检索结果-南通市图书馆

Agricultural Sciences in China 2003年第2期2卷 206-213,T005页

作者： XUZi－rong LUJian－jun KeyLaboratoryofAnimalMolecularNutrition MinistryofEducation／FeedScienceInstituteZhejiangUniversityHangzhou310029P.R.China KeyLaboratoryofAnimalMolecularNutrition MinistryofEducation／FeedScienceInst

Ninety Landrace×Jia 35±0. 40 kg weight growing pigs were randomly allotted to three treatments , each of which was replicated three times with ten pigs per replicate. The pigs were reared on either a conventional corn-based diet (control I ) or a paddy-based diet (control I ) or a paddy diet supplemented with 0.2% NSP enzymes (test group). All pigs were given ad libitum access to both feed and water. The results of feeding trial showed that supplementation of NSP enzymes significantly increased ADG by 8.78% (P0. 05). The lengths of the villi within the duodenal, jejunal and ileal sections of the small intestine of pigs receiving the NSP enzymes diet were increased by 23. 68 (P<0. 05), 56. 00 (P<0. 01) and 76. 90%(P<0. 01) respectively, relative to the pigs in controlⅡ.

关键词： Growing pig NSP enzymes xylanase β-glucanase Cellulase Digestive tract

维普期刊数据库

Volume Change of the Random Coil to Folded Conformational Transition of Thermomyceslanuginosusxylanase at 24&#176C and pH = 7.0 via Application of the Clausius-Clapeyron Equation

在线全文

学校读者我要写书评

暂无评论

Journal of Biophysical Chemistry 2014年第4期5卷 134-142页

作者： Heather N. H. Wilks Tara M. Arrington Billy Mark Britt Department of Chemistry and Biochemistry Texas Woman’s University Denton Texas USA

A partial phase diagram characterizing the conformational change that occurs in Thermomyces lanuginosus xylanase as it is slowly heated in 150 mM sodium phosphate (pH = 7.0) has been con-structed from slow-scan-rate differential scanning calorimetry measurements. The Clausius-Clapeyron equation was applied to determine an associated volume change of -205 L·mol-1 at 24°C, the equilibrium transition temperature at 1.0 atm pressure. This value is in excellent agreement with that predicted using a previously published [1] empirical equation for calculating the hydro-dynamic radius if the transition is regarded as from a random coil to a functional, folded state and with the assumption that the hydrodynamic radius is a good approximation of the true random coil radius. The existence of a low-temperature random coil is confirmed by circular dichroism and dynamic light scattering measurements. Thus, at 24°C and 1.0 atm pressure the enzyme appears to fold from a random coil to a functional, folded form as it is slowly heated.

关键词： Clausius-Clapeyron Conformational Change Slow-Scan-Rate Differential Scanning Calorimetry Thermomyces lanuginosus xylanase Volume Change

xylanase PREBLEACHING ON NaOH-AQ WHEAT STRAW PULP

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

天津科技大学学报 2004年第S2期 272-276页

作者： Caixia Li1, Yongjun Deng1, Ping Li1,Guigan Fang1, Shuchai Liu2(1 Research Institute of Chemical Processing and Utilization of Forest Products, CAF, Nanjing, 21004 2 Northwest Institute of Light Industry, Light Chemical Industry Department, Xianyang, 712081)

Before calcium hypochlorite bleaching (H) and chlorination,alkaline extraction, calcium hypochlorite three-stage-bleaching (CEH),we used a kind of hemicellulase, xylanase, to treat wheat straw pulp from Gaoyou Papermill. xylanase pretreatment contained tow stages, the first stage was xylanase treatment, which was followed by alkaline extraction, the second stage. The xylanase could act on partial lignin and carbohydrate, chiefly xylan. The following alkaline extraction could dissolve something that could not be removed during the first stage. The result of pretreatment was to facilitate penetration of bleaching chemicals, to reduce effective chlorine consumption and to lower pollution loading of bleaching effluent. In the case of these two bleaching processes, the enzymatic pretreatment substantially enhanced the optical properties of the pulps. To calcium hypochlorite bleaching, strength properties of pulps were improved.

关键词： xylanase wheat straw pulp H bleaching CEH bleaching.

Mutagenesis and Screening of High Yield xylanase Production Strain of Aspergillus usamii by Microwave Irradiation

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Chemical Engineering 2003年第5期11卷 594-597页

作者：李永泉陈时飞岑沛霖 College of Life Science College of Life Science Food and Drug Adiministration of Zhejiang Province Hangzhou 310012 China Institute of Bioengineering Zhejiang University Hangzhou 310027 China

A high yield xylanase producing strain, A. usamii L336-23, was screened out from its parent strain *** L336 after microwave irradiation. Its productivity of xylanase activity increased by 35.7% from 21000μu·ml-1 to 28500μu·ml-1 and was stable after frequent subcultures and storage for more than two months. The mechanism of microwave mutation was also discussed.

