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PagGRF12a interacts with PagGIF1b to regulate secondary xylem development through modulating PagXND1a expression in Populus alba×***

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Journal of Integrative Plant Biology 2021年第10期63卷 1683-1694页

作者： Jinnan Wang Houjun Zhou Yanqiu Zhao Cheng Jiang Jihong Li Fang Tang Yingli Liu Shutang Zhao Jianjun Hu Xueqin Song MengZhu Lu State Key Laboratory of Tree Genetics and Breeding Key Laboratory of Tree Breeding and Cultivation of the State ForestryAdministrationResearch Institute of ForestryChinese Academy of ForestryBeijing 100091China Key Laboratory of Agricultural Ecology and Environment College of ForestryShandong Agricultural UniversityTai'an 271018China School of Agriculture Ludong UniversityYantai 264025China State Key Laboratory of Subtropical Silviculture School of Forestry and BiotechnologyZhejiang Agriculture&Forestry UniversityHangzhou 311300China CoInnovation Center for Sustainable Forestry in Southern China Nanjing Forestry UniversityNanjing 210037China

Growth-regulating factors(GRFs)are important regulators of plant development and growth,but their possible roles in xylem development in woody plants remain ***,we report that Populus alba×Papulus glandulosa PagGRF12a negatively regulates xylem development in ***12a is expressed in vascular *** to non-transgenic control plants,transgenic poplar plants overexpressing PagGRF12a exhibited reduced xylem width and plants with repressed expression of PagGRF12a exhibited increased xylem *** NAC domain 1(XND1)encodes a NAC domain transcription factor that regulates xylem development and transcriptional analyses revealed that PagXND1a is highly upregulated in PagGRF12a-overexpressing plants and downregulated in PagGRF12a-suppressed plants,indicating that PagGRF12a may regulate xylem development through *** transcriptional assays and chromatin immunoprecipitation-polymerase chain reaction assays confirmed that PagGRF12a directly upregulates *** addition,PagGRF12a interacts with the GRF-Interacting Factor(GIF)PagGIF1b,and this interaction enhances the effects of PagGRF12a on *** results indicate that PagGRF12a inhibits xylem development by upregulating the expression of PagXND1a.

关键词： GRF GIF1 Populus wood formation XND1 xylem development

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Perturbation of Wood Cellulose Synthesis Causes Pleiotropic Effects in Transgenic Aspen

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Molecular Plant 2011年第2期4卷 331-345页

作者： Chandrashekhar R Joshi Shivegowda Thammannagowda Takeshi Fujino Ji-Qing Gou Utku Avci Candace H. Haigler Lisa M. McDonnell Shawn D. Mansfield Bemnet Mengesha Nicholas C. Carpita Darby Harris Seth DeBolt Gary F. Peter Biotechnology Research Center School of Forest Resources and Environmental Sciences Michigan Technological University Houghton MI 49931 USA Department-of Bioenergy Science and Technology Chonnam National University Buk-Gu Gwangju 500-757 Korea Department of Crop Science North Carolina State University 4405 Williams Hall Raleigh NC 27695 USA Department of Plant Biology North Carolina State University 4405 Williams Hall Raleigh NC 27695 USA Department of Wood Science University of British Columbia 4030-2424 Main Mall Vancouver BC V6T 1Z4 Canada Purdue University Botany and Plant Pathology 915 West State Street West Lafayette IN 47907 USA Department of Horticulture N-318 Agricultural Sciences Center University of Kentucky Lexington KY 40546 USA School of Forest Resources and Conservation 326 Newins-Ziegler Hall PO Box 110410 University of Florida Gainesville FL 32611 USA

Genetic manipulation of cellulose biosynthesis in trees may provide novel insights into the growth and development of trees. To explore this possibility, the overexpression of an aspen secondary wall-associated cellulose synthase （PtdCesAS） gene was attempted in transgenic aspen （Populus tremuloides L.） and unexpectedly resulted in silencing of the transgene as well as its endogenous counterparts. The main axis of the transgenic aspen plants quickly stopped growing, and weak branches adopted a weeping growth habit. Furthermore, transgenic plants initially developed smaller leaves and a less extensive root system. Secondary xylem （wood） of transgenic aspen plants contained as little as 10% cellulose normalized to dry weight compared to 41% cellulose typically found in normal aspen wood. This massive reduction in cellulose was accompanied by proportional increases in lignin （35%） and non-cellulosic polysaccharides （55%） compared to the 22% lignin and 36% non-cellulosic polysaccharides in control plants. The transgenic stems pro- duced typical collapsed or ＇irregular＇ xylem vessels that had altered secondary wall morphology and contained greatly reduced amounts of crystalline cellulose. These results demonstrate the fundamental role of secondary wall cellulose within the secondary xylem in maintaining the strength and structural integrity required to establish the vertical growth habit in trees.

关键词： Aspen cellulose synthesis transgenic trees xylem development cell wall lignin irregular xylem growth crystallinity.

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PagERF81 regulates lignin biosynthesis and xylem cell differentiation in poplar

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Journal of Integrative Plant Biology 2023年第5期65卷 1134-1146页

作者： Xin-Wei Zhao Qiao Wang Dian Wang Wei Guo Meng-Xuan Hu Ying-Li Liu Gong-Ke Zhou Guo-Hua Chai Shu-Tang Zhao Meng-Zhu Lu State Key Laboratory of Tree Genetics and Breeding Research Institute of ForestryChinese Academy of ForestryBeijing 100091China Collegeof Resources and Environment QingdaoAgricultural UniversityQingdao 266109China CollegeofAgronomy QingdaoAgriculturalUniversityQingdao 266109China TaishanAcademyof Forestry Sciences Taian271000China College of Landscape Architecture and Forestry Qingdao Agricultural UniversityQingdao 266109China State Key Laboratory of Subtropical Silviculture College of Forestry and BiotechnologyZhejiang A&F UniversityHangzhou 311300China

Lignin is a major component of plant cell walls and is essential for plant growth and development. Lignin biosynthesis is controlled by a hierarchical regulatory network involving multiple transcription factors. In this study, we showed that the gene encoding an APETALA 2/ethylene-responsive element binding factor(AP2/ERF) transcription factor, PagERF81,from poplar 84 K(Populus alba × P. glandulosa) is highly expressed in expanding secondary xylem cells. Two independent homozygous Pagerf81 mutant lines created by gene editing, produced significantly more but smaller vessel cells and longer fiber cells with more lignin in cell walls, while PagERF81 overexpression lines had less lignin,compared to non-transgenic controls. Transcriptome and reverse transcription quantitative PCR data revealed that multiple lignin biosynthesis genes including Cinnamoyl CoA reductase 1(PagCCR1),Cinnamyl alcohol dehydrogenase 6(PagCAD6), and 4-Coumarate-CoA ligase-like 9(Pag4CLL9) were upregulated in Pagerf81 mutants, but down-regulated in PagERF81 overexpression lines. In addition, a transient transactivation assay revealed that PagERF81 repressed the transcription of these three ***, yeast one hybrid and electrophoretic mobility shift assays showed that PagERF81 directly bound to a GCC sequence in the PagCCR1 promoter. No known vessel or fiber cell differentiation related genes were differentially expressed, so the smaller vessel cells and longer fiber cells observed in the Pagerf81 lines might be caused by abnormal lignin deposition in the secondary cell walls. This study provides insight into the regulation of lignin biosynthesis, and a molecular tool to engineer wood with high lignin content, which would contribute to the lignin-related chemical industry and carbon sequestration.

关键词： lignin biosynthesis PagERF81 Populus secondary cell wall xylem development

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