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Up-regulation of a homeodomain-leucine zipper gene HD-1 contributes to trichome initiation and development in cotton

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Journal of Integrative Agriculture 2019年第2期18卷 361-371页

作者： NIU Er-li CAI Cai-ping BAO Jiang-hao WU Shuang ZHAO Liang GUO Wang-zhen State Key Laboratory of Crop Genetics&Germplasm Enhancement Ministry of Science and Technology/Hybrid Cotton R&D Engineering Research CenterMinistry of Education/College of AgricultureNanjing Agricultural UniversityNanjing 210095P.R.China

Plant trichomes originate from epidermal *** this work,we demonstrated that a homeodomain-leucine zipper(HD-Zip)gene,Gh_A06G1283(Gh HD-1A),was related to the leaf trichome trait in allotetraploid cotton and could be a candidate gene for the T_1 *** ortholog of GhHD-1A in the hairless accession Gossypium barbadense ***7124 was interrupted by a long terminal repeat(LTR)retrotransposon,while GhHD-1A worked well in the hairy accession Gossypium hirsutum *** and phylogenetic analysis showed that GhHD-1A belonged to the HD-Zip IV gene family,which mainly regulated epidermis hair development in *** of GhHD-1A and its homoeologs GhHD-1D in allotetraploid T586and Hai7124 could significantly reduce the density of leaf hairs and affect the expression levels of other genes related to leaf trichome *** analysis found that GhHD-1A mainly regulated trichome initiation on the upper epidermal hairs of leaves in cotton,while the up-regulated expression of GhHD-1A in different organs/tissues also altered epidermal trichome *** study not only helps to unravel the important roles of GhHD-1A in regulating trichome initiation in cotton,but also provides a reference for exploring the different forms of trichome development in plants.

关键词： leaf trichome map-based cloning a homeodomain-leucine zipper gene HD-1 virus-induced gene silencing (VIGS) functional differentiation

45nm低功耗、高性能zipper CMOS多米诺全加器设计

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电子学报 2009年第2期37卷 266-271页

作者：汪金辉宫娜耿淑琴侯立刚吴武臣董利民北京工业大学集成电路与系统研究室北京100022 河北大学电子信息工程学院河北保定071002

提出了电荷自补偿技术,此技术利用P型多米诺电路动态结点的放电对N型多米诺电路的动态结点充电,并在此技术基础上综合应用双阈值技术和多电源电压技术,设计了新型低功耗、高性能zipper CMOS多米诺全加器.仿真过程中提出了功耗分布法,精... 详细信息

提出了电荷自补偿技术,此技术利用P型多米诺电路动态结点的放电对N型多米诺电路的动态结点充电,并在此技术基础上综合应用双阈值技术和多电源电压技术,设计了新型低功耗、高性能zipper CMOS多米诺全加器.仿真过程中提出了功耗分布法,精确找到了电荷自补偿技术的最优路径.仿真结果表明,在相同的时间延迟下,与标准zipper CMOS多米诺全加器、双阈值zipper CMOS多米诺全加器、多电源电压zipper CMOS多米诺全加器相比,新型zipper CMOS多米诺全加器动态功耗分别减小了37%、35%和7%,静态功耗分别减小了41%,20%和43%.最后,分析并得到了新型全加器漏电流最低的输入矢量和时钟状态.

关键词：动态功耗静态功耗漏电流 zipper CMOS多米诺全加器电荷自补偿技术

Homeobox leucine zipper proteins and cotton improvement

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Advances in Bioscience and Biotechnology 2013年第10期4卷 15-20页

作者： Muzna Zahur Muhammad Ahsan Asif Nadia Zeeshan Sajid Mehmood Muhammad Faheem Malik Abdul R. Asif Department of Biochemistry and Molecular Biology University of Gujrat Gujrat Pakistan Department of Clinical Chemistry University Medical Center Goettingen Goettingen Germany

