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xylanase improves the intestinal barrier function of Nile tilapia(Oreochromis niloticus)fed with soybean(Glycine max)meal

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畜牧与生物技术杂志:英文版 2023年第5期14卷 2224-2238页

作者： Tong Wang Nannan Zhou Junyi He Zhenzhen Hao Chentao Zhou Yidi Du Zhenyu Du Xiaoyun Su Meiling Zhang Laboratory of Aquaculture Nutrition and Environmental Health(LANEH) School of Life SciencesEast China Normal UniversityShanghai 200241China State Key Laboratory of Animal Nutrition Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193China

Background Soybean(Glycine max)meal is one of the important protein sources for fish,but the non-starch polysaccharides(NSP)in soybean meal impair the intestinal barrier *** we aimed to investigate whether xylanase can alleviate the adverse effects on the gut barrier induced by soybean meal in Nile tilapia and to explore the possible *** Nile tilapia(Oreochromis niloticus)(4.09±0.02 g)were fed with two diets including SM(soybean meal)and SMC(soybean meal+3,000 U/kg xylanase)for 8 *** characterized the effects of xylanase on the gut barrier,and the transcriptome analysis was performed to investigate the underlying *** xylanase improved intestinal morphology and decreased the concentration of lipopolysaccharide(LPS)in *** results of transcriptome and Western blotting showed that dietary xylanase up-regulated the expression level of mucin2(MUC2)which may be related to the inhibition of protein kinase RNA-like endoplasmic reticulum kinase(perk)/activating transcription factor 4(atf4)signaling *** analysis showed that addition of xylanase in soybean meal altered the intestinal microbiota composition and increased the concentration of butyric acid in the ***,dietary sodium butyrate was supplemented into the soybean meal diet to feed Nile tilapia,and the data verified that sodium butyrate mirrored the beneficial effects of *** Collectively,supplementation of xylanase in soybean meal altered the intestinal microbiota composition and increased the content of butyric acid which can repress the perk/atf4 signaling pathway and increase the expression of muc2 to enhance the gut barrier function of Nile *** present study reveals the mechanism by which xylanase improves the intestinal barrier,and it also provides a theoretical basis for the application of xylanase in aquaculture.

关键词： Butyrate Exogenous enzymes Intestinal health Intestinal microbiota Mucin xylanase

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Interaction between xylanase and a proton pump inhibitor on broiler chicken performance and gut function

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Animal Nutrition 2022年第1期8卷 277-288页

作者： Gemma Gonzalez-Ortiz Sophie A.Lee Kirsi Vienola Kari Raatikainen German Jurgens Juha Apajalahti Michael R.Bedford AB Vista MarlboroughWiltshireSN84ANUnited Kingdom Alimetrics Ltd. Koskelontie 19 BEspooFinland

Three hundred thirty-six Ross 308 male broiler chicks were used in a 21-d study to explore performance and gut function when treated with a proton pump inhibitor(PPI;0 or 89 mg/kg)in a 2×2 factorial arrangement with a xylanase(Xyl;0 or 0.1 g/kg)to determine if the beneficial activity of arabinoxylan(AX)depolymerisation,through arabinoxylo-oligosaccharides(AXOS)production,starts in the upper gastrointestinal *** with the PPI started from d 14,and by d 21 animal performance had deteriorated(Pxylanase even under acidic *** data suggest xylanase may function in the gizzard even though pH is not optimal for activity and emphasises the importance of chlorohydric acid secretions in ensuring overall optimum gut *** depolymerisation benefits animal performance although it is still unknown how the AXOS produced with xylanase supplementation in the upper gastrointestinal tract influence the microbial populations and overall gut functionality.

