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Journal of Integrative Agriculture 2012年第8期11卷 1327-1332页

作者： WANG Mou ZHANG Ya YU Hui LAI Xin-sheng ZHU Jin-long JIAO Jin-zhen LAN Xian-yong LEI Chu-zhao ZHANG Liang-zhi CHEN Hong Shaanxi Key Laboratory of Molecular Biology for Agriculture College of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100P.R.China

visfatin, like insulin, induces phosphorylation of signal transduction proteins that operatate downstream of the insulin receptor. The present study is focused on detecting deletion of visfatin gene and analyzing its effect on growth traits in six Chinese cattle breeds （Nangyang, Luxi, Qinchuan, Jiaxian Red, Grassland Red, and Chinese Holstein） using DNA sequencing, PCR-SSCP and PCR-RFLP methods. For the first time, a 6-bp deletion of visfatin was described and two alleles were revealed：W and D. The χ2-test analysis demonstrated that all breeds were in agreement with Hardy-Weinberg equilibrium （P〉0.05）. The associations of the novel 6-bp deletion of visfatin gene with growth traits of Nanyang cattle at 6-, 12-, 18-, and 24-mon-old were analyzed. Birth weight, 12- and 24-mon-old cattle with genotype WW had greater birth weight and average daily gain than genotype WD （P〈0.01 or P〈0.05）. These results suggest that the deletion may influence the birth weight and bodyweight in 12 mon-old cattle.

关键词： visfatin deletion mutation cattle

Role of visfatin in obesity-induced insulin resistance

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World Journal of Clinical Cases 2022年第30期10卷 10840-10851页

作者： Mona Mohamed Ibrahim Abdalla Physiology Department Human Biology DivisionSchool of MedicineInternational Medical UniversityKuala Lumpur 57000Bukit JalilMalaysia

The growing worldwide burden of insulin resistance(IR) emphasizes the importance of early identification for improved ***,particularly visceral obesity,has been a key contributing factor in the development of *** obesity-associated chronic inflammatory state contributes to the development of obesity-related comorbidities,including ***,which are released by adipose tissue,have been investigated as possible indicators of *** was one of the adipocytokines that attracted attention due to its insulinmimetic *** is released from a variety of sources,including visceral fat and macrophages,and it influences glucose metabolism and increases *** relationship between visfatin and IR in obesity is *** a result,the purpose of this review was to better understand the role of visfatin in glucose homeostasis and to review the literature on the association between visfatin levels and IR,cardiovascular diseases,and renal diseases in obesity.

关键词： Homeostatic model assessment for insulin resistance Insulin resistance Obesity visfatin pre-B cell colony enhancing factor Nicotinamide phosphoribosyl-transferase enzyme Diabetes Adipocytokines

Plasma levels of omentin-1 and visfatin in senile patients with coronary heart disease and heart failure

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Asian Pacific Journal of Tropical Medicine 2014年第1期7卷 55-62页

作者： Xiu-Hua Wang Ling-Zhi Dou Can Gu Xiao-Qing Wang School of Nursing Central South University Second Hospital of Xiangya Central South University

Objective:To investigate the alteration of plasma levels of omentin-1 and visfatin in elderly patients with coronary heart disease(CHD)and heart ***:Plasma omentin-1 and visfatin levels were measured in 90 subjects(29 stable angina pectoris(SAP)cases,30 unstable angina pectoris(UAP)cases and 31 age-and sex-matched healthy controls(age≥60 years)by enzyme-linked immunosorbent assay *** to the New York Heart Association classification.59 CHDs were divided into three groups:functional I class,11 cases;functional II/III class,36 cases;and functional IV class,12 ***:The plasma level of omentin-1in CHO patients was significantly lower than thai of the control ***-lin SAP group and UAP group were significantly lower compared to the control group(there was no statistical significance between UAP group and SAP group;P>0.05).The plasma level of visfatin in CHD patients was significandy higher than that of the control ***,visfatin in SAP group and UAP group were all significantly higher compared to the control group,while there was no statistical significance between UAP group,and SAP *** plasma omentin-1 level was negatively correlated with SBP(r=-0.264,Pvisfatin level was positively correlated with TC(r=0.292,Pvisfatin levels(r=-0.280,Pvisfatin levels might be affected by HDL-c *** regression analysis showed that visfatin could be an independent risk factor of ***:Decreased levels of omentin-1 and increased levels of visfatin may be involved in the occurrence and development of ***-1 and visfatin,independently,may be protective and pro-inflammatory ***,both omentin-1 and visfatin may

