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Induction of Silencing Effect of Swedish Mutant Amyloid Precursor Protein by RNA interference

Journal of Huazhong University of Science and Technology(Medical Sciences) 2006年第4期26卷 399-401页

作者：邱昕陈国华潘纪安陈宇张苏明 Department of Neurology Integrated Traditional Chinese and Western Medicine HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Modern Virology Research Center College of Life SciencesWuhan UniversityWuhan430072China Department of Neurology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

Summary： Over-expression of app and Swedish mutation could cause some familial early onset AD. In this study, a primary screening was conducted of effective small interference RNAs （siRNAs） targeted wild type app （appwt） and Swedish mutant app （appswe）. One siRNA targeting appwt and the other siRNA targeting appswe were designed, All these siRNAs were endogenously expressed by siRNAs expressing plasmids, COS-7 cells were transiently co-transfected with app-GFP recombinant plasmids and siRNA expression vector, The silencing effect of each siRNA was quantitatively assessed by the level of expression of green fluorescent protein （GFP）. It was found that the siRNAs silenced appwt and appswe to different degrees, siRNA directed against appswe was more effective in suppressing the expression of fusion gene of appswe than that of appwt. The silencing effect of siRNA directed against appswe indicating allele-specific silencing property of the siRNAs. Therefore, siRNAs directed against app play an important role both in the therapeutic study of Alzheimer disease and functional exploration ofapp gene.

关键词： amyloid precursor protein small interference RNA wild type app Swedish mutant app

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