关键词： xylanase Proton pump inhibitor Arabinoxylan degradation Protein fermentation Broiler chicken

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Effects of xylanase,protease,and xylo-oligosaccharides on growth performance,nutrient utilization,short chain fatty acids,and microbiota in Eimeria-challenged broiler chickens fed high fiber diet

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Animal Nutrition 2023年第4期15卷 430-442页

作者： Yang Lin Jeferson M.Lourenco Oluyinka A.Olukosi Department of Poultry Science University of GeorgiaAthensGA 30602USA Department of Animal and Dairy Science University of GeorgiaAthensGA 30602USA

A 21-d experiment was conducted to study the effect of xylanase,protease,and xylo-oligosaccharides on growth performance,nutrient utilization,gene expression of nutrient transporters,cecal short-chain fatty acids(SCFA),and cecal microbiota profile of broilers challenged with mixed Eimeria *** study utilized 392 zero-d-old male broiler chicks allocated to 8 treatments in a 4×2 factorial arrangement,as follows:corn-soybean meal diet with no enzyme(Con);Con plus xylanase alone(XYL);Con plus xylanase combined with protease(XYL+PRO);or Con plus xylo-oligosaccharides(XOS);with or without Eimeria *** were based on a high-fiber(100 g/kg soluble fibers and 14 g/kg insoluble fibers)basal *** d 15,birds in challenged treatment were gavaged with a solution containing Eimeria maxima,Eimeria acervulina,and Eimeria tenella *** d 21,birds were *** depressed(P<0.01)growth performance and nutrient utilization,whereas supplementation had no *** were significant Eimeria×supplementation interactions for the sugar transporters GLUT5(P=0.02),SGLT1(P=0.01),SGLT4(P<0.01),and peptide transporter PepT1(P<0.01)in jejunal *** challenge increased the expression of GM-CSF2(P<0.01)and IL-17(P=0.04)but decreased(P=0.03)IL-1βexpression in the cecal ***×supplementation interactions for cecal acetate,butyrate,and total SCFA showed that concentrations increased or tended to be greater in the supplemented treatments,but only in non-challenged *** challenged with Eimeria *** higher concentrations of isobutyrate(P<0.01),isovalerate(P<0.01),and valerate(P=0.02)in cecal *** challenge significantly(P<0.01)decreased the microbial richness and diversity,and increased(P<0.01)the proportion of Anaerostipes butyraticus,Bifidobacterium pseudolongum,and Lactobacillus *** conclusion,Eimeria infection depressed growth performance,nutrient utilization with regulating nutrient ***,Eimeria challenge s

关键词： Eimeria xylanase Protease Xylo-oligosaccharide Microbiome Broiler chicken

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Studies on Mutation Breeding of High-Yielding xylanase Strains by Low-Energy Ion Beam Implantation

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Plasma Science and Technology 2007年第2期9卷 248-251页

作者：李市场姚建铭余增亮 Food and Bioengineering Department Henan University of Science and Technology Luoyang 471003China Key Laboratory of Ion Beam BioengineeringInstitute of Plasma PhysicsChinese Academy of SciencesHefei 230031China Key Laboratory of Ion Beam Bioengineering Institute of Plasma PhysicsChinese Academy of SciencesHefei 230031China

As a new mutagenetic method, low-energy ion implantation has been used widely in many research areas in recent years. In order to obtain some industrial strains with high xylanase yield, the wild type strain Aspergillus niger A3 was mutated by means of nitrogen ions implantation （10 keV, 2.6× 10^14 ～ 1.56 × 10^15 ions/cm^2） and a mutant N212 was isolated subsequently. However, it was found that the initial screening means of the high-yielding xylanase strains such as transparent halos was unfit for first screening. Compared with that of the wild type strain, xylanase production of the mutant N212 was increased from 320 IU/ml to 610 IU/ml, and the optimum fermentation temperature was increased from 28 ℃ to 30 ℃.

