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Production of Yam Tubers Using Seed Tubers from vitroplants Regenerated from Aerial Stems in the Yam Species Dioscorea alata (L.) and Dioscorea cayenensis-rotundata (L. & P.) in Côte d’Ivoire

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Advances in Bioscience and Biotechnology 2024年第3期15卷 184-194页

作者： Koffi Honoré Kouamé Kouakou Marius Konan Kouadio Ignace Kouassi Kouablan Edmond Koffi Assanvo Simon-Pierre N’Guetta Department of des Science and Technology University Alassane Ouattara of Bouaké Bouaké C&#244te d’Ivoire Plant Production Research Unit Department of Natural Sciences University Nangui Abrogoua Abidjan C&#244te d’Ivoire Central Biotechnology Laboratory National Center for Agronomic Research (NCAR) Abidjan C&#244te d’Ivoire Genetics Laboratory of Bioscience Research Unit University Félix Houphou&#235t Boigny (UFHB) Abidjan C&#244te d’Ivoire

The aim of this study is to produce large quantities of yam tubers in the field from seed tubers derived from vitroplants regenerated from aerial stem cuttings and farmers’ tuber seeds. Seed tubers from ten yam cultivars, including five from Dioscorea alata (cDa083, cDa053, cDa115, cDa150 and cDa266) and five from Dioscorea cayenensis-rotundata (cDr015, cDr027, cDr150, cDr206 and cDr148) with distinct agro-morphological characteristics, were used as material. A completely randomized block design with 3 replications was used. In each block, all cultivars and seed types were represented. When put into cultivation, the tubers produced by the vitroplants all germinated and gave 100% healthy plants, compared with 86% to 100% healthy plants for the seed tubers produced by the farmers. Yields per hectare ranged from 10 to 25 tonnes for seed tubers produced by in vitro plants, and from 4 to 9 tons for seed tubers produced by farmers. Seed tubers from vitroplants can therefore be recommended to farmers as a solution to the problem of seed tuber unavailability.

关键词： Yams vitroplants Seed Tubers Yields

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Effect of Cultural Practices on the Growth of Date Palm vitroplants Planted in Errachidia （South East of Morocco）

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Journal of Life Sciences 2017年第6期11卷 279-285页

作者： Reda Meziani Chakib Alem Abdelhi Dihazi Brahim Rabach Mustapha Ait Chitt Fatima Jaiti Laboratoire National de culture des tissus de palmier dattier lnstitut National de la Recherche Agronomique Errachidia 52000 Morocco Equipe Biochimie des Ressources Naturelles Universitd My lsmail Meknes Facultk des Sciences et Techniques Errachidia 52000Morocco Laboratoire de Biotechnologie de la Valorisation et de la Protection des Agroressources UniversitO Cadi Ayyad Facult~ desSciences et Techniques Marrakeeh 40000 Morocco Equipe Protection Amdlioration et dcophysiologie vegetales Universite My Ismail Meknes Faculte des Sciences et Techniques Errachidia 52000 Morocco Laboratoire de Biotechnologie Domaine El Bassatine Meknes 50020 Morocco

In vitro multiplication of date palm is a very promising way for the rehabilitation of Moroccan palm devastated. The objective of this work was to study the development of vitroplants introduced into traditional and modem palm of the Errachidia region. The procedure followed is to make descriptive diagnoses in different sites where the palm was introduced and to evaluate the mortality of plants and cultural practices. The mortality rates varied from 78% to 19% in new plantation depending on the cultivation technique used. At the traditional palm, the rate of recovery ofplantlets was zero. The mortality is higher in Nejda cultivar compared to Boufegouss. This variety also presented a better adaptation in terms of shoot height in comparison with the development of Nejda and Mejhoul varieties. The production of offshoots as well as inflorescences takes place three years after plantation. The importance of improving cultivation techniques in date palm for the development of this sector in Morocco is discussed.

关键词： Date palm cultural practices vitroplants Phoenix dactylifera.

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High Light Intensity Increases the CAM Expression in “MD-2” Micro-Propagated Pineapple Plants at the End of the Acclimatization Stage

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American Journal of Plant Sciences 2015年第19期6卷 3109-3118页

作者： René C. Rodríguez-Escriba Romelio Rodríguez Dariel López Gustavo Y. Lorente Yaima Pino Carlos E. Aragón Yolanda Garza Florencio E. Podestá Justo L. González-Olmedo Laboratorio de Agrobiología Centro de Bioplantas Universidad de Ciego de ávila Ciego de ávila Cuba Departamento Biotecnología Facultad Ciencias Químicas Universidad Autónoma de Coahuila Coahuila México Centro de Estudios Fotosintéticos y Bioquímicos Facultad de Ciencias Bioquímicas y Farmacéuticas Universidad Nacional de Rosario Rosario Argentina

This work describes the evaluation of morpho-physiological and biochemical changes in “MD-2” micro-propagated pineapple plants (Ananas comosus (L.) Merr.) grown after 30 days under low light intensity (LL, greenhouse light conditions at 250 μmol·m-2·s-1) or high light intensity (HL, field light conditions at 800 μmol·m-2·s-1). Gas exchange, leaf pH, protein content and superoxide dismutase activity (SOD) (EC 1.***.1.1) were measured every 3 h during one day. Chlorophylls content and succulence index (SI) were determined at 9 h. Results showed significant differences in CO2 exchange rates, with a maximum occurring at 6 h (3.00 and 8.25 μmol CO2 m-2·s-1 for leaves under LL and HL conditions respectively). Plants under HL conditions had higher CO2 uptake and lower pH values between 0 h and 6 h respective to LL plants. The maximum pH value was attained 3 h before in HL plants. Leaf SI was increased and chlorophyll content decreased by HL conditions. SOD activity was higher in plants under HL conditions, near doubling those of LL plants at 18 h (2.8 versus 1.5 U·mg-1 Protein respectively). Both groups showed a typical CAM phenotype, but it was stronger in HL conditions, which may confer these plants with a better acclimation to transfer to the field.

关键词： Crassulacean Acid Metabolism Hardening Stage Ex Vitro vitroplants Gaseous Exchange

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