peat is 80:20, and the optimal amount of fertilizer with this ratio is 160 g.

关键词： zucchini Rice Husk Charcoal Peat Potted Culture Fertilizer

Main Diseases of zucchini and Their Prevention and Control Methods

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Plant Diseases and Pests 2023年第1期14卷 9-13,16页

作者： Xiaowei YUAN Yimin GU Yangdong GUO Xiping SUN Xingsheng LI Huasheng Seed Group Co. Ltd.Qingzhou 262500China College of Horticulture China Agricultural UniversityBeijing 100193China

This paper elaborates the symptom characteristics and control methods of virus diseases including squash leaf curl china virus(SLCCNV),watermelon mosaic virus(WMV),cucurbit chlorotic yellows virus(CCYV),cucumber mosaic virus(CMV)and zucchini yellow mosaic virus(ZYMV),as well as fungal diseases including zucchini powdery mildew,zucchini downy mildew and zucchini scab that harm zucchini production,in order to provide a theoretical basis and technical support for disease prevention and control of zucchini.

关键词： zucchini Disease Virus disease Fungal disease Control

Direct Organogenesis from Cotyledonary Node Explants of Cucurbita pepo (L.)—An Important zucchini Type Vegetable Crop

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American Journal of Plant Sciences 2015年第1期6卷 157-162页

作者： Muruganantham Mookkan Department of Plant Pathology and Weed Science ARO Volcani Center POB6 Bet Dagan Israel Department of Horticulture University of Georgia Tifton GA USA Present Address and Correspondence: Division of Plant Sciences University of Missouri Columbia Missouri USA

Cotyledonary node explants from 3 - 5-d-old seedling derived explants of Cucurbita pepo (L.) produced multiple shoots in Murashige and Skoog (MS) salts B5 vitamins containing medium in the presence of N6-benzylamino-purine BAP 1 mg/L + Thidiazuron TDZ 0.05 mg/L. After 4 weeks explants were subcultured to medium containing MS salts B5 Vitamins BAP 0.5 mg/L, Gibberellic acid GA3 1 mg/L and L-Glutamine 15 mg/L. Periodic excision of regenerated shoots from the explants increased shoot efficiency during subculture. The combination of L-Glutamine with BA and GA3 significantly increased the shoot proliferation. Elongated shoots were excised from shoot clumps and transferred to rooting medium containing indole-3-butyric acid (IBA, 1.0 mg/L). The rooted plants were hardened in small pot containing standardized garden soil, well developed plant transferred to greenhouse and survival rate was 85%.

关键词： Cucurbita pepo zucchini Cotyledonary Node Subculture

Isolation and Identification of Squash Leaf Curl China Virus in zucchini

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Agricultural Biotechnology 2022年第5期11卷 1-3,14页

作者： Huixian SHI Yimin GU Xingsheng LI Xiping SUN Yangdong GUO Xiaowei YUAN Huasheng Seed Group Co. Ltd.Qingzhou 262500China College of Horticulture China Agricultural UniversityBeijing 100193China

[Objectives]In 2019,virus diseases occurred widely on zucchini planted in Shandong *** disease symptom was different from previous *** study aimed to identify the pathogen causing the zucchini virus disease.[Methods]Ten diseased zucchini leaves were collected in the field and used as materials for PCR and sequencing.[Results]PCR detection and sequencing showed that the nucleotide sequence of the amplified fragment had the highest identity with the squash isolate of squash leaf curl China virus(SLCCNV)(MW389919.1)in Guangdong *** were further designed for amplifying the full-length *** full-length DNA-A was 2730 bp(OM692270.1),and the full-length DNA-B was 2711 bp(OM692269.1).Through sequence alignment,it was found that the DNA-A sequence shared identity of 89.65%-99.42%with registered SLCCNV,and the identity with the SLCCNV-GDHY pumpkin isolate(MW389919.1)in Guangdong was the highest,at 99.42%.The DNA-B sequence was identical with registered SLCCNV in the range of 81.82%-97.29%,and the identity with the SLCCNV-GDHY pumpkin isolate(MW389918.1)in Guangdong,was the highest,at 97.29%.Therefore,it was speculated that SLCCNV is the pathogen of zucchini virus *** the virus was first found on zucchini in Shandong,it was named SLCCNV-SD.[Conclusions]This study provides materials for the research on the spread of SLCCNV in China and the analysis of population genetic characteristics,as well as a reference for the prevention and control of the virus in zucchini.

关键词： zucchini Viral disease Squash leaf curl China virus

Selection of Greenhouse zucchini Varieties and High-Quality,High-Yield and High-Efficiency Cultivation Techniques

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Asian Agricultural Research 2023年第6期15卷 38-40页

作者： Haijuan ZHANG Guanghui FENG Lifeng YANG Bo GENG Xiangying HOU Dongwen SUN Zibo Digital Agriculture and Rural Development Center Zibo 255000China Shandong Agriculture and Engineering University Zibo 255300China

[Objectives]To select zucchini varieties suitable for cultivation in Zibo City and test its high-yield cultivation techniques.[Methods]Six zucchini varieties were introduced,and their commercial quality and yield were determined.[Results]The yield of Shengfeier,Xiuyu 170 and Xihulu 309 increased by 11.4%,6.9%and 4.6%,respectively compared with S68(control),and zucchini was straight,looked pleasing to the eye,and had strong disease resistance.[Conclusions]The zucchini varieties were selected and the high-quality,high-yield and high-efficiency cultivation techniques were integrated.

关键词： zucchini Variety High-quality high-yield and high-efficiency Cultivation techniques

Identify Plant Drought Stress by 3D-Based Image

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Journal of Integrative Agriculture 2012年第7期11卷 1207-1211页

作者： ZHAO Yan-dong SUN Yu-rui CAI Xiang LIU He Peter Schulze Lammers School of Technology Beijing Forestry University Beijing 100083 P.R.China College of Information and Electrical Engineering China Agricultural University Beijing 100083 P.R.China Department of Agricultural Engineering University of Bonn Bonn 53115 Germany

Plants respond to drought stress with different physical manners, such as morphology and color of leaves. Thus, plants can be considered as a sort of living-sensors for monitoring dynamic of soil water content or the stored water in plant body. Because of difficulty to identify the early wilting symptom of plants from the results in 2D （two-dimension） space, this paper presented a preliminary study with 3D （three-dimension）-based image, in which a laser scanner was used for achieving the morphological information of zucchini （Cucurbita pepo） leaves. Moreover, a leaf wilting index （DLWIF） was defined by fractal dimension. The experiment consisted of phase-1 for observing the temporal variation of DLWIF and phase-2 for the validation of this index. During the experiment, air temperature, luminous intensity, and volumetric soil water contents （VSWC） were simultaneously recorded over time. The results of both phases fitted the bisector （line： 1：1） with R2=0.903 and REMS=0.155. More significantly, the influence of VSWC with three levels （0.22, 0.30, and 0.36 cm3 cm-3） on the response of plant samples to drought stress was observed from separated traces of DLWIF. In brief, two conclusions have been made：（i） the laser scanner is an effective tool for the non-contact detection of morphological wilting of plants, and （ii） defined DLWIF can be a promising indicator for a category of plants like zucchini.

关键词： wilting 3D-based image laser scanner fractal dimension drought stress zucchini