关键词： ion implantation xylanase Aspergillus niger

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Thermostable ethanol tolerant xylanase from a cold-adapted marine species Acinetobacter johnsonii

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Chinese Journal of Chemical Engineering 2019年第5期27卷 1166-1170页

作者： Dongsheng Xue Xuhao Zeng Dongqiang Lin Shanjing Yao Key Laboratory of Fermentation Engineering (Hubei Universty of Technology) Ministry of EducationHubei Provincial Cooperative Innovation Center of Industrial FermentationHubei Key Laboratory of Industrial MicrobiologyHubei University of TechnologyWuhan 430068China Department of Chemical and Bioengineering Zhejiang UniversityHangzhou 310027 China

A xylanase-producing bacterium, isolated from deep sea sediments, was identified as the cold-adapted marine species Acinetobacter Johnsonii. A cold-adapted marine species Acinetobacter Johnsonii could grow at 4 ℃. The optimum temperature and pH of xylanase from a cold-adapted marine species Acinetobacter Johnsonii were 55 ℃ and pH 6.0. xylanase from a cold-adapted marine species Acinetobacter Johnsonii remained at 80% activity after incubation for 1 h at 65 ℃. The xylanase activity was 1.2-fold higher in 4% ethanol solution than in ethanol free solution. Gibbs free energy of denaturation, ΔG, was higher in 4% ethanol solution than in ethanol free solution. Thermostable ethanol tolerant xylanase was valuable for bioethanol production by simultaneous saccharification and fermentation process with xylan as a carbon source.

关键词： xylanase Ethanol tolerant Thermostable Cold-adapted Acinetobacter Johnsonii

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Expression and Characterization of a Thermostable xylanase Gene xynA from a Themophilic Fungus in Pichia pastoris

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Agricultural Sciences in China 2011年第3期10卷 343-350页

作者： ZHAO Nan GUO Run-fang YU Hong-wei, KE Xiao-jing, JIA Ying-min KE Xiao-jing JIA Ying-min BAIYu Department of Bioengineering College of Food Science and Technology Hebei Agricultural University Baoding 071001 P.R.China

The gene of xylanase （xynA） was amplified by RT-PCR from the total RNA of a themophilic fungus Thermomyces lanuginosus SY2. The sequence analysis showed that gene coding region of mature peptide contained 0.585 kb, which coded 194 amino acids. The putative amino acid sequence and DNA sequence of xylanase from T. lanuginosus SY2 （GenBank no.： GU166389） were 98.97 and 99.49% identical to the other T. lanuginosus （GenBank no.： U35436）. A recombinant plasmid pPIC9K-xynA was constructed by inserting gene xynA into Pichia pastoris secretory vector pPIC9K. Linearized pPIC9K-xynA was transformed into P. pastoris GS115 with the method of electroporation. The recombinant strain was identified by G418 selection and confirmed by PCR analysis. It was induced by 1.0% methanol at 28°C to express the recombinant xylanase. The results showed that the recombinant xylanase was secreted into extracellular fermentation liquid. The highest enzyme activity of 113.5 IU mL-1 and protein content of 889.7 μg mL-1 were detected for 216 h of induction. The optimal pH value and temperature of the enzyme activity was 5.5 and 65°C, respectively. The xylanase activity retained above 80% from pH value 2.5 to 8.5 for 48 h. The enzyme activity was above 85% at incubation temperature of 55°C.

关键词： Thermomyces lanuginosus xylanase Pichia pastoris expression

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Effects of cellulase and xylanase enzymes mixed with increasing doses of Salix babylonica extract on in vitro rumen gas production kinetics of a mixture of corn silage with concentrate

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Journal of Integrative Agriculture 2015年第1期14卷 131-139页

作者： Abdelfattah Z M Salem German Buendía-Rodríguez Mona M M Elghandour María A Mariezcurrena Berasain Francisco J Pea Jiménez Alberto B Pliego Juan C V Chagoyán María A Cerrillo Miguel A Rodríguez Faculty of Veterinary Medicine and Animal Science Autonomous University of the State of Mexico National Center of Disciplinary Research in Animal Physiology and Improvement INIFAP Ajuchitlán Institute of Agricultural Sciences Autonomous University of HidalgoEx-Hda. of Aquetzalpa Faculty of Veterinary Medicine and Animal Science Juárez University of the Durango State