Transcription factors play key roles in plant development and stress responses through their interaction with cis-elements and/or other transcription factors. Homeodomain associated leucine zipper proteins (HD-Zip) constitute a family of transcription factors that are characterized by the presence of a DNA-binding domain closely linked with leucine zipper motif functioning in dimer formation. This type of association is unique to plants and considered as an excellent candidate to activate developmental responses to altering environmental conditions. Cotton is the most important fiber plant with a lot of local and commercial uses in the world. HD-Zip proteins not only have key roles in different stages of vascular and inter-fascicular fiber differentiation of cotton but also are suggested to have an important role against abiotic stress that is one of the key factors limiting cotton productivity. Plants have developed various strategies to manage stress conditions through a combination of metabolic, physiological and morphological adaptations. These adaptive changes rely largely on alterations in gene expression. Therefore, transcriptional regulators play a crucial role in stress tolerance. Being a transcription factor HD-Zip might be a useful target for genetic engineering to generate multiple stress tolerance in susceptible plants. In the following chapter, we discussed how the HD-Zip proteins would play a useful role for cotton development both in fiber production and stress adaptation.

关键词： Cotton Stress Transcription Factor HD-Zip Proteins Homeobox Leucine zipper

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Velcro & zipper

数理天地（初中版） 2019年第1期 48-49页

One day,a Swiss inventor named George de Mestral was walking in the ***,he saw that many burrs had stuck to his wool *** wondered why they were hard to pull off. He looked at the burrs under a microscope,and saw that ... 详细信息

关键词： Velcro zipper

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zipper精细优化设计

Zipper精细优化设计

2020年中国地球科学联合学术年会

作者：魏国伟郭善力白杰陈永胜东方地球物理公司国际勘探事业部

近年来,高密度、宽方位三维地震高效采集越来越得到油公司的青睐,采集道数得到了十几甚至几十倍增长,道数的增加带来了排列故障时间大幅攀升,设备数量增加和有效产量的降低导致地震队的作业成本不断升高;zipper作业技术,一方面解决了整... 详细信息

近年来,高密度、宽方位三维地震高效采集越来越得到油公司的青睐,采集道数得到了十几甚至几十倍增长,道数的增加带来了排列故障时间大幅攀升,设备数量增加和有效产量的降低导致地震队的作业成本不断升高;zipper作业技术,一方面解决了整个区块采集设备数量不足的问题,同时可以有效降低超大排列带来的故障时间,所以在现在的三维勘探中得到了充分的应用;在zipper作业设计时,在现有激发和接收设备的前提下,还需要进行精细优化设计,实现激发和接收进度的匹配,达到最高的生产产量和效益的最大化。本文将从炮道比、最少炮设计及最优化接收等方面出发对zipper设计精细优化展开讨论。

关键词：检波点互换法 zipper 高效采集优化设计

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zipper技术在三维地震采集中的应用

Zipper技术在三维地震采集中的应用

你的内存还够用吗——用Memory zipper Plus压缩优化内存

中国石油学会2019年物探技术研讨会

作者：肖虎李红远赵君杨国平白志宏东方地球物理公司采集技术中心

三维地震采集的施工方式一般分为两种:排列纵向滚动或排列横向滚动。当接收设备投入数量有限,导致纵向滚动或完全横向滚动均无法实现,可以采取zipper技术施工。zipper技术类似衣服的拉链作用,把工区沿检波线方向分成若干块,分块依次横... 详细信息

三维地震采集的施工方式一般分为两种:排列纵向滚动或排列横向滚动。当接收设备投入数量有限,导致纵向滚动或完全横向滚动均无法实现,可以采取zipper技术施工。zipper技术类似衣服的拉链作用,把工区沿检波线方向分成若干块,分块依次横向滚动施工,最终把所有块的数据合并在一起;在zipper交界处重复一定的炮点或接收排列,确保合并后数据的完整。该方法虽然重复了一定的炮点或检波点,但由于利用了横向滚动道炮比低的优势,既保证了生产效率,又弥补了设备的不足。

关键词：纵向滚动横向滚动道炮比 zipper

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电脑技术——Hello-IT 2002年第12期 39-41页

作者：王光临

现在虽说大家的内存普遍都是128M、256M的水平了,但是系统和应用程序对内存的需求似乎永远没有止境,Win XP系统就是一个吃内存的大户,没有256M的内存很难跑得顺畅,即使有256M内存,如果不合理应用的话,也会时常遇到剩余内存不足等问题,... 详细信息