关键词： microwave irradiation Aspergilles usamii xylanase

维普期刊数据库

Production of xylanase and β-Xylosidase Enzymes by Pseudozyma hubeiensisin Solid State Fermentation

在线全文

学校读者我要写书评

暂无评论

Advances in Microbiology 2019年第5期9卷 467-478页

作者： Nutan Mhetras Vidhyashri Mapare Digambar Gokhale Symbiosis School of Biological Sciences Symbiosis International University Lawale Pune India NCIM Resource Center CSIR-National Chemical Laboratory Pune Maharashtra India

xylanase is an important enzyme with potential application in the degradation of xylan component in the lignocellulosic biomass. There are very few reports on the production of cellulase free xylanases especially by yeast strains which have great potential in paper and pulp industry in removing the hemicellulose from the treated or untreated pulp. In this study, P. hubeiensis NCIM 3574 isolated in our laboratory produced significant levels of extracellular cellulase free xylanase (2480 IU/g DSS) in solid state fermentation (SSF) using wheat bran and xylan. It also produced high levels of β-xylosidase (198 IU/g DSS) when grown in SSF using ground nut oil cake and xylan. These highest activities were obtained when fermented Koji was extracted with 1% NaCl supplemented with 0.5% of Triton X-100. These are the highest activities reported so far from yeast strains in the available literature. The crude xylanase preparation of P. hubeiensis produced xylooligosaccharides (XOS) without xylose proving its potential for XOS production with no further requirement of downstream processing. The XOS as prebiotic show beneficial effect on gut microflora such as Lactobacilli and Bifidobacteria which suppress the activity of pathogenic organisms. This xylanase also has a potential application as a bio-bleaching agent in paper and pulp industry.

关键词： Pseudozyma hubeiensis Cellulase-Free xylanase β-Xylosidase Solid State Fermentation Submerged Fermentation

Effects of xylanase and Protease Supplementation on Nutrient Digestibility in Diets Based on Peas and Wheat Fed to Weaned Pigs

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2011年第1期2卷 19-26页

作者： L.Hargreaves W.C.Sauer Department of Agricultural Food and Nutritional ScienceUniversity of Alberta

This experiment was conducted to determine the effects of a commercial enzyme mixture providing xylanase and protease activities on the digestibility of dietary nutrients when added to diets based on peas and wheat and fed to 9. 5 to 15 kg pigs. Pigs were weaned at 18 to 21 days of age and fitted with a simple T-cannula at the distal ileum,6 to 7 days after weaning. After 5 to 7 days for adaptation to their diets, ileal digesta, and feces were collected. Enzyme supplementation had no effect on the apparent ileal or fecal digestibility of any dietary nutrient. There was a numerical improvement （ P 〉 0.05 ） in the ileal digestibility of fiber. In conclusion, xylanase and protease appeared to be ineffective in improving nutrient digestibility when supplemented in diets based on peas and wheat fed to weaned pigs.

关键词： nutrient digestibility protease weaned pigs xylanase

维普期刊数据库

Purification and Characterization of Thermostable Cellulase Free xylanase from PseudomonasSp. XPB-6

在线全文

学校读者我要写书评

暂无评论

Advances in Microbiology 2012年第1期2卷 17-25页

作者： Pawan Kumar Sharma Duni Chand Department of Biotechnology Himachal Pradesh University Shimla India

Extracellular cellulase free xylanase from Pseudomonas sp. XPB-6 was purified to homogeneity with 2.15 fold of purity and 60.31% yield, respectively by ammonium sulfate precipitation and DEAE ion exchange chromatography. The protein was found to be a homotrimer consisting of three subunits of 41 kDa each and the native molecular weight of 123 kDa. The pH and temperature optimum for enzyme were 7.5 in 100 mM sodium phosphate buffer at 60°C and was stable to a high and broader range of pH and to a temperature up to 75°C. Kinetic experiments at 60°C gave Vmax and Km values of 144.92 U/mg and 0.60 mg·ml-1 respectively for birch wood xylan. The enzyme had no apparent requirement for cofactors, and its activity was strongly inhibited 1 mM Hg2+ and Cu2+ while it was slightly inactivated by 1 mM Mg2+ and Pb2+. Shelf life of xylanase of Pseudomonas sp. XPB-6 was studied both at room temperature and 4°C. The enzyme was stable both at room temperature and at 4°C for 90 and 28 days respectively. The half-life of the enzyme was approximately 40 days at room temperature.