An in vitro gas production （GP） technique was used to investigate the effects of combining different doses of Salix babylonica extract （SB） with exogenous fibrolytic enzymes （EZ） based on xylanase （X） and cellulase （C）, or their mixture （XC; 1：1 v/v） on in vitro fermentation characteristics of a total mixed ration of corn silage and concentrate mixture （50：50, w/w） as substrate. Four levels of SB （0, 0.6, 1.2 and 1.8 mL g-1 dry matter （DM）） and four supplemental styles of EZ （1 μL g-1 DM; control （no enzymes）, X, C and XC （1：1, v/v） were used in a 4×4 factorial arrangement. In vitro GP （mL g-1 DM） were recorded at 2, 4, 6, 8, 10, 12, 24, 36, 48 and 72 h of incubation. After 72 h, the incubation process was stopped and supernatant pH was determined, and then filtered to determine dry matter degradability （DMD）. Fermentation parameters, such as the 24 h gas yield （GY24）, in vitro organic matter digestibility （OMD）, metabolizable energy （ME）, short chain fatty acid concentrations （SCFA）, and microbial crude protein production （MCP） were also estimated. Results indicated that there was a SBxEZ interaction （P〈0.0001） for the asymptotic gas production （b）, the rate of gas production （c）, GP from 6 to 72 h, GP2 （P=0.0095）, and GP4 （P=0.02）. The SB and different combination of enzymes supplementation influenced （P〈0.001） in vitro GP parameters after 12 h of incubation; the highest doses of SB （i.e., 1.8 mL g-1 DM）, in the absence of any EZ, quadratically increased （P〈0.05） the initial delay before GP begins （L） and GP at different incubation times, with lowering b （quadratic effect, P〈0.0001 ） and c （quadratic effect, P〈0.0001 ; linear effect, P=0.0018）. The GP was the lowest （P〈0.05） when the highest SB level was combined with cellulose. There were SBxEZ interactions （P〈0.001） for OMD, ME, the partitioning factor at 72 h of incubation （PF72）, GY24, SCFA, MCP （P=0.0143）, and pH （P=0.0008）. The OMD, ME, GY24 and SCFA with supplementation of SB extract at 1.8 mL g-1 DM were h

关键词： cellulase degradability gas production Salix babylonica xylanase

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Dietary soluble non-starch polysaccharide level and xylanase supplementation influence performance,egg quality and nutrient utilization in laying hens fed wheat-based diets

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Animal Nutrition 2021年第2期7卷 512-520页

作者： Xa H.Nguyen Hong T.Nguyen Natalie K.Morgan University of New England School of Environmental and Rural ScienceArmidaleNew South Wales2351Australia

The aim of this study was to evaluate the effects of dietary soluble non-starch polysaccharide(sNSP)content and xylanase supplementation on production performance,egg quality parameters,and nutrient di-gestibility in Hy-line Brown layers from 25 to 32 wk of age.A total of 144 Hy-line Brown laying hens(25 wk of age)were randomly allocated to 1 of 4 wheat-based dietary treatments in a 2×2 factorial experimental design,with 36 replicates of individual hens per *** diets were formulated to contain either a high or low sNSP level(at 13.3 or 10.8 g/kg)and were supplemented with either 0 or 12,000 BXU/kg exogenous *** were fed these treatment diets for an 8-wk period,and hen production perfor-mance,including daily egg production,average egg weight,daily egg mass,feed conversion ratio and pro-portion of dirty and abnormal eggs were measured at bird age 25 to 28 wk and 29 to 32 *** interaction between sNSP content of the diet and xylanase supplementation was observed on daily egg production from 25 to 28 wk of age(P-0.018);birds fed the high sNSP diet without xylanase had lower egg production than those fed any other *** interaction between the 2 dietary factors was also observed on hen weight gain at 29 to 32 wk of age(P=0.014),with birds fed the low sNSP diet with 12,000 BXU/kg xylanase presenting greater weight gain compared to those fed the high sNSP diet with 12,000 BXU/kg *** intake at 29 to 32 wk of age was reduced by xylanase supplementation(P=0.047).xylanase supplemen-tation also increased yolk colour score at both 28 and 32 wk of age,and decreased yolk weight at 32 wk of age(P-0.014,0.037 and 0.013,respectively).Birds fed the low sNSP diet presented lower protein digestibility(P-0.024)than those fed the high sNSP ***,birds fed high sNSP presented higher shell reflectivity at both 28 and 32 wk of age(P-0.05 and 0.036,respectively).The influence of duration of feeding the treatment diets on egg quality was also

