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One-step and wash-free multiplexed immunoassay platform based on bioinspired photonic barcodes

再生工程(英文) 2023年第3期4卷 238-244页

作者： Dagan Zhang Yuze Wang Junqi Zhao Xueqin Li Yuanyang Zhou Sen Wang Department of Andrology Nanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical SchoolNanjing 210008China Department of Clinical Laboratory The Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing210008China

Multiplex,rapid and accurate virus quantification plays a great value in biomedical ***,a novel one step,wash-free immunoassay platform based bioinspired PhC barcodes for multiplexed virus quantification was *** barcodes were decorated with PDA by self-polymerization of DA,thus this nanocomposite hybridized PhC barcodes facilitated the adsorption of FITC labelled antibodies and quenched itself photolumines-cent,allowing a fast responsive composite *** the presence of target analyte,the FITC-labelled detection antibody was released from the surface of PDA decorated microcarrier to specifically bind to the target ana-lyte,thus recovered the *** addition,the PhC microcarrier was enabled to carry out various color barcode for different targets detection though tuning internal periodic *** on these excellent performances of the nanocomposite barcode,this method can not only capture H1N1,H5N1,SARS-CoV-2 si-multaneously with rapid,accuracy but also accomplish multiplex quantification detection with ***,our developed platform was also achieved with high-sensitivity and high-specificity through the verification of clinical samples,thus laying out a new avenue for multiplex virus detection in clinical diagnosis.

关键词： Barcode Photonic crystals wash-free Immunoassay

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A pH-insensitive near-infrared fluorescent bioprobe for wash-free lysosome-specific tracking with long time during physiological and pathological processes

A pH-insensitive near-infrared fluorescent bioprobe for wash...

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第十一届全国化学生物学学术会议

作者： Zheng-Yin Pan Lu-Si Rao Xi-Juan Chao Cai-Ping Tan Liang-Nian Ji Zong-Wan Mao MOE Key Lab of BISC School of ChemistrySun Yat-Sen University

A novel dicyanomethylene-4 H-pyran based lysosome-specific near-infrared（NIR） bioprobe, DCM-ML, is reported. It exhibits very weak fluorescence（Ф = 0.007） in aqueous solution due to the energy relaxation from intramolecular rotation;but a large "off-on" NIR emission（20-folds） after specifically labeling lysosomes due to the local high viscosity instead of the local pH. Therefore, the imaging ability of DCM-ML will not confront the problems suffered by the currently used and reported pH-dependent lysosome probes（e.g., once lysosomal pH increases, their fluorescence gets quenched）;The pH-independent feature of DCM-ML is further demonstrated to be a great advantage for tracing lysosomes’ movements in a relatively long time, even during cellular processes when lysosomes are suffered from external stimulations that induce pH increase and apoptosis. In addition to the characteristics of pH-insensitivity and "off-on" NIR emission, DCM-ML also possesses characteristics of excellent membrane permeability, high selectivity, low cytotoxicity, good photo-stability and large Stokes shift（＞ 200 nm）, which qualify it as a superior wash-free NIR probe for tracking dynamic changes of lysosomes under both physiological and toxicological conditions. Hence, we believe DCM-ML may find a great potential of application in the future. [1-2]

关键词： Near-infrared "off-on" wash-free lysosome-specific imaging p H-insensitivity long-term tracking

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Mild synthesis of ultra-bright carbon dots with solvatochromism for rapid lipid droplet monitoring in varied physiological processes

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Regenerative Biomaterials 2024年第3期3卷 30-42页

作者： Borui Su Dong Gao Nini Xin Kai Wu Mei Yang Shichao Jiang Yusheng Zhang Jie Ding Chengheng Wu Jing Sun Dan Wei Hongsong Fan Zhenzhen Guo National Engineering Research Center for Biomaterials College of Biomedical EngineeringSichuan UniversityChengduSichuan 610064China Institute of Regulatory Science for Medical Devices Sichuan UniversityChengduSichuan 610064China Department of Gastroenterology Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengdu 610o72China

Lipid droplets(LDs)participating in various cellular activities and are increasingly being *** imaging provides powerful tool for dynamic tracking of LDs,however,most current LDs probes remain inconsistent performance such as low Photoluminescence Quantum Yield(PLQY),poor photostability and tedious washing ***,a novel yellow-emissive carbon dot(OT-cD)has been synthesized conveniently with high PLQY up to 90%.Besides,OT-CD exhibits remarkable amphiphilicity and solvatochromic property with lipid-water partition coefficient higher than 2,which is much higher than most LDs *** characters enable OT-CD high brightness,stable and wash-free LDs probing,and feasible for in vivo ***,detailed observation of LDs morphological and polarity variation dynamically in different cellular states were recorded,including ferroptosis and other diseases ***,fast whole imaging of zebrafish and identifed LD enrichment in injured liver indicate its further feasibility for in vivo *** contrast to the reported studies to date,this approach provides a versatile conventional synthesis system for high-performance LDs targeting probes,combing the advantages of easy and high-yield production,as well as robust brightness and stability for long-term imaging,facilitating investigations into organelle interactions and LD-associated diseases.

关键词： lipid droplet probe carbon dots fluorescence imaging wash-free dynamic monitoring

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