现在虽说大家的内存普遍都是128M、256M的水平了,但是系统和应用程序对内存的需求似乎永远没有止境,Win XP系统就是一个吃内存的大户,没有256M的内存很难跑得顺畅,即使有256M内存,如果不合理应用的话,也会时常遇到剩余内存不足等问题,怎么会遇到这种情况呢?这主要是由于许多应用程序的内存管理机制不是很完善,往往在程序结束后还占据着部分内存,致使系统无法将这些内存及时收回再利用,从而导致内存不足。解决这个问题的办法就是将这些内存及时收回,Memory zipper Plus就可以帮我们轻松地回收剩余的内存,并且还可以帮我们为CPU降温、管理程序进程、保护数据。

关键词： Memory zipper Plus 压缩优化内存内存优化软件虚拟内存系统内存内存管理

cDNA cloning and function analysis of two novel erythroid differentiation related genes

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Science China(Life Sciences) 2001年第1期44卷 99-105页

作者：王鑫王敦成陈兴胡美茹王建安黎燕郭宁沈倍奋 1. Beijing Institute of Basic Medical Sciences 100850 Beijing China

Our previous studies showed that some nuclear proteins that were expressed especially during terminal differentiation of erythroid cells might interact directly or indirectly with HS2 sequence to form the HS2-protein complexes and thus play an important role in the globin gene regulation and erythroid differentiation. Monoclonal antibodies against the nuclear proteins of terminal differentiated erythroid cells, including intermediate and late erythroblasts of human fetal liver and hemin induced K562 cells, were prepared by hybridoma technique. The monoclonal antibodies were used to screen λ-gtll human cDNA expression library of fetal liver in order to obtain the relevant cDNA clones. By the analysis of their cDNA clones and the identification of the proteins' functions, the regulation mechanism of the HS2 binding proteins might be better understood. Two cDNA clones (GenBank accession number AF040247 and AF040248 respectively) were obtained and one of them owns a full length and the other encodes a protein characterized by a leu-cine-zipper domain. Both of them were expressed differentially in K562 cells and hemin-induced K562 cells. The evidence suggested that both of them were involved in erythroid differentiation. We investigated the expression pattern of EDRF1 and EDRF2 by RT-PCR technique. The results of RT-PCR suggested that EDRF1 and EDRF2 might play a critical role in early stage of organ development and histological differentiation. EDRF1 and EDRF2 might start the program of erythroid development, and also regulate the development of erythroid tissue and the expression of globin gene at different stage of the development.

关键词： erythroid differentiation, HS2, cDNA cloning, leucine zipper domain, RT-PCR.

A novel erythroid differentiation related gene EDRF2 inhibited α-globin gene expression in K562 cells

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Chinese Science Bulletin 2002年第5期47卷 398-402页

作者： WANG Duncheng, YANG Xiaoming & SHEN BeifenInstitute of Basic Medical Sciences, Institute of Radiation Medicine Sciences, Academy of Military Sciences, Beijing 100850, China Institute of Basic Medical Sciences Institute of Radiation Medicine Sciences Academy of Military Sciences Beijing China

In previous studies, we found that EDRF2 was an erythroid differentiation related factor, whose expression was markedly upregulated during erythroid differentiation. It suggested that this factor played a role in erythropoiesis. By using rapid amplification of cDNA ends technology, we cloned EDRF2 gene 5’-and 3’-cDNA ends successfully. Transfection and Northern blot analysis demonstrated that EDRF2 inhibited mRNA expression of a-globin gene, while did not regulate γ-globin gene expression. Gel shift assay confirmed that DNA-binding activity of erythroid specific transcription factors GATA-1, NF-E2 and AP1 was not affected by either forced overexpression or artificial down-regulation of EDRF2 gene in K562 cells. However, we detected that the negative regulator of expression of GATA-1 transcription factor was increased in EDRF2 overexpressed K562 cells. These results strongly suggested that EDRF2 was involved in a-globin gene expression and erythroid differentiation and served as a negative regulator of

关键词： globin gene erythroid specific transcription factors leucine zipper RACE EMSA.