关键词： xylanase Pseudomonas sp. XPB-6 Purification DEAE Chromatography

Bioinformatics Analysis of xylanase xynMF13-GH10 Gene from Mangrove Fungi

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Agricultural Research 2022年第5期14卷 36-40,50页

作者： Junxia SONG Wanting WU Hongbing QI School of Geographic Sciences Lingnan Normal University Zhanjiang 524048 China Life Science & Technology School Lingnan Normal University Zhanjiang 524048 China

[Objectives]The paper was to analyze and predict the structure and characteristics of xylanase xynMF13-GH10 gene and its encoded protein.[Methods]xynMF13-GH10 gene was predicted by NCBI and various information analysis tools in ExPASy website,as well as SignaIP 5.0,DNAman,TMHMM,SOPMA and SWISS-Model,and the characteristics and functions of protein structure encoded by the gene were predicted.[Results]The gene is 1332 bp in length,the coding region is 1-1332 bp,and the gene encodes 443 amino *** xynMF13-GH10 gene has high homology with xylanase in many species,and it has the highest homology with Paraphaeosphaeria sporulosa endoxylanase-like protein,with the consistency reaching 78.91%and e-value reaching *** secondary structure consists of 48.31%random curl,30.25%α-helix structure,16.70%extended chain and 4.74%β-corner.[Conclusions]The results provide a reference for revealing the physiological function and expression regulation mechanism of xynMF13-GH10 gene in the future.

关键词： xylanase Mangrove xylanase gene Bioinformatics

Production of Cellulase and xylanase by Aspergillus terreus KJ829487 Using Cassava Peels as Subtrates

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advances in Microbiology 2016年第7期6卷 502-511页

作者： Afolake Atinuke Olanbiwoninu Sunday Ayodele Odunfa Department of Biological Sciences Faculty of Natural Sciences Ajayi Crowther University Oyo Nigeria Department of Microbiology Faculty of Sciences University of Ibadan Ibadan Nigeria

Cassava (Manihot esculenta, Crantz) is one of the most important food plants in West Africa. Its peels are made up of cellulose, hemicellulose and lignin. This lignocellulolytic biomass can be converted using microbial enzymes to fermentable sugars which have wide range of biotechnological relevance in many fermentation processes. The aim of this study is to screen filamentous fungi from decaying cassava peels that are good producers of xylanases and cellulases. Decaying parts of cassava peels were obtained and brought to the laboratory for further work. Fungi were isolated, identified and screened for cellulase and xylanase production. Isolate with highest frequency of occurrence and enzyme production was identified using phenotypic and molecular method. Optimisation of growth conditions for enzymes production was monitored using the DNSA method, also saccharification of cassava peel were carried out using the enzymes obtained from the isolate. Aspergillus terreus KJ829487 was the predominant fungus. It produces cellulases and xylanases optimally at 40°C, pH 6 and 8, utilising carboxymethylcellulose (CMC) or xylose and yeast extracts as its carbon and nitrogen sources respectively. Saccharification of the peels yielded 584 mg/L glucose, 78 mg/L xylose and 66 mg/L rhamnose. Aspergillus terreus KJ829487 obtained from cassava peels have the ability to produce high concentration cellulases and xylanases which effectively hydrolysed the lignocelluloses’ biomass to fermentable sugars.

关键词： Cassava Peels Aspergillus terreus Cellulase xylanase Lignocelluloses Fermentable Sugars

Comparable Study of xylanase Production from Aspergillus niger via Solid State Culture

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Chemistry and Chemical Engineering 2012年第12期6卷 1106-1113页

作者： Norazhna I Pushpahvalli B Ku Halim K.H Norakma M.N Faculty of Engineering Universiti Selangor Bestari Jaya 45600 Malaysia Faculty of Science and Biotechnology Universiti Selangor Bestari daya 45600 Malaysia Faculty of Chemical Engineering Universiti Teknologi Mara Selangor 40450 Malaysia

With the high availability of agricultural wastes in Malaysia, green energy is practical to be conducted. Thus, a research on the modification of the physical conditions of the solid state culture system, specifically different initial moisture content, fermentation temperature, inoculums size and different percentage of carbon and nitrogen sources were conducted to compare the usage of two different lignocellulosic materials which were oil palm frond and banana petiole respectively. After using a conventional method （one factor at a time）, the optimum fermentation conditions would be a combination of 70% initial moisture content （v/w）, at 28 ~C fermentation temperature with supplementation of 1% sucrose （w/w） and 1% peptone using 1 mL of inoculum in order to achieve highest xylanase production which was 1,711.87 U/mL and 1,579.60 U/mL for oil palm frond and banana petiole respectively. It was suggested that oil palm frond showed higher xylanase production compared to banana petiole.

关键词： xylanase solid state culture Aspergillus niger oil palm frond banana petiole.