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A Preliminary Study on Anti-Colorectal Cancer Effect and Molecular Mechanism of Aegiceras Corniculatum Extract

World Journal of Traditional Chinese Medicine 2023年第4期9卷 404-414页

作者： De‑Chao Tan Xiao‑Tao Hou Hua Luo Yi‑Wei Chen Zheng‑Cai Du Jin‑Ling Xie Lin‑Yao Wei Chi-Teng Vong Xiao‑Yan Wen Er‑Wei Hao Jia‑Gang Deng Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica Guangxi University of Chinese MedicineNanningChina Guangxi Collaborative Innovation Center of Research on Functional Ingredients of Agricultural Residues NanningChina Institute of Chinese Medical Sciences State Key Laboratory of Quality Research in Chinese MedicineUniversity of MacaoMacaoChina Sino‑Canada Joint zebrafish Lab for Chinese Herbal Drug Screening Guangxi University of Chinese MedicineNanningChina zebrafish Center for Advanced Drug Discovery Keenan Research Center for Biomedical ScienceLi Ka Shing Knowledge InstituteSt.Michael’s HospitalTorontoOntarioCanada

Objective:To study the inhibitory effects on colorectal cancer(CRC)and the underlying mechanism of the petroleum ether extract of Aegiceras corniculatum leaves(PACL).Materials and Methods:The effect of PACL on the proliferation of CRC cell lines DLD-1,HT-29,and SW480was measured by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay and colony-forming *** then,a wound-healing assay was used to measure the migration ability of three CRC *** cell cycle and apoptosis of three CRC cells were measured by PI/RNase staining and annexin V-FITC/double staining,respectively,and the intrinsic apoptosis pathway was studied by the Western *** anti-CRC effect of PACL in vivo was evaluated by HT-29 xenograft zebrafish ***:PACL inhibited cell viability and proliferation in DLD-1,HT-29,and SW480 cells in a dose-and time-dependent *** can inhibit cell migration in DLD-1and SW480 cells but not in the less mobile phenotype cell *** treatment resulted in cell cycle arrest of DLD-1 and HT-29 cells in the G2/M ***,PACL can induce apoptosis in all three CRC cells,which may be achieved by regulating the intrinsic apoptosis pathway mediated by mitochondria and the endoplasmic ***,the tumor sizes were decreased after treatment with PACL and PACL combined with fluorouracil in HT-29 xenograft zebrafish ***:These findings suggested that PACL may exert its anti-CRC effect by inducing apoptosis through the intrinsic apoptosis pathway and show a significant anti-CRC effect in vitro and in vivo,so it might be potentially developed as an anti-CRC agent.

关键词： Aegiceras corniculatum apoptosis cell cycle colorectal cancer zebrafish xenograft

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