关键词： Coronary heart disease Omenlin-1 visfatin NYHA class Stable angina pectoris Unstable angina pectoris

visfatin Protects Rat Pancreatic β-cells against IFN-γ-Induced Apoptosis through AMPK and ERK1/2 Signaling Pathways

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Biomedical and Environmental Sciences 2015年第3期28卷 169-177页

作者： XIANG Ruo Lan MEI Mei SU Yun Chao LI Li WANG Jin Yu WU Li Ling Department of Physiology and Pathophysiology School of Basic Medical SciencesPeking University Health Science Center and Key Laboratory of Molecular Cardiovascular SciencesMinistry of Education Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology

Objective Interferon-γ （IFN-γ） plays an important role in apoptosis and was shown to increase the riskof diabetes. visfatin, an adipokine, has anti-diabetic, anti-tumor, and regulating inflammatoryproperties. In this study we investigated the effect of visfatin on IFN-γ-induced apoptosis in ratpancreatic β-*** The RINm5F （rat insulinoma cell line） cells exposed to IFN-γ were treated with or withoutvisfatin. The viability and apoptosis of the cells were assessed by using MTT and flow cytometry. Theexpressions of mRNA and protein were detected by using real-time PCR and western blot *** The exposure of RINm5F cells to IFN-γ for 48 h led to increased apoptosis percentage of thecells. visfatin pretreatment significantly increased the cell viability and reduced the cell apoptosisinduced by IFN-γ. IFN-γ-induced increase in expression of p53 mRNA and cytochrome c protein,decrease in mRNA and protein levels of anti-apoptotic protein Bcl-2 were attenuated by visfatinpretreatment. visfatin also increased AMPK and ERK1/2 phosphorylation and the anti-apoptotic actionof visfatin was attenuated by the AMPK and ERK1/2 *** These results suggested that visfatin protected pancreatic islet cells against IFN-γ-inducedapoptosis via mitochondria-dependent apoptotic pathway. The anti-apoptotic action of visfatin ismediated by activation of AMPK and ERK1/2 signaling molecules.

关键词： visfatin IFN-γ Pancreatic β-cell Apoptosis AMPK ERK1/2

visfatin Gene Responses to 8 Weeks of Treadmill Running with or without <i>Pistachio atlantica</i>Liquid Extraction in Female Rat Tissues

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Food and Nutrition Sciences 2012年第8期3卷 1144-1149页

作者： Abbass Ghanbari-Niaki Navabeh Zare Kookandeh Saleh Rahmati Ahmadabad Rozita Fathi Department of Physical Education and Sport Science University of Mazandaran Babolsar Iran

visfatin, a novel adipokine, was revealed to be associated with obesity and to have insulin mimetic effect that is highly expressed in visceral adipose tissue. The aim of this study was to determine the liver and kidney visfatin relative gene expression. Twenty wistar rats (6-8 weeks old and 125-135 g weight) were used. Animals were randomly assigned into saline-control (SC), saline-training (ST), and Baneh-control (BC), and Baneh-training (BT). Training groups was given exercise on a motor-driven treadmill at 25 m/min (0% grade) for 60 min/day and 5 days/week for eight weeks. Subjects were fed oral, with Baneh extraction and saline for four weeks. visfatin relative gene expression was detected by Real-time PCR method. Also plasma was collected for glucose measurements. Results demonstrated that Baneh extraction significantly increase visfatin relative gene expression in liver (P visfatin relative gene expression in liver (P visfatin relative gene expression and Baneh increases visfatin relative gene expression in liver and kidney. Also Baneh can increases plasma glucose and liver glucose and glycogen concentration probably due to high fatty acid and component.