关键词： Soluble NSP xylanase Laying hen Production performance Egg quality

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Mutation-Screening in xylanase-Producing Strains by Ion Implantation

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Plasma Science and Technology 2005年第1期7卷 2697-2700页

作者：李市场吴敏姚建铭潘仁瑞余增亮 Key Laboratory of Ion Beam Bioengineering of Institute of Plasma Physics Chinese Academy of Sciences Hefei 230031 China

With ion implantation (N+, energy 10 keV and dosage 1.56×1015 N+cm-2), a high xylanase-producing strain Aspergillus niger N212 was selected. Based on an orthogonal experiment, an optimal fermentation condition was designed for this high-yield strain. The suitable medium was composed of 8% corncob; 1.0% wheat bran; 0.1%TWEEN20; 0.5% (NH4)2SO4; 0.5%NaNO3; 0.5%FeSO4, 7.5 × 10-4; MnSO4·H2O, 2.5 × 10-4; ZnSO4, 2.0 × 10-4; CoCl2, 3.0 × 10-4. At present, under our experiment condition, xylanase activity of Aspergillus niger N212 reached a level of 600 IU/ml, almost increased by 100% in xylanase production and the time of yielding xylanase was largely reduced to 12 h at 28℃.

关键词： aspergillus niger ion implantation xylanase screening orthogonal experiment

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Evaluation of xylanase and a fermentable xylo-oligosaccharide on performance and ileal digestibility of broiler chickens fed energy and amino acid deficient diets

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Animal Nutrition 2021年第2期7卷 488-495页

作者： Gemma González-Ortiz Tiago T.dos Santos Michael R.Bedford AB Vista 3Woodstock CourtMarlborough Business ParkMarlboroughWiltshireSN84ANUK

The objective of this study was to evaluate the effect of a product consisting of a combination of xylanase and xylo-oligosaccharide(STBIO)on performance and ileal digestibility of broiler chickens fed energy and amino acid(AA)deficient ***-old male Ross 308 broiler chicks were randomly allocated to 8 pens per treatment,with 25 chicks per *** based on wheat-corn-soybean meal diets were arranged in a 3×2 factorial design:a positive control that met or exceeded nutrient recommendations(PC),a negative control diet with a 50 kcal/kg apparent metabolizable energy(AME)reduction(NC1)and NC1 with a 3%reduction in AA content(NC2),each with or without supplementation of 100 g/t of the *** weight gain(BWG),feed intake,feed conversion ratio corrected for mortality(FCR)and the European production efficiency factor(EPEF)were recorded from 0 to 42 *** d 42,ileal samples were collected to determine dry matter(DM),organic matter(OM),ash,protein and energy digestibility.A significant interaction was observed for BWG and feed intake(P<0.001).The energy and AA reduction reduced(P<0.05)BWG when compared to the *** effect of STBIO on BWG was greater in NC1(+451 g/bird)than in NC2(+314 g/bird)or PC(+176 g/bird)diets(P<0.05),and that in NC2 with STBIO was equal to that in PC without STBIO,and that in NC1 with STBIO was equal to that in PC with *** interactions were observed on the EPEF or FCR;however,STBIO improved EPEF(P<0.001)and FCR(P<0.001)irrespective of the energy reduction or AA *** intake of digestible DM,OM,ash and energy for the finisher period was increased with STBIO supplementation(P<0.01).A significant interaction was observed for the intake of digestible ***1 and NC2 reduced the intake of digestible protein;however,when STBIO was supplemented,it was improved in both diets to similar levels to the *** stimbiotic supplementation improved performance of broiler chickens fed all diets,particularly those deficient in AME and AA.

关键词： xylanase Fermentable oligosaccharide Broiler Stimbiotic

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