关键词： visfatin Female Rats Treadmill Exercise Pistachia atlantica

visfatin对SW872脂肪细胞胰岛素信号分子蛋白表达的影响

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武汉大学学报（医学版） 2013年第6期34卷 810-813,853页

作者：马新瑜卢慧玲李瑞珍温宇姚辉杨禄红秦原黄小力林汉华武汉市儿童医院放射科湖北武汉430016 华中科技大学同济医学院附属同济医院儿科湖北武汉430030 武汉市儿童医院内分泌科湖北武汉430016

目的:观察visfatin对胰岛素抵抗状态下的SW872成熟脂肪细胞胰岛素信号分子胰岛素受体底物-1(IRS-1)、胰岛素受体底物-2(IRS-2)和磷脂酰肌醇3-激酶(PI3K)蛋白表达的影响。方法:体外培养SW872前脂肪细胞,诱导细胞分化成熟,建立胰岛素抵抗... 详细信息

目的:观察visfatin对胰岛素抵抗状态下的SW872成熟脂肪细胞胰岛素信号分子胰岛素受体底物-1(IRS-1)、胰岛素受体底物-2(IRS-2)和磷脂酰肌醇3-激酶(PI3K)蛋白表达的影响。方法:体外培养SW872前脂肪细胞,诱导细胞分化成熟,建立胰岛素抵抗模型,用含有100nmol/L visfatin的无血清培养液孵育1h后收获细胞,采用Western印迹法检测visfatin刺激前后信号分子IRS-1、IRS-2和PI3K的蛋白表达水平。结果:在正常状态下(正常组内比较),visfatin促进脂肪细胞IRS-1、IRS-2和PI3K的蛋白表达,分别增加了51.65%(Pvisfatin刺激组的IRS-1、IRS-2和PI3K的蛋白表达水平,分别比基础状态组增加了26.98%(Pvisfatin通过调节脂肪细胞胰岛素信号分子IRS-1、IRS-2和PI3K蛋白表达而发挥促进葡萄糖转运、改善胰岛素抵抗的生物学作用。

关键词： visfatin SW872脂肪细胞胰岛素抵抗

visfatin levels in patients with severe pneumonia

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World Journal of Emergency Medicine 2011年第2期2卷 132-136页

作者： Xie Juan Yi-ming Lu Jin-dong Shi Xing-qi Deng Wei Long Department of Respiration Fifth People's Hospital Affiliated to Fudan University Shanghai 200240 China Emergency Department Ruijin Hospital Affiliated to Shanghai Jiao Tong University Shanghai China Department of Emergency Fifth People's Hospital Affiliated to Fudan University Shanghai 200240 China Emergency Department Central Hospital Minhang District Ruijin Hospital Shanghai China

BACKGROUND： As a cytokine highly expressed in internal organs, visfatin could be used as a biomarker of systemic inflammation response for chronic obstructive pulmonary diseases, but few studies have reported the use of visfatin in severe pneumonia. The present study was undertaken to determine the plasma levels of visfatin in patients with severe pneumonia. METHODS：A total of 70 patients, including 40 patients with severe pneumonia （group A） and 30 patients with non severe pneumonia （group B） who had been admitted to the ICU from June 2009 to June 2010, were enrolled in this prospective study. And another 30 healthy physical examinees served as healthy controls （group C）. Patients were excluded if they suffered from severe diseases of the heart, brain and kidney, cancers, autoimmune diseases, or received special treatment in the latest month. The plasma levels of visfatin, IL-6, IL-8 and TNF-α were measured by ELISA, while the level of CRP was determined by immuneturbidimetry, and the routine blood test was performed. Blood gas analysis and Acute Physiology and Chronic Health Evaluation II （APACHE II） were performed in patients with pneumonia. Comparisons between the groups were conducted by Student＇s t test, ANOVA or nonparametric test. Correlation analysis was carried out by Pearson＇s correlation test or Spearman＇s rank-order correlation test. RESULTS：The plasma level of visfatin in group A was significantly higher than that in groups B and C （P〈0.001）, and the level of visfatin in group B was significantly higher than that in group C （P〈0.001）. The plasma level of visfatin was positively correlated with CRP, TNF-α, APACHE II and PMN% in patients with severe pneumonia （rho=0.653, r=0.554, r=0.558, r=0.484, respectively, P〈0.05 for all）, while it was negatively correlated with PaO2 and PaO2/FiO2 （rho=-0.422, r=-0.543, respectively, P〈0.05 for all）. CONCLUSION：visfatin may be involved in the systematic inflammation response in patients with severe pneumonia as a

关键词： Severe pneumonia visfatin Interleukin-6 Interleukin-8 Tumor necrosis factor-a C-reactive protein Acute Physiology and Chronic Health Evaluation II (APACHE II) Granulocyte percent(PMN%)

visfatin levels are low in subjects with type 2 diabetes compared to age-matched controls

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Journal of Diabetes Mellitus 2012年第4期2卷 373-377页

作者： Subhashini Yaturu Jared Davis LaTonya Franklin Runhua Shi Prasanna Venkatesh Sushil K. Jain Department of Medicine Stratton VA Medical Center/AMC Albany USA Department of Research Overton Brooks VA Medical Center Shreveport USA Louisiana State University Health Sciences Center Shreveport USA Overton Brooks VA Medical Center/LSUHSC Shreveport USA

Background: Visceral adiposity correlates strongly with insulin resistance and components of metabolic syndrome. visfatin is preferentially produced by human visceral adipose tissue. Objective: The objective of this study was to evaluate the relationship between circulating levels of visfatin and other adipocytokines in patients with type 2 diabetes (DM) compared to the age-matched control subjects without DM. Methods: Anthropometric parameters, lipid profiles, high-sensitivity CRP (hs-CRP), insulin resistance [HOMA-IR], and levels of visfatin, TNF-α, and resistin were evaluated in 55 subjects with diabetes and 56 age matched control subjects without diabetes. Results: The visfatin levels were significantly lower in subjects with diabetes (p visfatin levels correlated strongly with resistin and CRP. We noted negative correlation of visfatin levels with BMI, triglycerides and glucose. Conclusion: These data suggest that visfatin may be a marker of subclinical inflammatory state.

关键词： visfatin Adiponectin Resistin Diabetes Insulin Resistance TNF-α BMI Metabolic Syndrome Visceral Adiposity

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visfatin与甲状腺功能紊乱研究进展

中国老年学杂志 2012年第1期32卷 201-204页

作者：张玲刘慧霞中南大学湘雅医院老年科湖南长沙410008

visfatin（内脂素）是2005年日本学者Fukuhara等〔1〕从人和大鼠脂肪组织中分离出的一种高表达mRNA,具有模拟胰岛素功能,并与代谢综合征的发生发展密切相关〔2〕。visfatin与前B细胞克隆增强因子（pre-B cell colony-enhancing factor,P... 详细信息

visfatin（内脂素）是2005年日本学者Fukuhara等〔1〕从人和大鼠脂肪组织中分离出的一种高表达mRNA,具有模拟胰岛素功能,并与代谢综合征的发生发展密切相关〔2〕。visfatin与前B细胞克隆增强因子（pre-B cell colony-enhancing factor,PBEF）5＇端基因结构一致。PBEF是早期阶段B淋巴细胞的生长因子,主要在骨髓、肝脏、肌肉中表达。

关键词： visfatin 前B细胞克隆增强因子烟酰胺磷酸核糖转移酶甲状腺功能紊乱

visfatin基因表达下调对小鼠脂肪细胞及肝细胞脂代谢的影响

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中国老年学杂志 2013年第1期33卷 77-79页

作者：张亚丽李伶杨刚毅卢春敏重庆医科大学检验系临床生化教研室教育部临床检验诊断学重点实验室重庆400016

目的探讨visfatin基因表达抑制对小鼠脂肪细胞及肝细胞脂代谢相关基因的影响。方法构建并筛选具有最佳抑制率的pGene-sil-visfatin表达载体,转染小鼠3T3-L1脂肪细胞及Hepa1-6肝细胞,Western印迹法测定两种细胞的visfatin蛋白水平,实时... 详细信息

目的探讨visfatin基因表达抑制对小鼠脂肪细胞及肝细胞脂代谢相关基因的影响。方法构建并筛选具有最佳抑制率的pGene-sil-visfatin表达载体,转染小鼠3T3-L1脂肪细胞及Hepa1-6肝细胞,Western印迹法测定两种细胞的visfatin蛋白水平,实时荧光定量PCR法测定Vis-fatin、脂代谢相关基因的mRNA表达水平,油红O染色法测定脂肪细胞内甘油三酯(TG)含量变化。结果 pGenesil-visfatin可有效抑制两种细胞Vis-fatin蛋白表达。脂肪细胞转染pGenesil-visfatin后,脂代谢相关基因PPARα/γ,HSL,ATGL,AP2,SREBP1,FAS,ACC mRNA表达均下降(其中PPARγ与ACC P0.05)。结论 visfatin基因表达下调可能对细胞脂质代谢具有调节作用。

关键词： visfatin 3T3-L1细胞 Hepa1-6细胞脂代谢